• Title/Summary/Keyword: 아미노산 조성

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Free Amino Acids of Xylem-Pith in Panax ginseng Root. (인삼근동체중심부의 유리아미노산)

  • Lee, Mee-Kyoung;Park, Hoon
    • Journal of Ginseng Research
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    • v.11 no.1
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    • pp.32-38
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    • 1987
  • Composition of free amino acids (FAA) in the central part (xylem plus piths,of tap root in P. ginseng was investigated in velation to stem status at harvest. The sum of FAA tended to be higher with dead stem than with healthy one but both were not significantly different. The sum of FAA (3.6-4.9% dried weight) was much less than total FAA, suggesting that water soluble nonprotein fraction contained large quantity of ninhydrih positive components except FAA. Pattern of amino acid composition between both stem status was not different. Ten of all 17 amino acids showed increasing tendency with dead stem and two, glutamic acid and cysteine, decreasing. Major FAA were arginine (relative content 58%), glycine (8.2), lysine (5.9), serine (5.7), glutamic acid (4.2) and aspartic acid (3.5). Above facts strongly suggest that the inside white of red ginseng did not closely related with FAA and that early defoliation or stem death did not decrease FAA. The content of arginine was heighest in all cases reported indicating the important role of nitrogen metabolism. Pattern of PAA composition except arginine was not different in present samples but greatly different with other cases reported mainly due to alanine, phenylalanine, glycine and proline.

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High Performance Liquid Chromatographic Analysis of Free Amino Acids in Various Ginseng Products (고속액체(高速液體)크로마토그래피에 의한 각종(各種) 인삼제품(人蔘製品)중의 유리아미노산 조성의 분석(分析))

  • Lee, Sung-Woo;Kurozaki, Toshiharu;Woo, Sang-Kyu;Yoon, Tai-Heon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.11 no.3
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    • pp.37-40
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    • 1982
  • Fifteen free amino acids except tryptophan, proline and cystine were identified from Korean red ginseng and dried ginsengs from Korea, America and Canada using by high performance liquid chromatography (HPLC). Arginine was 72.6% of total free amino acids in the red ginseng and 48.2 to 68.7% in the dried ginsengs. The content of each free amino acid was lower in the red ginseng than in Korean dried ginseng. Most free amino acids in Korean dried ginseng showed higher content than those in American and Canadian ones. Tryptophan, proline cystine, methionine and phenylalanine were not detected in the extracts of red ginseng and of Korean white ginseng. Arginine was highest in these extracts and all free amino acids were higher in the white ginseng extract.

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Comparison of amino acid and free amino acid contents between cap and stipe in Flammulina velutipes and Pleurotus ostreatus (팽이와 느타리버섯 자실체의 갓과 대에서 아미노산 및 유리아미노산 함량 비교)

  • Kim, Jung-Tae;Kim, Mi-Jung;Jhune, Chang-Sung;Shin, Pyung-Gyun;Oh, YounLee;Yoo, Young-Bok;Suh, Jang-Sun;Kong, Won-Sik
    • Journal of Mushroom
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    • v.12 no.4
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    • pp.341-349
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    • 2014
  • The composition and content of amino acid and free amino acid were separately compared in cap and stipe of fruitbody obtained from different color strains in winter mushroom and oyster mushroom strains. The result showed different kinds and amount of amino acids according to the mushroom parts, strains and kinds. Tryptophane was not detected in any mushrooms. Alanine was also not detected in all oyster mushroom strains. The content of glutamic acid was the highest and that of methionine, phenylalanine and cysteine were very low in all mushroom strains. When compared by parts, the ratio of amono acids showed similar tendency in composition. Generally the content of amono acids showed higher in caps than in stipes. The profile and content of free amino acid showed a lot of variation between cap and strip in the intra and inter mushrooms.

Compositions in Amino Acids and Nucleotides of Fermented Entrails of Yellow Corvina (조기속젓의 핵산관련물질(核酸關聯物質) 및 유리(遊離)아미노산(酸) 조성(組成))

  • Chung, Seung-Youg;Sung, Nak-Ju;Lee, Young-Kyoung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.13 no.3
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    • pp.285-290
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    • 1984
  • Changes of free amino acids, nucleotides and their related compounds during the fermentation of yellow corvina entrails, Pseudosciaena manchurica, were analyzed. In fresh extract, alanine, glutamic acid, serine, lysine and leucine were dominant amino acids, and content of arginine, aspartic acid, cystine and tyrosine were low. The free amino acids analyzed in this experiment were not changed in composition but changed in amounts during the fermentation of yellow corvina entrails. Alanine, glutamic acid, lysine and leucine were abundant in both fresh sample and fermented products. Content of ATP, ADP, AMP and IMP decreased while hypoxanthine increased during the fermentation of yellow corbina entrails. The total free amino acid nitrogen at 30 day fermentation was 71% of its extract nitrogen. It is believed that such amino acids as glutamic acid, alanine, lysine, valine, leucine, nucleotides and their related compounds as hypoxanthine play an important role as taste compounds in fermented yellow corvina entrails.

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On the Chemical Analysis of Amino Acid of Yellow Corvenia (참조기의 아미노산 분리정량)

  • 차월석;김종균김연순
    • KSBB Journal
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    • v.4 no.2
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    • pp.157-161
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    • 1989
  • The contents of total amino acid and free amino acid in the protein of yellow corvenia were analysed quantitively by HPLC. The results were as follow; The contents of free amino acid of yellow corvenia flesh and yellow covenia roe were 323.0mg/100g and 932.5mg/100g, respectively. The contents of total amino acids of yellow corvenia flesh and yellow covenia roe were 33,2620.0mg/100g and 64,861.0mg/100g, respectively. The contents of essential amino acid of yellow corvenia flesh and yellow corvenia roe were 12,196.0mg/100g and 13,143.0mg/100g, respectively.

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The Effect of Korean Soysauce and Soypaste Making on Soybean Protein Quality Part II. Chemical Changes During Meju-brine Ripening (재래식 간장 및 된장 제조가 대두 단백질의 영양가에 미치는 영향 제2보 메주장의 숙성중에 일어나는 성분 변화)

  • Lee, Cherl-Ho
    • Korean Journal of Food Science and Technology
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    • v.8 no.1
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    • pp.19-32
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    • 1976
  • The laboratory Mejus as well as home-made Meju and improved Meju received from Korea were ripened in the brine for up to 8 months and the changes is the chemical composition during the process were determined and the differences between the types of Meju were compared. On the basis of the amino acid pattern, the changes in the protein quality of soybean during the process was evaluated. No significant changes in the general chemical composition of Meju were noticed during the ripening for 8 months. However, the nitrogen solubility of Meju increased for $13{\sim}29%$ to $66{\sim}78%$ during 8 month ripening of the Meju-brine mixture. The concentration of free amino-N to the total-N increased from $4{\sim}7%$ in Meju to $29{\sim}35%$ in the 8month ripened mixture. The concentration of amino-N to the total-N increased from $1{\sim}4%$ in Meju to $5{\sim}14%$ in the 8month ripened mixture and the changes varied with the type of Meju used. Remarkable changes in the amino acid pattern of soybean were occured during the ripening process. The concentration of methionine decreased to the half of original Meju during the first month of ripening. Arginine and histidine were destroyed rapidly by the ripening longer than 1 month. A considerable amount of ornithine was synthesized during the ripening. The amino acid pattern of Meju did change drastically during the ripening longer than 3 months and the changes varied with the type of Meju. The retention of the nutrients in soybean during 8 month ripening of the laboratory 3 month Meju in the brine was 49% for carbohydrates, 107% for crude fat, 93% for crude protein and 74% for the total amino acid. Histidine, arginine and methionine and 74% for the total amino acid. Histidine, arginine and methionine were the most damaged during the process, retaining only 25%, 27% and 49% of the contents in raw soybean, respectively, whereas lysine retained 79%. By the separation of the 8 month ripened mixture, approximately 60% of crude protein, all of crude fat and 80% of carbohydrates in the mixture were retained in soypaste. Soypaste contained higher concentrations of amino acids per 16gN compared to soysauce, except for lysine. The most limiting amino acid of the protein was the S-containing amino acids in all cases studied, whereas the second limiting amino acid varied from valine in soybean to threonine in most of Mejus and the brine mixtures, lysine in most of soypastes and tryptophan in some of soysauces. According to the protein quality evaluation made by the reference of the FAO provisional pattern of amino acid, the chemical score of raw soybean was 82, which was reduced to 77 by cooking and further reduced to $71{\sim}74$ by Meju fermentation. At the eighth month of ripening the chemical score of the Meju-brine mixtures were reduced to $51{\sim}66. After the separation, the chemical score of soypaste ranged from 60 to 71, whereas that of soysauce varied from 45 to 57. Generally, the products made from improved Meju recorded the highest score, whereas those made from homemade Meju showed the poorest protein quality. The essential amino acid index(EAAI) of the samples was similar to the chemical score, but it appeared to fit the overall changes in the amino acid pattern during the process better than the chemical score.

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FREE AMINO ACID CONTENT IN THE EXTRACT OF CORAL DAMSELFISH, CHROMIS NOTATUS (자리돔 엑스분의 유리아미노산)

  • HA Jin-Hwan;LEE Eung-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.12 no.4
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    • pp.241-243
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    • 1979
  • Coral damselfish (demoiselle), Chromis notatus, has been one of the most tasty sea foods in Jeju-Do, Korea, The free amino acids in tile extracts of Chromis notatus were analysed by amino acid autoanalyser. In the extracts, taurine, lysine, glycine and alanine were abundant, holding $30.3\%,\;22.7\%,\;16.0\%\;,\;11.5\%$ of total amino acid content, respectively and followed threonine, glutamic acid and serine, in order. While methionine, histidine, leucine, isoleucine, valine were poor and aspartic acid and proline were trace in content.

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Purification and Characterization of Carotenoprotein from the Muscle of Ascidian, Halocynthia roretzi (우렁쉥이 육으로부터 Carotenoprotein의 분리.정제 및 특성)

  • Kang, Ok-Ju;Byun, Hee-Guk;Jeon, You-Jin;Kim, Jong-Bae;Kim, Se-Kwon
    • Applied Biological Chemistry
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    • v.41 no.1
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    • pp.31-41
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    • 1998
  • A carotenoprotein from the muscle of Ascidian (Halocynthia roretzi) was purified by ion exchange chromatography and gel filtration chromatography, and analyzed molecular weight, stability of pH and heat, effect of denaturing agents, amino acids and fatty acids composition. The purified carotenoprotein had absorption maxima of 463 nm and 439 nm. The carotenoprotein had an approxmimate molecular weight of 64.4 kDa in polyacrylamide gel electrophoresis. The amino acid compositions of carotenoprotein were mainly Gly (15.39%), Asn (11.31%), Gln (10.62%) and Ser (13.35%). The major fatty acids composition of carotenoprotein were $C_{16:1(n-7)}\;(15.4%)$, $C_{22:1(n-9)}\;(14.5%)$ and $C_{20:1(n-11)}\;(11.4%)$. The monounsaturated fatty acids (45.2%) contained abundant content compared to other saturated (38.1%) and polyunsaturated (11.7%) fatty acids.

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Fatty Acids, Amino Acids and Thermal Properties of Specialty Rice Cultivars (특수미 품종의 지방산과 아미노산 조성 및 열적 특성)

  • Choi, In-Duck
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.9
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    • pp.1405-1409
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    • 2010
  • The compositions of fatty acid and amino acid of specialty rice which includes colored rice (Heugjinju, Jeugjinju, Josangheugchalbyeo), flavored rice (Heughyangmi, Hyangmi1), and giant embryo rice (Keunnun) were determined and compared to those of regular rice (Ilpumbyeo, Whaseonchalbyeo). Major fatty acids were linoleic acid (C18:2) and oleic acid (C18:1), which were composed of 75~80% of total fatty acids. Major amino acids were glutamic acid and aspartic acid in most cultivars but Jeugjinju in which cysteine (169.61 nmol) and GABA (129.32 nmol) were the most abundant amino acids. Thermal properties measured by differential scanning calorimeter (DSC) revealed that the enthalpy (${\Delta}H$) for starch gelatinization was the highest in Josangheugchalbyeo and Whaseonchalbyeo. It suggests that the starch structure of waxy rice could be more crystallized compared to non-waxy rice, and also that amylopectin could have more impact on starch gelatinization than amylose. The on-set and complete temperature for starch gelatinization were higher in colored rice of Heugjinju and Jeugjinju, and regular rice of Whateonchalbyeo.

Characteristics of Chitosan-Silk fibroin composites (키토산과 견사 단백질 복합체의 특성)

  • 김희숙;원용돈;류병호
    • The Korean Journal of Food And Nutrition
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    • v.9 no.4
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    • pp.496-499
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    • 1996
  • In order to develop the functional diet food, characteristics of chitosan and silk fibroin composite was designed. Chitosan extracted from a prawn and silk fibroin was prepared from silkworm. The silk fibroin was dissolved rapidly in the 8M LiBr at a temperature of more than 4$0^{\circ}C$. Amino acid composition of fibroin composite revealed the same pattern that of native silk fibroin and regenerated silk fibroin. Predominant amino acid of chitosan-fibroin composite contained glycine, alanine, serine, tyrosine, threonine, and glutamic acid in order. According to the basis on the infrared spectrum, chitosan-silk fibroin composite is not distinguished differents composite ratio of chitosan and silk fibroin.

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