• Horticultural Science & Technology
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• v.16 no.2
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• pp.236-238
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• 1998

This study was conducted to investigate the effective media and optimal days after pollination for germination of seeds which were crossed by selfing or reciprocal pollination in Cymbidium spp. The elongation of ovary stopped about 110 days after pollination regardless of cross combinations. The increase of ovary width stopped about 140 days after pollination showing some differences among cross combinations. The cross combinations expressing high capsule number and viability showed good germination when they were sown in vitro. The capsules collected 150 to 180 days after pollination showed good germination performance and short days of germination. Hyponex media was the most effective for protocorm development and germination.

• FLOWER RESEARCH JOURNAL
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• v.18 no.4
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• pp.251-255
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• 2010

This study was conducted to investigate the detrimental effects of decapping and emasculation on the postharvest quality and the vase life of cut Cymbidium flowers with or without 1-Methylcyclopropene (1-MCP) and Aminoethoxyvinylglycine (AVG) treatments. The vase life treated by 1-MCP 150 ppb for four hours regardless of decapping or emasculation was significantly prolonged 12 days more. Ethylene production in the treatment without 1-MCP began to be raised from 7days, 1-MCP treatments, regardless of the concentration of processing did not occur until 15days. Ethylene began to production when the lip began to coloration. Compared to the 1-MCP effect, the AVG treatment of 0.5 and 1mM for four hours prolonged the vase life about nine days more. Whereas, the only decapping or emasculation treatments were effective to more three days longer in the vase life of cut Cymbidium' Halleluiah' flowers. 1-MCP treatment was very beneficial for Cymbidium 'Halleluiah' cut flowers than AVG.

• Korean Journal of Breeding Science
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• v.43 no.5
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• pp.399-404
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• 2011

Genetic diversity among 28 Cymbidium varieties was evaluated by using a sequence-related amplified polymorphism (SRAP) marker system. The SRAP marker which was based on the open reading frames (ORFs) regions was developed primarily for Brassica species, but has been applied to various crops. A total of 30 SRAP primer combinations were initially screened. Twenty-eight SRAP primer combinations showed high polymorphism among the 28 Cymbidium varieties, which were consisted of breeding varieties and their parents in National Institute of Horticultural & Herbal Science (NIHHS). The amplified DNA fragments were separated by denaturing acrylamide gels and detected silver staining method. One hundred ninety six polymorphic bands (7 per primer) were generated and ranged from 0.3 to 1.0 kb in size. Polymorphic fragments were scored for calculating simple matching coefficient of genetic similarity and cluster analysis with multi-variate statistical package (MVSP) 3.1. The mean genetic similarity coefficient value was 0.588. The results showed that the correlation between $F_1$ varieties and their parents was high. These studied SRAP markers will be useful tools for genotype identification, germplasm conservation, genetic relationships in Cymbidium.

• Korean journal of applied entomology
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• v.52 no.4
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• pp.403-408
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• 2013

A survey of pest occurrence and status of farmer's pest management was conducted at 45 cymbidium farms in 10 major cultivation areas in Korea. The pest species collected from the cymbidium farms were identified as follows: Tetranychus urticae Koch, Frankliniella intonsa Trybom, Pinnaspis aspidistrae Signoret, Incilaria confusa Cockarel, Halyomorpha brevis Walker, Myzus persicae S$\ddot{u}$lzer, and Aphis gossypii Glover, Coccus hesperidum Linnaeus, Thrips flavus Schrank, and Thrips tabaci Lindeman. The two-spotted spider mite, T. urticae, was the key pest in cymbidium production, occurring on 45 farms, followed by scales (20 farms), slugs (6), thrips (8), aphids (5), and stinkbug (1). PCR-RFLP of the rDNA ITS2 region revealed that two thrips species, Thrips flavus Schrank and Thrips tabaci Lindeman, occur on cymbidium farms. Therefore, it is necessary for the cymbidium farmers to establish an integrated pest management system to meet quarantine standards.

• Horticultural Science & Technology
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• v.18 no.4
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• pp.513-517
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• 2000

This experiment was carried out to detect the cymbidium mosaic virus (CymMV) and the odontoglossum ringspot virus (ORSV) in the seed-derived plantlets of Phalaenopsis imported from Taiwan by one-step reverse transcription-polymerase chain reaction (RT-PCR). Simple and rapid crude plant extracts for RT-PCR were prepared. The reverse transcription step was performed at $42^{\circ}C$ for 45 min and the following thermal cycling scheme was used for 36 reaction cycles: template predenaturation at $96^{\circ}C$ for 2 min, template denaturation at $96^{\circ}C$ for 30 s, primer annealing at $60^{\circ}C$ for 30 s, and DNA synthesis at $72^{\circ}C$ for 1 min. Of the 40 seed-derived plantlets of Phalaenopsis imported from Taiwan, all of them were infected with CymMV, but ORSV was not detected.

• Korean Journal of Breeding Science
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• v.43 no.6
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• pp.559-563
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• 2011

Cymbidium 'Purple Star' (Miake 'Pieta' ${\times}$ 'Allstar Mariane') was developed from a cross between hybrids at the National Institute of Horticultural & Herbal Science (NIHHS), Rural Development Administration (RDA) in 2008. A cross was made between purple colored flower C. Miake 'Pieta' as maternal line and dark pink colored flower, C. 'Allstar Mariane' as paternal line in 1995. The seed germination, cultivation, selection, and characteristic trials were conducted from 1996 to 2002. The line was named as Wongyo 'F1-21' and phenotype was characterized in 2007 as a new cultivar. The 'Purple Star' has dark purple basal color (RHS, GP186A) on both of sepal and petal with red lip (RHS, RP59A). 'Purple Star' has about 10.7 flowers per flower stalk and flower size of 7.6 cm. General appearance of petals and sepals is slightly incurved shape. The plant size is intermediate having erect peduncle. Blooming is started from the late of January (mid-winter) under optimal culture condition. Leaf attitude and twisting is erect and weak respectively. This hybrid has attractive floral arrangement, long flower stalk (78.4 cm) and vigorous growth. 'Purple Star' has a great potential for exporting to Chinese market.

• Korean Journal of Plant Resources
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• v.35 no.2
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• pp.362-371
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• 2022

This study was conducted to analyze the fragrance characteristics of Cymbidium 'Saelbit' and 'Midan' according to floral organs. As test materials, full bloom flowers were divided into four organs: sepal, petal, labellum, and column. Using the gas chromatography (GC) based electronic nose, fragrance patterns, intensity, and volatile components were analyzed. Principle component analysis (PCA) and discriminant factorial analysis (DFA) plots by electronic nose data showed that volatiles of both cultivars have a distinct difference in fragrance patterns according to the floral organs, and the value of fragrance distance and pattern discrimination index (PDI) between samples was significantly high between control and sepals in both cultivars. Among the main fragrance components, several components including nootkatone were detected in both cultivars and all floral organs. However, few components such as decane were found in specific cultivar or floral organs. These results will provide useful information to select suitable materials with desired fragrance and to enhance the utilization of domestic Cymbidium cultivars. In addition, considering the recent negative perception of artificial ingredients and the growing demand for natural materials, continuous researches on scent properties of promising cultivars are required.

• Journal of the Korean Society of Floral Art and Design
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• no.45
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• pp.3-12
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• 2021

The 1-methylcyclopropene (1-MCP) is the gaseous ethylene action inhibitor, which delay senescence and wilting by blocking the binding of its receptors. We investigated the effects of 1-MCP with different treated duration (0h (Control), 1h, and 4h) in cut flowers of Eustoma grandiflorum 'Voyage' and Cymbidium 'Lapine Hat'. The relative water uptake increased by 14.5% than control (9.8%) and the flower diameter maintained high from 3 to 11 days in 1-MCP treatment for 4h in E. grandiflorum 'Voyage'. The relative fresh weight was averagely high in 1-MCP treatment for 4h, however, the vase life was not significantly different in both cut flowers. Since the effects of 1-MCP treatment was differently responded by plant species and cultivars, interacted studies of duration and levels of 1-MCP are needed for the pronounced effects of postharvest quality.