• Title/Summary/Keyword: 실험시설

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Availability of Slurry Composting and Biofiltration for Cultivation of Cherry Tomato (방울 토마토 재배 시 퇴비단 여과 액비의 이용가능성)

  • Kim, Eun-Young;Park, Bong-Ju;Oh, Myung-Min
    • Journal of Bio-Environment Control
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    • v.22 no.4
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    • pp.385-391
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    • 2013
  • In this study, the availability of slurry composting and biofiltration (SCB) solution as an alternative for synthetic nutrient solution was determined by monitoring the growth, fruit yield, and fruit quality of cherry tomato (Solanum lycopersicum L. 'Unicon'). Treatments for nutrient solution were consist of SCB 1/2N, 1N, 2N, and commercial nutrient solution 1N (CNS 1N) based on nitrogen concentration (218.32 $mg{\cdot}L^{-1}$) of cherry tomato nutrient solution (control 1N). All nutrient solution including SCB solution (440~520 mL per day) was supplied to rock wool medium using a timer. After 31 days of transplanting, fresh and dry weights of shoots, leaf area, plant height, stem diameter, SPAD value and number of node were measured. After measuring growth characteristics of tomato plants, total fruit yield, ratio of marketable fruit yield, fruit weight, total soluble solids content, total acidity, total phenolic concentration, and antioxidant capacity were determined once a week for 7 weeks. As a result, among the SCB treatments, SCB 1/2N was similar to control 1N and CNS 1N in terms of fresh and dry weights of shoots, leaf area, stem diameter, number of node, and SPAD value. Increased N concentration of SCB inhibited the growth of tomato plants. Total fruit yield of SCB 1/2N was 47% of that of control 1N which showed the best result. Percentage of marketable fruit yield in SCB 1/2N was about 58%. Soluble solids contents, total acidity, total phenolic concentration and antioxidant capacity was the highest in SCB 2N and the other treatments were not shown any difference. Blossom-end rot rarely occurred in control 1N and CNS 1N while SCB treatments without Ca induced the physiological disorder of 7~19%. In conclusion, SCB 1/2N was good for the vegetative growth of cherry tomato plants but reduced yield and quality of fruit compared with control 1N and CNS 1N. Thus, it is possible to apply SCB solution to grow cherry tomato plants hydroponically but in the consideration of fruits yield and quality additional supply of several minerals would be required.

Biological Control of Tobacco Cutworm, Spodoptera litura (Lepidoptera: Noctuidae) by Steinernematid and Heterorhabditid Entomopathogenic Nematodes (Steinernematid와 Heterorhabditid 곤충병원성 선충을 이용한 담배거세미나방 (Spodoptera litura)의 생물적 방제)

  • Kim, Hyeong-Hwan;Cho, Sung-Rae;Choo, Ho-Yul;Lee, Sang-Myeong;Jeon, Heung-Yong;Lee, Dong-Woon
    • Korean journal of applied entomology
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    • v.47 no.4
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    • pp.447-456
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    • 2008
  • Five effective strains against tobacco cutworm, Spodoptera litura (Lepidoptera: Noctuidae), Steinernema carpocapsae (GSN1), Steinernema sp. (GSNUS-10), Steinernema sp. (GSNUS-14), Heterorhabditis bacteriophora Hamyang (HbH), and Heterorhabditis sp. (GSNUH-1) were selected among 14 isolates of Korean entomopathogenic nematode in laboratory tests. $LC_{50}$ values of above five strains against tobacco cutworm were various by different nematode strains and developmental stages of tobacco cutworm. $LC_{50}$ value of S. carpocapsae (GSN1) was the lowest by $4.0{\sim}8.3$ infective juveniles (Ijs) and 2nd instars of tobacco cutworm was most susceptible. Pathogenicity of five effective strains against tobacco cutworm depends on nematode strain, concentration, and application times. The most effective strain was determined as S. carpocapsae (GSN1). Two or three times of applications were effective regardless of nematode strain, or concentration. Efficacy of S. carpocapsae (GSN1), Steinernema (GSNUS-10), Steinernema (GSNUS-14), and Heterorhabditis (GSNUH-1) was variable depending on nematode strain, concentration, application times, and host variety. S. carpocapsae (GSN1) was the most effective and inoculation of 100,000 infective juveniles per m2 (720,000 Ijs/7.2 $m^2=1{\times}10^9$ Ijs/ha) resulted in higher efficacy. Three times of application of nematodes led to higher control efficacy than one or two applications. Efficacy of nematodes was higher on Chinese cabbage than cabbage or kale.

Bionomics of Tetranychus urticae Koch on Eggplants under Various Nitrogen Regimes in Controlled Environment (시설 재배 가지에서 질소 시비 수준에 따른 점박이응애의 생물적 특성)

  • Kim, Ju;Lee, Sang-Koo;Kim, Jeong-Man;Kim, Tae-Heung;Moon, Hyung-Cheol;Choi, Kyu-Hwan;Choi, Dong-Chil
    • Korean journal of applied entomology
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    • v.47 no.3
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    • pp.237-248
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    • 2008
  • Development of T. urticae was studied on the leaves of eggplant grown in hydroponics with nitrogen contents of 5 mM, 10 mM, 30 mM, and 60 mM. As the nitrogen level in hydroponics increased, it also increased in the plant whereas that of K, Ca, and Mg decreased. More nitrogen in hydroponics resulted in increased contents of water and crude protein, and decreased ash, carbohydrates, and fibers within the plant. Biomass was the heaviest as 989.5 g at 10 mM and the lightest at 60 mM. Leaf thickness and the content of chlorophyll increased as the content of nitrogen increased. Laboratory leaf disc tests obtained from plants grown at various nitrogen levels revealed that feeding and oviposition preferences of T. urticae were high at 30 mM and low at 5 mM. Ratio of damaged leaf by naturally occurring T. urticae on eggplants of 99 days post-transplant in the greenhouse was the highest as 98% at 60 mM. Degrees of damage on eggplants with and without T. urticae infestation turned up more as the differences in the levels of nitrogen in the hydroponics get bigger. No definite differences in the rate of T. urticae development was found between nitrogen treatment levels but, mortalities in immature stages dropped as the nitrogen levels went up. Adult longevity was the longest of 11.9 for female and 6.9 days for male at 60 mM. Oviposition period was also the longest as 11.7 days at 60 mM and shortened as the level of nitrogen decreased. The number of eggs oviposited was the most as 144.4 at 60 mM while it was the least as 41.0 at 5 mM. Sex ratio was 0.75 in favor of female at 10 mM. $R_o$ and T increased, no trends were detected in $r_m\;and\;{\lambda}$, while Dt decreased as the levels of nitrogen went up.

Inactivation of Pathogenic Bacteria by Addition of Thermophilic Bacteria in the Thermophilic Aerobic Oxidation(TAO) System (고온호기산화장치의 고온미생물 첨가에 의한 병원성 미생물의 불활성화)

  • Lee W. I.;H. Tsujii;T. Maki;Lee M. G.
    • Journal of Animal Environmental Science
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    • v.10 no.2
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    • pp.111-118
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    • 2004
  • This study analyzed temperature increase, microorganism changes, and inactivation of pathogenic microorganisms in pig slurry when treated with thermophilic microorganisms in Thermophilic Aerobic Oxidation(TAO) system. An amount of $6 m^3$ of pig slurry was treated in an $18 m^3(3.0\times2.5\times2.4 m)$ reactor for 5 to 7 days in two groups: the control of pig slurry only and the treatment of pig slurry with 6 liters of thermophilic microorganism(Bacillus sp.). To study the microorganism changes in the reactor, the populations of aerobic mesophilic microorganisms, thermophilic microorganisms and general pathogens were analyzed. To study the inactivation of pathogenic microorganisms, the levels of E. coli, Salmonella sp, Crytosporidium parvum and Giardia lamblia were analyzed. The temperature inside the reactor ranged from 18 to $62^{\circ}C$ for the control while far the treatment group it ranged from 18 to $66^{\circ}C$, showing a slightly higher array. With regard to changes in microorganisms, both mesophilic and thermophilic organisms decreased from $3.1\times10^6$ to $1.2\times10^2$ CFU/ml and from $1.0\times10^4$ to $8.0\times10^1$ CFU/ml, respectively, in the control. In the treatment, on the other hand, mesophilic organisms decreased from $3.0\times10^8$ CFU/ml to $8.6\times10^5$ CFU/ml while thermophilic organisms increased sharply from $2.0\times10^6$ to $1.2\times10^8$ CFU/ml. For pathogens, Salmonella and Giardia were not detected either before or after the treatment, while E. coli and C. parvum were found to be $10^5$ CFU/ml each before treatment and negative after it. From this experiment, it was concluded that thermophilic microorganisms could effectively sanitize liquid compost by generating high temperature in the TAO system, which in turn would inhibit the growth of pathogenic organisms.

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Effects of Ventilation Systems on Interior Environment of the Growing-finishing Pig House in Korea (육성$\cdot$비육돈사 내에서 환기형태별 환경조사 연구)

  • Song J. I.;Yoo Y. H.;Jeong J. W.;Kim T. I.;Choi H. C.;Kang H. S.;Yang C. B.;Lee Y. Y.
    • Journal of Animal Environmental Science
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    • v.10 no.2
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    • pp.93-100
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    • 2004
  • An experiment was conducted to establish comparison of ventilation efficiency in an enclosed and conventional growing-finishing pig house. The experimental pigs were in winter and summer. The main results of the experiment are as follows : Then the air from planar slot inlet the pig house flow out through the sidewall outlet operated by exhaust fan(Gl). The second structure has an air input through the circular duct inlet are plated side the juncture of the entering wall and the air into the pig house flow out through the chimney and pit outlet are operated by exhaust fan(G2). Through the air into relay fan the pig house flow out through the curtains in sidewall(G3). Similarly, air comes in through the circular duct inlet are placed the air into the pig house flow out through the curtains in sidewall (G4). Air flow rate on the floor level which is the low part of pen and the living area of pigs in the G2 and G4 system during winter was measured at 0.2 to 0.3 m/s at the 0.5 to 0.6 m/s at the maximum ventilation efficiency. As for the results of detrimental gas(ammonia) concentration ratio analysis, while G2 and G4 system sustained of summer 13.3 $\~$ 16.6 ppm, winter 14.0 $\~$ 14.6 ppm level, Gl and G3 system sustained of summer 14.6 $\~$ 20.3 ppm, winter 20.3 $\~$ 25.0 ppm, and the latter one is lower than that of the G1 and G3 system.

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Basic Study on the Development of Analytical Instrument for Liquid Pig Manure Component Using Near Infra-Red Spectroscopy (근적외선 분광법을 이용한 돈분뇨 액비 성분분석기 개발을 위한 기초 연구)

  • Choi, D.Y.;Kwag, J.H.;Park, C.H.;Jeong, K.H.;Kim, J.H.;Song, J.I.;Yoo, Y.H.;Chung, M.S.;Yang, C.B.
    • Journal of Animal Environmental Science
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    • v.13 no.2
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    • pp.113-120
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    • 2007
  • This study was conducted to measure Nitrogen(N), Phosphate($P_2O_5$), Potassium ($K_2O$), Organic matter(OM) and Moisture content of liquid pig manure by Near Infrared Spectroscopy(NIRS) and to develop an alternative and analytical instrument which are used for measurement of N, $P_2O_5$, $K_2O$, OM, and Moisture contents for liquid pig manure. The liquid pig manure sample's transmittance spectra were measured with a NIRS in the wavelength range of 400 to 2,500 nm. Multiple linear regression and partial least square regression were used for calibrations. The correlation coefficient(RSQ) and standard error of calibration(SEC) obtained for nitrogen were 0.9190 and 2.1649, respectively. The RSQ for phosphate, potassium, organic matter and moisture contents were 0.9749, 0.5046, 0.9883 and 0.9777, and the SEC were 0.5019, 1.9252, 0.1180 and 0.0789, respectively. These results are indications of the rapid determination of components of liquid pig manure through the NIR analysis. The simple analytical instrument for liquid pig manure consisted of a tungsten halogen lamp for light source, a sample holder, a quartz cell, a SM 301 spectrometer for spectrum analyzer, a power supply, an electronics, a computer and a software. Results showed that the simple analytical instrument that was developed can approximately predict the phosphate, organic matter and moisture content of the liquid pig manure when compared to the analysis taken by NIRS. The low predictability value of potassium however, needs further investigation. Generally, the experiment proved that the simple analytical instrument was reliable, feasible and practical for analyzing liquid pig manure.

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Effects of Environment Friendly Agricultural Materials to Phytoseiulusc persimilis (Acari: Phytoseiidae) in the Laboratory (실내조건에서 친환경농자재가 포식성 칠레이리응애, Phytoseiulus persimilis(Acari: Phytoseiidae)에 미치는 영향)

  • Kang, Myong-Ki;Kang, Eun-Jin;Lee, Hee-Jin;Lee, Dae-Hong;Seok, Hee-Bong;Kim, Da-A;Gil, Mi-La;Seok, Mi-Ja;Yu, Yong-Man;Youn, Young-Nam
    • Korean journal of applied entomology
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    • v.46 no.1 s.145
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    • pp.87-95
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    • 2007
  • Many kinds of environment friendly agricultural materials were used for the insect pest control and the control of plant diseases, furthermore they support the growth of crops in the greenhouses and the kindly environment friendly farming. Phytoseiulus persimilis might be used for control of two-spotted mites with environment friendly agricultural materials at the same time. For testing the toxicity of environment friendly agricultural materials against to p. persimilis, 61 environment friendly agricultural materials were selected by material contents and using methods. When environment friendly agricultural materials were directly sprayed on P. persimilis, IEFAM C, FEFAM A, EFAMSM A, D, EFAMPE A, EFAMCh B, EFAMME A, and EFAMMo C killed over 90%. However, there was no effects to FEFAM C, D, EFAMSM C, EFAML A, EFAMME C, E, H, J, EFAMMo G and I against P. persimilis. P. persimilis adults were not survived in vial for 48 hours after sprayed and dried with the environment friendly agricultural materials, fer examples, EFAMSM I, EFAMME A, EFAMMo A, C, and I. Otherwise, EFAMCh C and EFAMMo B were no effects to P. persimilis. Some environment friendly agricultural materials are of different qualities, and consequently test of their foxily have to necessary.

Performance Test of Portable Hand-Held HPGe Detector Prototype for Safeguard Inspection (안전조치 사찰을 위한 휴대형 HPGe 검출기 시제품 성능평가 실험)

  • Kwak, Sung-Woo;Ahn, Gil Hoon;Park, Iljin;Ham, Young Soo;Dreyer, Jonathan
    • Journal of Radiation Protection and Research
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    • v.39 no.1
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    • pp.54-60
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    • 2014
  • IAEA has employed various types of radiation detectors - HPGe, NaI, CZT - for accountancy of nuclear material. Among them, HPGe has been mainly used in verification activities required for high accuracy. Due to its essential cooling component(a liquid-nitrogen cooling or a mechanical cooling system), it is large and heavy and needs long cooling time before use. New hand-held portable HPGe has been developed to address such problems. This paper deals with results of performance evaluation test of the new hand-held portable HPGe prototype which was used during IAEA's inspection activities. Radioactive spectra obtained with the new portable HPGe showed different characteristics depending on types and enrichments of nuclear materials inspected. Also, Gamma-rays from daughter radioisotopes in the decay series of $^{235}U$ and $^{238}U$ and characteristic x-rays from uranium were able to be remarkably separated from other peaks in the spectra. A relative error of enrichment measured by the new portable HPGe was in the range of 9 to 27%. The enrichment measurement results didn't meet partially requirement of IAEA because of a small size of a radiation sensing material. This problem might be solved through a further study. This paper discusses how to determine enrichment of nuclear material as well as how to apply the new hand-held portable HPGe to safeguard inspection. There have been few papers to deal with IAEA inspection activity in Korea to verify accountancy of nuclear material in national nuclear facilities. This paper would contribute to analyzing results of safeguards inspection. Also, it is expected that things discussed about further improvement of a radiation detector would make contribution to development of a radiation detector in the related field.

Selection of Filamentous Cyanobacteria and Optimization of Culture Condition for Recycling Waste Nutrient Solution (폐양액 활용을 위한 Filamentous Cyanobacteria의 선발 및 최적배양)

  • Yang, Jin-Chul;Chung, Hee-Kyung;Lee, Hyoung-Seok;Choi, Seung-Ju;Yun, Sang-Soon;Ahn, Ki-Sup;Sa, Tong-Min
    • Korean Journal of Soil Science and Fertilizer
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    • v.37 no.3
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    • pp.177-183
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    • 2004
  • The discharge of waste nutrient solution from greenhouse to natural ecosystem leads to the accumulation of excess nutrients that results in contamination or eutrophication. There is a need to recycle the waste nutrient solution in order to prevent the environmental hazards. The amount and kind of nutrients in waste nutrient solution might be enough to grow photosynthetic microorganisms. Hence in the present study, we examined the growth and mass cultivation of cyanobacteria in the waste nutrient solution with an objective of removing N and P and concomitantly, its mass cultivation. Four photosynthetic filamentous cyanobacteria (Anabaena HA101, HA701 and Nostoc HN601, HN701) isolated from composts and soils of the Chungnam province were used as culture strains. Among the isolates, Nostoc HN601 performed faster growth rate and higher N and P uptake in the BG-II ($NO_3{^-}$) medium when compared to those of other cyanobacterial strains. Finally, the selected isolate was tested under optimum conditions (airflow at the rate of $1L\;min^{-1}$. in 15 L reactor, initial pH 8) in waste nutrient solution from tomato hydroponic in green house condition. Results showed to remove 100% phosphate from the waste nutrient solution in the tomato hydroponics recorded over a period of 7 days. The growth rate of Nostoc HN601 was $16mg\;Chl-a\;L^{-1}$ in the waste nutrient solution from tomato hydroponics with optimum condition, whereas growth rate of Nostoc HN601 was only $9.8mg\;Chl-a\;L^{-1}$ in BG-11 media. Nitrogen fixing capacity of Nostoc HN601 was $20.9nmol\;C_2H_4\;mg^{-1}\;Chl-a\;h^{-1}$ in N-free BG-11. The total nitrogen and total phosphate concentration of Nostoc HN601 were 63.3 mg N gram dry weight $(GDW)^{-1}$ and $19.1mg\;P\;GDW^{-1}$ respectively. Collectively, cyanobacterial mass production using waste nutrient solution under green house condition might be suitable for recycling and cleaning of waste nutrient solution from hydroponic culture system. Biomass of cyanobacteria, cultivated in waste nutrient solution, could be used as biofertilizer.

Ameliorating Effect of $\textrm{Ca}({NO_3})_2$ or $\textrm{CaCl}_2$ on the Growth and Yield of NaCl-Stressed Tomato Grown in Plastic Pots Filled with Soil (NaCl 스트레스를 받은 토마토의 생육 향상을 위한 $\textrm{Ca}({NO_3})_2$$\textrm{CaCl}_2$ 처리 효과)

  • 강경희;권기범;최영하;김회태;이한철
    • Journal of Bio-Environment Control
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    • v.11 no.3
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    • pp.127-132
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    • 2002
  • Enhanced supply of $Ca^{2+}$ as well as NO$_3$$^{[-10]}$ is known to restrict the uptake of the Na$^{+}$ and Cl$^{[-10]}$ ion and ameliorate growth under saline conditions. This test was conducted to investigate the ameliorating effects of Ca(NO$_3$)$_2$ or CaCl$_2$ on the growth and yield of NaCl-stressed tomato plants grown in plastic pot filled with soil. All treatments except for the control were supplied with 80 mM NaCl fur two weeks after transporting. The saline solutions with nutrient were supplemented with either 0, 10 or 20 mM Ca(NO$_3$)$_2$ and either 0, 10 or 20 mM CaCl$_2$ during harvesting time from two weeks after transporting. Ca(NO$_3$)$_2$ or CaCl$_2$ application enhanced the growth such as plant height, fresh weight, dry weight, fruit number, and fruit weight, and yield of NaCl-stressed tomato, and also their effects increased greater as concentration of supplemented Ca(NO$_3$)$_2$ or CaCl$_2$increased. Yield increased in 20 mM Ca(NO$_3$)$_2$ compared with the others except fur the control. Photosynthetic rate in Ca treatments was lower than that of the control, but higher than that of NaCl treatment. Leaf chlorophyll content was higher in Ca treatments compared with the others, especially in younger leaf, while that was not affected by concentration of supplemented Ca. Ca(NO$_3$)$_2$ or CaCl$_2$ supply increased the $K^{+}$ and $C^{2+}$ concentration of tomato plants, whereas the Na$^{+}$ transport to the leaves was inhibited. There was a strong increase in the $K^{+}$/Na$^{+}$ ratio in plants treated Ca(NO$_3$)$_2$, or CaCl$_2$. Cl$^{[-10]}$ content of plants was decreased by supplemental Ca(NO$_3$)$_2$ but Cl$^{[-10]}$ was increased in plants with CaCl$_2$compared with Ca(NO$_3$)$_2$. N concentration in plants of tomato increased with enhanced Ca(NO$_3$)$_2$ or CaCl$_2$supply, In conclusion, our study confirms the potential of Ca(NO$_3$)$_2$ or CaCl$_2$to alleviate NaCl-induced growth reductions in tomato.