• KSBB Journal
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• v.11 no.3
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• pp.270-275
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• 1996

Heterologous expression of glucose oxidase gene using recombinant yeast has been carried out. Polymerase chain reaction was conducted to obtain the gene encoding glucose oxidase from Aspergillus niger and sequence comparison indicated the cloned 1.9kb DNA fragment appeared to be the glucose oxidise structural gene containing a signal sequence for extracellular location. Transforming shuttle vector was constructed with YEp352 to express the cloned glucose oxidase gene under the control of either GAL1 or GAL10 promoter. Plate assay of recombinant yeasts has shown that GAL1 promoter was more effective in yielding glucose oxidise than GAL10 promoter. Among the five different concentrations of galactose tried, 1% galactose showed the highest induction of glucose oxidase. Cellular localization experiment of recombinant enzyme using spheroplast revealed that most of enzymes (80%) were secreted into culture media in contrast to A. niger. There is no difference in heat-stability of recombinant enzyme up to $50^{\circ}C$ compared to the glucose oxidase from A. niger However, a dramatic reduction of enzyme activity was observed in both enzymes at $60^{\circ}C$.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.21 no.1
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• pp.8-13
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• 2010

In this paper, a new hairpin type strip-line BPF(Band Pass Filter) improving the suppression performance of unwanted frequency signal is studied. A modified hairpin filter is designed by classical hairpin filter design methodology and is realized by newly placing structure of designed filter. And a newly formed coupled-line effects between modified bilateral symmetry structures make the transmission zeros. Each transmission zeros can shift its frequency to wanted frequency by tuning a certain part of filter. To investigate the validity of this novel technique, an order-5 Chebyshev BPF centered at 9.2 GHz with a 15 % FBW(fractional bandwidth) were used. According to design and measurement results, a good performance of insertion loss of 0.8 dB and unwanted signal suppression of maximum 50 dB is achieved at full input/output ports.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2000.10a
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• pp.463-467
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• 2000

In this paper, we have implemented the Web Server Patient Monitoring System using PPP. It is composed of two parts. The first part is the Analog board for acquiring ECG signals. The second part is the module for processing and transmitting the acquired signal. The second part is using PPP for dial-up networking, TCP/IP for Internet, HTTP for web browser and JAVA program for a Patient Monitoring Program in one chip. In home, it is not need to establish another network line because it uses a telephone line. And a user who want to monitor a patient's biosignal can monitor a patient without wholly open network because it is the network sewer. The Patient Monitoring Program runs on a web browser by downloaded JAVA codes when a user connect to this system. It can make the Home Patient Monitoring Program decrease cost. It can help to avoid the limitation of monitoring a patient.

• KIISE Transactions on Computing Practices
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• v.20 no.11
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• pp.579-585
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• 2014

A phoneme is the minimal unit of speech, and it plays a very important role in speech recognition. This paper proposes a novel method that can be used to recognize 'ㅅ', 'ㅈ', and 'ㅊ' among Korean phonemes. The proposed method is based on a volatility indicator and a turning point indicator that are calculated for each constituting block of the input speech signal. The volatility indicator is the sum of the differences between the values of each two samples adjacent in a block, and the turning point indicator is the number of extremal points at which the direction of the increment or decrement of the values of the sample are inverted in a block. A phoneme recognition algorithm combines the two indicators to finally determine the positions at which the three target phonemes mentioned above are recognized by utilizing optimized thresholds related with those indicators. The experimental results show that the proposed method can markedly reduce the error rate of the existing methods both in terms of the false reject rate and the false accept rate.

• The Transactions of the Korean Institute of Power Electronics
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• v.5 no.5
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• pp.459-466
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• 2000

A new control method for precision robust speed control of a PMSM(Permanent Magnet Synchronous Motor) using load torque observer is presented. Using this system, we can more precisely evacuate of vestibular function. Until now a rotating chair system, so called 2D-stimulator, which has vertical rotate axis is used to make dizziness. However, an inclined rotating chair system witch is called 3D-stimulator is needed to obtain the precise dizziness data. This 3D-stimulator include unbalanced load caused by unbalanced center of mass. In this case, new compensation method is considered to obtain robust speed control using load torque observer. To reduce the effect of this disturbance, we can use dead-beat observer that has high gain. The application of the load to torque observer is published in for position control. However, there is a problem of using speed information such as amplifying effect of noise. Therefore, we can reduce a noise effect by moving average process. The experimental results are depicted in this paper to show the effect of this proposed algorithm.

• The KIPS Transactions:PartB
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• v.8B no.5
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• pp.579-586
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• 2001

Digital watermarking is to embed imperceptible mark into image, video, audio, and text data to prevent the illegal copy of multimedia data. arbitrary modification, and also illegal sales of the copies without agreement of copyright ownership. In this paper, we study for the embedding and extraction of watermark key using wavelet in the luminance signal in order to implement the system to protect the copyright for image MPEG. First, the original image is analyzed into frequency domain by discrete wavelet transform. The RSA(Rivest, Shamir, Aldeman) public key of the coded target is RUN parameter of VLD(variable length coding). Because the high relationship among the adjacent RUN parameters effect the whole image, it prevents non-authorizer not to possess private key from behaving illegally. The Results show that the proposed method provides better moving picture and the distortion more key of insert than direct coded method on low-frequency domain based DCT.

• 한국지구물리탐사학회:학술대회논문집
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• 2009.10a
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• pp.119-123
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• 2009

Gravity changes due to variations of groundwater level were measured at a ground water monitoring well, which is located at Kum-san, Korea, from November 2008 to September 2009 using Portable Earth Tide (PET) gravimeter. Groundwater level was monitored simultaneously with gravity observations. To extract gravity information from groundwater, we reduced gravity effect from atmospheric surface pressure, earth tides and its loading effect, polar motion and meter drift. In addition, in June 4, 2009, there was a pumping test at he observation well, and groundwater level and gravity variations were observed together successfully. Observation of gravity along with groundwater level is potentially useful for monitoring of aquifer water mass balance and water resources.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.48 no.11
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• pp.903-910
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• 2020

This paper introduces a simulator to assess the impacts of the wind and the airframe vibration on the performance of the Active Electronically Scanned Array (AESA) radar mounted in an aircraft. The AESA radar is mounted on the nose cone of an aircraft, and vibration occurs due to the drag force. This vibration affects the behavior of the AESA radar and can cause phase errors in signal. The simulator adopts the geometric model for nose cone, the mathematical models on the rigid-body dynamics of the aircraft, the average/turbulent winds, and the mode/ambient vibrations to compute the position and the attitude of the radar accurately. Numerical studies reflecting a set of test scenarios were conducted to demonstrate the effectiveness of the developed simulator.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.37 no.8A
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• pp.712-719
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• 2012

This paper presents Single-bit Third order Delta-Sigma Modulator, which can be applied to the Low speed High resolution ADC in Audio signal Process System. Whereas the Operational Amplifier in modulator consumed static power dissipation in operating, this modulator used Switching on/off techniques, which makes the Power dissipation of the modulator reduced. Also proposed modulator minimizes frequency characteristic variation by optimizing switch position. And this modulator chooses Single-bit type to guarantee stability. The designed ADC went through 0.35um CMOS n-well 1-poly 4-metal process to be a final product, and the final product has shown 17.1mW of power dissipation with 3.3V of Supply Voltage, 6.4MHz of conversion rate. And 84.3dB SNDR and 13.5bit ENOB with 20KHz of input frequency.

• Journal of the Korean Magnetics Society
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• v.20 no.2
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• pp.45-51
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• 2010

We suggest the algorithm that it detects volume and shape according with a variation of magnetic field in non-contact electromagnetic measurement system. It is possible to assess an object shape through a variation of magnetic field. The basic idea is compared a length difference with a variation of magnetic field in a detected object and a circle which modeled equivalent area. And the shape is detected to many calibration process that it is similar to signal pattern between a length difference and a variation of magnetic field in object and equivalent circle. This is the shape detection algorithm that use only the variation of magnetic field. In this paper, it has application to the shape detection algorithm about the object as hexagon, pentagon, rectangle, trigon. we can detect the object shape easily because the shape detection algorithm is only used to the variation of magnetic field.