• Korean Journal of Environment and Ecology
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• v.37 no.6
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• pp.450-463
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• 2023

The objective of this study is to derive the core habitat of the Kirengeshoma koreana Nakai utilizing Habitat Suitability Index (HSI) and Maximum Entropy (MaxEnt) models. Expert-based models have been criticized for their subjective criteria, while statistical models face difficulties in on-site validation and integration of expert opinions. To address these limitations, both models were employed, and their outcomes were overlaid to derive the core habitat. Five variables were identified through a comprehensive literature review and spatial analysis based on appearance coordinates. The environmental variables encompass vegetation zone, forest type, crown density, annual precipitation, and effective soil depth. Through surveys involving six experts, importance rankings and SI (Suitability Index) scores were established for each variable, subsequently facilitating the creation of an HSI map. Using the same variables, the MaxEnt model was also executed, resulting in a corresponding map, which was merged to construct the definitive core habitat map. Out of 16 observed locations of K. koreana, 15 were situated within the identified core habitat. Furthermore, an area historically known to host K. koreana but not verified in the present, Mt. Yeongchwi, was found to lack a core habitat. These findings suggest that the developed models exhibit a high degree of accuracy and effectively reflect the current ecological landscape.

• Korean Journal of Environment and Ecology
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• v.28 no.1
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• pp.62-70
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• 2014

This study was carried out to analysis the vegetation status, the habitat size and the meaning of new natural habitat, and to provide basic data for conservation or management on the newly augmented natural habitat of Abeliophyllum distichum Nakai in Yeoju-si, Gyunggi-do, Korea. A. distichum is a Korean monotypic endemic species. Most natural habitats of A. distichum have been reported in South-Central area around Chungcheong-do. The extent of occurrence of A. distichum can be enlarged to Central area due to the Yeoju-si habitat. The new habitat is located on a hillock which is composed of rock field at $37^{\circ}20{\sim}21^{\prime}N$, $137^{\circ}43{\sim}21^{\prime}E$, and 99~120m a.s.l.. The habitat size is small as about $530m^2$, and a small stream runs aside. The number of individuals of A. distichum was about 1,200. But most individuals were smaller than 0.5m height, so just about 300 individuals are taller than 1.0m height. A. distichum on Yeoju-si seemed to be maintained and distributed by vegetative propagation from elongated roots of branches. Pinus rigida and Quercus aliena dominated tree layer, and Lindera obtusiloba, Q. aliena and Acer tatricum appeared frequently in subtree layer. The shrub layer was dominated by A. distichum with Stephanandra incisa, Euonymus alatus for. ciliatodentatus, Ligustrum obtusifolium, etc. The coverage of herbaceous layer was low.

• Korean Journal of Weed Science
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• v.15 no.1
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• pp.73-84
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• 1995

Four malformin A's produced by Aspergillus niger van Tiegh. were separated by HPLC equipped with $C_{18}$ reversed-phase column and subjected to structural determination. Amino acid analyses and mass spectra data of the compounds indicate that they structurally resemble the cyclic pentapeptide malformin $A_1$. Their structures were deduced by two dimensional NMR and MS/MS experiments as cyclo-D-Cys-D-Cys-L-Val-D-Leu-L-Ile for $A_1$, cyclo-D-Cys-D-Cys-L-Val-D-Leu-L-Val for $A_2$, cyclo-D-Cys-D-Cys-L-Val-D-Leu-L-Leu for $A_3$, and cyclo-D-Cys-D-Cys-L-Val-D-Ile-L-Val for $A_4$. Among the mal-formin A's, the structure of $A_3$ was identical to that of malformin C, which was produced by A. niger strain AN-1. All the malformin A's caused severe curvatures of corn(Zea mays L.) roots and the activities of the malformin A's with molecular weight 529 were greater than those with molecular weight 515. Malformin $A_1$ caused the corn root curvature by 83% at a concentration of $0.25{\mu}M$. In the mung bean(Phaseolus aureus Roxb.) hypercotyl segment test, however, the molecular weight of malformin A's was not a factor influencing the physiological activities. Malformin $A_1$ stimulated the growth of mung bean hypercotyles by 165% at a $0.1{\mu}M$ concentration.

• Korean Journal of Environmental Biology
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• v.24 no.1 s.61
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• pp.38-45
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• 2006

Ecological characteristics of microbial populations inhabiting heavy metal polluted soil were investigated. The samples were collected from 293 sites around an factory and industry at Gyeoungsangbuk-do. We measured the contents of seven heavy metal elements (Cd, Cu, As, Hg, Pb, $Cr^{6+}$, CN), seven sites have been seriously contaminated by mercury and chrome. A quantitative evaluation of microbial populations in mercury and chrome contaminated soil was examined by using plate count method. Bacterial numbers in polluted soil samples ranged from $7.4X10^5\;to\;9.3X10^7\;cfu\;g^{-1}$, about $10\sim100$ fold less than the count for the unpolluted soil. Moulds were not detected in chrome polluted soil. The log values of actinomycetes of each contaminated soil samples were log ranged from 6.18 to 7.52. The ratio of actinomycetes was similar to unpolluted soil. The investigation showed actinomycetes to be the major microbial population inhabiting the mercury and chrome polluted soil. Thirty-one isolates among the total isolates were examined for antibacterial activity. These isolates were identified based on a phylogenetic analysis using 16S rRNA gene nucleotide sequences, they were categorized in three major phylogenetic groups, belong to the Streptomyces (6 strains), Saccharopolyspora (3 strains), Nocardiodes (1 strain). On the phylogenetic tree, the clade consisting of five isolates were distantly related to all of the established Streptomycetes genera, indicating the possibility as members of new species.

• Journal of Life Science
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• v.20 no.8
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• pp.1268-1275
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• 2010

This study was carried out to investigate the anti-proliferative activity of solid-state fermented medicinal herbs which include Phellinus baumii. Methanol extracts were prepared from 36 different medicinal herbs and their fermented counterparts. These extracts were used to treat human colorectal HCT116 cell, human embryonic kidney cell HEK-293, pre-adipocyte cell 3T3-L1, and pre-osteoblast cell MC3T3-E1 for 24 hr. At a concentration of 100 ${\mu}g/ml$, the extracts of Amomum villosum, Cnidium officinale Makino, Dendrobium moniliforme, Dictamnus dasycarpus, Diospyros kaki Thunb, Eucommia ulmoides Oliv, Ginkgo biloba L, Magnolia denudata Desrousseaux, Orostachys japonicus, Panax notoginseng, Pharbitis nil Choisy, Polygala tenuifolia and Trichosanthes kirilowii (seed) led to a < 50% decrease in cell proliferation, and mycelium of P. baumii showed a 46.3% decrease in cell proliferation. Meanwhile, the extracts of the 25 fermented herbs showed similar anti-proliferative activities compared to those of individual non-fermented herbs. However, the extracts of the fermented Drynaria fortunei Kunze (1), Lycium chinense Mill (2), Fritillaria thunbergii Miquel (3) and Prunus persica showed increased anti-proliferative activity. The $IC_{50}s$ of (1), (2) and (3) were especially decreased to 28, 85 and 80 ${\mu}g/ml$ from 394, 917 and 149 ${\mu}g/ml$, respectively. Furthermore, the cytotoxicity of the extracts of fermented (1), (2) and (3) against HEK-293, 3T3-L1, and MC3T3-E1was negligible up to 200 ${\mu}g/ml$. These results suggest that solid-state fermentation using the mycellium of P. baumiiproduce potential anti-cancer agents or strengthen the bioactivity of medicinal herbs.

• Journal of Life Science
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• v.30 no.3
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• pp.298-303
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• 2020

This study aimed to investigate possible applications of Rodgersia podophylla in the food and cosmetic industry. Ethanol extracts of leaves (RP-L), branches (RP-B), and root (RP-R) were prepared, and their antimicrobial, antioxidant, and anti-diabetic activities were evaluated. The polyphenol content in the RP-R, RP-L, and RP-B extracts was 79.6, 30.4, and 16.9 mg/g, respectively. An antimicrobial activity assay showed that the RP-L and RP-R extracts exhibited strong growth inhibition of pathogenic and food spoilage Gram-positive bacteria. Furthermore, the RP-R extract inhibited the growth of the Gramnegative E. coli and P. vulgaris bacteria. All extracts showed strong scavenging activity for DPPH, ABTS, nitrite, and reducing power determined by A 700 nm. In particular, the RC₅₀s of the RP-R extract for the DPPH anion and ABTS cation were 23.0-29.7 and 15.0-18.2 ㎍/ml, respectively, which are comparable to those of vitamin C (9.8 and 8.0 ㎍/ml, respectively). An activity assay of α-glucosidase and β-amylase suggested a high potential for the RP-R extract as an anti-diabetic agent. Its inhibition levels of α-glucosidase and β-amylase at 0.5 mg/ml were 6.9 and 48.5%, respectively. This is the first report of the antimicrobial and anti-diabetic activities of R. podophylla. Our results suggest that RP-L and RP-R extracts could be developed as novel cosmeceutical and functional food resources.

• The Korean Journal of Nuclear Medicine
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• v.35 no.5
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• pp.334-341
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• 2001

Purpose: Ascorbic acid us known to act as an antioxidant. Therefore, it can be used in increasing the efficiency of radiochemical labeling of Technetium-99m setamibi by inhibition of oxidation of $Sn^{2+}$ at low concentration. We intended to estimate the efficiency of radiochemical labeling and the stability of the newly formed formulation when ascorbic acid was added to a commercial kit. Materials and Methods: Synthesis of sestamibi was performed according to Dong-A's patent procedure (No.10-2001-0012877). First, we undertook a study to evaluate the efficiency of radiochemical labeling of sestamibi containing ascorbic acid. The stability of the vials was assessed using either $7.5{\mu}g\;or\;75{\mu}g$ of ascorbic acid, added to commercial vials under the accelerated condition(Temp : $40^{\circ}C{\pm}2^{\circ}C$, Relative humidity : $75{\pm}5%$). Results: Sestamibi was synthesized in overall 35-40% yield over 5 steps from a commercially available methallyl chloride as a starling material. When ascorbic acid was added, the efficiency of radiochemical labeling was maintained compared to the vial with no ascorbic acid. The accelerated test showed that the addition of ascorbic acid inhibited the oxidation of $Sn^{2+}$ ion by antioxidation mechanism. Also, the efficiency of radiochemical labeling of this vial after 9 months was nearly the same as the starting point. Therefore, the storage period of the kit is likely to be extended. Taken together, it suggests that the addition of ascorbic acid as a stabilizer is desirable. Conclusion: To increase the stability of a sestamibi cold kit, it is desirable to add ascorbic acid as a stabilizer to the commercial formulation.

• The Journal of the Korea Contents Association
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• v.7 no.4
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• pp.1-9
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• 2007

Cellular operators develop high speed mobile internet and multi-function cellular phones to activate new business model based on mobile internet services. Domestic cellular operators evolve their mobile networks from cdma2000-1x and EvDo to HSDPA to activate high speed mobile internet services. They also develop mobile internet platforms such as WIPI, BREW, and J2ME on multi-function cellular phones having multimedia solutions such as camera, MP3, MPEG, 3D game engine, DMB, PAN such as bluetooth, IrDA, W-LAN, and location information using GPS. But, content providers have problems of redevelopment of the same mobile internet application on different mobile internet platforms provided by cellular operators. In this paper, we develop conversion solutions for interoperability of mobile internet applications on WIPI and BREW using an one-pass compiler. We confirm the performance the proposed conversion solutions for the API conversion rate, the converted file size, and the full conversion time using the popular mobile games which are the killer applications on WiPI and BREW.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.25 no.2
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• pp.363-374
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• 2015

Instrumentation and control(I&C) system has been mainly designed and operated based on analog technologies in existing Nuclear Power Plants(NPPs). However, As the development of Information Technology(IT), digital technologies are gradually being adopted in newly built NPPs. I&C System based on digital technologies has many advantages but it is vulnerable to cyber threat. For this reason, cyber threat adversely affects on safety and reliability of I&C system as well as the entire NPPs. Therefore, the software equipped to NPPs should be developed with cyber security attributes from the initiation phase of software development life cycle. Moreover through cyber security assessment, the degree of confidence concerning cyber security should be measured and if managerial, technical and operational work measures are implemented as intended should be reviewed in order to protect the I&C systems and information. Currently the overall cyber security program, including cyber security assessment, is not established on I&C systems. In this paper, we propose cyber security assessment methods in the Software Development Life Cycle by drawing cyber security activities and assessment items based on regulatory guides and standard technologies concerned with NPPs.

• Food Science of Animal Resources
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• v.31 no.6
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• pp.952-959
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• 2011

A new bacteriocin-producing lactic acid bacteria (LAB) which has been isolated from kimchi was identified as Pediococcus damnosus by use of API kit and 16S rDNA sequencing, and designated as P. damnosus JNU 534. The bacteriocin produced by P. damnosus JNU 534 markedly inhibited the growth of some of LAB and Listeria monocytogenes, whereas other pathogens including Gram negative bacteria were not susceptible. The production of bacteriocin started at the beginning of exponential phase and reached maximum activity at the early stationary phase. The bacteriocin was stable on the wide pH range of 2-9 and heat treatment up to $100^{\circ}C$ for 15 min. The antimicrobial compound was inactivated by treatments of proteolytic enzymes indicating its proteinaceous in nature. The bacteriocin was purified by 30% ammonium sulfate precipitation followed by hydrophobic interaction column and $C_{18}$ column chromatography. The estimated molecular weight of the bacteriocin using tricine SDS-PAGE was approximately 3.4 kDa and the identified N-terminal amino acid sequence was $NH_2$-ILLEELNV.