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http://dx.doi.org/10.5851/kosfa.2011.31.6.952

Characteristics and Partial Purification of a Bacteriocin Produced by Pediococcus damnosus JNU 534  

Lee, Jae-Won (Division of Animal Science, Chonnam National University)
Han, Su-Min (Division of Animal Science, Chonnam National University)
Yun, Bo-Hyun (Division of Animal Science, Chonnam National University)
Oh, Se-Jong (Division of Animal Science, Chonnam National University)
Publication Information
Food Science of Animal Resources / v.31, no.6, 2011 , pp. 952-959 More about this Journal
Abstract
A new bacteriocin-producing lactic acid bacteria (LAB) which has been isolated from kimchi was identified as Pediococcus damnosus by use of API kit and 16S rDNA sequencing, and designated as P. damnosus JNU 534. The bacteriocin produced by P. damnosus JNU 534 markedly inhibited the growth of some of LAB and Listeria monocytogenes, whereas other pathogens including Gram negative bacteria were not susceptible. The production of bacteriocin started at the beginning of exponential phase and reached maximum activity at the early stationary phase. The bacteriocin was stable on the wide pH range of 2-9 and heat treatment up to $100^{\circ}C$ for 15 min. The antimicrobial compound was inactivated by treatments of proteolytic enzymes indicating its proteinaceous in nature. The bacteriocin was purified by 30% ammonium sulfate precipitation followed by hydrophobic interaction column and $C_{18}$ column chromatography. The estimated molecular weight of the bacteriocin using tricine SDS-PAGE was approximately 3.4 kDa and the identified N-terminal amino acid sequence was $NH_2$-ILLEELNV.
Keywords
bacteriocin; Pedidococcus damnosus JNU 534; purification;
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1 Liu, W. and Hansen, J. N. (1990) Some chemical and physical properties of nisin, a small-protein antibiotic produced by Lactococcus lactis. Appl. Environ. Microbiol. 56, 2551-2558.
2 Moon, G. S., Kim, W. J., and Kim, M. H. (2002) Synergistic effects of bacteriocin-producing Pediococcus acidilactici K10 and organic acids on inhibiting Escherichia coli O157:H7 and applications in ground beef. J. Microbiol. Biotechnol. 12, 936-942.   과학기술학회마을
3 Nes, I. F. and Johnsborg, O. (2004) Exploration of antimicrobial potential in LAB by genomics. Curr. Opin. Biotechnol. 15, 100-104.   DOI   ScienceOn
4 Oh, S. (2001) Characteristics of class II bacteriocins produced by lactic acid bacteria. J. Korean Dairy Technol. Sci. 19, 133-146.
5 Oh, S., Kim, M. H., Churey, J. J., and Worobo, R. W. (2003) Purification and Characterization of an antilisterial bacteriocin produced by Leuconostoc sp. W65. J. Microbiol. Biotechnol. 13, 680-686.
6 Oh, S., Roh, H., Ko, H. J., Kim, S., Kim, K. H., Lee, S. E., Chang, I. S., Kim, S. H., and Choi, I. G. (2011) Complete genome sequencing of Lactobacillus acidophilus 30SC isolated from swine intestine. J. Bacteriol. 10, 1128-1133
7 Ray, B. (1992) Nisin of Lactococcus lactis ssp. lactis as a food biopreservative. In: Food biopreservatives of microbial origin. Ray, B. and Daeschel, M. (eds) CRC Press, Boca Raton, FL, pp. 207.
8 Rheem, S., Oh, S., Han, K. S., Imm, J. Y., and Kim, S. H. (2002) New response surface approach to optimize medium composition for production of bacteriocin by Lactobacillus acidophilus ATCC 4356. J. Microbiol. Biotechnol. 12, 449-496.   과학기술학회마을
9 Gonzalez, B., Arca, P., Mayo, B., and Suarez, J. E. (1994) Detection, purification and partial characterization of plantaricin C, a bacteriocin produced by Lactobacillus plantarum strain of dairy origin. Appl. Environ. Microbiol. 60, 2158-2163.
10 Green, G., Dicks, L. M. T., Bruggeman, G., Vandamme, E. J., and Chikindas, M. L. (1997) Pediocin PD-1, a bactericidal antimicrobial peptide from Pediococcus damnosus NCFB 1832. J. Appl. Microbiol. 83, 127-132.   DOI   ScienceOn
11 Hoover, D. G. and Harlander, S. K. (1993) Screening methods for detecting bacteriocin activity. In: Bacteriocins of lactic acid bacteria. Hoover, D. G. and Steenson, L. R. (eds) Academic Press, NY, pp. 23-39.
12 Jack, R. W., Tagg, J. R., and Ray. B. (1995) Bacteriocins of Gram-positive bacteria. Microbiol. Rev. 59, 171-200.
13 Kawai, Y., Sato, T., Toba, T., Samant, S. K., and Itoh, T. (1994) Isolation and characterization of a highly hydrophobic new bacteriocin (Gassericin A) from Lactobacillus gasseri LA39. Biosci. Biotech. Biochem. 58, 1218-1221.   DOI   ScienceOn
14 Kim, D. S. (2002) Characteristics of the bacteriocin from Lactobacillus sp. Oh-B3. Korean J. Microbiol. Biotechnol. 30, 181-188.   과학기술학회마을
15 Kim, W. J. (1993) Bacteriocins of lactic acid bacteria: Their potentials as food biopreservative. Food Rev. Int. 9, 299-313.   DOI   ScienceOn
16 Klaenhammer, T. R. (1993) Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol. Rev. 12, 39-85.   DOI
17 Kwon, D. Y. (1992) Synthesis of stereoisomeric trifluoroethylmandelates and their stereospecificity for the uses as the substrate of lipases in organic solvent. J. Korean Chem. Soc. 36, 125-129.   과학기술학회마을
18 Bhugaloo-Vial, P., Dousset, X., Metivier, A., Sorokine, O., Anglade, P., Boyaval, P., and Marion, D. (1996) Purification and amino acid sequences of piscicocins V1a and V1b, two class IIa bacteriocins secreted by Carnobacterium piscicola V1 that display significantly different levels of specific inhibitory activity. Appl. Environ. Microbiol. 62, 4410-4416.
19 Schagger, H. and von Jagow, G. (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of protein in the range from 1 to 100 kDa. Anal. Biochem. 166, 368-379.   DOI   ScienceOn
20 Schved, F., Lalazar, Y., Henis, Y., and Juven, B. J. (1993) Purification, partial characterization and plasmid-linkage of pediocin SJ-1, a bacteriocin produced by Pediococcus acidilactici. J. Appl. Bacteriol. 74, 67-77.   DOI
21 Bhunia, A. K., Johnson, M. C., and Ray, B. (1987) Direct detection and antimicrobial of Pediococcus acidilatici in sodium dodecyl sulfate-polyacryamide gel electrophoresis. J. Ind. Microbiol. Biotchnol. 2, 319-322.   DOI
22 Bhunia, A. K., Johnson, M. C., Ray, B., and Kalchayanand, N. (1991) Mode of action of pediocin AcH from Pediococcus acidilactici H on sensitive bacterial strains. J. Appl. Bacteriol. 70, 25-33.   DOI
23 Candiano, G., Bruschi, M., Musante, L., Santucci, L., Ghiggeri, G. M., Carnemolla, B., Orecchia, P., Zardi, L., and Righetti, P. G. (2004) Blue silver: A very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis 25, 1327-1333.   DOI   ScienceOn
24 Elegado, F. B. and Kwon, D. Y. (1998) Primary structure and conformational studies of pediocin AcM, a bacteriocin from Pediococcus acidilactici M. Phil. J. Biotechnol. 9, 19-26.
25 Elegado, F. B., Kim, J. W., and Kwon, D. Y. (1997) Rapid purification, partial characterization and antimicrobial spectrum of the bacteriocin, pediocin AcM, from Pediococcus acidilactici M. Int. J. Food Microbiol. 37, 1-11.   DOI   ScienceOn
26 Ennahar, S., Sashihara, T., Sonomoto, K., and Ayaaki, I. (2000) Class II a bacteriocins: biosynthesis, structure and activity. FEMS Microbiol. Rev. 24, 85-106.   DOI
27 Ennahar, S., Sonomoto, K., and Ishizaki, A. (1999) Class IIa bacteriocins from lactic acid bacteria: antibacterial activity and food preservation. J. Biosci. Bioeng. 87, 705-716.   DOI   ScienceOn
28 Worobo, R. W., van Belkum, M. J., Sailer, M., Roy, K. L., Vederas, J. C., and Stiles, M. E. (1995) A signal peptide secretion-dependent bacteriocin from Carnobacterium divergens. J. Bacteriol. 177, 3143-3149.
29 Tagg, J. R., Dajani, A. S., and Wannamaker, L. W. (1976) Bacteriocins of gram-positive bacteria. Bacteriol. Rev. 40, 722-756.
30 Toba, T., Samant, S. K., Yoshioka, E., and Itoh, T. (1991) Reutericin 6, a new bactriocin produced by Lactobacillus reuteri LA 6. Lett. Appl. Microbiol. 13, 281-286.   DOI
31 Kwon, D. Y., Koo, M. S., Ryoo, C. R., Kang, C., H., Min, K. H., and Kim, W. J. (2002) Bacteriocin produced by Pediococcus sp. in kimchi and its characteristics. J. Microbiol. Biotechnol. 12, 96-105.   과학기술학회마을