• Title/Summary/Keyword: 식물체 재분화

Search Result 458, Processing Time 0.026 seconds

Plant Regeneration from Leaf Segment Culture of Chrysanthemum zawadskii ssp. coreanum (한라구절초 잎절편 배양에 의한 식물체 재분화)

  • 박영철;김성용;한태완
    • Korean Journal of Plant Resources
    • /
    • v.17 no.1
    • /
    • pp.43-47
    • /
    • 2004
  • The effect of plant growth regulators and gelling agents for plant regeneration from leaf segment of Chrysanthemum zawadskii ssp. coreanum was investigated. NAA was more effective than BA for plant regeneration. MS medium supplemented with NAA 1 mg/L was the most effective in plant regeneration. The effect of agar and gelite as gelling agent was compared. Agar(0.8%) was more effective than gelite(0.2%) in plant regeneration. Regenerated shoots was successfully increased by shoot grafting in MS medium supplemented with NAA 0.1 mg/L in vitro, and hardened by shoot grafting in artificial soil mix(Peatmoss : Perlite = 1 : 1).

Effect of Orchardgrass Varieties on Callus Culture and Plant Regeneration (오차드그래스의 품종이 캘러스배양과 식물체 재분화에 미치는 영향)

  • Lee, Sang-Hoon;Lee, Ki-Won;Lee, Dong-Gi;Kim, Do-Hyun;Lee, Byung-Hyun
    • Journal of The Korean Society of Grassland and Forage Science
    • /
    • v.26 no.4
    • /
    • pp.187-192
    • /
    • 2006
  • In order to investigate the effects of genetic variations of orchardgrass in tissue culture response, calli were induced from mature seeds of eight varieties, 'Hapsung 2', '93E', 'Amba', 'Ambassdor', 'Frode', 'Frontier', 'Potomac' and 'Roughrider', and plant regeneration frequency was compared. Significant differences were observed among the varieties in both callus induction and plant regeneration. Callus induction rate of viable seeds varied from 24.3% to 71.7%. Plant regeneration frequency ranged from 76.6% to 29.7%. 'Roughrider' varieties showed higher regenerability with the frequency of 76.6%. These results can be used not only to provide additional improvements in the plant regeneration frequency from transgenic callus, but also useful for molecular breeding of orchardgrass through genetic transformation.

Rapid Regeneration of Plants on N6 Medium from Orchardgrass (Dactylis Glomerate L.) Calli (N6 배지에서 오차드그래스 캘러스로부터 빠른 재분화)

  • 김기용;임용우;최기준;신재순;김정갑;조진기
    • Journal of The Korean Society of Grassland and Forage Science
    • /
    • v.18 no.3
    • /
    • pp.267-272
    • /
    • 1998
  • We confirmed conditions for callus formation and plant regeneration of five varieties of orchardgrass (Dactylis glomerma L.). Among five varieties of orchardgrass, "Hapsung 19" expressed the highest rate for both of callus formation and plant regeneration. Otherwise, among SH (Schenk and Hildebrandt), MS (Murashige and Skoog) and N6 medium (Chu), MS and N6 medium were highest degree of efficiencies in callus formation and plant regeneration, respectively. In this study, we determined volume of hormones appended in media; $3\;mg/\;{\ell}$ ofdicamba for callus formation and $1\;mg/\;{\ell}$ of NAA (I-naphtalene acetic acid) and $5\;mg/\;{\ell}$ kinetin (6-furfurylaminopurine) for plant regeneration were appended in their media. We obtained orchardgrass plants from callus about 50~80 days after transferred to regeneration media.ation media.

  • PDF

Callus Induction from Seeds of Italian ryegrass and Plant Regeneration (이탈리안 라이그라스 종자로부터 캘러스 유도 및 식물체 재분화)

  • 임용우;김기용;최기준;성병렬;신정섭
    • Journal of The Korean Society of Grassland and Forage Science
    • /
    • v.20 no.1
    • /
    • pp.25-30
    • /
    • 2000
  • The conditions for callus formation and plant regeneration were confirmed in Italian ryegrass (Lolium mulfiorum Lam.). Among SH (Schenk and Hildebrandt), MS (Murashige and Skoog) and N6 medium (Chu) MS medium was highest degree of efficiencies respectively in callus formation and plant regeneration. In this study, we determined volume of hormones and other compounds appended in media. For callus formation, only $5\;mg/\;{\ell}$ of 2,4-D (2,4-dichlorophenoxy acetic acid) was appended in their media. For plant regeneration, we used MS medium containing $1.0\;mg/\;{\ell}$ of BA and $0.1\;mg/\;{\ell}$ of NAA.

  • PDF

Efficient Plant Regeneration from Mature Seed-derived Callus of Italian ryegrass (Lolium multiflorum Lam.) (이탈리안 라이그래스의 성숙종자 유래 캘러스로부터 효율적인 식물체 재분화)

  • Woo, Hyun-Sook;Lee, Sang-Hoon;Lee, Dong-Gi;Kim, Jin-Soo;Won, Sung-Hye;Lee, Byung-Hyun
    • Journal of Plant Biotechnology
    • /
    • v.31 no.1
    • /
    • pp.43-48
    • /
    • 2004
  • As an initial step for future genetic manipulations to improve forage characteristics of Italian ryegrass (Lolium multiflorum Lam.), an efficient tissue culture system was established and the factors affecting plant regeneration were evaluated. MS medium containing 5mg/L 2,4-D was optimal for embryogenic callus induction from mature seed and had a strong effect on successive plant regeneration. The plant regeneration frequency was observed at above 70% when embryogenic calli induced were transferred to N6 medium supplemented with 1mg/L 2,4-D and 5mg/L BA. Among several basic media tested, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Genotype was an important factor in plant regenerability. 'Jeanne' showed the highest regeneration frequency of 73%. A short tissue culture period and high-frequency regeneration system established in this study will be useful for molecular breeding of Italian ryegrass through genetic transformation.

Effect of plant growth regulators on plant regeneration from the Belamcanda chinensis (범부채 (Belamcanda chinensis)의 식물체 재분화에 미치는 식물생장조절제의 영향)

  • Kwon, Hye-Kyoung;Yoo, Kyoung-Hwa;Yoon, Eui-Soo
    • Journal of Plant Biotechnology
    • /
    • v.37 no.3
    • /
    • pp.337-342
    • /
    • 2010
  • To establish the optimum conditions of in vitro plant regeneration, the leaf, rhizome, and root explants of Belamcanda chinensis were cultured on the MS medium supplemented with different concentration of 2,4-D and BA. The callus induction was more effective in the root explants than the leaf and rhizome explants, and was the best on MS medium containing 3.0 mg/L 2,4-D or 1.0 mg/L 2,4-D and 3.0 mg/L BA. The highest numbers of shoots were regenerated when callus were cultured on MS medium containing 3.0 mg/L 2,4-D for 4 weeks. However, the multiple shoots were induced on MS medium supplemented with the combination of 2,4-D and BA. The normal root formation from shoot was effective on the MS medium lacking any plant growth regulators. For acclimatization, the regenerated plantlets were cultured on MS medium without sucrose and plant growth regulators for 2 weeks, and then transferred to the pot.

Immature Pollen-Derived Plant Regeneration in Anther Cultures of Ranunculus japonicus Thunb (미나리아재비(Ranuculus japonicus Thunb.)의 약배양에 의한 미숙 화분 유래의 식물체 재분화)

  • 고정애;김영선;김명준;은종선
    • Korean Journal of Plant Tissue Culture
    • /
    • v.21 no.5
    • /
    • pp.293-297
    • /
    • 1994
  • In order to induce immature pollen derived plants, anthers of Ranunculus japonicus Thunb. were cultured on Murashige and Skoog's medium supplemented with various combinations of auxins and cytokinins. The combinations of NAA and BA were more effective than those of 2,4-D and kinetin in the formation of calli and embryos. Up to 5t5% of the anthers cultured on medium containing 0.5 mg/L NAA and 1.0 mg/L BA gave rise to plantlets. The most suitable stage for anther culture in the induction of calli and/or embryos from immature pollens was at the uninucleate and early binucleate stage (3 days before anthesis). Immature pollens developed into embryos by repeated division of the vegetative nucleate after 60days of culture.

  • PDF

Effect of Subculture on Plant Regeneration in Rice Callus Culture (벼 Callus의 계대배양(繼代培養)이 식물체(植物體) 재분화(再分化)에 미치는 영향(影響))

  • Sohn, Jae Keun;Lee, Seong Mok;Kim, Kyung Min
    • Current Research on Agriculture and Life Sciences
    • /
    • v.9
    • /
    • pp.21-28
    • /
    • 1991
  • The effect of subculture intervals and passages on plant regeneration from seed-derived callus was determined. Regeneration capacity of callus varied with rice cultivars and subculture intervars tested. The callus subcultured every 2 weeks produced more plants than that of 4 weeks. The calli from a Tongil-type rice cultivar, Milyang 23, lost easily their regeneration ability when the calli were subcultured every 2 weeks and 4 weeks. The callus induced from a japonica cultivar, "Yeongdeogbyeo", showed to maintain high frequency(>70%) of plant regeneration when it was subcultured every 2-week intervals. Casein hydrolysate supplemented in callus induction medium enhanced callus growth and its regeneration. High frequency of plant regeneration was obtained from the calli transferred on $N_6$ medium supplemented with kinetin(2mg/1) and NAA(1mg/1). The subcultured calli in the medium supplemented with casein hydrolysate(2 g/1), myo-inositol(200mg/1) and thiamine-HCl(2mg/1) increased the frequency of embryogenic callus formation and plant regeneration.

  • PDF

Plant Regeneration and Genetic Diversity of Regenerants from Seed-derived Callus of Reed (Phragmites communis Trinius) (갈대(Phragmites communis Trinius) 성숙종자를 이용한 기내 식물체 재분화와 재분화체의 유전적 다양성)

  • Ryu, Jaihyunk;Kim, En-Hwan;So, Hyun-Su;Chung, Mi-Young;Song, Won-Seob;Bae, Chang-Hyu
    • Korean Journal of Plant Resources
    • /
    • v.26 no.2
    • /
    • pp.320-327
    • /
    • 2013
  • This study was carried out to address an efficient in vitro regeneration system from seed-derived callus of Phragmites communis, and to evaluate genetic variations of the regenerants using ISSR markers. Shoot regeneration via calli was greatly influenced by N6 medium compared with MS medium, and plant regeneration frequency was 90% in N6 supplemented with BA 0.25 mg/L and BA 0.5 mg/L. According to ISSR analysis of the thirty regenerants, out of 94 loci detected overall, 16 were identified to be polymorphic with a rate (PR) of 17.0%. The mean gene diversity (h) of different in vitro condition was 0.03 and ranged from 0.008 for N6 with BA 5 mg/L, to 0.040 for MS with IAA 0.1 mg/L+kinetin 2 mg/L. The results indicate that the regenerants have a low genetic variation, and ISSR analysis is effective to detect genetic variation of regenerants.