• Korean Journal of Plant Resources
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• v.9 no.2
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• pp.177-181
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• 1996

This study was carried out to obtain basic data for technical inprovement about cultivation according to micrometerological changes under various mulching materials in Dendropanox morbifera LEV. The results are summarized as follows. Transparent polyethylene film and black polyethylene film mulching materials increased the soil porosity and reduced change of the soil moisture content. The rate of missing plant during overwinter increased in the control plot. Growth of Dendropanox morbifera LEV, was accelerated by transparent polyethylene film and black polyethylene film mulched.

• Korean Journal of Plant Tissue Culture
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• v.25 no.1
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• pp.63-68
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• 1998

Multiple shoots obtained in MS medium suppler with 5.0 mg/L BA though shoot-tip culture. The frequency of vitrified shoot was lower on Bacto-agar medium than on Gelrite as gelling agent. Addition of activated charcoal at concentrations of 0.1~0.3% reduced vitrification and markedly increased shoot growth, and formation and growth of roots, but significantly reduced the number of shoots formed. The ratio of fresh weight to dry weight was decreased by increasing light intensity and agar concentration. Eight-tenths times of macroelement of MS medium was observed to be effective for shoot formation. Addition of IAA effectively promoted shoot formation in both shoot tip and node-bud explants. Supplement of 5.0 mg/L BA, 0.3 mg/L IAA to MS medium was most effective in shoot proliferation on shoot tip and node-bud explants.Multiple shoots obtained in MS medium suppler with 5.0 mg/L BA though shoot-tip culture. The frequency of vitrified shoot was lower on Bacto-agar medium than on Gelrite as gelling agent. Addition of activated charcoal at concentrations of 0.1~0.3% reduced vitrification and markedly increased shoot growth, and formation and growth of roots, but significantly reduced the number of shoots formed. The ratio of fresh weight to dry weight was decreased by increasing light intensity and agar concentration. Eight-tenths times of macroelement of MS medium was observed to be effective for shoot formation. Addition of IAA effectively promoted shoot formation in both shoot tip and node-bud explants. Supplement of 5.0 mg/L BA, 0.3 mg/L IAA to MS medium was most effective in shoot proliferation on shoot tip and node-bud explants.

• Microbiology and Biotechnology Letters
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• v.39 no.4
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• pp.324-329
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• 2011

Eight endophytic fungal strains were isolated from the roots of Calystegia soldanella from the western coast of South Korea. The culture filtrate of the eight endophytic fungi were applied to waito-c rice seedlings in order to verify potential plant growth promotion activities. The results of bioassay indicated that the Cs-9-7 fungal strain possessed the highest plant growth promotion activity. Fungal culture filtrates were analyzed to verify secondary metabolites using gas chromatography and mass spectroscopy with selected ion monitoring (GC/MS-SIM). The culture filtrate of the Cs-9-7 fungal strain was confirmed to contain gibberellins GA3 (1.229 ng/mL), GA4 (3.535 ng/mL), GA7 (1.408 ng/mL) and GA12 (0.378 ng/mL). Polymerase chain reactions (PCR) were performed so as to determine the internal transcribed spacer (ITS) regions for the identification of isolated strains with universal primers ITS-1 and ITS-4. The Cs-9-7 fungal strain, isolated from the root of C. soldanella, has been named Aspergillus tubingensis Cs-9-7.

• Korean Journal of Plant Tissue Culture
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• v.21 no.1
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• pp.47-53
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• 1994

The effects of light on the production of anthocyanin and betacyanin in cell suspension cultures of Vitis vinifera and Phytolacca americana were investigated. The cell growth of V.vinifera was little affected by exposure to light, but that of P.americana was markedly increased by light than in the dark In suspension cultures of V vinifera maximum accumulation of anthocyanin was observed during the stationary phase in continuous light By contrast, in suspension cultures of R americana, accumulation of betacyanin occured in parallel with cell division which showed two peaks after 4 days and 8 days of culture in continuous light whereas in continuous dark accumulation of anthocyanin and betacyanin did not occured However treatment of light interrupting for l, 12, and 24 h after 4 days in cell suspension. cultures of remarkably showed a slight anthocyanin accumulation, but after 8 days of culture remarkably accumulated by light interrupting for more than 12 h. In cultures of P. americana, the light treatment was more effective at 4th day than at 7th day after culture, but betacyanin accumulation was decreased again in the dark after light treatment These result indicate that the difference of light responses exist between the V.vinifera and the betacyanin of P. americana though cell suspension culture systems.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.2
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• pp.182-188
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• 1997

The study was carried out to determine an optimum concentration of plant growth regulators(ABA and kinetin) on reducing NaCl toxicity during germination in rice. Plant height of a japonica cultivar Ilpumbyeo in ABA 10$^{-5}$ M was increased, while all treatments of kinetin had no clear effects on increasing the plant height. However, other rice cultivars showed slightly different reaction by PGRs compared with Ilpumbyeo. Germination rate of four rice cultivars was ranged from 53.1 to 58.2％ in NaCl 1.3％. All treatments of kinetin accelerated germination rate of tested rice cultivars except Dasanbyeo compared with NaCl 1.3％ treatment. The higher concentration of ABA and kinetin treatment induced the higher starch content, and while the lower concentration of two plant growth regulators induced the higher sugar content in all four rice cultivars. The free proline content of rice seedlings was highest in Ilpumbyeo among the tested cultivars under NaCl 1.3％ treatment.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.29-29
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• 2019

상동나무(Sageretia theezans)는 갈매나무과 상동나무속 식물로 특이하게 늦가을에 꽃이 피고 봄에 열매가 익는 수종이며, 최근 암세포 생장억제 효과가 입증되고 제주와 전남 남해안 지역에서 예로부터 열매를 식용 및 약용하여 민속식물로서의 가치가 크다. 본 연구에서는 향후 약용소재로서의 가치가 높은 상동나무의 대량증식을 위하여 식물생장호르몬의 처리에 따른 효과적인 증식법을 구명하고자 실시하였다. 상동나무 삽수묘는 고흥, 완도 일대에서 2019년 3월 19일에 2년생 가지를 수집하였으며, 3월 20일 삽목을 실시하였다. IAA(250, 500, 1,000 ppm), IBA(250, 500, 1,000 ppm), NAA(250, 500, 1,000 ppm), 루팅(200배) 수용액에 50개의 삽수묘를 3분간 절단기부를 침지 후 모래에 치상하였으며, 무처리구는 바로 치상하였다. 각 삽수상은 온실에 보관 후 1일 2회씩 관수 하였으며 120일간 유지하였다. 그 결과 전체 66.4%의 발근율을 보였고 캘러스와 뿌리의 발달은 평균 17.5 mm의 길이를 보였다. IBA 처리구가 전반적으로 높은 발근율을 보였는데 그 중 500 ppm 처리구에서 가장 많은 41개 삽수묘의 부정근이 형성되어 82%의 발근율을 보였고, 평균 20.2 mm 길이의 뿌리가 생성되었다. IBA 1,000 ppm처리구에서도 38개의 삽수묘에서 캘러스 및 부정근이 형성되어 76%의 발근율을 보였다. 뿌리의 발달은 평균 18.4 mm로 IBA 500 ppm에 비해 길이가 짧았으나 뿌리의 길이가 삽수묘간 차이가 커 가장 긴 개체가 32.3 mm로 나타났다. NAA 500 ppm에서도 높은 발근율을 보였는데 32개의 삽수묘에서 부정근이 형성되어 64%의 발근율로 나타났다. 뿌리의 길이는 18.9 mm로 IBA 1,000 ppm에 비해 발근율은 낮았으나 뿌리의 발달은 평균 0.5 mm 높은 것으로 나타났다. 무처리구는 29개의 삽수묘가 캘러스 및 부정근이 형성되었으며 58%의 발근율로 나타났으며 IAA, IBA, NAA 처리구 모두 무처리구에 비해 높은 발근율을 보였다. 생장호르몬 당 발근율을 보면 IBA는 500 ppm, NAA는 500 ppm, IAA는 1,000 ppm에서 가장 높은 발근율을 보였다. 루팅 수용액 처리구는 60%의 발근율을 보여 루팅 200배 수용액 침지와 무처리는 비슷한 수치의 발근율로 나타나 루팅은 상동나무 삽목 발근촉진제로 효과가 없는 것으로 확인되었다.

• Journal of Life Science
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• v.15 no.3 s.70
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• pp.499-504
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• 2005

This experiment was conducted to identify the effect of several plant growth retardants on growth of Sedirea japonica seedlings cultured in vitro and their changes of invertase activities. When seedlings of Sedirea japonica were treated with ancymidol and paclobutrazol, as the concentrations were increased, leaf length was gradually shortened and leaf width became wider than that of control. On the other hand, root length was shorter, but the number of root and the root's diameters were greatly increased, compared with control. In 0.05mg/L uniconazole, growth of leaf and root were enhanced, compared with the control and higher concentrations of uniconazole. As concentration of each growth retardants was increased, leaf shape became round and smaller. Both soluble acid invertase activity and soluble alkaline invertase activity in leaf were decreased in higher concentrations of each growth retardant, but those of the root were contrary to those of the leaf. To confirm the estimated invertase activities, starch content of leaf was higher in low concentration treatments in each growth retardant, but in the root was contrary to content that of the leaf.

• Applied Biological Chemistry
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• v.47 no.3
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• pp.361-365
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• 2004

Chitin and its derivatives have been introduced to tomato seedlings by direct mixing of chitin, chitosan, or chitooligosaccharides (CO) with soils, watering of chitosan or CO solutions to soils, or chitosan-coating of tomato seeds as a biomodulator of plant growth, and the growth of tomato plants was measured 30- and 45-day after seeding in the pots. The treatment of chitin and its derivatives promoted the growth of tomato plants in plant heights, shoot weight, maximum leaf length and number of leaves. By comparison in the contents of cellular inorganic nutrients in the plants, it was found out that chitin or chitosan treatments accelerated nitrogen and potassium uptake but inhibit calcium uptake, suggesting that chitin and its derivatives modulate absorption of inorganic nutrients through plant roots from environment.

• Korean Journal of Plant Resources
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• v.29 no.4
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• pp.385-392
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• 2016

This research was performed to develop seminal propagation method of Saururus chinensis (Lour.) Baill. by conducting a rigorous germination study. Well-selected seeds were dry-stored at 4 ± 1.0℃ during the experiment. To study dormancy type, non-stored seeds were analyzed by embryo observation, germination test and detecting for any difficulties in seed coat to absorb moisture. Then to improve germination, seeds were submerged for 24 hours in a solution of varying concentrations containing one of plant growth regulators and minerals. According to research, fleshly matured seeds had an undifferentiated embryo and had a low germination rate below 5%. In addition, water submersion led to moisture absorption, embryo in the seeds grew and germinated so it was deemed morphophysiological dormant seeds. Percent germination (PG) and germination energy (GE) was greatly improved by soaking in plant growth regulators and minerals for 24 hours. Especially, 500 ㎎/L GA₃ treatment resulted in the highest GE as 46.1%. KNO₃ meaningfully improved PG (54.3∼57.7%) at 10∼20 mM but effect of minerals on germination acceleration as GE were negatively impacted in all concentrations.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.1
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• pp.31-40
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• 1999

This study was conducted to investigate the effects of rhizosphere bacterium and pathogenic fungi on the growth of corn(Zea may L.) in continuous corn cultivation soil(CCCS) and non-continuous cultivation soil(NCCS). Corn was established by seeding into pots of 30 cm in diameter and 50 cm in depth containing 1 : 1 mixture of soil and vermiculite. Rhizobacterium and pathogenic fungi were inoculated into the soils. The field experiment was carried out at the Animal Research Station, College of Agriculture, Chonnam National University. Sample of corn was taken from each pot at 50 days and 90 days after sowing. Corn was cultivated in a vinyl house with three replications under natural daylight conditions. The bacterium used in this study was Bacillus subtilis. B. subtilis was directly isolated and identified from forage rhizosphere soil. Dry matter(DM) of coron plant in treatment without B. subtilis was lower than that in treatment of B. subtilis. DM of corn plant inoculated with B. subtilis was higher than that of corn inoculated with pathogenic fungi in both CCCS and NCCS. DM of corn plant in NCCS was more increased than that in CCCS. The effect of B. subtilis inoculation on the growth of corn was better in NCCS than in CCCS. However, DM of corn plant was apparently decreased by the inoculation of the pathogenic fungi in both CCCS and NCCS.