Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Growth Retardants on Growth and Invertase Activity of Sedirea japonica Seedlings in vitro

생장억제제 처리가 나도풍란(Sedirea japonica) 유묘의 생장과 invertase 활성에 미치는 영향

  • Jee Sun Ok (Department of Biotechnology, Joongbu University) ;
  • Cho Dong Hoon (Department of Horticulture, Kyungpook National University)
  • Published : 2005.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

This experiment was conducted to identify the effect of several plant growth retardants on growth of Sedirea japonica seedlings cultured in vitro and their changes of invertase activities. When seedlings of Sedirea japonica were treated with ancymidol and paclobutrazol, as the concentrations were increased, leaf length was gradually shortened and leaf width became wider than that of control. On the other hand, root length was shorter, but the number of root and the root's diameters were greatly increased, compared with control. In 0.05mg/L uniconazole, growth of leaf and root were enhanced, compared with the control and higher concentrations of uniconazole. As concentration of each growth retardants was increased, leaf shape became round and smaller. Both soluble acid invertase activity and soluble alkaline invertase activity in leaf were decreased in higher concentrations of each growth retardant, but those of the root were contrary to those of the leaf. To confirm the estimated invertase activities, starch content of leaf was higher in low concentration treatments in each growth retardant, but in the root was contrary to content that of the leaf.

본 연구는 몇 가지 생장억제제를 배지에 처리하였을 때 기내 배양한 나도풍란 유묘의 생장과 그들의 invertase활성 검정을 위하여 실시하였다. 나도풍란에 식물생장억제제 인 ancymidol과 paclobutrazol을 각각 배양용 배지에 첨가했을 때, 농도가 높을수록 엽장이 감소한 반면, 엽폭은 상대적으로 증가하였고, 근장은 짧았으나 근수 및 근직경은 증가하였다 Uniconazole 0.05 mg/L에서는 잎과 뿌리생장이 대조구보다 오히려 촉진되었다. 처리한 모든 종류에 있어서 그 농도가 높아짐에 따라 잎 모양은 원형으로 변하면서 작아졌다. Invertase 활성을 알아보기 위 한 soluble acid invertase 활성과 soluble alkaline invertase활성은 잎에서는 생장억제제 농도가 낮은 처리에서 높았고, 뿌리에서는 정반대의 경향이었다. 지상부 및 지하부의 전분함량은 잎에서는 invertase 활성이 높았던 저농도에서 높았으며, 뿌리에서는 invertase 활성이 높았던 고농도에서 높았다.

Keywords

References

  1. Bechtel, H. P. Cribb, and E. Launert. 1992. The manual of cultivated orchid species. 3rd ed. Blandford. London
  2. Boe, A. A., T. S. Lee, D. D. Tapido, and T. J. Banks. 1973. The effect of SADH on radish. HortScience. 8, 497-498
  3. Broughton, W. J., A. J. McComb. 1971. Changes in the pattern of enzyme development in gibberellin-treated pea internode. Ann Bot. 35, 213-228
  4. Byun, J. K., S . Y Wang. and G. J. Steffens. 1984. Controlling Plant Growth Via the Gibberellin Biosynthesis System; II. Carbohydrates Alterations by $GA_3$ and Paclobutrazol in Apple Seedlings, HORTICULTURE ABSTRACTS, 2, 68-69
  5. Chaplin, M. F. and J. F. Kennedy. 1986. Carbohydrate analysis. A practical Approac JRl. Press, oxford and washington D.C
  6. Chung, J. D., C. K. Chun., S. S. Kim .1983. Asymbiotic Germination and Plant Culture of Aerides japonicum (1) Selection of Suitable Medium and Culture Condition for Germination and Effect of Hyponex, Peptone, and Several Nutrient Supplements on Growth of Seedlings. HORTICULTURE ABSTRACTS, 1, 52-53
  7. Chung, J. D., Y. K. Park, H. Y. Kim, S. O. Jee, J. C. Koh. 1999. Effects of Plant Growth Retardants on the Growth of Bletilla striata in vitro. J. Kor. Soc. Hart. Sci. 40, 485-488
  8. Dyson, P. W. 1972. Effect of cycocyl (2-chloroethyltrimethyl ammonium chloride) and Alar (N-dimethylaminosuccinamic acid) on the yields of carrots. J. Hort. Sci. 47, 215-220
  9. Graebe, J. E. 1987. Gibberellin biosynthesis and control. Annu. Rev. Plant Physiol. 318, 419-465
  10. Jee, S. O., J. D. Chung, Y. K. Park, H. Y. Kim. 2000. Effects of growth retardants on the morphogenesis and GA-like substance activity of Bletilla striata in vitro. J. Kor. Soc. Hort. Sci. 41, 409-414
  11. Kim, H. Y. 1998. Effects of uniconazole treatment on the growth and flowering of Cymbidium Pine Clash 'Moon Venus' and Cym. Green Sour 'A One'. Kor. J. Hort. Sci & Tech. 16, 40-41
  12. Kim, H. Y., H. Watanabe and Y. Suzuki. 1992. Effect of growth retardant uniconazole on the floral formation of Zinnia elegance Jacq. J. Japan. Soc. Hort. Sci. 61, 603-608 https://doi.org/10.2503/jjshs.61.603
  13. Kim, H. Y. and Y. Suzuki. 1989. Changes in assimilated BC distribution and soluble acid invertase activity of Zinnia elegance induced by uniconazole an inhibitor of gibberellin biosynthesis. Plant physiol. 90, 316-321 https://doi.org/10.1104/pp.90.1.316
  14. Larson, R. A. 1985. Growth regulators in floriculture. Hort. Rev. 7, 399-481
  15. Lee, C. B. 1989. Illustrated flora of Korea. Hyangmunsa. Seoul
  16. Lee, J. 1985. The wild orchid of korea. Jangsung publisher. Seoul
  17. Lowry, O. H., N. J. Rosebrough., A. J. Farr., R. J. Randall. 1951. Protein measurement with the folin phenol reagent. J. BioI. Chem. 193, 265-275
  18. McCollum, T. G., D. J. Huber., D. J. Cantliffe. 1988. Soluble sugar accumulation and activity of related enzymes during musk melon fruit development. J. Am. Soc. Hort. Sci. 111, 399-403
  19. Morris, D. M., E. D. Arthur. 1984. An association between acid invertase activity and cell growth during leaf expansion in Phaseolus vulgaris J. J. Exp. Bot, 35, 1369-1379 https://doi.org/10.1093/jxb/35.9.1369
  20. Morris, D. M., E. D. Arthur. 1985. Effects of gibberellic acid on pattern of carbohydrate distribution and acid invertase activity in Phaseolus vulgaris. Physiol. Plant 65, 257-262 https://doi.org/10.1111/j.1399-3054.1985.tb02392.x
  21. Paek, K. Y., H. Y. Kim., T. J. Kim., S. K. Park., K. C. Son and J. H. Suh. 1995. From beginning to management of Cymbidium culture. The nongmin press. Seoul
  22. Park, J. S. 2000. Studies on habitat environment and flowering physiology of Cymbidium goeringii. PhD thesis, Kyungpook national university, Daegu
  23. Park, Y. K. 2000. Effect of plant growth retardants on the growth of Aerides japonicum and Bletilla striata cultured in vitro. MS thesis, Kyungpook national university, Daegu
  24. Ricardo, C.C.P., T. Rees. 1970. Invertase activity during the development of carrot roots. Phytochemistry 23, 239-247
  25. Seitz, K., and A. Lang. 1968. Invertase activity and cell growth in lentil epicotyls. Plant Physiol. 43, 1075-1082 https://doi.org/10.1104/pp.43.7.1075
  26. Somogyi, M. 1952. Notes on sugar determination. J. BioI. Chem. 195, 19-23
  27. Steffens, G. I., S. Y. Wang, M. Faust, J. K. Byun. 1985. Controlling plant growth via the gibberellin biosynthesis system. I. Growth parameter alterations in apple seedlings. Physiol. Plant 63, 163-168 https://doi.org/10.1111/j.1399-3054.1985.tb01897.x
  28. Wang, Y. T., K. H. Hsiao and J. J. Gregg. 1992. Antitranspirant, Water stress and Growth Retardant Influence Growth of Golden Pathos. HortSience. 27, 222-225