• Title/Summary/Keyword: 시료전처리

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Immuno-stimulating and anti-metastatic activities of the polysaccharides isolated from Angelica gigas (참당귀로부터 분리한 다당의 면역증진 활성과 항전이 활성)

  • Son, Seung-U;Shin, Kwang-Soon
    • Korean Journal of Food Science and Technology
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    • v.53 no.3
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    • pp.304-312
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    • 2021
  • The present study aimed to develop new physiologically active ingredients from Angelica gigas. The polysaccharides purified from A. gigas, AGE-2c-I, showed potent anti-complementary activity in a dose-dependent manner. C3 activation products were identified through crossed immuno-electrophoresis using anti-human C3 antibodies and the anti-complementary activity of AGE-2c-I under Ca++-free conditions suggests that AGE-2c-I may induce complementary activation via both alternative and classical pathways. In addition, AGE-2c-I augmented the production of various cytokines, such as interleukin (IL)-6, IL-10, IL-12, and tumor necrosis factor-α, by peritoneal macrophages. Furthermore, intravenous (i.v.) administration of AGE-2c-I dose-dependently enhanced natural killer cell cytotoxicity against YAC-1 lymphoma. In experimental lung metastasis, prophylactic i.v. administration of AGE-2c-I inhibited lung metastasis by 58% at 100 ㎍/mouse. From the above results, we suggest that AGE-2c-I purified from A. gigas has potent immune system-stimulating activities, and is a potentially promising food ingredient beneficial to human health.

Degradation Pattern and Rate of Some Pesticides in Soils -Part I. Degradation Pattern and Rate of Parathion in Soils- (토양처리(土壤處理) 농약제(農藥劑)의 분해율(分解率)에 관한 연구(硏究) -제1보(第一報). Parathion의 토양중(土壤中) 분해(分解)에 대하여-)

  • Lim, Sun-Uk;Kang, Kyu-Yung;Choi, Yong-Lak
    • Applied Biological Chemistry
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    • v.26 no.4
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    • pp.239-247
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    • 1983
  • The effects of some soil conditions on the degradation rate and decomposing pattern of parathion were investigated and the obtained results are summarized as follows: Parathion degraded more rapidly in flooded soils than in non-flooded, in wet soils than in dry soils under non-flooded soils. The degradation rates in paddy and upland soils increased at high temperature than low temperature, higher pesticide concentration than low concentration and higher soil pH level. Parathion in paddy and upland soils was more persistent under soil sterilization than under non-sterilization and degraded rapidly in glucose application. Parathion was more persistent in upland soils than paddy soils under several factors described above. The metabolites identified from the paddy and upland soils by TLC include para-oxon (Rf 0.5), aminoparathion(Rf 0.27), p-nitrophenol(Rf 0.2), p-aminophenol(Rf 0.15). Soil enzyme, acid phosphatase activities decreased more at flooded soils than non-flooded, higher pesticide concentration than low concentration and higher soil pH level and the activity in glucose application was increased. Soil enzymes, urease and dehydrogenase activity decreased more at higher pesticide concentration than low concentration. Comparing with soil enzyme activity in paddy and upland soil, the former was higher than the latter.

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Chemical Forms and Release Potential of Heavy Metals from the Lime Treated Sediments (석회 처리에 의한 오염 퇴적물 내 중금속의 형태 변화 및 용출 가능성)

  • Park, Gil-Ok;Jun, Sang-Ho
    • Korean Journal of Ecology and Environment
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    • v.41 no.2
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    • pp.166-173
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    • 2008
  • Chemical forms and release potential of heavy metals were studied in the lime treated sediment of lake Chungcho. Chemical forms of heavy metals were analyzed using a sequential extraction method, and release potential of heavy metals was evaluated by the ratio of the content of labile forms to total metal one. Dominant form of Cd, Cu, Pb, and Zn in the untreated sediments was organic/sulfidic form that is stable in the reducing environment such as the bottom of Lake Chungcho. With liming of the sediment, the chemical forms of studied metals were greatly changed from organic/sulfidic form to adsorbed and reducible form, especially Cd and Cu to adsorbed and reducible form, but Pb and Zn to reducible form. It is believed that increase of unstable form of heavy metals in the sediments by liming was caused by the increase of pH of the pore water at the expense of organic/sulfidic form. Thus, we concluded that the liming approach currently used in the treatment of dredged sediments might cause the increase of labile form which is easily dissolved, and may increase the release of metals from the sediment into overlying water.

Effects of Pretreatment and Storage Condition on the Quality of Canned Boiled Oyster (전처리와 저장조건이 굴 통조림의 품질에 미치는 영향)

  • NO Rak-Hyeun;SEONG Dae-Whan;YANG Han-Serb;BYUN Dae-Seok
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.14 no.3
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    • pp.130-138
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    • 1981
  • Discoloration of canned boiled oysters during storage is one of the serious problems which affect the quality of the products as well as the nutritive value. Usually the factors influencing the quality of canned boiled oysters are the process of pretreatments and the storage temperature of the products. In the present work, the changes of the total chlorophylls and carotenoids in the meat and the viscera of oysters were determined in order to make certain the procedure of the discoloration. In addition, the amino-N and the available lysine as factors of the nutritive value were also checked. In case of treatment with additives, direct addition of syrups containing additives just before seaming or soaking boiled oysters into the solution of additives seemed to have mild effects on retardation of discoloration. The migration of carotenoids from the viscera into the meat was faster than that of chlorophyll resulting in yellowing of the Products preceded greening caused by the chlorophylls. Treatment with $0.5\%\; Na_{2}EDTA\;of\;2.5\%$ brine retarded discoloration and available lysine loss of the Products while sodium-polyphosphate accelerated them. It was probably due to that sodium-polyphosphate could affect the softening or breakdown of the muscle of oysters. But most of all, storage temperature of canned boiled oysters seemed to be the major factor influencing the discoloration and nutritive loss of the products.

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Effects of Hair Dyeing and Change of Hair Texture by Indigo Dye (인디고 염료에 의한 모발 염색효과와 모질의 변화)

  • Kim, Ju-Sub
    • Journal of the Korean Applied Science and Technology
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    • v.38 no.1
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    • pp.9-18
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    • 2021
  • This study was aimed to investigate the effects of hair dyeing and changes of hair quality by the application of indigo dye. Methods: Indigo dye was used after water bath. After application of the dye to healthy and bleached hair samples, they were treated with heat and remained naturally by different times. Each sample was measured before and after the application and compared for the analysis. To investigate the effects of dyeing, L⁎, a⁎, and b⁎ values and bleaching degree were measured using by color-difference meter. To understand the changes of hair quality, absorbance and gloss were measured using by tensile strength and methylene blue. Results: Upon the results of dyeing, all the samples showed the significant dyeing effects before and after the application in terms of L⁎, a⁎, and b⁎ values. With respect to the bleaching effects, it showed the greatest change on Day 1 and did not show any changes from Day 3. For the measurement of tensile strength, mean values of all the samples were increased. However, the results were not significant, statistically, demonstrating no change of hair quality. No statistically significant results were found in the samples except healthy 7L(3) sample upon the absorbance results using methylene blue. Gloss of samples was changed upon the statistical analysis results. Conclusion: Indigo dye showed the hair dyeing effects, significantly, while gloss was changed and tensile strength and absorbance were not changed in terms of hair quality. Further studies are required on the processing with a variety of dyes and on the measurements for reliability and objectivity.

Distribution characteristics of perfluorinated compounds in major river water and sediment (우리나라 주요 하천수 및 퇴적토에 축적된 과불화화합물의 분포특성)

  • Yeo, Min-Kyung;Hwang, Eun Hye;Jeong, Gi Ho
    • Analytical Science and Technology
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    • v.25 no.5
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    • pp.313-323
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    • 2012
  • We have developed analysis method of PFCs in river water and sediment, and determined seven species of PFCs such as PFBS, PFHxS, PFOS, PFHpA, PFOA, PFNA and PFDA. Water and sediment samples were collected from 21 and 13 different sites along the Nakdong, Seomjin and Nam River, respectively. The water samples were pretreated with HLB cartridge and sediment samples were concentrated after extracted by sonication, and the levels of PFCs were determined by LC-MS/MS. The coefficient of determination ($R^2$) values of calibration curves were higher than 0.99. The method detection limits ranged 0.09~0.63 ng/L in water and 0.013~0.020 ng/g in sediment. The recovery rates of PFCs was found to be 74~98% for water and 87~111% for sediment. PFOA was the major species in water samples and followed by PFHpA and PFOS. In sediment, PFOA, PFOS and PFDA showed similar levels. Both water and sediment samples collected from the Nakdong River showed the highest concentrations of PFCs among the three rivers.

Considerations of Permeability of Converter Slag for Recycling (재활용을 위한 전로슬래그의 투수성 고찰 (I))

  • 이광찬;이문수
    • Journal of the Korean Geotechnical Society
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    • v.15 no.4
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    • pp.69-83
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    • 1999
  • The permeability of converter slag, replacing material of sand mat on improving soft clay foundation, was evaluated in the laboratory. The effects of grain size, flow water time and aging were investigated using sea and fresh water. In the case of converter slag submerged with fresh water, the coefficients of permeability in A and B samples less than 10 mm grain sizes were measured as $6.52\times10^{-2}cm\; per\; sec\; and\; 5.99\times10^{-1}/cm$ per sec respectively, while they were $1.88\times10^{-2}/cm\; per\; sec,\; 3.86\times10^{-1}/cm$ per sec respectively under sea water condition. Also, the condition of turbulent flow may exit and was experimentally identified from the relationship between hydraulic gradient and seepage velocity. After 100 days under sea water condition, the coefficients of permeability of A and B samples decreased ten times than initial values. The reduction of permeability coefficient was considered to result from the filling of voids in high-calcium quicklime(CaO).

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Determination of Selenium in Milk by ICP-OES (ICP-OES를 이용한 우유의 Selenium 분석)

  • 김효중;박종길;신정걸;백영진
    • Food Science of Animal Resources
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    • v.23 no.4
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    • pp.356-360
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    • 2003
  • The purpose of this paper was to determine the quantity of selenium in milk by inductively coupled plasma optical emission spectrometry. The sample was digested in teflon vessel containing nitric-hydrogen peroxide acid mixture. After digestion, the sample is treated with additional hydrochloric acid. Total selenium was reduced with sodium borohydride and concentrated hydrochloric acid in a simplified hydride generation(HG) manifold. The optimum conditions of HG are 2 M for HCl, 1.5% for NaBH$_4$, 1.2 mL/mim for sample flow. Recovery rates by the standard addition method were 88.0% at 10 ppb and 92.2% at 10 ppm. The relative standard deviations were 4.8 and 3.2%, respectively. This method showed a good accuracy and precision. And so it was highly suitable for determination of small quantity of selenium in milk.

Simultaneous Determination of Vitamin $D_3\;and\;K_1$ in Infant Formula by Column-switching High Performance Liquid Chromatography with UV Detection (Column-switching HPLC를 이용한 성장기용 조제식 중 비타민 $D_3,\;K_1$의 동시분석)

  • Kwak, Byung-Man;Ahn, Jang-Hyuk;Chang, Chi-Hoon
    • Korean Journal of Food Science and Technology
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    • v.37 no.6
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    • pp.1024-1027
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    • 2005
  • Rapid and simple method was developed for simultaneous determination of vitamins $D_3\;and\;K_1$ contents in infant formula. Contents of vitamins $D_3\;and\;K_1$, extracted by column-switching HPLC with reversed phase column using enzymatic hydrolysis and organic solvent, in CRM determined by developed method were within certified ranges of standard values.

Characteristics of the release of chromium, copper, and arsenic from CCA-treated wood exposed to the natural environment (자연환경에 노출된 CCA 방부목재로부터 크롬, 구리 및 비소의 용탈 특성)

  • Koo, Jinhoi;Song, Byeongyeol;Kim, Hekap
    • Analytical Science and Technology
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    • v.21 no.1
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    • pp.1-8
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    • 2008
  • This study was conducted to investigate the characteristics of the leaching of metal components from CCA-treated wood during outdoor exposure. CCA-treated wood specimens were placed horizontally or buried vertically into the soil, and then exposed to the natural environment for a year. Wood samples were collected from the side of the horizontal wood specimens using a drill and saw dust samples were collected at the end of the exposure. Soil samples were also obtained around the wood specimens and at different depths of the posts. Wood and soil samples were analyzed for metals using an atomic absorption spectrometer. Monthly metal concentrations varied greatly and more metals were released when wood specimens were exposed vertically than horizontally. Arsenic was released from the wood by 80 % of the intial content. In addition, more leaching was observed from the zone below the ground than above the ground, and soil around the posts was contaminated with metals released from CCA-treated wood.