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Simultaneous Determination of Vitamin $D_3\;and\;K_1$ in Infant Formula by Column-switching High Performance Liquid Chromatography with UV Detection  

Kwak, Byung-Man (Research and Development Center, Namyang Dairy Products Corporation)
Ahn, Jang-Hyuk (Research and Development Center, Namyang Dairy Products Corporation)
Chang, Chi-Hoon (Research and Development Center, Namyang Dairy Products Corporation)
Publication Information
Korean Journal of Food Science and Technology / v.37, no.6, 2005 , pp. 1024-1027 More about this Journal
Abstract
Rapid and simple method was developed for simultaneous determination of vitamins $D_3\;and\;K_1$ contents in infant formula. Contents of vitamins $D_3\;and\;K_1$, extracted by column-switching HPLC with reversed phase column using enzymatic hydrolysis and organic solvent, in CRM determined by developed method were within certified ranges of standard values.
Keywords
enzymatic hydrolysis extraction; column-switching HPLC; simultaneous determination;
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