• Title/Summary/Keyword: 시들음병

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Development of Efficient Screening Methods for Resistance of Tomato to Fusarium oxysporum f. sp. lycopersici (토마토 시들음병에 대한 효율적인 저항성 검정법 확립)

  • Park, Myung-Soo;Jeong, Bo-Ram;Jang, Kyoung-Soo;Choi, Yong-Ho;Kim, Jin-Cheol;Choi, Gyung-Ja
    • Horticultural Science & Technology
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    • v.30 no.4
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    • pp.426-431
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    • 2012
  • This study was conducted to establish an efficient screening method for resistant tomato to Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (FOL). The resistance degrees of the six commercial cultivars of tomato to the pathogen were evaluated by dipping roots of the seedlings in spore suspension of five FOL isolates. On the basis of the results, two cultivars (Dotaerangmaster, resistant cultivar to FOL race 1; Supersunload, resistant cultivar to FOL race 2) and two isolates (KACC40043, FOL race 2; TF104, FOL race 3) were selected for system establishment. The disease development of the FOL isolates on the cultivars according to several conditions including root wounding, incubation temperature, inoculum concentration and dipping period of roots in spore suspension was investigated. The resistance of each cultivar to the disease was a race-specific response and hardly affected by the tested conditions except for incubation temperature of $20^{\circ}C$. The optimum temperature for disease development caused by FOL was 25 to $30^{\circ}C$. On the basis of the results, we suggest that an efficient screening method for resistant tomato cultivars to Fusarium wilt is to dip the non-cut roots of tomato seedlings at two-leaf stage in spore suspension of $1{\times}10^7\;conidia{\cdot}mL^{-1}$ for 0.5 hours and transplant the seedling to plastic pot with horticulture nursery media, and then to cultivate the plants in a growth room at $25^{\circ}C$ for 3 weeks with 12 hours light a day.

Control of Fusarium Wilt of the Strawberry Caused by Fusarium oxysporum f. sp. fragariae of Solarization with Compost and Calcium Cyanamide Application (태양열 소독시 유기물과 석회질소 병행 처리에 의한 딸기 시들음병균 방제)

  • Nam, Myeong-Hyeon;Kim, Hyeon-Suk;Kim, Hong-Gi
    • Research in Plant Disease
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    • v.17 no.1
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    • pp.32-37
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    • 2011
  • Field experiments were conducted to evaluate the effectiveness of soil solarization at the Nonsan Strawberry Experiment Station, Korea in 2006 and 2008. In in vitro tests, exposure times to achieve an $LD_{100}$ of Fusarium oxysporum f. sp. fragariae were 6.6 days and 5.1 days at $45^{\circ}C$ and $50^{\circ}C$, respectively. A 100% lethal temperature was $46.7{\pm}0.1^{\circ}C$ for the same fungus. For field trials, solarization was conducted during the summer season using polyethylene mulch in a plastic house. The organic matter+calcium cyanamide+solarization treatment increased pH, organic matter, and calcium content compared to those before treatment in soil analysis, but no effect had an urea+solarization treatment. The temperatures at 10 cm depth were different in each treatment and the highest temperature was recorded from July 30 to August 10. The average temperature in organic matter+calcium cyanamide+solarization treatment at 10 cm depth was $3{\sim}4^{\circ}C$ higher than that in all the other treatments. All solarization treatments reduced the soil population of F. oxysporum f. sp. fragariae at 100% in 2008 relative to the non-treated control. All solarization treatments reduced Fusarium wilt incidence to 0% in 2006 and 2008. The effect of organic matter+calcium cyanamide+solarization against F. oxysporum f. sp. fragariae indicates that there may be future alternatives to traditional solarization for disease control as well as reducing the time needed.

Chemical Control of Fusarium Wilt of Strawberry Caused by Fusarium oxysporum f. sp. fragariae (딸기$\cdot$시들음병(Fusarium oxysporum f. sp. fragariae)의 약제방제)

  • Cho Chong Taik;Moon Byung Ju
    • Korean Journal Plant Pathology
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    • v.1 no.1
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    • pp.28-32
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    • 1985
  • The experiments were carried out to investigate a practical method for controlling wilt of strawberry by fungicides in vitro and in field. Captafol and benomyl were the most effective in inhibiting chlamydos-pore germination and mycelial growth of the fungi. In field tests, captafol, benomyl and thiophanate-methyl showed some control effect against the disease. The propagule number of F. oxysporum f. sp. fragariae in soil was greatly reduced by soil drenches of captabol, whereas that of Trichoderma sp. was increased.

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Control Efficacy of Fungicide Injection on Oak Wilt in the Field (살균제 나무주사를 이용한 참나무 시들음병 방제 효과)

  • Son, Su-Yeon;Seo, Sang-Tae;Park, Ji-Hyun
    • Research in Plant Disease
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    • v.20 no.4
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    • pp.295-298
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    • 2014
  • Oak wilt caused by Raffaelea quercus-mongolicae was first noticed in South Korea in 2004 and, ever since, its distribution and damage have been increasing. To screen a fungicide effective for oak wilt control by tree injection, laboratory and field experiments were conducted. Ten fungicides and one antibiotic were examined in vivo for their effectiveness in restricting the growth of R. quercus-mongolicae and R. quercivora (Japanese oak wilt pathogen) isolates. To the Korean isolates of R. quercus-mongolicae, chlorothalonil showed the highest fungicidal effects, followed by benomyl and propiconazole. To the Japanese one, propiconazole was highest in the fungicidal effectiveness, followed by benomyl and bitertanol. Propiconazole was selected for field-testing of its control efficacy because it showed good fungicidal effects in vitro and systemic activity. The control efficacy in the field was 87.5% in the first year of injection and 66.7% in the second year, indicating the fungicidal effects last at least over one year.

Antagonistic Effect of Lactobacillus sp. Strain KLF01 Against Plant Pathogenic Bacteria Ralstonia solanacearum (세균성 시들음병에 대한 식물성 유산균(Lactobacillus sp.)의 저해효과)

  • Shrestha, Anupama;Choi, Kyu-Up;Lim, Chun-Keun;Hur, Jang-Hyun;Cho, Sae-Youll
    • The Korean Journal of Pesticide Science
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    • v.13 no.1
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    • pp.45-53
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    • 2009
  • An antagonistic bacterial strain KLF01 was isolated from rhizosphere of tomato and identified to be Lactobacillus sp. by biochemical and genetic analysis. This strain showed antagonism against the used plant pathogenic bacteria like Ralstonia solanacearum, (bacterial wilt), Xanthomonas axonopodis pv. citri, (Citrus canker), Xanthomonas campestris pv. vesicatoria (Bacterial spot), Eriwinia pyrifoliae (Shoot-blight) and Eriwinia carotovora subsp. carotovora group (Potato scab) through agar well diffusion method. In planta test done by drench application of strain KLF01 $(4{\times}10^8 cfu/ml)$ into the experimental plot containing tomato (Solanum lycopersicum L.) cultivar 'Lokkusanmaru' and red pepper (Capsicum annuum L.) cultivar 'Buja' plants, in pot test post-inoculated with the plant pathogenic bacteria, R. solanacearum significantly reduced the disease severity, compared to the non-treated plants.