• Title/Summary/Keyword: 스폰지법

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Preparation and Characterization of Tissue Engineered Scaffold Using Porcine Small Intestinal Submucosa and Hyaluronic Acid (돼지의 소장점막하 조직과 히알루론산을 이용한 조직공학적 담체의 제조 및 특성분석)

  • Lim, Ji-Ye;Kim, Soon-Hee;Kang, Gil-Son;Rhee, John M.
    • Polymer(Korea)
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    • v.32 no.5
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    • pp.415-420
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    • 2008
  • The porcine small intestinal submucosa (SIS) without immunorejection responses and hyalunonic acid (HA) can be used as biomaterials. In this study, we tried to design and characterize novel sponge. SIS- HA sponge was prepared by freeze-drying after addition 1wt% HA solution into fabricated SIS sponge. Sponge was crosslinked with 1-ethyl-(3-3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC) solution with 100mM concentration for 24 hrs and lyophilized. SIS-HA sponge was characterized by scanning electron microscopy and fourier transform infrared spectrometer. And water absorption ability of sponge was evaluated. We seeded NIH/3T3 cells in SIS-HA sponge and cellular attachment was assayed by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltertazolium-bromide (MTT) test. We demonstrated presence of HA in SIS-HA sponge from C-O functional group observed by the FT-IR analysis. Moreover, we confirmed low cytotoxicity and high cell viability of the SIS-HA sponges. Therefore, we could expect that SIS- HA scaffolds are applicable for the tissue regeneration.

Elliptic Numerical Wave Model Using Generalized Conjugate Gradient Method (GCGM을 이용한 타원형 수치 파랑모형)

  • 윤종태
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.10 no.2
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    • pp.93-99
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    • 1998
  • Parabolic approximation and sponge layer are applied as open boundary condition for elliptic finite difference wave model. Generalized conjugate gradient method is used as a solution procedure. Using parabolic approximation a large part of spurious reflection is removed at the spherical shoal experiment and sponge layer boundary condition needs more than 2 wave lengths of sponge layer to give similar results. Simulating the propagation of waves on a rectangular harbor, it is identified that iterative scheme can be applied easily for the non-rectangular computational region.

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A Finite Element Simulation of Cancellous Bone Remodeling Based on Volumetric Strain (스폰지 뼈의 Remodeling 예측을 위한 체적 변형률을 이용한 유한요소 알고리즘)

  • Kim, Young;Vanderby, Ray
    • Journal of Biomedical Engineering Research
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    • v.21 no.4
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    • pp.373-384
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    • 2000
  • The goal of this paper is to develop a computational method to predict cancellous bone density distributions based upon continuum levels of volumetric strain. Volumetric strain is defined as the summation of normal strains, excluding shear strains, within an elastic range of loadings. Volumetric strain at a particular location in a cancellous structure changes with changes of the boundary conditions (prescribed displacements, tractions, and pressure). This change in the volumetric strain is postulated to predict the adaptive change in the bone apparent density. This bone remodeling theory based on volumetric strain is then used with the finite element method to compute the apparent density distribution for cancellous bone in both lumbar spine and proximal femur using an iterative algorithm, considering the dead zone of strain stimuli. The apparent density distribution of cancellous bone predicted by this method has the same pattern as experimental data reported in the literature (Wolff 1892, Keller et al. 1989, Cody et al. 1992). The resulting bone apparent density distributions predict Young's modulus and strength distributions throughout cancellous bone in agreement with the literature (Keller et al. 1989, Carter and Hayes 1977). The method was convergent and sensitive to changes in boundary conditions. Therefore, the computational algorithm of the present study appears to be a useful approach to predict the apparent density distribution of cancellous bone (i.e. a numerical approximation for Wolff's Law)

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A Study on the Formation Mechanism of Titanium Sponge in the Kroll Process (Kroll법에 의한 타이타늄 스폰지 생성기구에 관한 연구)

  • Jung, Jae-Young;Sohn, Ho-Sang
    • Resources Recycling
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    • v.26 no.5
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    • pp.54-60
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    • 2017
  • In this study, we investigated the effect of $TiCl_4$ injection time on the Kroll reaction at a given weight ratio of $TiCl_4$ and Mg. The reduction reaction was investigated by measuring the temperature change according to $TiCl_4$ injection time and observing the cross section and appearance of the Ti sponge after the reaction. The temperature increment due to Kroll reaction heat generation was found to be linearly proportional to the $TiCl_4$ feed rate. In the graph of $TiCl_4$ injection time and reduction tank temperature, initial temperature peaks were observed irrespective of the injection conditions. This is interpreted to mean a temporary interruption of reaction due to $MgCl_2$ formation after the initial Kroll reaction. In addition, when the cross section of the sponge was observed, a large amount of spherical Mg particles was observed in $MgCl_2$. We can infer that this is the process of continuously feeding the unreacted Mg surface, so that a continuous Kroll reaction takes place. The sponge appearance showed that the coalescence or growth of the Kroll reacted Ti particles can be controlled by the cooling rate.

The Comparison of Sponges and PLGA Scaffolds Impregnated with DBP on Growth Behaviors of Human Intervertebral Disc Cells (DBP 스폰지와 DBP/PLGA 지지체에서의 인간 디스크세포 거동분석 비교)

  • Lee, Seon-Kyoung;Hong, Hee-Kyung;Kim, Su-Jin;Kim, Yong-Ki;Song, Yi-Seul;Ha, Yoon;Lee, Dong-Won;Khang, Gil-Son
    • Polymer(Korea)
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    • v.34 no.5
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    • pp.398-404
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    • 2010
  • We fabricated sponge and poly(lactide-co-glycolide)(PLGA) scaffolds impregnated demineralized bone particle(DBP)(DBP/PLGA) and investigated proper condition to proliferation and phenotype maintenance of intervertebral disc(IVD) cells by comparison between DBP/PLGA scaffold and DBP sponge. DBP/PLGA scaffolds were prepared by solvent casting/salt leaching. Human IVD cells were seeded in scaffolds of two types. Cell viability and proliferation according to scaffolds were analyzed by WST assay and SEM. RT-PCR was assessed to measure mRNA expression of aggrecan and type II collagen of human IVD cells. In WST assay results, cell viability in scaffolds impregnated DBP/PLGA scaffold were higher than DBP sponge. We could observe that disc cell mRNA expressed better in DBP/PLGA scaffold than DBP sponge. We concluded that the using of DBP/PLGA in terms of scaffold fabrication for bio-disc with human IVD cells is helpful growth of disc cells maintenance of phenotypes.

Preparation of Refuse-Derived-Fuel from Automobile Shredder Residues (폐자동차 파쇄 잔류물을 이용한 고분연료의 제조연구)

  • Lee Hwa-Yaung;Oh Jong-Kee;Kim Sung-Gyu
    • Resources Recycling
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    • v.12 no.4
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    • pp.58-64
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    • 2003
  • A study on the preparation of RDF with ASR materials from shredder line of end-of-life vehicles has been peformed by using hot molding method. ASR materials used in the work were plastics, fibers and sponge which were cut by less than 10 mm in size prior to hot molding and 40, 60 and 80 mm mold in diameter were employed for RDF forming. It was found that heating temperature, heating time and mixing ratio of materials played an important role in the hot molding of ASR materials. Density of RDF samples Prepared in the work was in the range of 0.5∼0.9gr/ml depending on the hot molding conditions and it was decreased with increase in the content of sponge. Breaking strength of RDF samples was observed to 0.8∼3.0MPa and it was also increased with increase in density of RDF samples. Finally, when heating temperature was elevated, density of RDF was increased due to Increase in molten part of ASR materials which resulted in the enhancement of breaking strength of RDF.

Residual Stresses and Fracture Toughness in $Al_2O_3$/Al FGMs ($Al_2O_3$/Al 경사기능 재료의 잔류응력 및 파괴인성)

  • 정태주
    • Proceedings of the Korean Powder Metallurgy Institute Conference
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    • 2003.04a
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    • pp.59-59
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    • 2003
  • 경사기능 재료는 위치에 따른 특성 변화를 갖고 있어 변화하는 주변 환경에서 사용되는 재료의 응용성을 높일 수 있는 장점이 있다. 적합한 경사기능 재료의 제조를 위해서는 탄성률, 강도, 인성 등의 위치에 따른 변화를 예측하는 것이 필요하다. 그러나 재료 내에서의 탄성률 변화 등으로 인해 경사기능 재료에서 인성을 정량적으로 표현하는데는 어려움이 있으므로 이를 정량적으로 측정하고, R-curve를 결정하는 것은 재료의 응용성에 중요한 인자가 된다. 본 연구에서는 폴리우레탄 스폰지를 이용하여 밀도의 분포가 연속적으로 다른 폴리 우레탄 스폰지를 제조하고 이에 알루미나 분말 슬러리를 이용하여 slip casting을 행하였다. 그 후 폴리 우레탄 스폰지를 탈지한 후 알루미나 성형체를 소결하여 연속적인 기공률 분포가 다른 다공성 알루미나를 제조하였으며, 이에 Al을 용침하여 $Al_2O_3-Al$ 경사기능 재료를 제조하였다. 이러한 $Al_2O_3-Al$ 경사기능 재료에 대해 파괴인성 및 R-curve 특성을 CT(conpact tension)시편으로 측정하였으며, 이를 균일한 복합체의 파괴인성과 비교하였다. 또한 잔류 응력 특성을 파악하기 위해 실험적이 응력 데이터를 Moire interferometry를 이용하여 결정하였다. 또한 이를 유한요서 해석법(FEM)에 의한 계산치와 비교하였다. 서로 다른 조성 분포를 갖는 $Al_2O_3-Al$ 경사기능 재료와 균일한 복합체의 파괴인성을 비교한 결과 동일한 Al조성에서도 서로 다른 파괴 인성치가 나타났다. $Al_2O_3-Al$ 경사기능 재료에서 파괴인성에 영향을 줄 수 있는 인자로는 술수한 $Al_2O_3$의 파괴인성에 Al금속의 소성변형에 의한 인성증진 효과, 그리고 경사기능 재료에서 상호 조성차이에 따른 잔류응력을 고려할 수 있을 것이다. 이중 $Al_2O_3-Al$ 경사기능 재료의 파괴인성에 미치는 잔류응력의 영향을 고려하기 위해 이의 잔류응력에 대해 실험에 의한 유추된 잔류응력과 FEM계산에 의해 유추된 잔류 응력을 비교, 분석하였다.

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도계육의 시료 채취방법에 따른 세균의 검출량 비교

  • Yun, Hye-Suk
    • Bulletin of Food Technology
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    • v.18 no.1
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    • pp.127-135
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    • 2005
  • 상업적으로 시판되는 10주 미만의 식용 영계육을 처리하는 공정에서 25 그룹의 내장적출하지 않은 것 또는 내장적출한 도계육을 도계공정의 네지점에서 채취하였다. 도계 공정 각 지점의 계육으로부터 무작위로 선택된 부위에서 껍질을 잘라낸 것, 목의 껍질을 잘라낸 것, 셀룰로스 아세테이트 스폰지 재질의 스왑봉으로 계육을 스왑법으로 채취한 것, 또는 도계육을 통째로 세척한 세척액이다. 총균수, 대장균군, 대장균을 각 시료로부터 증균하였다. 사방 1 cm 안의 평균 로그값(log A)과 사방 25cm안에서 측정된 전체 측값치들의 로그값(N)은각 그룹 당 25개였다. 동일한 처리공정 단계에서 얻은 계육으로부터 얻은 같은 그룹의 세균의 측정값의 경우에, 무작위로 선택한 부위에서 잘라낸 껍질 또는 목에서 벗겨낸 껍질, 세척액 샘플에서 측정한 균수의 집합인 N의 세 값 또는 log A의 세 값은 <0.5 logunit 차이였다.그러나 스왑법으로 측정한 사방 1 cm안의 평균로그값(log A)과 사방 25 cm 안에서 측정된 전체측정치의 로그값(N)의 대략 절반이 다른 방법들로 측정한 동일한 측정항목의 값과 >0.5 log unit 까지 차이가 났다. 이런 결과로부터 도계육에서 무작위로 선택한 부위나 목에서 껍질을 잘라내는 방법, 또는 세척액 채취 등의 샘플링 방법은 도계육 표면의 단위영역 당 유사한 양의 세균이 검출될 것이라는 것을 나타낸다.

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