• Title/Summary/Keyword: 수정능력

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Effect of Dimethyl Amiloride on the Acrosome Reaction in Mouse Epididymal Sperm in vitro (생쥐 정자의 첨체반응에 미치는 Dimethyl Amiloride의 영향)

  • 계명찬
    • Development and Reproduction
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    • v.3 no.1
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    • pp.87-93
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    • 1999
  • The possible role of Na$^{+}$/H$^{+}$ antiporter in both the capacitation and the acrosome reaction (AR) was examined in mouse epididymal spermatozoa. Spontaneous acrosome reaction was inhibited by dimethyl amiloride (DMA), a specific inhibitor of Na$^{+}$/H$^{+}$ antiporter, with dose dependent manner. Follicular fluid- or A23l 87-induced acrosome reaction was not inhibited by DMA. It suggests that change in pH$_{i}$ by monovalent cation transport through the Na$^{+}$/H$^{+}$ antiporter is possibly engaged in the capacitation and that agonist- as well as A23l87-induced AR in capacitated sperm might be independent from the Na$^{+}$/H$^{+}$ antiporter. Conclusively, changes in pH$_{i}$ through the Na$^{+}$/H$^{+}$ antiporter might be important for sperm capacitation and it virtually occurs upstream of the $Ca^{2+}$ influx which precedes the acrosome reaction in mouse epididymal spermatozoa.pididymal spermatozoa.

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Spermatogenesis and Fertility Following Orchiopexy and GnRH Treatment in an English Bulldog after Puberty with Bilateral Cryptorchidism (양측성 잠복고환견에서 성성숙 후 고환하강고정술과 GnRH 투여를 통한 생식능력 회복례)

  • Oh, Hong-Geun;Kim, Sue-Hee;Park, Jong-Seong;Kim, Yong-Jun
    • Journal of Veterinary Clinics
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    • v.26 no.6
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    • pp.647-649
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    • 2009
  • An orchiopexy was performed in an 18-month old adult English bulldog with bilateral cryptorchidism. One month postoperatively, the dog was twice-treated with GnRH (50 ug/kg) at an interval of 2 weeks. Semen was collected and evaluated before and after surgery. Fertility was determined by artificial insemination. No spermatozoa were observed before orchiopexy and 2 months postoperatively. However, 6 live sperm were detected 4 months postoperatively and normal sperm characteristics (except sperm concentration) were present 7 months postoperatively. A female bulldog, inseminated with the semen from the bulldog 8 months postoperatively, delivered 6 offsprings. Spermatogenesis and spermatozoa with fertilizing capacity were recovered by postpubertal orchiopexy and GnRH therapy in a bilateral cryptorchid dog.

Developmental Lesions in Amphibian Embryos Induced by Ultraviolet Irradiation of the Fertile Egg (자외선에 의한 양서류 수정난의 발생 결함)

  • Chung, Hae-Moon;George M. Malacinski;Kim, Byeong-Gee
    • The Korean Journal of Zoology
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    • v.20 no.2
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    • pp.109-122
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    • 1977
  • Ultraviolet irradiation of the vegetal hemisphere of the fertilized frog egg prior to first cleavage resulted in alterations in neural morphogenesis. The sensitivity to UV irradiation dropped drastically at the point of 2/3 time lapse between fertilization and the appearance of the first cleavage furrow. Scannining electron micrographs revealed a decrease in size of the cells on the surface of the irradiated embryos. However, ectoderm exchange frafts indicated that the ectoderm of the irradiated embryo retains its capaciiy to form a completely normal neural morphology and epidermal surface. These facts were interpreted in terms of a decrease in the capacity for invagination during gastrulation, and subsequent primary embryonic induction.

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Estimation of Genetic Variations and Selection of Superior Lines from Diallel Crosses in Layer Chicken (산란계종의 잡종강세 이용을 위한 유전학적 기초연구와 우량교배조합 선발에 관한 연구)

  • 오봉국;한재용;손시환;박태진
    • Korean Journal of Poultry Science
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    • v.13 no.1
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    • pp.1-14
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    • 1986
  • The subject of this study was to obtain some genetic information for developing superior layer chickens. Heterosis and combining ability effects were estimated with 5,759 progenies of full diallel crosses of 6 strains in White Leghorn. Fertility, hatchability, brooder-house viability, rearing- house viability, laying-house viability, age at 1st egg laying, body weight at 1st egg laying, average egg weight, hen-day egg production, hen-housed egg production, and feed conversion were investigated and analyzed into heterosis effect, general combining ability, specific combining ability and reciprocal effect by Grilling's model I. The results obtained were summarized as follows; 1. The general performance of each traits was 94.76% in fertility, 74.05% in hatchability, 97.47% in brooder-house viability, 99.72% in rearing-house viability, 93.81% in laying-house viability, 150 day in the age at 1st egg laying, 1,505g in the body weight at 1st egg laying, 60.08g in average egg weight, 77.11% in hen-day egg production, 269.8 eggs in hen-housed egg Production, and 2.44 in feed conversion. 2. The heterosis effects were estimated to -0.66%, 9.58%, 0.26%, 1.83%, -3.87%, 3.63%, 0.96%, 4.23%, 6.4%, and -0.8%, in fertility, hatchability, brooder-house viability, laying-house viability, the age at 1st egg laying, the body weight at 1st egg laying, average egg weight, hen-day egg Production, hen-housed egg production and feed conversion, respectively. 3. The results obtained from analysis of combining ability were as follows ; 1) Estimates of general combining ability, specific combining ability and reciprocal effects were not high in fertility. It was considered that fertility was mainly affected by environmental factors. In the hatchability, the general combining ability was more important than specific combining ability and reciprocal effects, and the superior strains were K and V which the additive genetic effects were very high. 2) In the brooder-house viability and laying-house viability, specific combining ability and reciprocal effects appeared to be important and the combinations of K${\times}$A and A${\times}$K were very superior. 3) In the feed conversion and average egg weight, general combining ability was more important compared with specific combining ability and reciprocal effects. On the basis of combining ability the superior strains were F, K and B in feed conversion, F and B in the average egg weight. 4) General combining ability, specific combining ability and reciprocal effects were important in the age at 1st egg laying and the combination of V ${\times}$F, F${\times}$K and B${\times}$F were very useful on the basis of these effects. In the body weight at 1st egg laying, general combining ability was more important than specific combining ability and reciprocal effects, relatively. The K, F and E strains were recommended to develop the light strain in the body weight at 1st egg laying. 5) General combining ability, specific combining ability and reciprocal effects were important in the hen-day egg production and hen-housed egg production. The combinations of F${\times}$K, A${\times}$K, and K${\times}$A were proper for developing these traits. 4. In general, high general combining ability effects were estimated for hatchability, body weight at 1st egg laying, average egg weight, hen-day egg production, hen-housed egg production, and feed conversion and high specific combining ability effects for brooder-house viability, laying house viability, age at 1st egg laying, hen-day egg production and hen-housed egg production, and high reciprocal effects for the age at 1st egg laying.

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수학적 추론 능력 평가 기준에 관한 연구

  • Jeon, Pyeong-Guk;Kim, Eun-Hui;Kim, Won-Gyeong
    • Communications of Mathematical Education
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    • v.13 no.2
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    • pp.425-455
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    • 2002
  • 본 연구는 수학교육에서 강조되고 있는 수학적 힘의 구성 요소 중의 하나인 수학적 추론 능력에 대한 교사들의 구체적인 이해를 돕고, 문제 해결 과정에서 학생들의 추론 능력을 분석하고 평가하는 데 도움을 주기 위해 문헌 연구 및 학생반응 분석결과에 기초하여 귀납적, 유비적, 연역적 추론능력에 대한 평가기준을 개발하였다. 또한, 개발된 평가기준을 구체적인 문제에 적용하였으며 이를 기초로 문제점을 수정 ${\cdot}$ 보완한 후, 전문가의 타당성 검증과 동일한 학생반응에 대한 채점결과의 일치도를 알아봄으로써 신뢰도 검증을 실시하였다.

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Evaluation of Fertility of Artificial Induced Gynogenetic Diploid Male in Paralichthys olivaceus (유도된 자성발생성 2배체 숫컷 넙치(Parlichthys olivaceus)의 생식 능력 평가)

  • KIM Bong-Seok;MOON Young Bong;JEONG Chang Hwa;KIM Dong Soo;LEE Young-Don
    • Journal of Aquaculture
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    • v.7 no.3
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    • pp.151-158
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    • 1994
  • To evaluate the reproductive ability of gynogenetic diploid male. Paralichthys olivaceus. histological analysis of testis. cytological analysis of spermatozoa and fertilization test with normal aggs were studied and the results are as follow; The gonads of gynogenetic diploid male were histologically normal. and many spermatozoa were observed in their testis. Number of spermatozoa from the control and gynogenetic diploid male were $2.58\times10^9$ and $2.42\times10^9$ cells per 1 ml of milt. respectively (P> 0.05). Amount of milt per kg body weight from the gynogenetic diploid male was significantly higher (P< 0.01) than that from the control male (8.3ml). Size and morphology from the two experimental groups were not different (P>0.05). More than $80\%$ of fertilization rates and hatching rates were observed when the eggs from the control were fertilized with the gynogenetic diploid male sperms.

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Evaluation of Modules of Scientific Gifted Programs (과학영재프로그램의 모듈 평가)

  • Sin, Mi-Yeong;Choe, Seung-Eon
    • 한국지구과학회:학술대회논문집
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    • 2010.04a
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    • pp.68-69
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    • 2010
  • 본 연구의 목적은 서울시 소재 과학영재교육원에서 운영하는 프로그램에서 개발된 일부 모듈들에 구현되어있는 과학탐구과정 및 사고과정의 특징이 과학영재프로그램의 목적에 따른 목표를 성취할 수 있는지를 평가하고, 과학탐구과정이나 사고과정의 각 항목을 구현한 탐구의 실제를 알기 위한 것이다. 또, 본 연구의 결과를 토대로 모듈들에 대해 반성하고 개선점을 찾으려는 것이다. 연구 참여자들은 13명이며 연구 목적을 위해 첫째, 영재교육과정 및 평가에 관한 문헌을 근거로 과학영재프로그램의 목적과 목표를 설정, 둘째, 목적과 목표를 근거로 문헌에 제시된 분석틀들을 수정 개발, 셋째, 참여자들이 각자 모듈을 1개 선정하여 분석하고 이를 수정한 후 다시 분석하여 해석, 넷째, 분석결과의 해석을 토대로 반성을 하였다. 각 단계마다 각자 해결한 내용을 공개하고 모든 참여자들이 공개된 내용에 대해 비판하면서 합의하는 과정을 거쳤다. 합의 도출에 의해 첫째, 과학영재프로그램의 목적은 '탐구적 사고과정을 함양하고 지역사회에 유익한 새로운 지식을 창출한다.', 이에 대한 목표는 '자기 주도적인 탐구 계획 능력을 향상한다.'와 '숙달된 탐구 기능을 사용하여 실제문제를 해결할 수 있다.'로 설정하였다. 둘째, 분석틀은 '과학탐구과정' 과 '사고과정'의 두 범주로 구성하였으며, '과학탐구과정'에 해당하는 항목은 '사전탐구, 탐구방법, 해석적 탐구기술, 의사소통능력, 탐구평가, 과학의 본성'으로, '사고과정'에 해당하는 항목들은 '논리적 사고, 창의적 사고'로 구성하였다. 셋째, 선정된 모듈들을 분석한 결과 모듈들은 대체로 탐구방법과 해석적 탐구기술을 많이 강조하였는데, 수정된 모듈들에서는 이 두 항목이 더욱 강조되었다. 특히, 해석적 탐구기술에서 의사소통능력, 탐구평가, 과학의 본성, 창의적 사고를 강조하는 탐구의 실제가 증가하였다. 수정후 증가된 항목들은 앞에서 설정한 두 가지 목표에 도달하는데 도움이 되는 항목들이라고 합의 선정되었던 것이므로, 이에 따라 수정 전 모듈보다 수정 후 모듈이 보다 더 영재프로그램의 목표에 도달하기에 적합한 것으로 판단하였다. 넷째, 연구결과를 토대로 반성한 점은 '목표' 에 '창의적 사고'와 '과학의 본성'에 대한 강조가 부족하다는 것, '목표'에서 '실제문제를 해결'할 수 있어야 한다는 것에 비해 '모듈'에서 다루는 주제는 '실제문제'가 아니라 학문적인 질문에 치우쳐 있다는 것, '자기 주도적인 탐구 계획 능력'을 향상시키는 탐구의 실제가 대체로 적게 제시되어 있다는 것이다. 이러한 반성을 영재프로그램의 각 모듈에서 모두 구현할 수 있는 것은 아니지만 본 연구에서 설정한 목적과 목표에 따라 프로그램을 개발하는 경우 이러한 반성점을 고려하여 모듈을 개발한다면 영재프로그램 전체적으로 볼 때는 모두 구현되어 있을 것이다. 본 연구는 서울시에 소재하는 과학영재교육원들에서 개발하여 사용하는 많은 모듈 중 극히 일부를 대상으로 하였으므로 연구 결과를 모든 과학영재프로그램에 적용하기에는 한계가 있다. 그러나 본 연구처럼 프로그램이나 모듈을 평가하려는 연구자들에게 일련의 평가단계에 대한 시사점을 제공할 수 있을 것으로 생각한다.

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Analysis of Science Process Skills and Suggestions for Developing Scientific-Inquiry of Secondary Science Gifted Students (과학영재 탐구활동에 제시된 과학탐구능력 분석 및 개발을 위한 제안)

  • Shin, My-Young
    • Journal of Gifted/Talented Education
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    • v.23 no.2
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    • pp.289-310
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    • 2013
  • The purpose of the study is to investigate science process skills and suggest several considerations about developing scientific inquiries for secondary science gifted students. To do this, we analyzed scientific inquiries of science gifted programs and evaluated them on the quantity of problem perception, problem finding and inquiry planning that are regarded as high level science process skills, then revised each inquiry to include those high level skills. The result was that the first, there were differences in frequencies and types of science process skills among those inquiries. The second, there were very few problem perception and problem finding and were not many inquiry planning. The third, some of the revised inquiries showed those high level skills. From this, we would like to suggest we should construct scientific inquiries of science gifted program out of many and various themes. And there should be more high level science process skills such as problem perception, problem finding, and inquiry planning. For this, scientific inquiry developers should have intentions to involve such science process skills which is appropriate for science gifted student.

낙농경영의 승패는 육성우관리가 중요

  • 손민우
    • 월간낙농육우
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    • v.19 no.3 s.203
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    • pp.92-93
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    • 1999
  • 종축개량을 통한 고능력우 위주의 목장경영을 위해 '87년부터 종축개량을 실시하여 현재는 전 두수가 혈통등록이 되어 있고 젖소개량부로부터 검정등록을 실시하면서 검정성적을 기록 관리하며 개체별 관리를 철저히 하고 근친 및 형질개량을 위하여 우수한 고능력우의 정액을 이용하고 '90년도부터는 자가인공수정을 시키고 있다.

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