Spermatogenesis and Fertility Following Orchiopexy and GnRH Treatment in an English Bulldog after Puberty with Bilateral Cryptorchidism

양측성 잠복고환견에서 성성숙 후 고환하강고정술과 GnRH 투여를 통한 생식능력 회복례

  • Oh, Hong-Geun (Wow Veterinary Clinic) ;
  • Kim, Sue-Hee (Laboratory of Veterinary Obstetrics and Theriogenology, College of Veterinary Medicine, Chonbuk National University) ;
  • Park, Jong-Seong (Wow Veterinary Clinic) ;
  • Kim, Yong-Jun (Laboratory of Veterinary Obstetrics and Theriogenology, College of Veterinary Medicine, Chonbuk National University)
  • Published : 2009.12.31

Abstract

An orchiopexy was performed in an 18-month old adult English bulldog with bilateral cryptorchidism. One month postoperatively, the dog was twice-treated with GnRH (50 ug/kg) at an interval of 2 weeks. Semen was collected and evaluated before and after surgery. Fertility was determined by artificial insemination. No spermatozoa were observed before orchiopexy and 2 months postoperatively. However, 6 live sperm were detected 4 months postoperatively and normal sperm characteristics (except sperm concentration) were present 7 months postoperatively. A female bulldog, inseminated with the semen from the bulldog 8 months postoperatively, delivered 6 offsprings. Spermatogenesis and spermatozoa with fertilizing capacity were recovered by postpubertal orchiopexy and GnRH therapy in a bilateral cryptorchid dog.

양측성 잠복고환을 가진 18개월 연령의 잉글리쉬 불독에서 고환하강고정술을 실시하였고 수술 1달 후 GnRH를 투여하였다. 수술 전후 정액검사를 실시하였으며 인공수정을 통해 생식능력을 검증하였다. 수술 전과 수술 2달 후 정액검사 결과, 무정자증으로 판단되었으나 수술 4개월 경과 후 6마리의 정자가 관찰되었다. 수술 7개월 후 정자수를 제외한 나머지 정자 소견에서 정상을 나타내었으며 수술 8개월 후 채취된 정액을 암컷 불독에게 인공수정한 결과 6마리의 건강한 산자를 생산하였다. 양측성 잠복고환견에서 성성숙 이후라 할지라도 고환하강고정술과 GnRH 투여를 통해 정자형성과 수정능력이 회복 가능함을 알 수 있었다.

Keywords

References

  1. Albescu JZ, Bergada C, Cullen M. Male fertility in patients treated for cryptorchidism before puberty. Fertil Steril 1971; 22: 829-833
  2. Hecker W, Hienz HA. Cryptorchidism and fertility. J Pediatr Surg 1967; 2: 513-517 https://doi.org/10.1016/S0022-3468(67)80005-8
  3. Hinman F, Jr. Optimum time for orchiopexy in cryptorchidism. Fertil Steril 1955; 6: 206-214
  4. Humke VR. Treatment results after application ot the LHFSH releasing hormone on maldescensus testis of the male dog. Kleinterpraxis 1977; 22: 315-322
  5. Kawakami E, Naitoh H, Ogasawara M, Tamura M, Hasegawa J, Tsutsui T, Ogasa A. Hyperactivation and acrosome reaction in vitro in spermatozoa ejaculated by cryptorchid dogs after orchiopexy. J Vet Med Sci 1991; 53: 447-450 https://doi.org/10.1292/jvms.53.447
  6. Kawakami E, Tsutsui T, Ogasa A. Peripheral plasma levels of LH, testosterone, and estradiol-17 beta before and after orchiopexy in unilaterally cryptorchid dogs. Nippon Juigaku Zasshi 1990; 52: 179-181 https://doi.org/10.1292/jvms1939.52.179
  7. Kawakami E, Tsutsui T, Yamada Y, Ogasa A, Yamauchi M. Spermatogenic function and fertility in unilateral cryptorchid dogs after orchiopexy and contralateral castration. Nippon Juigaku Zasshi 1988; 50: 754-762 https://doi.org/10.1292/jvms1939.50.754
  8. Kawakami E, Tsutsui T, Yamada Y, Ogasa A, Yamauchi M. Spermatogenic function in cryptorchid dogs after orchiopexy. Nippon Juigaku Zasshi 1988; 50: 227-235 https://doi.org/10.1292/jvms1939.50.227
  9. Kawakami E, Tsutsui T, Yamada Y, Yamauchi M. Cry-ptorchidism in the dog: occurrence of cryptorchidism and semen quality in the cryptorchid dog. Nippon Juigaku Zasshi 1984; 46: 303-308 https://doi.org/10.1292/jvms1939.46.303
  10. Robinson JN, Engle ET. Some observations on the cryptorchid testis. J Urol 1954; 71: 726-734
  11. Romagnoli SE. Canine cryptorchidism. Vet Clin North Am Small Anim Pract 1991; 21: 533-544