• Korean Journal of Animal Reproduction
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• v.15 no.1
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• pp.1-6
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• 1991

This study was carried out to investigate the effects of Ca, BSA, heparin, semen storage and individual bull on motility and acrosome reaction of bovine fresh sperm and sperm stored in lactose-egg yolk solution(LES) at 5$^{\circ}C$ for 4hours, and the results obtained were as follows: 1. When sperm was incubated in SCS containing Ca, BSA, Ca + BSA, heparin, heparin + Ca, heparin + BSA, and heparin + Ca + BSA for 15 minutes, there was significant difference in sperm motility among the treatments, especially BSA showed significantly higher sperm motility than the others. Also there was significant difference in sperm acrosome reaction among the treatments, especially BSA and Ca + BSA showed significantly higher sperm acrosome reaction than the others. 2. Bull KNC 1 showed significantly higher sperm motility than KNC 1, HOL 1 and 2 in both fresh and stored semen, however KNC 1 showed significantly lower sperm acrosome reaction than KNC 1, HOL 1 and 2. Therefore, there was significant difference in sperm motility and acrosome reaction among individual bulls. 3. When KNC 1 and KNC 2 sperm were incubated in SCS and SCS + Ca, SCS + BSA, SCS + Ca + BSA, SCS + heparin, SCS + heparin + Ca, SCS + heparin + BSA, and SCS + heparin + Ca + BSA, there was significant difference in sperm motility among individual bulls, especially BSA in KNC 1 and BSA, Ca and Ca + BSA in KNC 2 showedsignificantly higher motility than the others. However, there was significant difference in sperm acrosome reaction among individual bulls, Ca in KNC 1 and Ca + BSA in KNC 2 showed higher acrosome reaction than the others.

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2002.11a
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• pp.46-50
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• 2002

광양만의 저서환경을 평가하기 위하여 광양만 표층퇴적물에 대한 퇴적학적, 지화학적 특성을 연구하였다. 입도분석결과 표층퇴적물의 입도특성 분포는 사질퇴적물과 점토질 퇴적물이 다양하게 분포하고 있는 것으로 나타났다. 사질을 포함하는 퇴적물은 주로 POSCO 우측해역과 남해도 사이의 수로 중앙부분에 분포하고 있으며, 그외 지역에서는 점토질 퇴적물이 주를 이룬다. 유기물(TOC) 함량은 약 0.2-2.1%의 범위로 분포하고 있으며, 유기물의 C/N비에 근거한다면 육지에서 공급된 유기물과 현장에서 생성된 유기물이 혼합되어 있음을 지시하고 있다. 저질의 건강상태를 검토하기 위해 퇴적물중의 황화수소(H$_2$S)을 측정한 결과 최고 301 ppm에서 최소 0.7 ppm으로 나타났다. 전체적으로는 묘도안 쪽에서 비교적 높은 값을 보이고 있어 일반적으로 오염된 저서환경이라고 할 수 있다. 기존에 조사된 중형 저서생물의 개체수(species abundance)에 대한 공간분포면화를 조사해본 결과 묘도안쪽에서 낮은 개체수(species abundance)와 종수(species richness)를 보이고 있어 황화수소가 높게 나타나는 지역과 대략적으로 일치하고 있음을 보인다. 추후 저서생물의 다양도나, 저서오염지수(Benthic Pollution Index. BPI)등과 퇴적물증의 유기물이나 황화수소값이 어떻게 관련되는지에 대한 자세한 연구를 필요로 한다.

• Journal of the Korean Data and Information Science Society
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• v.27 no.3
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• pp.741-747
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• 2016

The aim of study was to estimate genetic parameter of carcass traits in commercial Hanwoo steer using national animal model for selection of superior bull. Analyzed data (n=5,843) on carcass traits was collected from 107,020 Hanwoo steer. The animal model was used to estimate heritability and genetic correlations. The estimated heritability of carcass traits were 0.19, 0.17, 0.20 and 0.23 for carcass weight, eye muscle area, backfat thickness and marbling score, respectively. The estimated heritability for carcass traits in commercial Hanwoo are low than estimated heritability of national progeny test population for selection of superior bull because breeding environment, genetic performance of cow and feeding day was different. Therefore, we suggests that animal model can include practical genetic variable based on national animal model to improve genetic performance in commercial Hanwoo.

• Journal of Aquaculture
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• v.10 no.1
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• pp.69-76
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• 1997

Chromatium sp., densely populated on the bacterial plate of Lake Kaiike throughout the seasons, possibly fix N2 and concurrently produce H2 N2 Fixation and H2 production by Chromatium sp. were performed under photoautotrophic growth condition, and of which rates were much higher and showed expontial growth phase. Bacterial plater samples from Lake Kaiike collected on July 27, 1994 were used to know the effect of the light or H2S on N2 fixation and H2 productin by the bacteria. At low light intensity (250 lux), low rates of N2 fixation and H2 production were detected after 18 hours. However, high rates of the production were observed under the condition of high light intensity (1000 lux). On the other hand, a very low rate of N2 fixation was observed without an addition of H2S, while the bacterial rapilly increased N2 fixation and H2 production after adding H2S and the highest rate was observed in case of adding 20mg H2S-S/$m\ell$ to the bacterial plates.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2005.05a
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• pp.191-194
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• 2005

본 연구는 polymerase chain reaction(PCR)기법을 이용하여 SRY 및 ZFY의 성 결정 유전자의 특정 염기서열을 포함하는 primer를 이용하여 Y-염색체 특이적인 쇠고기 성판별 기술을 개발하기 위해 수행하였다. 성 결정 유전자의 영역에 특정 염기서열을 포함하는 primer를 설계 합성하고 이들 primer를 이용하여 PCR 증폭을 실시한 다음, 각각의 증폭산물을 1.5% agarose gel에 전기영동 하여 웅성 특이적 DNA band의 증폭여부를 확인하였다. SRY 유전자에서 웅성개체 쇠고기는 1,348 bp 크기의 단편을 가진 DNA band가 검출되었으나, 자성개체의 경우 DNA band가 전혀 검출되지 않은 것을 확인 할 수 있었다. 또한, ZFY 유전자에서 웅성개체의 쇠고기는 979 bp 크기의 단편을 가진 DNA band가 모두 검출되었으나, 자성개체의 쇠고기에서는 역시 DNA band가 전혀 검출되지 않았다. 즉,SRY 및 ZFY 유전자는 모두 수소에서 유래한 쇠고기에서 웅성 특이적인 DNA band가 정확히 검출된 반면 암소에서 유래한 쇠고기에서는 웅성 특이적 DNA band가 전혀 검출되지 않았다. 따라서, 본 연구에서 개발한 SRY 또는 ZFY의 웅성 특이적 성 결정유전자를 이용하는 쇠고기 성 감별기술은 시중에서 유통 판매되고 있는 쇠고기의 암수 성감별을 위한 유용한 DNA marker(DNA 표지인자)로 활용할 수 있을 것이다.

• Korean Journal of Organic Agriculture
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• v.24 no.4
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• pp.931-944
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• 2016

This study was conducted to compare among bull, steer and heifer on growth performance, blood composition and carcass traits according to fattening phase in Hanwoo. 12 month of aged 4 bulls ($339.8{\pm}18.5kg$), 4 steers ($309.8{\pm}13.2kg$), and 3 heifers ($290.0{\pm}16.7kg$) were allocated in the stalls with individual feeding system during 20 months. Average daily gain was not different among the treatment groups during early fattening phase, but higher in the bulls and lower in the heifers after mid-fattening phase (p<0.05). The difference of body size by animal groups appeared from 24 month of age, and wither height and body length of Hanwoo heifer were the smallest (p<0.05) among treatment group. But wither height was not difference among treatment groups at 32 month of age. At 12 month of age, concentrations of serum cholesterol, triglyceride, glucose and total protein were higher in the heifers compared to other groups, and serum GPT (Glutamic pyruvic transaminase) was the highest (p<0.05) in the bulls, and serum GOT (Glutamic oxaloacetic transaminase) was the highest (p<0.05) at late fattening phase in the bulls. Meat yield index was similar between the bulls and the heifers, and the lowest (p<0.05) in the steers. Marbling score was significantly higher (p<0.05) in the steers and the heifers than in the bulls, and meat quality was the best in the steers among treatment groups. The results of this experiment might be used as a basal data on difference between gender in revision of Korean feeding standard for Hanwoo.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.11a
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• pp.903-906
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• 2008

To analyze the growth of SOB (Thiobacillus novellus) and biochemical corrosion of concrete, simulation test method and device were developed. And two types of simulation tests were conducted according to a transplant method and a concentration of H2SO4. As a result, the SOB growth in distinct manners and antibiosis of specimen were observed. In the case of the specimens indirectly transplanted with SOB through culture solution submersion at a hydrogen sulfide level of 120ppm, the rapid activation of SOB and the resulting sulfuric acid production were observed. However, SOB were shown to grow rapidly and then die out in a relative short period of time. Meanwhile, in the case of the specimens directly transplanted with SOB at a hydrogen sulfide level of 50ppm, the long-term growth of SOB was possible, but the production of sulfuric acid by SOB did not progress. In the case of the antibiotic metal-mixed specimens, SOB with destroyed cell membranes and internal organizations were observed.

• KSBB Journal
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• v.18 no.6
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• pp.529-535
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• 2003

We studied degradation effects of hydrophobic substrate such as kerosene and diesel by adding a biosurfactant originated from Pseudomonas aeruginosa F722 and chemical surfactants (Tween 80 and detergent) with aeration. The surface tensions of the biosurfactant, Tween 80 and detergent were 30mN/m, 39mN/m and 31mN/m, respectively. When the concentration of biosurfactant added in C-medium was 0.01 and 0.15%(w/v), the ratios of hydrocarbon degradation were 94.3％ and 94.2% respectively. It was 6.2%(w/v) higher than when the concentrations of added biosurfactant were 0.05, 0.1 and 0.2％. The degradation ratios of the chemical surfactants (Tween 80 and detergent) were 94.5％ and 93.5％ respectively. The effects of the biosurfactant and chemical surfactants were similar on the degradation ratio in mixtures of kerosene and diesel. However, the population of viable p. aeruginosa F722 at the end of the cultivation period was twice as higher in the biosurfactant than that in the chemical surfactant. We also studied the effect of aeration (0.5vvm) on the degradation ratio. The biosurfactant addition experiment was conducted with 0.5vvm air, 35$^{\circ}C$, 150rpm, pH 8.0, 3days, 1.0% (w/v) substrate. When p. aeruginosa F722 and 0.15%(w/v) biosurfactant were added, the degradation ratio of hydrocarbon was 94.8％. Without p. aeruginosa F722, it was 68%. Thus, with aeration, the degradation ratio of hydrocarbon was increased by 26.8％. In addition, the cultivation time was shortened by 1/3. The degradation ratios of hydrocarbon in shaking culture (cultivation time; 3days) and stationary culture (cultivation time; 10days) were 94.8 and 93.7％ respectively. Thus, the addition of biosurfactant and aeration enhanced the degradation of hydrocarbon originated kerosene and diesel.

• ANNALS OF ANIMAL RESOURCE SCIENCES
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• v.29 no.4
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• pp.142-149
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• 2018

In this study, 844 Hanwoo proven bulls in South Korea (called KPN) were classified into 8 groups based on their birth year. Microsatellite (MS) marker information for paternity identification of each individual is provided at the homepage of the National Agricultural Cooperation Federation, Korea (NACF) and is mainly for the analysis of genetic diversity and structural changes. The polymorphism analysis of KPN whole groups revealed the average number of alleles in each marker (number of alleles), the expected heterozygosity ($H_{ex}$), the observed heterozygosity ($H_{ob}$), the polymorphism information content (PIC) and the $F_{is}$ mean as 10.54, 0.764, 0.773, 0.727 and -0.014, respectively. For group D, with the birth year 2004-2005, the $H_{ex}$ and $H_{ob}$ were 0.777 and 0.792 respectively and the PIC was 0.740. The $H_{ex}$ of group C and D, with birth years 2003-2004 and 2007-2008, respectively, were greater than $H_{ob}$. In all the other groups, $H_{ob}$ was greater than $H_{ex}$. Genetic composition and structure were analyzed using STRUCTURE software. According to the analyzed results, the generation of Hanwoo groups showed changes in specific genetic components according to the flow. It was confirmed that the continuous improvement in the Hanwoo affects the genetic structure of the proven bulls group. The results of this study are expected to be used for enhancing the efficiency of Hanwoo improvement project.

• Korean Journal of Ecology and Environment
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• v.41 no.spc
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• pp.86-92
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• 2008

This study was conducted to determine removal effect on phytoplankton (chlorophyll-${\alpha}$ as whole algae) and cyanobacteria by a fish Pseudorasbora parva and macroinvertebrate Palaemon paucidens in September 2006. Three treatments with 25 (T1), 50 (T2) and 100 (T3) individuals along with control (C1, no input fish), and two treatments with 25 (T4) and 50 (T5) individuals along with control (C1) were made for fish and macroinvertebrate, respectively. The initial concentrations of chlorophyll-${\alpha}$ $(Chl_i)$ in each 10L test tank were set up for the levels of $95{\sim}100{\mu}g\;L^{-1}$ and the daily values were monitored in the test tank during 7 days. In the lab tests, P. parva did not show Chl-${alpha}$ removal effect; the removal rate of Chl-${alpha}$ for P. parva was -58% in T1, -56% in T2, and 61% in T3 during the test period. In contrast, P. paucidens. in the treatments of T4 and T5 removed the phytoplankton effectively and the removal effect were appeared to be 33% and 22%, respectively. Also, P. paucidens showed high feeding efficiency in the removal of cyanobacteria. The levels of cyanobacteria were greatly lowed from 6,048 to 927 cells $mL^{-1}$ in T4 and from 6,539 to 1,053 cells $mL^{-1}$ in T5, resulting in 85% and 84% in the removal effect, respectively. Our results for biomanipulation tests suggest that P. paucidens may be used as a potential candidate organism for algae control in spite of the preliminary results by laboratory tests.