• The Korean Journal of Mycology
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• v.46 no.1
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• pp.34-42
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• 2018

Tricholoma matsutake (Pine mushroom) is expensive, and its artificial cultivation has been tried in several countries. Until date, the only successful cultivation of artificial pine mushroom in pine forests uses matsutake-infected pine trees. The National Institute of Forest Science in Korea has been restudying this method since 2000. Success in fruit production and reproduction was achieved in 2010 and 2017, respectively, in the same locale. The successes proved that pine mushrooms could be cultivated artificially in the field using matsutake-infected pine trees. The fruiting of pine mushroom in October 2010 occurred 6 years, 6 months after the transplantation of matsutake-infected pine trees. Five pine mushrooms reoccurred in September 2017, 13 years, 5 months and 15 years, 5 months after the transplantation of the respective matsutake-infected pine trees. The distance between the matsutake-infected pine tree and the pine mushrooms was 12 cm at 6.6 years, 90~115 cm at 13.5 years, and 95 cm at 15.5 years. Fruiting bodies occurred 13~16 days after the underground temperature declined to below $19^{\circ}C$. In conclusion, the use of matsutake-infected pine trees remains the only way to artificially cultivate pine mushrooms. More knowledge of the environmental factors affecting matsutake fruiting would be beneficial.

• The Korean Journal of Food And Nutrition
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• v.23 no.4
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• pp.549-555
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• 2010

This study was conducted to investigate rheological and sensory characteristics of Sulgidduk added with pine mushroom powder. The moisture content of the Sulgidduk ranged from 34.79 to 36.93%. Increasing the amount of pine mushroom powder in the Sulgidduk tended to decrease the pH value, but to increase the $brix^{\circ}$ value. Increasing the amount of pine mushroom powder in the Sulgidduk tended to decrease the lightness(L) in Hunter color value, but to increase the redness(a) and yellowness(b). For the textural characteristics, the addition of pine mushroom powder decreased the adhesiveness and springiness. In sensory evaluation, the addition of 3% pine mushroom powder had the best score in appearance, odor, taste and overall preference. Therefore, this results suggest that adding 3% pine mushroom powder would be feasible for making pine mushroom Sulgidduk.

• 건강소식
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• v.29 no.9 s.322
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• pp.38-39
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• 2005

옛부터 송이가 나는 자리는 자식에게도 가르쳐주지 않는다고 할 정도로 송이는 귀한 음식으로 통했다. '송이'는 소나무 뿌리에서 자라는 버섯이라 해서 붙은 이름. 지표 온도와 일교차, 습도 등 자생조건이 까다로워 인공재배가 불가능한 것으로 알려져 있는데, 현재 우리나라 자연 송이의 산지는 봉화와 양양을 대표로 꼽는다. 특히 봉화 지영의 마사토라는 토질에서 자라난 송이는 향이 진하고 맛이 쫄깃해 전국 최고로 치고 있다. 가을 초반부터 중반까지만 맛볼 수 있어 이 때를 놓치면 1년을 기다려야 한다.

• The Korean Journal of Mycology
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• v.49 no.4
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• pp.469-476
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• 2021

Tricholoma matsutake is commercially important wild edible mushroom. The only method for cultivation of T. matsutake has been to use matsutake-infected pine trees. In this study, twenty-one fruiting bodies were collected from the matsutake-infected pine trees in the Hongcheon experimental site in 2020. The fruiting bodies from the existing production area of matsutake-infected pine tree and from its transplantation site were found to be identical through monitoring and analysis of 13 microsatellite markers. Transplanted matsutake-infected pine trees succeeded in fairy-ring formation and matsutake fruiting in 2010 and 2017 to 2020. In conclusion, the matsutake mycelium of transplanted matsutake-infected pine tree originates from the existing matsutake-infected pine tree production.

• Journal of Korean Society of Forest Science
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• v.102 no.3
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• pp.365-371
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• 2013

Pine mushroom (Tricholoma matsutake) is one of the most profitable forest products in Korea. We postulated a hypothesis that a high rate of returns to labor input could make the harvest of pine mushroom off the optimum level. In the view of developing a sustainable production strategy for pine mushroom producers, production of pine mushroom collectors and pine mushroom growth function were estimated using maximum entropy method. Annual pine mushroom production and labor input were the data used in the estimation of production function of pine mushroom collectors and pine mushroom growth function. The level of sustainable maximum production derived from the estimated function. The production function estimated shows that production of pine mushroom is affected more by the resource of pine mushroom stocked in the forests than by labor that households put in forestry business. The production function of mushroom collectors and the estimated growth function indicate that pine mushroom harvests for the period of 2005-2011 did not reach the potential level of maximum sustainable production. Therefore, we suggest that pine mushroom harvest should be controlled until the resource stock of pine mushroom in the forests increases to the level of maximum sustainable production.

• Journal of Korean Society of Forest Science
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• v.99 no.4
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• pp.479-486
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• 2010

We analyzed the relationships between the daily yield and quality of pine-mushroom to predict the annual production pattern and unit price of the mushroom with the records of pine-mushroom trade at Yeongdeok forestry cooperative's market for nine years (2000~2008). Although there were some exceptions due to extreme drought or extraordinary temperature, the production ratio of high quality (first and second grade) was more than 50% in early stage and decreased, while that of low quality (pileus opened and defected ones) showed increasing pattern after the production reached in peak. The ratio of high quality and that of low quality were reversed 1~9 days before the mushroom production reached the acme of daily yield, which allowed us to predict that the mushroom production would be decreased when the ratio of low quality overcomes that of high quality. The ratio of high quality preceded about 3~4 days prior to that of daily yield, and the mushroom yield showed significant correlations with the ratio of high quality mushroom prior to 3~4 days of the day with the coefficient larger than 0.5 (r=0.51 for 3 days and r=0.54 for 4 days). Thus, we concluded that the analysis of grade distribution of pine-mushroom at the market may provide a significant clue to predict production pattern of the mushroom. In addition, the price of high quality pine-mushroom showed clear negative correlations with the yield. Thus, the analysis may take a good role for the trading of pine-mushroom with providing information for predicting the price of pine-mushroom.

• Journal of Mushroom
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• v.21 no.3
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• pp.190-193
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• 2023

To cultivate pine mushroom (Tricholoma matsutake) artificially, co-cultivation with microorganisms has been introduced. Here, experiments were performed to assess the growth-promoting effect of bacteria on T. matsutake mycelia. Bacteria were isolated from soil samples collected in Yangyang County, Korea. Four of the bacterial isolates (Y22_B06, Y22_B11, Y22_B18, and Y22_B22) exhibited a growth-promoting effect on T. matsutake mycelia (154.67%, 125.91%, 134.06%, and 158.28%, respectively). To analyze the characteristics of the bacteria, especially the antifungal activity, 𝛼-amylase and cellulase activity assays were performed. In comparison with the controls, the isolated bacteria exhibited low 𝛼-amylase and cellulase activity. 16S rRNA gene sequencing was performed to identify the four bacterial isolates. The isolates belonged to the Terrabacteria group and were identified as Microbacterium paraoxydans, Paenibacillus castaneae, Peribacillus frigoritolerans, and P. butanolivorans. These bacterial isolates are expected to have contributed to the growth promotion of T. matsutake mycelia and the artificial cultivation of T. matsutake.

• The Korean Journal of Mycology
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• v.45 no.3
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• pp.188-195
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• 2017

Despite the high commercial value of the pine mushroom (Tricholoma matsutake) around the world, its production still depends upon natural harvesting. In recent years, mushroom researchers in Korea and Japan have been successful in artificially cultivating T. matsutake by producing single mushroom through matsutake-infected pine seedlings. In this study, we determined the minimum seedling height required for the survival of matsutake mycelia in the infected pine seedlings in the field. The survival rate of matsutake mycelia in the matsutake-infected pine seedlings was 50% (14) in two years and 71% (10) in one year after their transplantation. The average height at time of transplantation of the matsutake-infected pine seedlings that bore surviving mycelia after transplantation was 25 cm (minimum 12 cm to maximum 40 cm). In conclusion, the matsutake mycelium of matsutake-infected pine seedlings was able to survive in field conditions if the height of the seedling at the time of T. matsutake infection was at least 12 cm. These results suggest that the height of the host plants used in conventional matsutake-infected pine seedling production should be greatly reduced to improve the matsutake cultivation. Therefore, standardization of the seedling height for artificial cultivation of pine mushrooms by the matsutake-infected pine seedling method is suggested.

• Journal of Korean Society of Forest Science
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• v.95 no.6
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• pp.636-642
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• 2006

To make a new fairy ring of Tricholoma matsutake in situ, the way of production and transplanting of ectomycorrhizal seedlings of T. matsutake using Pinus densiflora was investigated after transplanting from 2000 to 2005 as well as the method to improve their survival rate for the fungus. For the production of ectomycorrhizal pine seedlings, the seedlings planted at the edge of fairy ring of T. matsutake in November showed 97% of the survival rate, while those planted in April showed 80% of the rate. For the complete infection of the T. matsutake, it required more than two years after planting. The infection rate of mycelia for the ectomycorrhizal seedlings was 17.6% when the natural seedlings were used, whereas it was relatively low when the seedlings prepared from the nursery were used. The survival of T. matsutake mycelium reached up to 22% by the transplanting in April, while the mycelium transplanted in October and November showed less than 5% of the survival. The survival of T. matsutake on the transplanted seedlings was the highest in the seedlings having 50% of infection rate before transplanting. Excavation of the ectomycorrhizal seedling to examine the vitality of ectomycorrhizal roots of T. matsutake resulted in the perishing of them. Therefore, the method using a 'rice bag triers' to check living mycelium of T. matsutake without digging of transplanted seedlings was introduced in this study. In addition, it is recommended that the examination has to be conducted at least two years after transplanting.

• Journal of Mushroom
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• v.22 no.1
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• pp.22-26
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• 2024

Tricholoma matsutake is a traditional favorite food in East Asia, cultivated in fairy rings called "shiro," which are found near Pinus densiflora. For effective artificial cultivation of Tri. matsutake, microorganisms from symbiotic fairy rings are co-cultivated. In this study, one bacterial isolate (Y22_B35) and two fungal isolates (Y22_F64 and Y22_F68) displayed growth-promoting effects on Tri. matsutake mycelium (158.47, 125.00, and 122.26% enhanced growth, respectively). For identification, 16S rRNA or ITS regions from the microorganisms¡¯ genomes were sequenced. Other sequences, including BenA, CaM, and RPB2 were sequenced in the fungal isolates. The bacterial isolate Y22_B35 was identified as Bacillus cereus. Y22_F64 and Y22_F68 were identified as Umbelopsis nana and Aspergillus parvulus, respectively. To identify the effects of the dominant microorganisms on Tri. Matsutake cultivation, metagenomic analyses were performed. Discovery of these Tri. matsutake mycelium growth-promoting microorganisms and metagenomics analyses are expected to contribute to our understanding of Tri. matsutake fruiting body growth and construction of biomimicry.