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Scale Insects (Sternorrhyncha) Occurring on Flowering Plants in Korea (국내 화훼류에 발생하는 깍지벌레(진딧물아목)의 종류)

  • Kwon Gi-Myon;Han Man-Jong;Choi Dong-Ro
    • Korean journal of applied entomology
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    • v.44 no.1 s.138
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    • pp.51-59
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    • 2005
  • A total of 45 scale insects belonging to 29 genera of five families were recognized on 15 kind of flowering plants on the basis of specimens collected in 2003 and specimens deposited in the Insect Collection of NIAST in Korea. Three species were recognized on Korean forsythia, four on chrysanthemum, 15 on common camellia, seven on kobus magnolia, three on rose of sharon, one on Indian lilac, six on rose, seven on rhododendrons, two on bamboo palm, three on benjamin tree, 12 on evergreen euonymus, five on Japanese yew, two on corn plant, six on orchids and two on cacti. Pulvinaria floccifera (Westwood) and Pseudaonidia paeoniae (Cockerell) on common camellia and Pulvinaria citricolar Kuwana on rose of sharon are reported for the first time from the Korean Peninsula.

A Study on the Nucleotide Analysis of 18S rRNA and the Molecular Evolution of the Korean Decapods(II) (한국산 십각류의 18S 리보솜 RNA의 염기분석과 분자진화에 관한 연구(II))

  • Kim, Won;Min, Gi-Sik;Kim, Sang-Hee
    • Animal Systematics, Evolution and Diversity
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    • no.nspc3
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    • pp.139-146
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    • 1992
  • The primary sequence of the 18S rRNA gene of a crustacean Pugettia quadridens (Decapoda: Pleocyemata: Brachyura) was determined by the PCR cloning and Taq sequencing. The 18S rRNA gene of this species in 1837 bases long, and 46 bases shorter than that of another crustacean decapod Oedignathus inermis. The similarity between two species is 90.8% when the insertion and/or deletion sites were excluded. Within the molecule, the most conservative (identical) region locates at the position of 1137-1206 and it is 70 bases long. The most long consecutive nucleotide differences occur at the position between 46-55 and the second most between 399-407. The sequence variation in the primary structure of 18S rRNA gene are not evenly distributed throughout the molecule.

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Population Genetic Structure of Potentilla discolor Bunge, Rosaceae in Korea (한국내 솜양지꽃의 집단 유전 구조)

  • Huh, Man-Kyu
    • Journal of Life Science
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    • v.16 no.6
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    • pp.898-903
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    • 2006
  • The genetic diversity and population structure of fifteen Potentilla discolor Bunge populations in Korea were determined using genetic variations at 19 allozyme loci. Fourteen of the 19 loci (73.7%) showed detectable polymorphism. Genetic diversity at the species level and at the population level was high ($H_{ES}\;=\;0.215$, $H_{EP}\;=\;0.196$, respectively), whereas the extent of the population divergence was relatively low $(G_{ST}\;=\;0.069)$. Total genetic diversity values $(H_T)$ varied between 0.0 and 0.656, giving an average overall polymorphic loci of 0.292. The interlocus variation of genetic diversity within populations $(H_S)$ was high (0.274). On a per locus basis, the proportion of total genetic variation due to differences among populations $(G_{ST})$ ranged from 0.010 for Pgm-2 to 0.261 for Pgd-2 with a mean of 0.069, indicating that about 6.9% of the total allozyme variation was among populations. Wide geographic ranges, perennial herbaceous nature and the persistence of multiple generations are associated with the high level of genetic variation in P. discolor. The estimate of gene flow based on $G_{ST}$ was high among Korean populations of P. discolor (Nm = 3.36).

리보솜 불활성화 단백질의 삼차원 구조 규명과 그 구조의 신약개발에의 응용

  • 서세원
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1994.04a
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    • pp.264-264
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    • 1994
  • 본 연구는 보리 씨앗에 존재하는 리보솜 불활성화 단백질(RIP) 의 삼차원 구조를 X-선 결정학 방법을 이용하여 밝히고, 그 결과로 분자 차원에서 기능을 이해하는 것을 목적으로 하고있다. 리보솜불활성화 단백질은 N-glucosidase 반응을 통하여 단백질 합성을 저해하기 때문에 세포를 죽일 수 있다. 따라서 암세포만을 특정적으로 인식하는 다른 물질과 결합시키면 암세포만을 특정적으로 죽일 수 있는 면역독소로 이용될 수 있다. 또, 최근에는 항바이러스의 작용을 함이 밝혀져 많은 연구가 진행되고 있다. 단백질 삼차원 구조 규명을 위해서는 여러가지 단계가 있는데 지난 번 과제까지 성공적으로 리보솜 불활성화 단백질의 대량 분리와 X-선 결정학의 필수 요건 좋은 결정을 길렀고, 이번에는 구조 해석을 위해 꼭 해결해야하는 위상문제를 극복하기 위하여 여러가지 실험을 진행하였다. 우선, 비슷한 구조인 피마자씨에서 분리한 Ricin의 A-체인과 미국자리공 잎에서 분리한 Pokeweed antiviral protein의 삼차원 분자좌표를 이용하여 분자치환법으로 위상문제 해결을 시도하였다. Ricin 의 A-체인을 이용하였을 때 분자의 위치가 정확히 찾아지지 않았고, 다른 모델인 Pokeweed antiviral protein을 이용하여 X-PLOR 프로그램내의 PC refinement법으로 분자치환을 시도하였다. Euler각도로 (187.37, 22.5, 311.94) 의 회전해 (Rotation solution) 를 가지고 있었고, 이러한 해에 맞추어서 분자를 돌려둔 후, 이동해 (Transaltion solution) 을 구해서 그 위치 (Orientation) 로 분자를 이동하였다. 이 때 R값은 53.9 % (8.0 - 3.5$\AA$) 이였고, 부분좌표 (Fractional coorcdinate) 에서는 0.102, 0.000, 0.261 이고, 직교좌표 (Orthogonal coorclinate) 에서는 4.616, 0.000, 13.167 의 결과를 얻었다.

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A Study on The Antimicrobial Effect of Organic Silicon Quaternary Ammonium Salt Using Cotton, Polyester, and Wool (이불솜의 종류에 대한 유기실리콘 제4급 암모늄염의 항미생물성 효과 -목화솜, 폴리에스테르솜, 양모솜-)

  • 이은영
    • Journal of the Korean Home Economics Association
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    • v.33 no.3
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    • pp.243-253
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    • 1995
  • This study has been carried out for the antimicrobial effects of organic silicon quaternary ammonium salt with which cotton, polyester, and wool were treated respectively, using Esherichia coli and Proteus bulgaris which are experimental bacteria for clothing materials. As a results, the best antimicrobial effects of organic silicon quaternary ammonium salt came out from cotton ; the next form wool ; and lower from polyester. With the changes of the temperature, the antimicrobial effect with soaking time, there was no changes with cotton after 10 minutes passed. It seemed to have reacted entirely in the early stage. The longer the soaking duration was, the higher the effect from polyester was. The effect from wool was increased until 20 minutes, but decreased after 30 minutes. The optimal processing condition of cotton was in the condition of liquor ratio 40:1, concentration 0.5%, soaking time 5 minutes, and temperature 3$0^{\circ}C$ ; wool was 1.5%, 20 minutes, and 6$0^{\circ}C$ ; polyester was 2.0%, 30 minutes, and 3$0^{\circ}C$ respectively. The changes of the effect by washing was as followings : The processing effect on cotton and wool appeared to be everlasting, since they had no changes by washing 10 times ; while, it was remarkablely decreased with polyester by washing only once, and was almost disappeared after washing 10 times, which means that polyester has no durability to washing.

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Development for manufacture of Agaricus bisporus compost by cotton wastes media (방울솜을 이용한 양송이 퇴비배지 제조법 개발)

  • Chang, Hyun-You;Choi, Hyeong-Ki
    • Journal of Mushroom
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    • v.2 no.1
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    • pp.28-32
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    • 2004
  • This experiments was conducted to study on the substitute cotton wastes for ricestraw compost media on cultivation of Agaricus bisporus. This results were as follows. The results of yield according to the mixture rate of cotton wastes($4000{\sim}5500kg/198m^2$), rice hull(4.5%), chicken manure(50%), gypsume(1.5%), urea(2%), tobacco powder((2.5%), seasame meal(5%) and $MgSo_4$(5%) was effected to 57.2% increasement with 64.33kg in summer season, 65.21kg in winter season, 39.6kg in control, respectively. The results of yield according to the mixture rate of cotton wastes($4000{\sim}5500kg/198m^2$), cotton hull(1.25%), rice hull(4.5%), chicken manure(50%), gypsume(1.5%), urea(2%), cigarette powder(2.5%), seasame dregs(5%) and $MgSo_4$(5%) was effected to 62.3% increasement with 61.0kg in summer season, 62.3kg in winter season, 39.6kg in control, respectively. The results of yield according to the mixture rate of cotton wastes($2000{\sim}2500kg/198m^2$), cotton hull(($2000{\sim}2500kg/198m^2$), rice hull(2.5%), chicken manure(37.5%), gypsume(1.5%), urine(2%), cigarette powder((1.25%), seasame dregs(2.5%) and $MgSo_4$(3%) was effected to 40.4% increasement with 57.33kg in summer season, 58.34kg in winter season, 39.6kg in control, respectively.

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Growth and Microsomal ATPase Activity of Lettuce(Lactuca sativa. L.) Cultured in the $KNO_3-Added$ Nutrient Solution (($KNO_3$를 첨가한 양액에서 상추의 생육 및 마이크로솜 ATPase 활성 변화)

  • Lee, Gyeong-Ja;Kang, Bo-Goo;Kim, Hyun-Ju;Min, Kyeong-Beom;Kim, Young-Kee
    • Korean Journal of Environmental Agriculture
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    • v.20 no.1
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    • pp.28-33
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    • 2001
  • Lettuces were grown hydroponically in three different nutrient solutions, normal and 30 or 50 mM $KNO_3-added$ nutrient solutions, and the electrical conductivities of the nutrient solutions were 1.0, 4.5, and 6.5 dS/m, respectively. Lettuces grown in the $KNO_3-added$ nutrient solutions showed a decrease in the germination ratio and the lower indices of growth, such as plant height, stem diameter, leaf length, and leaf width. Microsomes were prepared from the roots of lettuce and characteristics of microsomal ATPases were investigated. The activities of microsomal ATPases grown in the 30 mM and 50 mM $KNO_3-added$ nutrient solutions were higher than that grown in the normal nutrient solution. The highest activities of microsomal ATPases were observed at pH 7.0 in all culture conditions. The activities of microsomal ATPases were increased in a reaction buffer solution containing high concentration of $K^+$, whereas they were decreased in a reaction buffer containing $Na^+$. The stimulating effect of $K^+$ in the reaction buffer was greater on the microsomal ATPases of lettuces grown in the $KNO_3-added$ nutrient solutions than that grown in the normal nutrient solution. These results imply that the activities of microsomal ATPases in the root tissue are increased as increasing the $KNO_3$ concentration in the hydroponical nutrient solution.

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