• Title/Summary/Keyword: 속새

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HPLC Fractionation of Antioxidant Substances of E. hyemale Extract and Analysis of Indicator Components Using LC-MS (속새(Equisetum hyemale) 추출물의 항산화 물질의 HPLC 분획과 LC-MS를 이용한 지표성분 분석)

  • Song, Jin Hwa;Lee, Geo Lyong
    • Journal of Naturopathy
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    • v.10 no.2
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    • pp.108-113
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    • 2021
  • Background: Results on the action of antioxidants in extracts of Equisetum hyemale stems and roots have already been reported, but the antioxidant properties have not been analyzed. Purpose: This study was to determine the molecular structure of antioxidants in substances extracted from E. hyemale stems and roots. Methods: Component analysis was analyzed by HPLC and LC-MS after extraction with hot water and ethyl alcohol. Results: The HPLC chromatogram of stem and root extracts showed four significant peaks at a wavelength of 205 nm. Peak 1 at 280 nm is a typical simple phenolic type, and both peaks 2-4 near 280 nm and 370 nm are typical flavonoid glycosides. As for the antioxidant level of the extract by HPLC analysis, the sum of the peaks at 740 nm was the highest at 3,669 mAU in the 100% ethanol extract, 3,096 mAU in the 70% ethanol extract, and 2,868 mAU in the hot water extract. As a result of LC-MS analysis of the antioxidant extract, kaempferol-3-sophoroside-7-glucoside with a molecular weight of 772 da at peak 3, and kaempferol-3-sophoroside-8-glucoside with a molecular weight of 788 and 772 at peak 4 was identified. Conclusions: The above results show that two types of antioxidants were identified in the antioxidant extract of E. hyemale exrtracts. Therefore, the potential as a raw material for functional cosmetics has increased.

Antioxidant and Elastase Inhibitory Effects of Equisetum hyemale Extract (속새(Equisetum hyemale) 추출물의 항산화 및 Elastase 저해 효과)

  • Song, Jin Hwa;Song, Hyun Sook;Lee, Geo Lyong
    • Journal of Naturopathy
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    • v.10 no.2
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    • pp.86-92
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    • 2021
  • Background: The presence of antioxidants was not confirmed in the medicinal plant Equisetum hyamale grass. Purposes: This study was to determine the antioxidant and elastase inhibition effects of extracts of E. hyemale. Methods: Antioxidant functions of E. hyemale stems and roots were measured and extracted with hot water (HW) and ethyl alcohol (70EOH, 100EOH). Results: The extraction yield of stems was higher in HW extraction than in ethyl alcohol extraction. The polyphenol content was significantly higher in the root extract than in the stem. Total flavonoid content of 70EOH extract was significantly higher in root extract than in stem. The elastase inhibitory function of the extract was 46% in the root and 49% in the stem at 100 ppm of the extract. The ABTS free radical scavenging function was in the order of HW<70EOH<100EOH

Comparison of Flooding Tolerance for the Selection of Plants in Vegetation-Based Low Impact Development Facilities (식생기반형 LID 시설의 식재식물 선정을 위한 내침수성 비교)

  • Lee, Eun-Yeob;Hyun, Kyoung-Hak;Hou, Jin-Sung;Park, Mi-Ok;Koo, Bon-Hak
    • Journal of Environmental Impact Assessment
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    • v.23 no.6
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    • pp.466-476
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    • 2014
  • In this study, 10 species of plants with high adaptability to aquatic environments were compared for selecting plant species suitable for vegetation-based low impact development (LID) facilities. The flooding tolerances of the plants were tested by analyzing their growth status under half-immersion and full-immersion conditions, with varying durations of immersion. In decreasing order of flooding tolerance, the comparative analysis of plant height and leaf width is as follows: Hemerocallis fulva, Juncus effusus var. decipiens, Iris pseudoacorus, Phragmites communis TRIN, Typha orientalis C.Presl, Aster koraiensis Nakai, Iris sanguinea, Equisetum hyemale, Acorus calamus. Specifically, Hemerocallis fulva, Juncus effusus var. decipiens. Iris pseudoacorus showed excellent growth status under both immersion conditions. Iris sanguinea and Equisetum hyemale. withered to death by around day 27 of the experiment, but their flooding tolerance was confirmed to be relatively high. Iris pseudoacoru showed flooding tolerance under the half-immersion condition unlike under the full-immersion condition, when compared on day 21 of the experiment. Aster koraiensis Nakai also thrived better under the half-immersion condition, proving to be a highly immersion-resistant species. On the basis of the results of this experiment, Hemerocallis fulva, Juncus effusus var. decipiens and Iris pseudoacorus, Typha orientalis C.Presl were selected as species suitable for vegetation-based LID facilities.

Lavicidal and Antifeeding Activities of Oriental Medicinal Plant Extracts against Plutella xylostella( Lepidoptera: Yponomeutoidae) and Spodoptera liture (Lepidoptera: Noctuidae) (배추좀나방과 담배거세미나방 유충에 대한 한방식물체의 살충활성 및 섭식 저해활성)

  • 권정현;안용준;권형욱;장경수;조광연
    • Korean journal of applied entomology
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    • v.33 no.4
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    • pp.225-229
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    • 1994
  • The methanol extracts from 30 species of oriental medicinal plants belonging to 24 families were tested for their lhicidal and antifeeding activit~es against diamondback moth (Plutello xylostella L) and tobacco cutworn (Spodoptera litura F.) by a leaf-dipping method at a concentration of 5, 000 ppm. The methanol extract from Copti chinensis only showed a potent larvicidal activity against P xylostello. Strong antifeeding activity against P. xylostello was observed from the extmds from Platycodon grandiflorurn, Codonopsis pilosula, Asomm sieboldii, Rhus chinensis and Uthospermum erythrorhizon And a potent antifeeding activity against S. liturn was obtained from Akebia quinata and Equlsetum hyemale extracts. A significant antifeeding activity against both species was obtained from R chinensis and C. chinensis extracts.

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Distribution and Characteristics of Plant Resources in Mt. Heungjeong (Pyeongchang-gun, Gangwon-do) (흥정산(강원, 평창) 식물자원의 분포와 특징)

  • Han, Jun-Soo;Cheon, Kyeong-Sik;Kim, Kyung-Ah;Yoo, Ki-Oug
    • Korean Journal of Plant Resources
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    • v.25 no.4
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    • pp.416-432
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    • 2012
  • This study was carried out to investigate the distribution and characteristics of plant resources in Mt. Heungjeong (Pyeongchang-gun, Gangwon-do) from April, 2009 to May, 2011. The vascular plants based on voucher specimens were summarized as 403 taxa, including 78 families, 238 genera, 343 species, 3 subspecies, 47 varieties, and 10 forms. Among the investigated 403 taxa, 8 Korean endemic, 1 endangered, and 16 rare plants were included. 71 species of specially designated plant by the Ministry of Environment were also investigated. The naturalized plant were 20 taxa, and the percent of naturalized plant species was estimated as 5.0%. The usefulness of the plants of the 403 taxa listed consists of 175 taxa (43.4%) of edible plants, 126 taxa (31.3%) of medicinal plants, 65 taxa (16.1%) of pasture plants, 52 taxa (12.9%) of ornamental plants, 12 taxa (3.0%) of timber and fiber plants, 3 taxa (0.7%) of industrial plants.

Effects of Aqueous Medicinal Herb Extracts and Aqueous Fermented Extracts on Alcohol-Metabolizing Enzyme Activities (약용식물의 열수추출물과 적정 조성추출물 및 그 발효물이 알콜대사 효소활성에 미치는 영향)

  • Lee, Ka-Soon;Kim, Gwan-Hou;Seong, Bong-Jae;Kim, Hyun-Ho;Kim, Mi-Yeon;Kim, Mee-Ree
    • Food Science and Preservation
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    • v.16 no.2
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    • pp.259-265
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    • 2009
  • To develop an effective anti-hangover product, hot-water extracts of 25 medicinal herbs were screened for inhibition or activation of alcohol dehydrogenase(ADH) and acetaldehyde dehydrogenase(ALDH), and 12 herbs were selected for further study. Chosen medicinal herb extracts(CMHEs) were fermented by Lactobacillus delbruechii subspecies lactis for 10 days at $35^{\circ}C$ after saccharification with nuruk(malt inoculated by 5 types of microbs) for 72 hours at $35^{\circ}C$ and both CMHEs and fermented CMHEs(FCMHEs) were explored for anti-hangover effects in vitro. We found significant ADH inhibition by hot-water extracts of Pueraria thunbergiana, Hovenia dulcis Thunb, Lycium chinense, Glycyrrhiza uralensis, Acanthopanax sessiliflorus, Liriope platyphylla, and Ixeris dentata, and significant ALDH activation by extracts of Acanthopanax sessiliflorus, Lycium chinense, Ixeris dentata, and Polypori umbellati of the Polyporaceae. The ADH effects on CMHE and FCMHE were -20.22% and -62.63% of control values, and the ALDH effects 173.20% and 280.17%, respectively. In rats given 20%(v/v) alcohol(15 mL/kg), FCMHEs significantly decreased blood acetaldehyde concentrations on 3 hours after ethanol administration, in a dose-dependent manner(p<0.05). Notably, blood acetaldehyde concentrations were markedly reduced in animals given FCMHEs(400 mg/kg) compared to levels seen in rats receiving CADB(commercial alcohol detoxification beverage). Thus, anti-hangover effects were promoted by fermentation of certain medicinal herb extracts.

Screening of Inhibitory activities on Angiotensin Converting Enzyme from Medicinal plants (약용식물의 Angiotensin Converting Enzyme 저해활성 탐색)

  • Choi, Geun-Pyo;Chung, Byung-Hee;Lee, Dong-Il;Lee, Hyeon-Yong;Lee, Jin-Ha;Kim, Jong-Dai
    • Korean Journal of Medicinal Crop Science
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    • v.10 no.5
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    • pp.399-402
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    • 2002
  • Angiotensin converting enzyme(ACE) belongs to the class of zinc protease and plays an important role in the regulation of blood pressure. In this experiment, we investigated the inhibitory activities of medicinal plant extracts on ACE. Fifty medicinal plants were selected and the extracts were prepared by refluxing with 70% methanol. Among the extracts, eleven medicinal plant extracts such as Sedum sarmentosum Bunge, Petasites japonicus(s.et z.) Max, Rubus coreanus, Morus bombycis Koidz, Acorus calamus var. angustatus, Glycyrhiza glabra, Equisetum hyemale, Portulaca oleracea L., Prunella vulgaris var. lilacina Nakai, Sorbus commixta Hedl, Allium thunbergii showed more than 50% inhibitory activities, and Paeonia suffruticosa Andr., lnula helenium, Acanthopanax senticosus Harms, Dendrobium moniliforme, Juglans mandshurica, Zizyphus jujuba, Leonurus artemisia, Aster scaber Thunb, Vitex rotundifolia, Platycodon grandiflorum, Prunus persica, Ligularia fischeri showed $40{\sim}49%$ inhibitory activities. Therefore these extracts which contain high ACE inhibitory activities may be useful as antihypertension agents and to the treatment of hypertension.

Survey on Heavy Metals Contents in Native Plant near Old Zinc - Mining Sites (아연광산 인근지역 야생식물중의 중금속 함량 조사)

  • Jung, Ki-Chai;Kim, Bok-Jin;Han, Sang-Guk
    • Korean Journal of Environmental Agriculture
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    • v.12 no.2
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    • pp.105-111
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    • 1993
  • This study was carried out to find heavy metal contents in soil and in native plant in the regions near by zinc-mines, located at the Chilgok and Uljin Gun in Kyeongpook area. In the heavy metal contents during the to growth of native plants, Cd was contained in the order perennial > biennial > annual plant, but Cu was annual > perennial > biennial plant, and there was no difference in Zn contents. The native plants contained heavy metal highest were Osmunda japonica in Cd, Persicaria thunbergii H.G in Cu and Equisetum arvense L. in Zn. Cd was contained highest in Pteridaceae, Cu in Equisetaceae and Zn in Polygonaceae. In the heavy metal contents by the part of plant of Equisetum arvense L. and Erigeron canadensis, Cd and Zn were much contained in the order leaf > stem > root, but Cu was in the order root > leaf > stem. The average contents of Cd, Cu, Zn in soil were 1.27ppm. 12.04ppm. 64.28ppm in Chilgok, and 3.30ppm. 72.93ppm. 194.04ppm in Uljin respectively. There were positive correlations between Cd contents of heavy metals(Cd, Cu, Zn) in soil and in native plant, but not significant. It was estimated that Osmunda japonica, Pteridium aquilinum (KUHN) var and Equisetum arvense L. which most absorbed heavy metal have effect of exclusion of heavy metals near by zine-mines region.

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The Vascular Plants in Mt. Daedun Provincial Park Area, Korea (대둔산 도립공원 일대의 관속식물상)

  • Park, Jin-Sun;Kim, Sang-Jun;Jung, Su-Young;Hwang, Hee-Suk;Bak, Gi-Ppeum;Shin, Hyun-Tak;Yoon, Jung Won;Lee, Jun-Woo;Heo, Tae-Im;An, Jong-Bin
    • Korean Journal of Environment and Ecology
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    • v.32 no.5
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    • pp.445-468
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    • 2018
  • The purpose of this study was to investigate the distribution of vascular plants growing at the Mt. Daedun provincial park area in the boundary between Nonsan-si, Geumsan-gun, Chungcheongnam-do and Wanju-gun, Jeollabuk-do, Korea. We collected the vascular plants 9 times from May 2016 to July 2017. The flora of Mt. Daedun was classified as a total of 434 taxa comprising of 95 families, 278 genera, 388 species, 3 subspecies, 38 varieties and 5 forma. Among them, the endemic plants in Korea that the investigation found were 11 taxa including Broussonetia kazinoki Siebold, Clematis trichotoma Nakai, Thalictrum actaefolium var. brevistylum Nakai, etc. It also found rare plant species, as designated by the Korea Forest Service were 6 taxa including 5 grade LC taxa such as Eranthis stellata Maxim., Viola albida Palib., and Scopolia japonica Maxim. and 1 grade DD taxa, Scrophularia koraiensis Nakai. The special floristic plants were a total of 35 taxa such as 9 taxa in degree III (Eranthis stellata Maxim., Stewartia pseudocamellia Maxim., Acer palmatum Thunb., etc.), 7 taxa in degree II (Equisetum hyemale L., Potentilla dickinsii Franch.&Sav., Viola orientalis (Maxim.) W. Becker, etc.) and 19 taxa in degree I (Dryopteris crassirhizoma Nakai, Cephalotaxus koreana Nakai, Ulmus parvifolia Jacq., etc.) to provide basic data. No taxa in grade V or IV were found. The naturalized plants were 34 taxa including Rumex acetosella L., Phytolacca americana L. and Chenopodium album L., and the percentage of urbanization index was 10.6% while the naturalized plant index was 7.7%.