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Sugar contents of cellulosic hydrolysates according to pre-treatment (전처리법에 따른 섬유소 기질의 당 함량 변화)

  • Jin, Kilsun;Jeong, Seungmi;Kim, Yongjin;Lee, Donghoon
    • 한국신재생에너지학회:학술대회논문집
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    • 2011.05a
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    • pp.177.2-177.2
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    • 2011
  • 세계적인 자원고갈과 지구온난화와 같은 환경문제가 발생됨에 따라 대체에너지 개발에 대한 연구가 활발히 이루어지고 있다. 섬유소 기질을 이용한 바이오에탄올 생산은 세계적으로 막대한 자원이 있으며 광합성에 의해 재생산되는 무한한 재원으로서 환경적으로도 대기오염물질을 적게 배출하여 유용한 에너지원으로 각광받고 있다. 하지만 섬유소 기질은 cellulose, hemicellulose, lignin과 같은 고분자 화합물이 유기적으로 결합된 단단한 결정구조로 이루어져 있어 이를 분해하여 원하는 물질을 얻기 위해서는 전처리 과정이 필요하다. 전처리 공정은 바이오에탄올을 생산하는 당화 및 발효공정의 효율 및 반응시간 단축에 기여하며, 특히 섬유소 기질일 경우에는 필수불가결한 공정이다. 전처리 공정은 물리적, 화학적, 생물학적 방법으로 나누어지며, 이러한 방법들 중 기질의 특성과 처리효율에 따라 기술들을 병합하여 사용하기도 한다. 이에 본 연구에서는 산 처리, 암모니아 처리, 과산화수소 처리 및 효소를 이용한 생물학적 처리를 단독 또는 병행하여, 전환된 당 성분 및 함량을 조사함으로서 섬유소계 기질인 채소 음식물류 쓰레기를 대상으로 바이오에탄올을 경제적으로 생산하기 위한 적합한 전처리법을 검토하였다. 전처리 방법별 당화율을 살펴보면, 산 처리와 암모니아-과산화수소-계면활성제 처리가 각각 65.3 % 및 65.7 %로 가장 높았으며, 과산화수소 처리는 16.2 %로 가장 낮았다. 반면 전처리 공정 없이 효소를 이용한 당화만을 실시한 경우에는 4.3 %의 낮은 당화율을 나타내었다. 섬유소계 기질의 전처리 효율을 향상시키기 위해 첨가하는 계면활성제의 효과는 암모니아-과산화수소 및 암모니아-과산화수소-계면활성제 처리의 당화율을 비교한 결과, 뚜렷한 효과를 확인할 수 없었다. 전처리 방법별 당의 성분 및 함량을 비교한 결과 육탄당은 암모니아-과산화수소-계면활성제 전처리에서 가장 많이 검출되었다. 오탄당은 산 처리 후 그 함량이 현저히 높았으며, 오탄당 중 xylose의 함량이 60.49 mg/g로 가장 많이 차지하고 있었다. 이 결과로부터 전처리 방법에 관계없이 당화율은 유사한 수준을 보이지만, 당화된 당의 성분 및 함량에는 큰 차이가 있음을 알 수 있었다. 이당류의 경우 과산화수소 및 암모니아-과산화수소 처리를 제외한 나머지 전처리 방법에서 유사한 수준을 나타내었다. 암모니아 처리 및 과산화수소 처리를 순차적으로 병행한 암모니아-과산화수소 처리에서는 각각의 처리시보다 육탄당의 함량은 증가하였으나 암모니아 처리시보다 이당류의 함량은 감소한 것으로 나타났다.

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Postnatal Ontogeny of Expression of Monocarboxylate Transporters(MCTs) and Two Regulatory Proteins, Basigin and Embigin, in The Epididymis of Male Rat (흰쥐의 부정소에서 Monocarboxylate Transporters(MCTs)와 조절 단백질, Basigin과 Embigin의 생후 발달 과정 동안 발현 양상)

  • Lee, K.H.
    • Journal of Animal Science and Technology
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    • v.50 no.1
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    • pp.45-56
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    • 2008
  • In the present study, real-time PCR was performed to evaluated expression of several isoforms of monocarboxylate transporters(MCTs) and two known MCT regulatory proteins, basigin (Bsg) and embigin, in the epididymis of the male reproductive tract during postnatal development. In addition, ERα�-mediated regulation of MCT1 expression in the epididymis was determined with estrogen receptor(ER) α� knockout(α�ERKO) mice by immunohistochemistry. Results from the current study demonstrated differential expression of MCT isoform(MCT 1, 2, 3, 4, and 8), Bsg, and embigin mRNAs in rat epididymis according to postnatal age and epididymal region. In addition, immunohistochemical study of MCT1 revealed the limited localization of MCT1 at apical area of corpus and caudal epididymis. The present study also showed that expression of MCT1 was not directly regulated by ERα�. The findings from the current study suggest that MCTs would involve in establishing adequate microenvironment for sperm maturation and storage in the epididymis, eventually leading to maintenance of male fertility.

Development of Mulberry-leaf Tea Containing γ-Aminobutyric Acid (GABA) by Anaerobic Treatments (무산소 처리에 의한 감마아미노뷰티르산(γ-Aminobutyric Acid) 함량이 높은 뽕잎차의 제조)

  • Lee, Seon-Ho
    • Korean Journal of Food Science and Technology
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    • v.47 no.5
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    • pp.652-657
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    • 2015
  • To produce mulberry-leaf tea abundant in ${\gamma}$-aminobutyric acid (GABA), mulberry leaves were subjected to two distinct anaerobic conditions ($N_2$ and vacuum) for 12 h before the manufacturing process. Subsequently, changes in the GABA content as well as that of other components were measured. In anaerobically treated mulberry leaves, GABA content markedly increased by 436-472% compared with the control, while the glutamic acid content decreased. However, few changes were observed in the contents of the general components (moisture, carbohydrate, lipid, protein, and ash) and water-soluble solids. Free sugar, catechin, and total phenol content decreased after anaerobic treatment. However, the sensory test scores were not different between the control and anaerobically-treated samples. Consequently, tea products, manufactured post nitrogen gas or vacuum treatment of leaves after harvest, showed functional properties without sensory loss.

Process variables of gamma-type aluminum trihydride in wet chemical synthesis (감마형 삼수소 알루미늄 습식합성반응의 공정변수 연구)

  • Yang, Yo-Han;Kim, Woo-Ram;Gwon, Yoon-Ja;Park, Mi-Jeong;Kim, Jun-Hyung;Cho, Young-Min
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.1
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    • pp.214-222
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    • 2018
  • Alane(aluminum trihydride, $AlH_3$) is a candidate material involving high energetic capacity for solid propellant or explosives. In this study aluminum trihydride-etherate ($AlH_3{\cdot}(C_2H_5)_2O$) was synthesized through a wet process, and solid alane was extracted by controlled crystallization. Alane crystals were grown during the crystallization step with phase conversion of aluminum trihydride-etherate to alane using an anti-solvent. Stable crystal forms were found by a 2 hour crystallization process at $85^{\circ}C$. Finally the extracted solid aluminium trihydride consisted mainly of ${\gamma}-type$ with $50-100{\mu}m$ in size.

Isolation and characteristics of yellow-pigment producing mutants of Monascus anka. (Monascus anka로부터 황색소 생성 변이주의 분리 및 특성)

  • 이호재;이형주
    • Microbiology and Biotechnology Letters
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    • v.30 no.2
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    • pp.111-115
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    • 2002
  • To produce yellow pigment selectively, mutants were induced from Monascus anka Nakazawa et Sato IFO 4478 (KCCM 11832 strain), and their characteristics were evaluated. Five kinds of auxotrophic mutants which required amino acids for growth and pigmentation, were isolated through a series of mutagenic treatments. Especially, asparagine auxotroph Y7 produced high ratio of yellow pigment. This mutant showed all the morphological characteristics of Monascuceae but the shape of colony and the diameter of conidia. Mutant Y7 was propagated by sexual reproduction more often than asexual reproduction, which could be effective in production of pigments. Yellow pigment produced extracellularly by the mutant Y7 was more soluble in polar solvents such as ethanol and water than in nonpolar solvents. Its productivity of yellow pigment was 2.2 times higher in the mutant Y7 than in parents. In addition, its yellow pigment showed characteristics of maximum absorption at 373 nm. Moreover, the hue of pigment produced by the mutant Y7 was bright yellow, and it was stable through the subculture over 10 generations.

Compuationally Efficient Propagator Method for DoA with Coprime Array (서로소 배열에서 프로퍼게이터 방법 기반의 효율적인 도래각 추정 기법)

  • Byun, Bu-Guen;Yoo, Do-Sik
    • Journal of Advanced Navigation Technology
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    • v.20 no.3
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    • pp.258-264
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    • 2016
  • In this paper, we propose a computationally efficient direction of arrival (DoA) estimation algorithm based on propagator method with non-uniform array. While the co-prime array techniques can improve the resolution of DoA, they generally lead to high computational complexity as the length of the coarray aperture. To reduce the complexity we use the propagator method that does not require singular value decomposition (SVD). Through simulations, we compare MUSIC with uniform lineary array, propagator method with uniform linear array, MUSIC with co-prime array, and the proposed scheme and observe that the performance of the proposed scheme is significantly better than MUSIC or propagator method with uniform linear array while it is slightly worse than computationally much more expensive co-prime array MUSIC scheme.

EFFECTS OF COPPER AND SALT ON THE HEMOGLOBIN OF SEVERAL FRESHWATER FISHES (담수어류 수종의 혈색소에 미치는 동과 염의 영향)

  • PARK Young Shik;LEE Choon Koo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.5 no.4
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    • pp.105-107
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    • 1972
  • The effects of copper and salt on the hemoglobin patterns of Carassius carassius, Ophicephalus argus, and Misgurnus anguillicaudatus were investigated by vertical starch gel electrophoresis. The number of the hemoglobin band of normal C. cararsius on the anode was one, and that of O. argus was two; neither the number of the homoglobin bands of the fishes nor the mobility of them was affected by copper or salt. The hemoglobin mobility of O. argus was faster than those of C. carassius and M. anguillicaudatus. Normal M. anguillicaudatus had two hemoglobin bands with nearly identical mobilities, one band migrating anodically and the other cathodically. When M. anguillicaudatus was exposed to copper, the mobility of band on the anode was faster than those of the other groups of control and exposed to salt, whereas the hemoglobin band on the cathode was not affected.

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Concentration Distribution of Volatile Organic Compounds in the Ambient Air of an Industrial Shipbuilding Complex : A Case Study (조선소 밀집지역의 휘발성유기화합물 농도분포 특성에 관한 사례 연구)

  • Lee, Myoung-Eun;Park, Eun-Ok;Chung, Jae-Woo
    • Journal of Korean Society of Environmental Engineers
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    • v.37 no.6
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    • pp.380-386
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    • 2015
  • In this study, the spatial and temporal distribution of volatile organic compounds (VOCs) in an industrial shipbuilding complex was monitored, and the local characteristics estimated. The major VOC component measured at all of the sampling sites were almost the same as those found at an indoor painting facility, indicating that painting works conducted in the shipyards were a significant source of VOCs in the vicinity of shipbuilding areas. The main parameters affecting the VOCs distribution were the distance between the sampling site and painting work place, and the intensity of the painting works. A quite broad range of VOC concentrations were observed in all of the sampling sites, depending on the intensity of painting works in nearby areas suppressing the typical seasonal and temporal variation trends in VOCs observed in urban areas.

Effect of Pre-chlorine and Polyamine Dosing for Microcystis sp. Bloomed Water on Drinking Water Treatment Processes : Particle Matter Distribution (Microcystis sp.로 수화된 상수원수에 전염소 및 폴리아민 투입이 정수처리에 미치는 영향 : 입자상 물질 분포)

  • Son, Hee-Jong;Kim, Sang-Goo;Lee, Jeong-Kyu;Hwang, Young-Do;Ryu, Dong-Choon
    • Journal of Korean Society of Environmental Engineers
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    • v.39 no.10
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    • pp.556-560
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    • 2017
  • This research carried out to evaluate the disadvantage of pre-chlorination and the effect of polyamine as coagulant aids for treating the blooming water with Microcystis sp.. Pre-chlorination on blooming water makes the colony of Microcystis sp. to the smaller size. Coagulation with polyamine advanced treatment efficiency not only turbidity but also particulate matters especially less then $5{\mu}m$ size for the blooming water compared with using alum alone. Particle count was more sensitive than turbidity on water quality management of settlement and filtrate.

Design of Luma and Chroma Sub-pixel Interpolator for H.264 Motion Estimation (H.264 움직임 예측을 위한 Luma와 Chroma 부화소 보간기 설계)

  • Lee, Seon-Young;Cho, Kyeong-Soon
    • The KIPS Transactions:PartA
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    • v.18A no.6
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    • pp.249-254
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    • 2011
  • This paper describes an efficient design of the interpolation circuit to generate the luma and chroma sub-pixels for H.264 motion estimation. The circuit based on the proposed architecture does not require any input data buffering and processes the horizontal, vertical and diagonal sub-pixel interpolations in parallel. The performance of the circuit is further improved by simultaneously processing the 1/2-pixel and 1/4-pixel interpolations for luma components and the 1/8-pixel interpolations for chroma components. In order to reduce the circuit size, we store the intermediate data required to process all the interpolations in parallel in the internal SRAM's instead of registers. We described the proposed circuit at register transfer level and verified its operation on FPGA board. We also synthesized the gate-level circuit using 130nm CMOS standard cell library. It consists of 20,674 gates and has the maximum operating frequency of 244MHz. The total number of SPSRAM bits used in our circuit is 3,232. The size of our circuit (including logic gates and SRAM's) is smaller than others and the performance is still comparable to them.