• Korean Journal of Organic Agriculture
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• v.22 no.3
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• pp.503-519
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• 2014

This study was conducted to investigate ginger growth and its nutrient uptake depending on changes of soil properties as affected by applications of green manure crop and/or charcoal in continuous cropping system. The green manure crops applied were barley and hairy vetch, and charcoal was additionally treated in selected plots as a soil conditioner. Experimental plots were prepared as Plot 1 (control), Plot 2 (barley of 8kg $10a^{-1}$), Plot 3 (hairy vetch of 12kg $10a^{-1}$), Plot 4 (charcoal of 1,000kg $10a^{-1}$ and barley 8kg $10a^{-1}$), and Plot 5 (charcoal of 1,000kg $10a^{-1}$ and hairy vetch of 12kg $10a^{-1}$) with two different soil conditions (high clay content, HCC and low clay content, LCC). When comparing selected chemical properties of soils before and after cultivating ginger plant, soil pH decreased from 6.9~8.1 to 6.8~7.6, and electrical conductivity (EC) also declined from $0.45{\sim}1.25dSm^{-1}$ to $0.30{\sim}0.61dSm^{-1}$. However, the content of soil organic matter (SOM) and total nitrogen (T-N) increased. Thus, the soil chemical properties were improved with the applications of green manures and charcoal. Also, macro- and micro-nutrient contents of ginger plants in the different plots were various between normal and diseased plants grown in soils with HCC and LCC. In particular, the concentration of manganese (Mn) was 3~4 folds higher in the diseased plant than in the normal plants. Ginger growth status and yield was relatively improved with the applications of green manures and charcoal as comparing with control plot. Especially in the Plot 4 with LCC, the ginger plant was not infected by root-rot disease.

• 한국해양학회지
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• v.27 no.3
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• pp.250-258
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• 1992

The oxygen isotope composition of surface waters in the Bransfield Strait was determined as one extra state variable in order to characterize water masses in the region, since salinity is significantly modified due to the freezing and ice-melting in the polar region. The salinity, temperature, and $\delta$/SUP 18/O values vary from 34.0 to 34.5$\textperthousand$, -.05 to 2.1$^{\circ}C$ and -0.50 t -0.26$\textperthousand$, respectively. The combined effects of evaporation, precipitation, freezing, ice-melting are reflected in the widely scattered data. Although it is small, the distribution of $\delta$/SUP 18/O of the Bransfield Strait is strongly affected by the freezing-ice melting rather than the evaporation-precipitation. The ice melted fresh water which has higher temperature, depleted salinity and nutrients may be injected to the Bransfield Strait from the north. The concentrations of nutrients are decreasing gradually from the north to the south. The waters were characterized by two groups of higher (about 19.4) and lower N/P ratio (about 16.7). The lower N/P ratio is found in the northern part where ice-melted fresh water is injected. and the higher N/P ratio is found in the southern part of the Bransfield Strait. Although more precise work is needed, the deference of N/P ratio can be an evidence of the ice melted water injection to the Bransfield Strait. Chlorophyll a concentrations, in general, increase from northwest (Waddell Sea) to the southeast (Smith and Hosseason Islands). Probably the injection of nutrient depleted fresh water from the ice melting reduce the chlorophyll a concentration.

• Korean Journal of Ecology and Environment
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• v.35 no.3 s.99
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• pp.160-171
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• 2002

The impact of summer monsoon on water quality of the lower Nakdong River was evaluated during the summer (June-August) in 1997. Several limnological variables were measured in the interval of $1{\sim}3$ day using an automatic monitoring system (Hydrolab $Recorder^{TM}$) to detect water quality changes caused by rainfall on onehour basis. During the monsoon period (from late June to mid July), 5 times of major rainfall events of >50 mm were recorded in the river basin. Dynamic changes of water quality were observed during the monsoon, and the first rainfall event (June$25{\sim}27$) had a significant influence on the water quality at the lower part of the river. All Parameters were largely changed due to the first rain event, and the changed level was maintained until the end of monsoon period. Nutrient concentrations and turbidity increased and values of the other parameters were declined as a result of water dilution. This rainfall event, Changma, is a meteorological phenomenon caused by the East-Asian monsoon climate. The magnitude and frequency of the rainfall during the early monsoon play an important role in change of water quality and ecosystem characteristics of large river systems.

• Journal of The Korean Society of Grassland and Forage Science
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• v.34 no.1
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• pp.39-44
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• 2014

This study was conducted to investigate the effect of the addition of lactic acid bacteria (LAB) and chlorella on the change of nutritive value, quality and microbes regarding the mixture silage of Italian ryegrass and hairy vetch (IRG-HV silage). The content of crude protein of IRG-HV silage in only the LAB treatment was not different as compared to the control. However, the content of crude protein significantly increased in the treatment of LAB with chlorella, compared to both the control and LAB treatments (p<0.05). Acid detergent fiber, neutral detergent fiber, total digestible nutrient and in vitro dry matter digestibility of IRG-Alfalfa silage were not significantly different in all treatments. Further, the pH of IRG-HV silage in only the LAB treatment was not different as compared to control, but decreased in both LAB and chlorella treatments (p<0.05). The content of lactic acid in the LAB treatment increased as compared to the control, and also significantly increased in the LAB treatment with chlorella (p<0.05). In addition, the number of LAB in LAB treatment increased as compared to the control, and significantly also increased in the LAB treatment with chlorella (p<0.05). Therefore, we suggest that IRG-HV silage improved with addition of both LAB and chlorella.

• Korean Journal of Food Science and Technology
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• v.45 no.1
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• pp.25-33
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• 2013

Clenbuterol and ractopamine, which are ${\beta}$-agonists, have been misused as a growth promoting agent in meat producing animals. Clenbuterol was banned for veterinary drug in Korea because of its problems regarding safety. Due to their adverse effects, such as cardiovascular and central nervous diseases on human health proper control and monitoring should be conducted. The existing analytical method of clenbuterol and ractopamine in the Food code was improved through our present study. The bovine muscle samples were subjected to enzymatic hydrolysis, extracted with ethyl acetate and defatted by hexane-methanol partitioning. A molecular imprinted polymer (MIP) solid phase extraction cartridge was used for clean-up and LC-MS/MS was operated in positive multiple reaction monitoring (MRM). Clenbuterol-$d_9$ and ractopamine-$d_3$ were used as an internal standard. The renewed method was validated according to the CODEX guideline. The limits of quantitation for clenbuterol and ractopamine were 0.2 and 0.5 ${\mu}g/kg$, respectively. The mean recoveries ranged in 104.2-113.5% for clenbuterol and in 107.6-118.1% for ractopamine. The improved method was able to save both time and expenses.

• Journal of Food Hygiene and Safety
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• v.29 no.4
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• pp.340-346
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• 2014

In this study, the detection method was developed using real-time PCR to distinguish 4 species (bovine, porcine, horse, and chicken) of raw meats. The genes for distinction of species about meats targeted at 12S rRNA and 16S rRNA parts in mitochondrial DNA. Probes were designed to have a 5' FAM and a TAMRA at the 3' end. This study is to develop 4 species-specific primer and probes about raw materials and real-time PCR on 10 strains to observe the products of non-specific signal for similar species. As a result, any non-specific signal were not detected among each other. Real-time PCR method was developed for quantitation and identification of intentional and unintentional mixture in ground mixed meat (The difference of $C_T$ value between intentional mixture and 100% meat: $${\leq_-}$$ cycles, The difference of $C_T$ value between unintentional mixture and 100% meat: $${\geq_-}$$ cycles). The detection and differentiation of intentional and unintentional mixture in this study would be applied to food safety management for eradication of adulterated food distribution and protection of consumer's right.

• Journal of Food Hygiene and Safety
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• v.32 no.2
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• pp.147-153
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• 2017

The purpose of this study was to isolate Escherichia coli from flies and to assess pathogenic genes and antibiotic resistance of the isolates. A total of 188 flies were captured in agricultural environment including fruits farms (n = 19), fermented soybean farms (n = 9), municipal waste (n = 46), livestock farms (n = 66), slaughterhouses (n = 38), and manure ground (n = 10). E. coli isolates of captured flies were tested for pathogenic gene and antibiotic resistance using PCR methods and VITEK2 systems. As a result, E. coli from 63% (119/188) of the captured flies has been detected, and the detection rate of E. coli was the highest (89%, 31/34) in flies captured at particular slaughterhouse. Of the 34 isolates, 94% (32/34) were pathogenic gene (ST gene) positive. Twenty-six percent (31/119) of the E. coli isolates were observed being resistant to one or more antibiotics. Markedly, one of E. coli isolates from Livestock farms was resistant to 7 antibiotics including ampicillin, ampicillin/sulbactam, cefazolin, cefotaxime, gentamicin, levofloxacin, and trimethoprim/sulfamethoxazole. In addition, it was ESBL positive. The results of the present study may suggest a risk of transmission of pathogenic and antimicrobial resistant bacteria from flies to livestock environment Therefore, it may need to prevent introducing flies into the agricultural production environment for safe food production.

• The Journal of the Korean Society for Microbiology
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• v.8 no.1
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• pp.7-11
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• 1973

The authors identified eighty-eight Shigella cultures among about four thousands specimens collected from all over the country in 1972. Of eighty-eight cultures, seventy-seven cultures belonged to Shigella flexneri and eleven cultures to Shigella sonnei. None of cultures belonging to either subgroup A or C was detected in 1972. Of seventy-seven cultures of Shigella flexneri one was $B_{1b}$, fifty-six were $B_{2a}$, nine were $B_{3a}$, six were $B_{4a}$, three were By and one was each of $B_{3b}$ and $B_{3c}$. Of eleven cultures of Shigella sonnei seven cultures appeared to be phase I and the others phase II. Although there was quite a difference found in the incidence of isolating Shigella organisms between different areas as shown in Table 1, it would not be possible to understand that there might not have been the cases or carriers of Shigella in the areas where the organisms were not isolated in 1972. Concerning the biochemical properties it was not possible to compare the results obtained from the decarboxylase and dihydrolase tests with them obtained in previous years except that of lysine decarboxylase tests since they were not reported individually by the different serotypes in the previous reports. These results obtained in 1972 would be the data for the future comparison. In regards to the antibiotics-sensitivity of Shigella cultures the most of them showed sensitive results to nitrofurantoin, ampicillin, cephalosporin, gentamycin and geopen, and the majority of them appeared to be resistant to cloxacillin, tetracycline and streptomycin by means of the In Vitro tests.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.19 no.1
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• pp.1-11
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• 1983

Data on the trawl operation was compiled from the Korea stern trawlers operated in the sea off the Sahara coast of Africa from May 1975 to April 1976. The distribution of some important demersal fishes were investigated by calculating the catch per haul in every fishing ground sections divided by every fishing ground sections divided by every 30' of latitude and longitude. The results obtained are as follows; 1. The mean catch per haul calculated as: squid 14.9kg, large cuttlefish 29.9kg, small cuttlefish 37.8kg, octopus 44.1kg, sole 8.0kg, seabream 9.3kg and miscellaneous fishes 63.1kg. Where cuttlefish is divided into two sizes, large or small by if it weighs over 300kg or not. 2. Squid were caught mostly from August to November in the northern part of 24$^{\circ}$30'N and southern part of 23$^{\circ}$30'N. 3. Large cuttlefish were caught mostly from December to May of the next year, in the coast from 23$^{\circ}$00'N to 25$^{\circ}$00'N, where as small cuttlefish were caught mostly from April to June and from November to January of next year, in the coast from 23$^{\circ}$30'N to 25$^{\circ}$00'N. 4. Octopus were caught mostly from September to January of the next year and from March to April. in the coast from 23$^{\circ}$00'N to 25$^{\circ}$00'N rather in the offshore than in the near coast. 5. Miscellaneous fishes including seabream were caught from May to November, sole from June to November and the others from May to October.

• Food Science of Animal Resources
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• v.27 no.4
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• pp.531-537
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• 2007

Small amounts of trans fatty acids exist naturally in beef and dairy foods. Also, they can be produced in the process of partial hydrogenation to manufacture shortning or margarine. They can provide a better palatability and shelf life. According to the recently studies, trans fatty acids can raise health risk such as heart diseases and coronary artery diseases. They can also increase low-density lipoprotein (LDL) cholesterol and decrease high-density lipoprotein (HDL) cholesterol in the blood plasma, therefore increasing the risk of atherosclerosis and diabetes. The aim of this study was to determine total lipids and trans fatty acids (TFAs) content in processed foods and meat products. The analysis of trans fatty acids was performed in 28 samples of donuts, 18 samples of bakeries, 4 samples of frozen doughs, 2 samples of popcorns, and 4 samples of meat products (ham, sausage, nuget, and bacon). Total lipids in processed foods and meat products were extracted by chloroform-Methanol method and acid digestion, respectively. They were analyzed by gas chromatography using a SP-2560 column and flame ionization detector. The amounts of TFAs per 100 g of foods were 0-3.3% (0.74% on average) in donuts, 0.2-5.8% (1.18% on average) in bakeries, 0.2-6.3% (1.93% on average) in frozen doughs, and 0-5.8% in popcorns. Meat products such as ham, sausage, and nuget analyzed 0.1% of TFAs, respectively and trans fatty acids in bacon were not detected. As a result, the distribution of TFAs in processed foods was widely ranged from O% to 6.3% according to manufacturers and types of products, whereas the content of TFAs in meat products ranged from 0% to 0.1%.