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http://dx.doi.org/10.13103/JFHS.2014.29.4.340

Detection and Differentiation of Intentional and Unintentional Mixture in Raw Meats Using Real-time PCR  

Kim, Kyu-Heon (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
Kim, Mi-Ra (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
Park, Young-Eun (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
Kim, Yong-Sang (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
Lee, Ho-Yeon (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
Park, Yong-Chjun (Novel Food Division, Ministry of Food and Drug Safety)
Kim, Sang Yub (Hazardous Substances Analysis, Busan Regional Korea Food and Drug Administration)
Choi, Jang Duck (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
Jang, Young-Mi (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
Publication Information
Journal of Food Hygiene and Safety / v.29, no.4, 2014 , pp. 340-346 More about this Journal
Abstract
In this study, the detection method was developed using real-time PCR to distinguish 4 species (bovine, porcine, horse, and chicken) of raw meats. The genes for distinction of species about meats targeted at 12S rRNA and 16S rRNA parts in mitochondrial DNA. Probes were designed to have a 5' FAM and a TAMRA at the 3' end. This study is to develop 4 species-specific primer and probes about raw materials and real-time PCR on 10 strains to observe the products of non-specific signal for similar species. As a result, any non-specific signal were not detected among each other. Real-time PCR method was developed for quantitation and identification of intentional and unintentional mixture in ground mixed meat (The difference of $C_T$ value between intentional mixture and 100% meat: $${\leq_-}$$ cycles, The difference of $C_T$ value between unintentional mixture and 100% meat: $${\geq_-}$$ cycles). The detection and differentiation of intentional and unintentional mixture in this study would be applied to food safety management for eradication of adulterated food distribution and protection of consumer's right.
Keywords
Real-time PCR (Polymerase Chain Reaction); Raw Meats; mt (mitochondrial) DNA;
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Times Cited By KSCI : 7  (Citation Analysis)
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