• 한국신재생에너지학회:학술대회논문집
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• 2010.11a
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• pp.105.2-105.2
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• 2010

전처리 후 얻어진 셀룰로스 고분자를 단당류로 전환하기 위해서는 셀룰라제를 이용한 당화 과정이 필요하다. 통상 실험식 연구에서는 셀룰로스 당화시 당수율을 최대로 하기위해 pH조절을 위한 Citrate buffer와 미생물 오염을 막기 위한 Autoclave에서의 멸균 과정을 거친다. 하지만 대량생산을 목적으로 하는 산업체에서는 적용이 어렵다는 문제점이 있다. 따라서 본 연구에서 이를 대신하여 산업체에서 적용 가능한 당화전환 공정의 효율성을 평가하고자 하였다. Autoclave 멸균을 대체하는 공정으로 항생제 첨가와 여과에 의한 제균을 선택하였고, citrate buffer를 대신하여 buffer를 첨가하지 않은 물을 pH를 조정하여 사용 하였다.실험결과 기존의 당화공정을 사용하였을 때 당화율이 81%이었고, pH를 조절한 제균 water에 항생제를 첨가하는 공정은 71%로 나머지 배지들 중 가장 높은 당화율을 나타냈다. 이것은 기존의 당화율보다 10% 낮은 수치이나 공정비를 교려하여 봤을 때 효율성 있는 공정으로 판단된다.

• KSBB Journal
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• v.23 no.1
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• pp.1-7
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• 2008

The world demand of ethanol as an alternative fuel for gasoline is increasing rapidly because of high oil price and global climate change. Most of ethanol is currently produced from corn grain or sugars in sugarcane and sugar beet. Because these sources compete with foods and animal feed and are not expected to be enough for future demand of ethanol. Thus, cellulosic ethanol from agricultural residues or wood has to be commercialized in near future. Typical cellulosic ethanol production consists of pretreatment, enzyme hydrolysis, fermentation and product separation. This paper reviews the principles and status of each step and discusses issues for cellulosic ethanol production.

• 한국신재생에너지학회:학술대회논문집
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• 2011.05a
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• pp.179.1-179.1
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• 2011

Pretreatment of cellulosic biomass is necessary before enzymatic saccharification and fermentation. The objective of this study was to evaluate the effect of combined aqueous ammonia-dilute sulfuric acid treatment on cellulosic biomass. Miscanthus was pretreated using aqueous ammonia and dilute sulfuric acid solution under high temperature and pressure conditions to be converted into bioethanol. Aqueous ammonia treatment was performed with 15 %(w/w) ammonia solution at $150^{\circ}C$ of reaction temperature and 20 minutes of reaction time. And then, dilute sulfuric acid treatment was performed with 1.0 %(w/w) sulfuric acid solution at $150^{\circ}C$ of reaction temperature and 10 minutes of reaction time. The compositional variations of this combined aqueous ammonia-dilute sulfuric acid treatment resulted in 68.0 % of cellulose recovery and 95.7 % of hemicellulose, 81.3 % of lignin, 89.1 % of ash removal respectively. The enzymatic digestibility of 90.5 % was recorded in the combined pretreated Miscanthus sample and it was 14.7 times higher than the untreated sample. The ethanol yield in the Simultaneous Saccharification and Fermentation was 90.4 % of maximum theoretical yield based on cellulose content of the combined pretreated sample and it was about 98 % compared to the ${\alpha}$-cellulose ethanol yield.

• New & Renewable Energy
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• v.6 no.4
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• pp.6-14
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• 2010

Pretreatment of cellulosic biomass is necessary before enzymatic saccharification and fermentation. Extrusion is a well established process in food industries and it can be used as a physicochemical treatment method for cellulosic biomass. Aqueous ammonia soaking treatment at mild temperatures ranging from 60 to $80^{\circ}C$ for longer reaction times has been used to preserve most of the cellulose and hemicellulose in the biomass. The objective of this study was to evaluate the effect of extrusion treatment on aqueous ammonia soaking method. Extrusion was performed with miscanthus sample conditioned to 2mm of particle size and 20% of moisture content at $200^{\circ}C$ of barrel temperature and 175rpm of screw speed. And then aqueous ammonia soaking was performed with 15%(w/w) ammonia solution at $60^{\circ}C$ for 1, 2, 4, 8, 12 hours on the extruded and raw miscanthus samples respectively. In the combined extrusion-soaking treatment, most compositions removal occurred within 1~2 hours and on a basis of 1 hour soaking treatment values, cellulose was recovered about 85% and other compositions, including hemicellulose, are removed about 50% from extruded miscanthus sample. The combined extrusion-soaking treated and soaking only treated samples were subjected to enzymatic hydrolysis using cellulase and ${\beta}$-glucosidase. The enzymatic digestibility value of combined extrusion-2 hours soaking treated sample was comparable to 12 hours soaking only treated sample. It means that extrusion treatment can shorten the conventional long reaction time of aqueous ammonia soaking. The findings suggest that the combination of extrusion and soaking is a promising pretreatment method to solve both problems for no lignin removal of extrusion and long reaction time of aqueous ammonia soaking.

• KSBB Journal
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• v.25 no.6
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• pp.533-538
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• 2010

We investigated the effect of inhibitory compounds derived lignocellulosic hydrolysates on cell growth, sugar consumption and ethanol productivity, and also we intended to identify the potential for ethanol production based on lignocellulosic hydrolysates. Cell growth and ethanol production in the presence of acetate were initiated after 12 hr. Furans showed a longer lag time and phenolics showed a significant effect on strain and ethanol production in comparison to other model compounds. In the case of lignocellulosic hydrolysates, the acetate strongly affected cell growth and ethanol production.

• 한국신재생에너지학회:학술대회논문집
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• 2011.11a
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• pp.112.1-112.1
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• 2011

쌀을 주식으로 하는 우리나라의 여건상 연간 추정치로 싸라기 약 12만톤, 미강 약 49만톤, 왕겨 약 79만톤의 벼 도정 부산물이 발생하고 있다. 본 연구에서는 벼 도정 부산물 중 비식량 자원인 왕겨를 대상으로 고효율 효소 당화를 위한 바이오매스 전처리 방법을 탐색하였다. 왕겨 원시료의 초기 조성은 셀룰로스 34.5%, 헤미셀룰로스 20.5%, 리그닌 25.3%, 회분 14.6%로 나타났는데, 억새 등 초본계 바이오매스와 비교하여 특이하게 높은 성분은 회분으로 이는 벼에 대한 규산질 비료의 시용에서 기인한 것이다. 바이오매스 전처리에 많이 사용되는 암모니아, 희황산 용매와 규산염에 침식성을 가지는 가성소다 용매를 이용하여 각 용매별 단독 및 알칼리-산 복합 처리 하였을 때 효소 가수분해 효율, 고상시료 성분변화 등을 상호 비교하였다. 예비실험을 통하여 암모니아 처리조건은 15%(w/w) $150^{\circ}C$ 20분, 가성소다 처리조건은 1.5%(w/w) $150^{\circ}C$ 20분, 희황산 처리조건은 1.0%(w/w) $150^{\circ}C$ 10분으로 설정하였다. 암모니아 단독, 희황산 단독, 암모니아-희황산 복합 처리 시료의 효소 가수분해 효율은 각각 37.8%, 39.1%, 42.8%로 약 40%선에서 큰 차이가 없었다. 반면 가성소다 단독, 가성소다-희황산 복합 처리시료의 효소 가수분해 효율은 각각 62.7%, 82.8%로 나타나 앞선 3가지 처리방법 대비 50%, 100%에 가까운 효소 가수분해 효율 향상을 보였다. 이 때 전처리 고상시료의 성분 변화를 살펴보면 회분 함량에서 큰 차이를 보였는데 암모니아 단독, 가성소다 단독, 희황산 단독, 암모니아-희황산 복합, 가성소다-희황산 복합 처리에서 각각 47.8%, 77.1%, 43.5%, 55.8%, 94.7%의 회분 성분 기각률(rejection rate)을 나타냈다. 이는 왕겨 효소 가수분해 효율의 최대 저해요인이 회분임을 추정할 수 있다. 왕겨 전처리 알칼리 용매는 암모니아보다 가성소다가 더 효과적이었고 희황산 복합 처리시 그 효과가 크게 상승하였다. 따라서 규산염(회분) 함량이 높은 바이오매스는 고온 고압 조건에서 가성소다 용액으로 처리한 후 그 고상분을 희황산 용액으로 복합 처리하는 시스템이 효소 당화 증진에 매우 유리함을 확인하였다.