• Journal of the Korea Institute of Information and Communication Engineering
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• v.20 no.12
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• pp.2395-2400
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• 2016

Conventional drug carriers such as liposomes, nanoparticles, polymer micelles, polymeric conjugate and lipid microemulsion for cancer chemotherapy shield normal tissues from toxic drugs to treat cancer cells in tumors. However, inaccurate tumor targeting uncontrolled drug release from the carriers and unwanted accumulation in healthy sites can limit treatment efficacy with current conventional drug carriers with insufficient concentrations of drugs in the tumors and unexpected side effects as a result. Sickle red blood cells show natural tumor preferential accumulation without any manipulation due to the adhesive interaction between molecular receptors on the membrane surface and counter-receptor on endothelial cells. In addition, structural changes of microvascular in tumor sites enhances polymerization of sickle red blood cells. In this research, we examined the use of sickle red blood cells as a new drug carrier with novel tumor targeting and controlled release properties to quantify its therapeutic effects.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.17 no.5
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• pp.389-395
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• 2024

This study was conducted to determine the cytotoxicity of the extracts from the human body contact area of the test substance during a test on the Good Laboratory Practice (GLP), which is the medical device safety evaluation standard, using the extracorporeal shock wave therapy (ESWT) cartridge as a sample, using L-929 cells. The test and control substances were extracted with 1xMEM culture medium containing 10% FBS at 37±1℃ for 24±2 hours. The test substance extract (test group), negative control substance extract (negative control group), positive control substance extract (positive control group), and blank test solution extract (solvent control group) were applied to L929 cells and cultured for 48±2 hours in a 37±1℃, 5±1% CO2 incubator. As a result of observing cell reactions under a microscope, the cells to which the blank test solution extract and negative control substance extract were applied were grade 0, the cells to which the positive control substance extract was applied were grade 4, and the cells to which the test substance extract was applied were grade 0. As a result of quantitative evaluation through cell counting, the cell viability rate of the cells to which the negative control substance extract was applied was 106.28% compared to the blank test solution extract, the cells to which the positive control substance extract was applied were 0.00%, and the cells to which the test substance extract was applied were 99.58%. Therefore, when the results of the negative and positive control groups were confirmed, the test process was appropriate, and it was determined that it did not cause cytotoxicity because the qualitative evaluation method was less than grade 2 and the quantitative evaluation method showed a cell viability rate of more than 70%.

• Korean Journal of Plant Resources
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• v.29 no.1
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• pp.26-31
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• 2016

Toxoplasmosis is an important cause of foodborne, inflammatory illnesses, as well as congenital abnormalities. Currently available therapies are ineffective for persistent chronic disease and congenital toxoplasmosis or have severe side effects which may result in life-threatening complications. There is an urgent need for safe and effective therapies to eliminate or treat this cosmopolitan infectious disease. The aim of this study was to investigate the in vitro anti-Toxoplasma activities of Plantago asiatica L., one of the herbal extracts, using tachyzoit of T. gondii RH strain infected HeLa cells. As the results, the selectivity of Plantago asiatica L. extract was 4.5, which was higher than Sulfadiazine selectivity (0.4). Also, we perfomed the cell proliferation inhibition test and the morphological study to evaluate the anti-T. gondii activity of Plantago asiatica L. extract with HeLa cells. As the results, the inhibition rate of the Plantago asiatica L. extract was high inhibition rate. This indicates that the Plantago asiatica L. extract may be used for new anti-T. gondii agent.

• Tuberculosis and Respiratory Diseases
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• v.63 no.2
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• pp.154-164
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• 2007

Background: Irinotecan hydrochloride, a topoisomerase I inhibitor, is effective against small-cell lung cancer. Irinotecan also can act as a potential radiation sensitizer along with cisplatin. To evaluate efficacy and toxicity of irinotecan plus cisplatin (IP) with concurrent thoracic radiotherapy, we conducted a phase II study of IP followed by concurrent IP plus hyperfractionated thoracic radiotherapy in patients with previously untreated limited-stage small-cell lung cancer. Methods: Twenty-four patients with previously untreated small-cell lung cancer were enrolled onto the study since November 2004. Irinotecan $60mg/m^2$ was administered intravenously on days 1 and 8 in combination with cisplatin $60mg/m^2$ on day1 every 21 days. From the first day of third cycle, twice-daily thoracic irradiation (total 45 Gy) was given. Prophylactic cranial irradiation was given to the patients who showed complete remission after concurrent chemoradiotherapy. Restaging was done after second and sixth cycle with chest CT and/or bronchosocpy. Results: Up to November 2004, 19 patients were assessable. The median follow-up time was 12.5 months. A total of 99 cycles (median 5.2 cycles per patient) were administered. The actual dose intensity values were cisplatin $19.6mg/m^2$/week and irinotecan $38.2mg/m^2$/week. Among the 19 patients, the objective response rate was 95% (19 patients), with 9 patients (47%) having a complete response (CR). The major grade 3/4 hematological toxicities were neutropenia (35% of cycles), anemia (7% of cycles), thrombocytopenia (7% of cycles). Febrile neutropenia was 4% of cycles. The predominant grade 3/4 non-hematological toxicities was diarrhea (5% of cycles). Toxicities was not significantly different with concurrent administration of irinotecan and cisplatin with radiotherapy, except grade 3/4 radiation esophagitis (10% of patients). No treatment-related deaths were observed. The 1-year and 2-year survival rate of eligible patients was 89% (16/18) and 47% (9/18), respectively. Conclusion: Three-week schedule of irinotecan plus cisplatin followed by concurrent IP plus hyperfractionated thoracic radiotherapy is an effective treatment for limited disease small-cell lung cancer, with acceptable toxicity.

• The Journal of the Korea Contents Association
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• v.10 no.9
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• pp.309-317
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• 2010

The stainless steel wire is extensively used for the orthodontic treatment. But, the stainless steel wire that has commonly superior corrosion resistance has caused hypersensitive reaction or allergy as side effects because of corrosion in the oral environment. For improving the problem of corrosion, we was evaluated the suitability of the duplex stainless steel(DSS) as orthodontic wire through this study. The DSS wire was evaluated the mechanical strength and bio-stability for suitability and bio-compatibility as orthodontic wire. In this work, the DSS and stainless steel(SS) as common use of medical grade were prepared for the tensile strength test. The DSS wire were treated by heat. and Temperature conditions of the heat treatment were $28^{\circ}C$, $500^{\circ}C$, $600^{\circ}C$, $700^{\circ}C$, $800^{\circ}C$, $900^{\circ}C$, respectively. And the DSS wires that treated by heat on the optimum temperature condition were conducted the bending moment test and calculated the S-Max value and the modulus of elasticity. For evaluating the bio stability, each materials were conducted in vitro test for measuring the cell survival rate. The most interesting results was that the tensile strength test of SS wire($8.17\times10^4\;N/mm^2$) and DSS wire($8.05\times10^4\;N/mm^2$) that treated at $500^{\circ}C$ by heat were similar in mechanical strength. In the bio-stability study, the DSS has no cytotoxicity (p=0.05) Thus, we could make a conclusion that the duplex stainless steel wire has vastly superior corrosion resistance was suitable as orthodontic wire.

• Korean Journal of Plant Resources
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• v.26 no.5
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• pp.652-657
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• 2013

In the present study, anti-oxidative and the RMC proliferation inhibitory propeties of 80% ethanol extracts from 63 kinds of traditional medicines were investigated. Inhibitory effects of RMC proliferation were showed that Dalbergia odorifera T. Chen., Melia azedarach Linn$\acute{e}$ and Hydnocarpus anthelmintica Pierre. Among them Hydnocarpus anthelmintica Pierre had the highest anti-oxidative activity ($ORAC_{PE\;value}=1.6$, DPPH = 81.1), but Dalbergia odorifera T. Chen. and Melia azedarach Linn$\acute{e}$ had no effects. These results suggest that the Hydnocarpus anthelmintica Pierre could prevent or protect from kidney disease as antioxidant and anti-proliferative agent for RMC.

• Journal of Life Science
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• v.26 no.10
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• pp.1214-1217
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• 2016

Rebamipide is a mucosal-protective antiulcer drug, but its mechanism of action in gastric cancer remains elusive. CagA, a major virulence factor of Helicobacter pylori (H. pylori), is associated with the risk of gastric cancer. CagA protein is injected into gastric epithelial cells and deregulates a variety of cellular signaling molecules. CagA from H. pylori induces phospholipase D1 (PLD1) expression through NFκB activation in gastric epithelial cells, followed by invasion and proliferation of gastric epithelial cancer cells. Infection with cagA-positive H. pylori and expression of CagA enhances the binding of NFκB to the PLD1 promoter. Rebamipide abolishes H. pylori cagA-induced PLD1 expression via inhibition of binding of NFκB to the PLD1 promoter and also inhibits PLD activity. Moreover, rebamipide abolishes H. pylori CagA-induced β-catenin and the expression of a target cancer stem cell (CSC) marker gene via upregulation of miRNA-320a and -4496, followed by attenuation of self-renewal capacity of H. pylori CagA-infected gastric CSCs. In addition, rebamipide increases the chemosensitivity of CagA-expressed gastric CSCs and suppresses gastric carcinogenesis. Thus, it is speculated that rebamipide might show a potent efficacy as chemotherapeutic drug against gastric cancer cells. In this review, we summarizes recent results regarding the novel insights for the efficacy of rebamipide in gastric cancer cells.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2003.05a
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• pp.121-122
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• 2003

현대의학의 발달에도 불구하고 암은 여전히 치료하기 힘든 질병 중 하나이며 질병에 의한 사망 중에서 암은 심장 질환 다음으로 높은 사망률을 나타내고 있다. 오래 전부터 암을 극복하기 위해 많은 연구가 진행되어 왔으나 아직까지 암을 완전히 퇴치할 수 없는 실정이며, 현재 임상에서 사용되고 있는 항암제의 대부분은 합성물질로써 부작용이 심하여 문제가 되고 있다. (중략)

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.129-129
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• 1993

현재 암의 치료에 이용되고 있는 항암제들은 therapeutic index가 낮아서 면역 및 조혈기능 장애 등 해결 되어야 할 문제점 들을 안고 있다. 이에 본 여구자는 우수하고 안전한 항암제를 개발하여 암의 치료에 이바지하고자, 화학연구소에서 in vitro 세로독성을 확인한 화합물에 대하여 in vivo 항암력을 실험, 치료효과가 높은 유도체/유사체 개발에 필요한 정보를 제공함에 본 연구의 목표를 두고 있다. 1차년도 연구에서는 화학연구소측이 제공한 KS 0409를 실험 약물로, sarcoma 180 복수암에 대한 in vivo 항암력을 실험하였다. 약 4주령의 SPE(specific pathogen-free) ICR계 마우스 및 BALB/c 마우스를 실험동물로 하여 sarcoma 180 암세포 부유액(세포 농도, 1$\times$$10^{7}$ cells/ml)을 실험동물의 복강내에 0.1ml 씩 이식하고 암이식 24시간 후부터 매일 1회씩 9회 약물 주사를 시행하였다. 대조약물 cisplatin은 2% DMSO-생리식염수를 주사하였다. 생존일수 관찰은 60dlfRK지 하였으며 % T/C를 계산하여 항암력을 평가하였다. 단 60일 생존 동물은 평균수명 계산에서 제외하였다.

• Clinical and Experimental Pediatrics
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• v.49 no.4
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• pp.439-445
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• 2006

Purpose : ${\alpha}$-Galactosylceramide (${\alpha}$-GalCer)-stimulated human $V{\alpha}24$ natural killer T (NKT) cells exert antitumor activity against some leukemia in a CD1d dependent and TCR-mediated manner, but could not kill CD1d - negative neuroblastoma (NB) cells. There are few reports about the direct antitumor effect of highly secreted cytokines by these cells on activation. In this study, using a cell-free supernatant (SPN) collected from plate bound hCD1d/${\alpha}$ GalCer tetramers-stimulated NKT cells, we examined whether they could be helpful in the immunotherapeutic treatment of NB. Methods : Cells were cultured in IMDM. The cytokines produced by NKT cells were measured with Cytometric Bead Array (CBA) analysis. Cell viability was evaluated by calcein-AM fluorescence with digital image microscopy scanning (DIMSCAN). The percentage of specific apoptosis was calculated by flow cytometric detection of apoptosis using annexin V and 7-AAD. Results : The activated NKT cells secreted high levels of IL-2, INF-${\gamma}$, TNF-${\alpha}$. The SPN was significantly cytotoxic against four out of eight tested NB cell lines, through mainly apoptosis as evidenced by annexin-V staining and inhibition with the pretreatment of pancaspase blocker. This apoptosis was significantly inhibited when anti-TNF-${\alpha}$ and anti-IFN-${\gamma}$ neutralizing mAbs were used separately and it was completely abolished when the two mAbs were combined. Conclusion : IFN-${\gamma}$ and TNF-${\alpha}$ produced by NKT cells could exert synergistically direct antitumor activity through apoptosis on some NB cell lines.