• Journal of the Society of Cosmetic Scientists of Korea
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• v.39 no.4
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• pp.313-322
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• 2013

Ultraviolet irradiation in the cells and skin produces reactive oxygen species (ROS), which induces the synthesis of matrix metalloproteinases (MMPs) causing skin photoaging. Using the human dermal fibroblast (HDF), we investigated the antioxidative and anti-aging effects of the extracts from Talinum paniculatum. Talinum paniculatum leaf and stem extracts (LSE) showed free radical scavenging effect by 98.45% at 500 ${\mu}g/mL$ and superoxide radical scavenging effect by 97.01% at 500 ${\mu}g/mL$ in the xanthine/xanthine oxidase system. The photoprotective potential of LSE was tested in HDF exposed to ultraviolet irradiation. It was revealed that LSE had an inhibitory effect on MMP-1 expression in UVA-irradiated HDF without any significant cytotoxicity. The treatment of UVA-irradiated HDF with LSE resulted in dose-dependent decreases in the expression levels of MMP-1 mRNA and protein. Also, UVB-induced cytotoxicity and cell death were effectively suppressed by treatment of LSE. Additionally, the senescence-associated ${\beta}$- galactosidase (SA-${\beta}$-gal) activity was decreased in the presence of LSE. These results suggest that Talinum paniculatum leaf and stem extracts (LSE) may have anti-aging effects and can be used as new functional materials against oxidative stress-mediated skin damages.

• Journal of Applied Biological Chemistry
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• v.60 no.1
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• pp.13-17
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• 2017

To develop a new functional agent for cosmetics, we investigated the anti-aging activities in fibroblasts of casuarictin. The anti-aging effect of casuarictin in CCD-986sk cell was as follows: it inhibited ROS expression increased by Ultraviolet B and suppressed pro-collagen expression. Also, casuarictin had inhibited Matrix metalloproteinase-1 expression. Therefore, the results suggested that casuarictin has considerable potential as a cosmetics ingredient with a anti-aging effect.

• Journal of Life Science
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• v.29 no.10
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• pp.1071-1079
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• 2019

In this study, we investigated the antioxidant and anti-aging activities of Schisandra chinensis seed fractions by adding them to UVB-irradiated HaCaT cells and analyzing the suppression of matrix metalloproteinases (MMPs). An electron-donating assay, ABTS radical scavenging assay, and hydrogen peroxide scavenging assay showed that the ethyl acetate fraction of S. chinensis seed (SCEAf) has scavenging activities. A collagenase inhibition activity assay showed that SCEAf had inhibitory effects of over 92.3% at $500{\mu}g/ml$ concentration. An MTT assay was performed to determine the cytotoxicity of SCEAf in HaCaT cells and the results showed that SCEAf increased cellular viability in a concentration-dependent manner. In addition, SCEAf was found to increase the viability of cells irradiatged by UVB $50mJ/cm^2$. To examine the anti-aging effects of SCEAf on HaCaT cells irradiated with UVB $50mJ/cm^2$, the expression of MMP-1 and -3 was analyzed by Western blotting and reverse-transcription polymerase chain reaction. The results showed that MMP-1 and -3 proteins and mRNA levels were downregulated in a concentration-dependent manner in response to SCEAf. These results suggest that SCEAf can prevent aging and alleviate aging symptoms by inhibiting collagenase activity in skin keratinocytes damaged by UVB. Therefore, S. chinensis seeds may have the potential for use as functional ingredients with anti-aging effects in the cosmetic and food industries.

• Journal of Life Science
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• v.32 no.8
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• pp.647-658
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• 2022

Particulate matter (PM) is known to be involved in the onset and progression of various diseases by promoting oxidative and inflammatory reactions as air pollutants containing various small particles that are harmful. In this study, the protective efficacy of herbal medicines was evaluated in human corneal epithelial cells (hCECs) to select natural products that can protect the eye, the primary organ directly exposed to external pollutants from PM. As a result, five candid ate herbal medicines [Cheonmundong, Asparagus Rhizome; Seokchangpo, Aciru Gramineri Rhizoma; Hwangryeon, Coptidis Rhizoma; Gamgug, Chrysanthemi Indici Flos; and Geumjanhwa (Marigold flower petals)] which showed inhibitory efficacy on PM_2.5-induced cytotoxicity, were selected from among 12 candidate herbal medicines. To evaluate the antioxidant activity of these candidate substances, the reactive oxygen species (ROS) scavenging ability was investigated, and it was found that the extracts of Seokchangpo, Cheonmundong and Hwangryeon showed a significant inhibitory effect on PM_2.5-induced ROS production, which was correlated with the preservation of mitochondrial activity. In addition, it was confirmed that they could block DNA damage caused by PM_2.5 through analysis of 8-hydroxy-2'-deoxyguanosine generation and phosphorylated-H2A histone family member X (γ- H2AX) expression. Furthermore, the increase in inflammasome activity and inflammatory response in PM_2.5-treated hCECs was also canceled in the presence of these extracts. Although additional studies are needed, the results of this study will be used as primary data to find novel natural compounds that protect hCECs from PM.

• Journal of Life Science
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• v.33 no.2
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• pp.208-215
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• 2023

In recent decades, the field of aging research has progressed from the genetic and cellular levels to in vivo models of blood exchange. Since genes capable of extending the lifespan in C. elegance have been reported, various potential target molecules have been discovered through genomics, proteomics, metabolomics, and transcriptomics. Accordingly, research on the interactions between target molecules has also been increasing. The parabiosis method, in which two experimental animals are surgically combined, was introduced, and a factor that could reverse the aging phenomenon was discovered using this method. The parabiosis method is used to find more accurate and effective aging-reversal factors that could exist in young blood. As more new evidence has been revealed, the parabiosis method has established a new paradigm for aging research. Moreover, a device capable of exchanging blood elaborately in laboratory animals was published in 2022 and presented new results necessary for aging reversal. Since GDF11, was reported, many other anti-aging candidates that are soluble factors in blood, such as β2m, TIMP2, VCAM1, Gpld1, and clusterin, have been discovered. In addition, mcicroglia cells and neuroinflammation have been directly proven to be aging factors. These latest research results were obtained by parabiosis, the newly designed device for plasmapheresis, and injecting young blood or conditioned blood methods. In this review, we discuss the latest research results using the device and young blood administration in old mice.

• Proceedings of the KSME Conference
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• 2008.11a
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• pp.1674-1678
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• 2008

본 연구에서는 심장의 세포 변화에서부터 혈류 순환의 시스템 변화까지 일련의 과정을 시뮬레이션 할 수 있는 통합모델을 개발하였다. 본 통합 모델을 이용하여 대동맥의 탄성도 변화 따른 Pulse Wave Velocity를 추정하였으며 심근의 수축 Mechanics의 변화를 시뮬레이션 하였다. 심장은 단순한 구 형상으로 모델링 되었다. 특히 동맥순환의 특성인 Wave propagation 과 Wave deflection의 현상을 모델링하기 위해 기존 모델에서 사용된 동맥계 순환 모델을 수정하였다. 즉 기존의 동맥 모델을 1차원의 운동방정식과 연속방정식을 기반으로 하는 Distributed arterial model로 대체하였다. Distributed arterial model은 혈액의 점성에 의한 에너지 손실, 혈관의 점탄성 효과 그리고 분지 되는 혈관에서의 에너지 손실을 포함하는 정교한 동맥 순환 모델이다. 정교한 동맥계 순환 모델의 동맥 탄성도 값을 조절함으로써 탄성도 변화에 대한 PWV를 계산 할 수 있었다. 이러한 수치적 방법을 사용하여 노화에 따른 동맥벽 탄성도의 저하가 심근세포의 Cross-bridge 동역학에 미치는 영향을 시뮬레이션 하였다.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.4 s.48
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• pp.445-448
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• 2004

Aging is divided into intrinsic aging and photo-aging. Intrinsic aging is naturally occurred as the time passed and photo-aging is induced by the UV radiation of skin. The main reason of aging is the free radicals and the degeneration of the cellular materials by free radicals. In this paper, we checked the anti-aging effects of Mallotus japonicus bark extracts. It has the ability to scavenge free radicals and the SOD like activity. Also, it reduced the cell damage by hydrogen peroxide treatment. Mallotus japonicus bark extracts showed the excellent activity on inhibiting the UV induced cell damage and DNA damage. In conclusion, Mallotus japonicus bark extracts can be used as active ingredients for anti-aging cosmetics.

• Journal of Life Science
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• v.34 no.2
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• pp.94-104
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• 2024

β-Lapachone is a natural quinone compound originally obtained from the bark of the lapacho tree (Tabebuia vellanedae), which has been used in traditional medicine in several South and Central American countries for treating various diseases. Although β-lapachone has been reported to have potent anticancer activity in many types of cancer cells, its effect on the proliferation of hepatocellular carcinoma (HCC) cells is still unclear. Therefore, in this study, we investigated the effect of β-lapachone on the proliferation of human HCC Hep3B cells. According to our results, the decrease in cell viability of Hep3B cells caused by β-lapachone was closely related to the induction of apoptosis, which was confirmed through changes in nuclear morphology and flow cytometry. In addition, in Hep3B cells treated with β-lapachone, the expression of Bcl-2, an anti-apoptotic factor, was decreased, while the expression of Bax, an apoptosis inducer, was increased, and the activity of the caspase cascade was also increased. However, in the presence of a pan-caspase inhibitor, β-lapachone-induced apoptosis was weakened, indicating that the induction of apoptosis by β-lapachone was caspase-dependent. Moreover, β-lapachone treatment activated extracellular-regulated kinase (ERK) signaling while inhibiting activation of the phosphoinositide 3 kinase (PI3K)/Akt pathway. Furthermore, the effect of the ERK inhibitor on suppressing the induction of apoptosis by β-lapachone was minimal, and the PI3K inhibitor significantly increased β-lapachone-induced apoptosis. The findings from this study will contribute to a better understanding of the anticancer activity of β-lapachone in HCC cells.

• KSBB Journal
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• v.23 no.5
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• pp.431-438
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• 2008

For the experiment, to develop new materials for cosmetics, the Celosia cristata L. plant ethanol extract were used for physiological effect and cosmetics application research. The Celosia cristata L. is a Korean traditional variety grown. To investigate the effect of Ethanol extract of Celosia cristata L. on skin care, we measured anti-oxidant activity and anti-aging activity. Celosia cristata L. ethanol extract itself had anti-oxidant activity in a dose-dependent manner in 1-diphenyl-2-picryl-hydrazyl(DPPH) radical scavenging. Ethanol extract had anti-oxidant activity in a dose-dependent manner. Silica dose-dependently increased the intracellular ROS generation in RAW 264.7 cells. Celosia cristata L. ethanol extract inhibited silica-induced intracellular superoxide anion generation and $H_2O_2$ generation and hydro-peroxide generation in RAW 264.7 cells. For anti-aging effects, the hyaluronidase inhibition effects, were relatively strong and they also showed elastase activity inhibition effects, which suggesting the Celosia cristata L. ethanol extract might be used as hydration and anti-wrinkle agents. From the above results, it is referred that Celosia cristata L. ethanol extract appears to have potent anti-oxidant activity and anti-aging activity.

• Journal of Life Science
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• v.26 no.6
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• pp.646-650
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• 2016

The influence of chromosome number on cell growth and cell aging was investigated in various yeast strains that have many artificial chromosomes constructed using a chromosome manipulation technique. Host strain FY833 and the YKY18, YKY18R, YKY24, and YKY30 strains harboring 16 natural chromosomes, 18 chromosomes, 18 chromosomes containing rDNA chromosome, 24 chromosomes, and 30 chromosomes, respectively, were used, and the specific growth rate of each strain was compared. The specific growth rates in the YKY18 and YKY24 strains were indistinguishable from that in the host strain, while those of the YKY18R and YKY30 strains were reduced to approximately 25% and 40% of the host strain level, respectively. Subsequently, the replicative life span was examined to investigate the relationship between the number of chromosomes and cell aging, and the life span was decreased to approximately 14% and 45% of the host strain level in the YKY24 and YKY30 strains, respectively. Moreover, telomere length, well known as a senescence factor, was shorter and more diversified in the strain, showing decreased life span. Therefore, these results suggest the possibility that an increase in the number of chromosomes containing artificial chromosomes caused cell aging, and we expected these observations would be applied to improve industrial strain harboring of versatile and special artificial chromosomes.