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http://dx.doi.org/10.15230/SCSK.2013.39.4.313

Antioxidative and Anti-aging Effects of Extract from Talinum paniculatum  

Oh, Jung Young (R&D Center, Hanbul Cosmetics Co. Ltd.)
Kim, Jin Hwa (R&D Center, Hanbul Cosmetics Co. Ltd.)
Lee, Geun Soo (R&D Center, Hanbul Cosmetics Co. Ltd.)
Zhang, Yong He (Department of Pharmacology, School of Basic Medical Science, Peking University)
Pyo, Hyeong Bae (R&D Center, Hanbul Cosmetics Co. Ltd.)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.39, no.4, 2013 , pp. 313-322 More about this Journal
Abstract
Ultraviolet irradiation in the cells and skin produces reactive oxygen species (ROS), which induces the synthesis of matrix metalloproteinases (MMPs) causing skin photoaging. Using the human dermal fibroblast (HDF), we investigated the antioxidative and anti-aging effects of the extracts from Talinum paniculatum. Talinum paniculatum leaf and stem extracts (LSE) showed free radical scavenging effect by 98.45% at 500 ${\mu}g/mL$ and superoxide radical scavenging effect by 97.01% at 500 ${\mu}g/mL$ in the xanthine/xanthine oxidase system. The photoprotective potential of LSE was tested in HDF exposed to ultraviolet irradiation. It was revealed that LSE had an inhibitory effect on MMP-1 expression in UVA-irradiated HDF without any significant cytotoxicity. The treatment of UVA-irradiated HDF with LSE resulted in dose-dependent decreases in the expression levels of MMP-1 mRNA and protein. Also, UVB-induced cytotoxicity and cell death were effectively suppressed by treatment of LSE. Additionally, the senescence-associated ${\beta}$- galactosidase (SA-${\beta}$-gal) activity was decreased in the presence of LSE. These results suggest that Talinum paniculatum leaf and stem extracts (LSE) may have anti-aging effects and can be used as new functional materials against oxidative stress-mediated skin damages.
Keywords
Talinum paniculatum; antioxidation; MMP; anti-aging; ultraviolet;
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1 A. Oikarinen, The aging of skin: chronoaging versus photoaging, Photodermatol. Photoimmunol. Photomed., 7, 3 (1990).
2 B. A. Gilchrest, Skin aging and photoaging: an overview, J. Am. Acad. Dermatol., 21, 610 (1989).   DOI   ScienceOn
3 J. Uitto, M. J. Fazio, and D. R. Olsen, Molecular mechanism of cutaneous aging, J. Am. Acad. Dermatol., 21, 614 (1989).   DOI   ScienceOn
4 A. M. Klingman and R. M. Laver, Cutaneous aging: the differences between intrinsic aging and photoaging, J. Cutan. Aging. Cosmet. Dermatol., 1, 5 (1988).
5 S. K. Karin, B. Peter, W. Jutta, H. Gernot, M. Weijan, K. Lale, M. Christian, and W. Meinhard, Photoaging of the skin from phenotype to mechanisms, Experimen. Gerontol., 35, 307 (2000).   DOI   ScienceOn
6 J. Y. Seo, H. R. Choi, G. E. Rhie, C. S. Youn, W. W. Choi, J. A. Kim, J. H. Chung, K. H. Kim, K. H. Cho, and H. C. Eun, The effect of retinoic acid and vitamin C on the expression of the procollagen $\alpha$ 1(I), tropoelastin, and MMP-1 in human dermal fibroblast, Kor. J. Invest. Dermtaol., 8, 23 (2001).
7 C. Bailly, S. Dreze, D. Asselineau, B. Nusgens, C. M. Lapiere, and M. Darmon, Retinoic acid inhibits the production of collagenase by human epidermal keratinocytes, J. Invest. Dermatol., 94, 47 (1990).   DOI   ScienceOn
8 L. Hayflick and P. S. Moorhead, The serial cultivation of human diploid cell strains, Exp. Cell Res., 25, 585 (1961).   DOI   ScienceOn
9 G. P. Dimri, X. Lee, G. Basile, M. Acosta, G. Scott, C. Roskelley, E. E. Medrano, M. Linskens, I. Rubelj, O. Pereira-Smith, M. Peacocke, and J. Campisi, A biomarker that identifies senescent human cells in culture and in aging skin in vivo, Proc. Natl. Acad. Sci. U.S.A., 92, 9362 (1995).
10 D. B. Yarosh, S. Boumakis, A. B. Brown, M. T. Canning, J. W. Galvin, D. M. Both, E. Kraus, A. O'Connor, and D. A. Brown, Measurement of UVB-induced DNA damage and its consequences in models of immunosuppression, Methods, 28, 55 (2002).   DOI   ScienceOn
11 D. Kulms and T. Schwarz, Molecular mechanisms of UV-induced apoptosis, Photodermatol Photoimmunol Photomed., 16(5), 195 (2000).   DOI   ScienceOn
12 V. A. Smits and R. H. Medema, Checking out the G(2)/M transition, Biochim. Biophys. Acta., 1519, 1 (2001).   DOI   ScienceOn
13 J. Kim, C. W. Lee, E. K. Kim, S. J. Lee, N. H. Park, H. S. Kim, H. K. Kim, K. Char, Y. P. Jang, and J. W. Kim, Inhibition effect of Gynura procumbens extract on UVB-induced matrix-metalloproteinase expression in human dermal fibroblasts, J. Ethnopharmacol, 137, 427 (2011).   DOI   ScienceOn
14 J. Y. Bae, J. S. Choi, Y. J. Choi, S. Y. Shin, S. W. Kang, S. J. Han, and Y. H. Kang, (-)Epigallocatechin gallate hampers collagen destruction and collagenase activation in ultraviolet-B-irradiated human dermal fibroblasts: involvement of mitogen-activated protein kinase, Food Chem. Toxicol., 46, 1298 (2008).   DOI   ScienceOn
15 G. J. Fisher, S. C. Datta, H. S. Talwar, Z. Q. Wang, J. Varani, S. W. Kang, and J. J. Voorhees, Molecular basis of sun-induced premature skin ageing and retinoid antagonism, Nature, 379, 335 (1996).   DOI   ScienceOn
16 M. Komatsu, I. Yokoe, Y. Shirataki, and T. Tomimori, Studies on the constituents of Talinum paniculatum Gaertner. I, J. Pharm. Soc. Jap., 102, 499 (1982).   DOI
17 H. Shimoda, N. Nishida, K. Ninomiya, H. Matsuda, and M. Yoshikawa, Javaberine A, New TNF-$\alpha$ and nitric oxide production inhibitor, from the Roots of Talinum paniculatum, Heterocycles, 55, 2043 (2001).   DOI
18 T. Mosmann, Rapid colorimetric assay for the cellular growth and survival : application to proliferation and cytotoxic assay, J. Immun. Methods, 65, 55 (1983).   DOI   ScienceOn
19 M. S. Blois, Antioxidant determinations by the use of a stable free radical, Nature, 181, 1199 (1958).   DOI   ScienceOn
20 K. Furuno, T. Akasako, and N. Sugihara, The contribution of the pyrogallol moiety to the superoxide radical scavenging activity of flavonoids, Biol. Pharm. Bull., 25, 19 (2002).   DOI   ScienceOn
21 A. Kitahara, U. Matsumoto, H. Ueda, and R. Ueoka, A remarkable antioxidation effect of natural phenol derivatives on the autoxidation of $\gamma$-irradiated methyl linolate, Chem. Pharm. Bull., 40, 2208 (1992).   DOI
22 T. Hatano, Constituents of natural medicines with scavenging effects on ctive oxygen species-Tannins and related polyphenols, Natural Medicines, 49, 357 (1995).
23 P. Kuppusamy and J. L. Zweier, Characterization of free radical generation by xanthine oxidase, J. Biol. Chem., 264, 9880 (1989).
24 J. H. Chun, S. W. Kang, J. Varani, J. Lin, G. J. Fisher, and J. J. Voorhees, Decreased extracellular signal regulated kinase and increased stress activated MAP kinase activities in aged human skin in vivo, J. Invest. Dermatol., 115, 177 (2000).   DOI   ScienceOn
25 K. Scharffetter, M. Wlaschek, and A. Hogg, UVA irradiation induces collagenase in human dermal fibroblasts in vitro and in vivo, Arch. Dermatol. Res., 283, 506 (1991).   DOI   ScienceOn
26 M. Wlaschek, K. Bolsen, G. Herrmann, A. Schwarz, F. Wilmroth, P. C. Heinrich, G. Goerz, and K. Scharffetter, UVA induced autocrine stimulation of fibroblast derived collagenase by IL-6: a possilbe mechanism in dermal photodamage?, J. Invest. Dermatol., 101, 164 (1993).   DOI   ScienceOn
27 H. C. Kim, J. S. Yang, Y. S. Chae, K. S. Suh, and S. T. Kim, The effect of all-trans-retinoic acid and ursolic acid on the ultraviolet a radiation induced AP-1 (Fos/Jun) activity in cultured human dermal fibroblasts, Kor. J. Invest. Dermtaol., 35, 1136 (1997).
28 Y. W. Ryoo, S. I. Suh, K. C. Mun, B. C. Kim, and K. S. Lee, The effects of the melatonin on ultraviolet- B irradiated cultured dermal fibroblasts, J. Dermatol. Science, 27, 162 (2001).   DOI   ScienceOn
29 M. Barthelman, W. B. Bair, K. K. Stickland, W. Chen, B. N. Timmermann, S. Valcic, Z. Dong, and G. T. Bowden, (-)-epigallocatechin-3-gallate inhibition of ultraviolet B-induced AP-1 activity, Carcinogenesis, 19, 2201 (1998).   DOI   ScienceOn
30 G. J. Fisher, H. S. Talwar, J. Lin, P. Lin, P. McPhillips, Z. Q. Wang, X. Li, Y. Wan, S. W. Kang, and J. J Voorhees, Retinoic acid inhibits induction of c-Jun protein by ultraviolet radiation that occurs subsequent to activation of mitogen-activated protein kinase pathways in human skin in vivo, J. Clin. Invest., 101, 1432 (1998).   DOI   ScienceOn