• Title/Summary/Keyword: 세대 연구

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Development of Marker-free Transgenic Rice Expressing the Wheat Storage Protein, Glu-1Dy10, for Increasing Quality Processing of Bread and Noodles (빵과 면의 가공적성 증진을 위한 밀 저장단백질 Glu-1Dy10을 발현하는 마커프리 형질전환 벼 개발)

  • Park, Soo-Kwon;Shin, DongJin;Hwang, Woon-Ha;Hur, Yeon-Jae;Kim, Tae-Heon;Oh, Se-Yun;Cho, Jun-Hyun;Han, Sang-Ik;Lee, Seung-Sik;Nam, Min-Hee;Park, Dong-Soo
    • Journal of Life Science
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    • v.24 no.6
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    • pp.618-625
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    • 2014
  • Rice flour is used in many food products. However, dough made from rice lacks extensibility and elasticity, making it less suitable than wheat for many food products such as bread and noodles. The high-molecular weight glutenin subunits (HMW-GS) of wheat play a crucial role in determining the processing properties of the wheat grain. This paper describes the development of marker-free transgenic rice plants expressing a wheat Glu-Dy10 gene encoding the HMG-GS from the Korean wheat cultivar 'Jokyeong' using Agrobacterium-mediated co-transformation. Two expression cassettes, consisting of separate DNA fragments containing Glu-1Dy10 and hygromycin phosphotransferase II (HPTII) resistance genes, were introduced separately into Agrobacterium tumefaciens EHA105 for co-infection. Each EHA105 strain harboring Glu-1Dy10 or HPTII was infected into rice calli at a 3: 1 ratio of Glu-1Bx7 and HPTII. Among 290 hygromycin-resistant $T_0$ plants, we obtained 29 transgenic lines with both the Glu-1Dy10 and HPTII genes inserted into the rice genome. We reconfirmed the integration of the Glu-1Dy10 gene into the rice genome by Southern blot analysis. Transcripts and proteins of the Glu-1Dy10 in transgenic rice seeds were examined by semi-quantitative RT-PCR and Western blot analysis. The marker-free plants containing only the Glu-1Dy10 gene were successfully screened in the $T_1$ generation.

Variation in Pod Shattering in a RIL Population and Selection for Pod Shattering Tolerance in Soybean [Glycine max (L.) Merr] (콩 RIL 집단의 내탈립성 변이 탐색 및 유망계통 선발)

  • Seo, Jeong Hyun;Kang, Beom Kyu;Kim, Hyun Tae;Kim, Hong Sik;Choi, Man Soo;Oh, Jae Hyeon;Shin, Sang Ouk;Baek, In Youl;Kwak, Do Yeon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.64 no.4
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    • pp.414-421
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    • 2019
  • Pod shattering during the maturing stage causes a serious yield loss in soybean. It is the main limiting factor of soybean cultivation and mechanization. It is important to develop varieties suitable for mechanical harvesting and to develop energy-efficient agricultural machinery to save labor and costs. 'Daewonkong,' developed by the National Institute of Crop Science (NICS) in 1997, is an elite cultivar that occupies more than 80% of the soybean cultivation area in Korea because of its strong tolerance to pod shattering. The objectives of this study were to investigate the variation in pod shattering degree in a RIL population developed from a 'Daewonkong' parent and to select promising lines with pod shattering tolerance. 'Daewonkong' demonstrated a high level of tolerance to pod shattering compared to the 'Tawonkong' and 'Saeolkong' varieties, with no shattered pods after 72 hours of drying. Screening of pod shattering showed a clear distinction between the tolerant and susceptible varieties. Also, the distribution of shattering pod ratio in the two populations showed a similar pattern for three years. The promising lines with pod shattering tolerance included 27 lines in the 'Daewonkong'×'Tawonkong' population and 21 lines in the 'Daewonkong'×'Saeolkong' population. The promising lines are expected to be widely used as breeding parents for creating soybean cultivars with pod shattering tolerance.

A Hardware Implementation of the Underlying Field Arithmetic Processor based on Optimized Unit Operation Components for Elliptic Curve Cryptosystems (타원곡선을 암호시스템에 사용되는 최적단위 연산항을 기반으로 한 기저체 연산기의 하드웨어 구현)

  • Jo, Seong-Je;Kwon, Yong-Jin
    • Journal of KIISE:Computing Practices and Letters
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    • v.8 no.1
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    • pp.88-95
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    • 2002
  • In recent years, the security of hardware and software systems is one of the most essential factor of our safe network community. As elliptic Curve Cryptosystems proposed by N. Koblitz and V. Miller independently in 1985, require fewer bits for the same security as the existing cryptosystems, for example RSA, there is a net reduction in cost size, and time. In this thesis, we propose an efficient hardware architecture of underlying field arithmetic processor for Elliptic Curve Cryptosystems, and a very useful method for implementing the architecture, especially multiplicative inverse operator over GF$GF (2^m)$ onto FPGA and futhermore VLSI, where the method is based on optimized unit operation components. We optimize the arithmetic processor for speed so that it has a resonable number of gates to implement. The proposed architecture could be applied to any finite field $F_{2m}$. According to the simulation result, though the number of gates are increased by a factor of 8.8, the multiplication speed We optimize the arithmetic processor for speed so that it has a resonable number of gates to implement. The proposed architecture could be applied to any finite field $F_{2m}$. According to the simulation result, though the number of gates are increased by a factor of 8.8, the multiplication speed and inversion speed has been improved 150 times, 480 times respectively compared with the thesis presented by Sarwono Sutikno et al. [7]. The designed underlying arithmetic processor can be also applied for implementing other crypto-processor and various finite field applications.

Estimation of Breeding Values for Economic Traits in Korean Native Chicken (한국재래닭의 경제 형질에 대한 육종가 추정)

  • Sang B. D.;Choi C. H.;Kim H. K.;Kim S. D.;Jang B. G.;Na J. C.;Yu D. J.;Lee S. J.;Sang B. C.;Lee J. H.
    • Korean Journal of Poultry Science
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    • v.32 no.4
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    • pp.231-237
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    • 2005
  • This study was carried out to evaluate the breeding values for economic traits in Korean native chicken on the basis of 11,583 birds of record along 7 generations collected from 1995 to 2001 at National Livestock Research Institute, Korea. The results obtained are summarized as follows : The breeding value estimates of the body weights at 150 and 270 days in Dark Brown, Light Brown, Gray Brown, Black and White strains were $-16.563\~0.474\;and\;-18.836\~21.700,\;-14.714\~9.272\;and\;-23.025\~11.297,\; -25.186\~12.953\;and\;-28.368\~18.156,\;-15.549\~6.329\;and\;-18.264\~4.963,\;-10.708\~20.284\; and\;-25.112\~10.132g$, respectively. The breeding value estimates of the ages at first egg in Dark Brown, Light Brown, Gray Brown, Black and White strains were $-1.236\~0.047,\;-1.509\~0.298,\;-1.362\~0.114,\;-1.436\~0.199\;and\;-1.385\~0.718$, respectively. The breeding value estimates of the egg weight at first egg and at 270 days were $-0.105\~0.075\;and\;-0.120\~0.398,\;-0.109\~0.042\;and\;-0.205\~0.173,\;-0.166\~0.084\;and\;-0.256\~0.091,\;-0.119\~0.066\;and\;-0.051\~0.132,\;-0.112\~0.027\;and\;-0.070\~0.101g$, respectively. The breeding value estimates of the egg Production to 270 days in Dark Brown, Light Brown, Gray Brown, Black and White strains were $0.298\~l.807,\;-0.049\~l.146,\;-0.396\~l.125,\;-1.206\~l.173\;and\;-0.474\~2.641$, respectively.

A Study on the Insurance Contribution and Health Care Utilization of the Regional Medical Insurance Scheme (1개 군지역 의료보험제도에서의 보험료 부담수준별 병.의원 의료이용에 관한 연구)

  • Lee, Sang-Il;Choi, Hyun-Rim;Ahn, Hyeong-Sik;Kim, Yong-Ik;Shin, Young-Soo
    • Journal of Preventive Medicine and Public Health
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    • v.22 no.4 s.28
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    • pp.578-590
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    • 1989
  • This study was conducted to assess the equity in the regional insurance scheme through analysis of the computerized data from one regional insurance society and National Federation of Medical Insurance. We analysed the insurance contribution and benefit by the classes based on total and income-related contribution per household. The major findings of this study are as follows : 1. The average proportion of income-related contribution among the total was 39.2% and the upper classes show higher proportion of the income-related contribution. 2. The upper classes show higher health care utilization rate than the lower classes. It suggests that the lower classes have relatively large unmet medical needs. 3. The analysis through the Lorenz curve reveals that there exists transference of contributions from the upper to lower classes. But the cumulative percentage of insurance benefit is smaller than that of the number of the insured. It implies that regional medical insurance scheme in Korea has still some inequity in the context of social security principles.

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The Correlations of Parameters Using Contrast Enhanced Ultrasonography in the Evaluation of Prostate Cancer Angiogenesis (전립선암쥐모형의 신생혈관생성의 평가를 위해 시행된 역동적 조영 증강 초음파에서 얻은 변수간의 상관성연구)

  • Hwang, Sung Il;Lee, Hak Jong;Kim, Kil Joong;Chung, Jin-haeng;Jung, Hyun Sook;Jeon, Jong June
    • Ultrasonography
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    • v.32 no.2
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    • pp.132-142
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    • 2013
  • Purpose: The purpose of this study is to investigate the correlations of various kinetic parameters derived from the time intensity curve in a xenograft mouse model injected with a prostate cancer model (PC-3 and LNCaP) using an ultrasound contrast agent with histopathologic parameters. Materials and Methods: Twenty nude mice were injected with human prostate cancer cells (15 PC-3 and five LNCaP) on their hind limbs. A bolus of $500{\mu}L$ ($1{\times}10^8$ microbubbles) of second-generation US contrast agent (SonoVue) was injected into the retroorbital vein. The region of interest was drawn over the entire tumor. The time intensity curve was acquired and then fitted to a gamma variate function. The maximal intensity (A), time to peak (Tp), maximal wash-in rate (washin), washout rate (washout), area under the curve up to 50 sec ($AUC_{50}$), area under the ascending slope ($AUC_{in}$), and area under the descending slope ($AUC_{out}$) were derived from the parameters of the gamma variate fit. Immunohistochemical staining for VEGF and CD31 was performed. Tumor volume, the area percentage of VEGF stained in a field, and the count of CD31 (microvessel density, MVD) positive vessels showed correlation with the parameters from the time intensity curve. Results: No significant differences were observed between the kinetic and histopathological parameters from each group. MVD showed positive correlation with A (r=0.625, p=0.003), washin (r=0.462, p=0.040), $AUC_{50}$ (r=0.604, p=0.005), and $AUC_{out}$ (r=0.587, p=0.007). Positive correlations were also observed between tumor volume and $AUC_{50}$ (r=0.481, p=0.032), washin (r=0.662, p=0.001), and $AUC_{out}$ (r=0.547, p=0.012). Washout showed negative correlations with MVD (r=-0.454, p=0.044) and tumor volume (r=-0.464, p=0.039). The area percentage of VEGF did not show any correlation with calculated data from the curve. Conclusion: MVD showed correlations with several of the kinetic parameters. CEUS has the potential for prediction of tumor vascularity in a prostate cancer animal model.

Effects and Roles of Korean Community Dance (한국 커뮤니티 댄스의 효과와 역할)

  • Park, Sojung
    • Trans-
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    • v.9
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    • pp.37-66
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    • 2020
  • Entering the 21st century, the flow of society and culture is emerging as a cultural phenomenon in which one experiences, enjoys, and experiences on one's own. This trend has emerged as community dance, which has been active since 2010. Community dances can be targeted by anyone and can be divided into children's, adult and senior citizens' dances depending on the characteristics and age of the group, allowing them to work in various age groups. It also refers to all kinds of dances for the happiness and self-achievement of everyone who can promote gender, race and religion health or meet the needs of expression and improve their physical strength at meetings by age group, from preschoolers to senior citizens. Community dance is a dance activity in which everyone takes advantage of their leisure time and voluntarily participates in joyous activities, making it expandable to lifelong education and social learning. It is a voluntary community gathering conducted by experts for the general public. The definition of community dance can be said to be the aggregate of physical activities that enrich an individual's daily life and enhance their social sense to create a bright society, while individuals achieve the goals of health promotion and aesthetic education. In the contemporary community dance, the dance experience in body and creativity as self-expression reflects the happiness perspective by exploring the positive psychological experience and influence of the participants in the process of participation, and participants have continued networking through online offline to enjoy the dance culture. Although research has been conducted in various fields for 10 years since the boom in community dance began, the actual methodology of the program has been insufficient to present the Feldenkrais Method, hoping that it will be used as a methodology necessary for local community dance, and will be used as part of the educational effects and choreography creation methods of artists that can improve the physical functional aspects of dance and give a sense of psychological stability.

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Mechanical Property Evaluation of Dielectric Thin Films for Flexible Displays using Organic Nano-Support-Layer (유기 나노 보강층을 활용한 유연 디스플레이용 절연막의 기계적 물성 평가)

  • Oh, Seung Jin;Ma, Boo Soo;Yang, Chanhee;Song, Myoung;Kim, Taek-Soo
    • Journal of the Microelectronics and Packaging Society
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    • v.28 no.3
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    • pp.33-38
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    • 2021
  • Recently, rollable and foldable displays are attracting great attention in the flexible display market due to their excellent form factor. To predict and prevent the mechanical failure of the display panels, it is essential to accurately understand the mechanical properties of brittle SiNx thin films, which have been used as an insulating film in flexible displays. In this study, tensile properties of the ~130 nm- and ~320 nm-thick SiNx thin films were successfully measured by coating a ~190 nm-thick organic nano-support-layer (PMMA, PS, P3HT) on the fragile SiNx thin films and stretching the films as a bilayer state. Young's modulus values of the ~130 nm and ~320 nm SiNx thin films fabricated through the controlled chamber pressure and deposition power (A: 1250 mTorr, 450 W/B: 1000 mTorr, 600 W/C: 750 mTorr, 700 W) were calculated as A: 76.6±3.5, B: 85.8±4.6, C: 117.4±6.5 GPa and A: 100.1±12.9, B: 117.9±9.7, C: 159.6 GPa, respectively. As a result, Young's modulus of ~320 nm SiNx thin films fabricated through the same deposition condition increased compared to the ~130 nm SiNx thin films. The tensile testing method using the organic nano-support-layer was effective in the precise measurement of the mechanical properties of the brittle thin films. The method developed in this study can contribute to the robust design of the rollable and foldable displays by enabling quantitative measurement of mechanical properties of fragile thin films for flexible displays.

A Study on the Consciousness of Landscape Planting Practitioner about the Measurement Criteria of the Root Diameter of Landscape Trees in the Landscape Planting Construction, in Korea (우리나라 조경식재공사의 근원직경 측정기준에 대한 조경식재 실무자들의 의식)

  • Han, Yong-Hee;Min, Jong-Il;Kim, Do-Gyun
    • Journal of the Korean Institute of Landscape Architecture
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    • v.49 no.2
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    • pp.27-40
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    • 2021
  • This study was carried out for the description of the conflicts on the measurement of the root collar diameter of the landscape trees that are currently being produced, distributed, and planted in S. Korea, and for determination of the standard for root collar diameter measurement. The difference in consciousness of appropriate measurement of root collar diameter among different ages of landscape practitioners was statistically significant at p<0.05 level. It seemed to be due to the difference in the amount of field experiences among different age groups. On "the ambiguity of measuring the root collar diameter' of landscape trees", the consciousness was significantly different at p<0.05 level among job positions. On "Improvement of measurement criteria for landscape trees," it was significantly different at p<0.05 level among job types. This was thought to be due to the disagreement between the client and the contractor. On "prevention of topsoil removal" when excavating landscape trees, the consciousness was significantly different at p<0.001 level among different age groups, and different at p<0.01 level among different occupations, and different at p<0.05 level among different working area. The consciousness on "removing top soil when excavating landscape trees and rooting after transplantation" was not significantly different. The consciousness on the conflict caused by "ambiguity in root collar diameter measurement criteria" was high with an average of 3.85 for job type, occupation, jop position, and work experience. It was higher for landscape contractors than public institutions. The higher job positions and more experiences, the more conflicts. The consciousness on the appropriate position of root collar diameter measurement for landscape trees revealed that measuring at above-ground part (66.5%) was prefered to the underground part (33.0%). During the excavation of landscape trees for transplant, topsoil removal up to average depth of -2cm to -4cm was favored by 84.0%, and the purpose of removing topsoil was recognized as 'to increase the size and unit cost' by 59.7%.

A Comparative Errors Assessment Between Surface Albedo Products of COMS/MI and GK-2A/AMI (천리안위성 1·2A호 지표면 알베도 상호 오차 분석 및 비교검증)

  • Woo, Jongho;Choi, Sungwon;Jin, Donghyun;Seong, Noh-hun;Jung, Daeseong;Sim, Suyoung;Byeon, Yugyeong;Jeon, Uujin;Sohn, Eunha;Han, Kyung-Soo
    • Korean Journal of Remote Sensing
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    • v.37 no.6_1
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    • pp.1767-1772
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    • 2021
  • Global satellite observation surface albedo data over a long period of time are actively used to monitor changes in the global climate and environment, and their utilization and importance are great. Through the generational shift of geostationary satellites COMS (Communication, Ocean and Meteorological Satellite)/MI (Meteorological Imager sensor) and GK-2A (GEO-KOMPSAT-2A)/AMI (Advanced Meteorological Imager sensor), it is possible to continuously secure surface albedo outputs. However, the surface albedo outputs of COMS/MI and GK-2A/AMI differ between outputs due to Differences in retrieval algorithms. Therefore, in order to expand the retrieval period of the surface albedo of COMS/MI and GK-2A/AMI to secure continuous climate change monitoring linkage, the analysis of the two satellite outputs and errors should be preceded. In this study, error characteristics were analyzed by performing comparative analysis with ground observation data AERONET (Aerosol Robotic Network) and other satellite data GLASS (Global Land Surface Satellite) for the overlapping period of COMS/MI and GK-2A/AMI surface albedo data. As a result of error analysis, it was confirmed that the RMSE of COMS/MI was 0.043, higher than the RMSE of GK-2A/AMI, 0.015. In addition, compared to other satellite (GLASS) data, the RMSE of COMS/MI was 0.029, slightly lower than that of GK-2A/AMI 0.038. When understanding these error characteristics and using COMS/MI and GK-2A/AMI's surface albedo data, it will be possible to actively utilize them for long-term climate change monitoring.