• Title/Summary/Keyword: 세대 연구

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Stable expression of brazzein protein, a new type of alternative sweetener in transgenic rice (형질전환 벼에서 brazzein 감미단백질의 안정적인 발현)

  • Lee, Ye Rim;Akter, Shahina;Lee, In Hye;Jung, Yeo Jin;Park, So Young;Cho, Yong-Gu;Kang, Kwon Kyoo;Jung, Yu Jin
    • Journal of Plant Biotechnology
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    • v.45 no.1
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    • pp.63-70
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    • 2018
  • Brazzein is the smallest sweet protein and was isolated from the fruit pulp of Pentadiplandra brazzeana Baillon, native to tropical Africa. From ancient times, the indigenous people used this fruit in their diet to add sweetness to their daily food. Brazzein is 500 to 2000 times sweeter than sucrose on a weight basis and 9500 times sweeter on a molar basis. This unique property has led to increasing interest in this protein. However, it is expensive and difficult to produce brazzein other than in its native growing conditions which limits its availability for use as a food additive. In this study, we report high production yields of, brazzein protein in transgenic rice plants. An ORF region encoding brazzein and driven by the $2{\times}CaMV\;35S$ promoter was introduced into rice genome (Oryza sativa Japonica) via Agrobacterium-mediated transformation. After transformation, 17 regenerated plant lines were obtained and these transgene-containing plants were confirmed by PCR analysis. In addition, the selected plant lines were analyzed by Taqman PCR and results showed that 9 T0 lines were found to have a single copy out of 17 transgenic plants. Moreover, high and genetically stable expression of brazzein was confirmed by western blot analysis. These results demonstrate that recombinant brazzein was efficiently expressed in transgenic rice plants, and that we have developed a new rice variety with a natural sweetener.

A Study on Enhancing VMS Services by FM Car Radio (차량 내 FM라디오를 이용한 VMS서비스 개선 연구)

  • Park, Bum-Jin;Moon, Byeong-Sup
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.9 no.6
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    • pp.22-32
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    • 2010
  • Increasingly advanced Information Technology (IT) has changed the operator so as to create more diversified and advanced traffic information demand. To deal with the changing demand in private sector, a concept of on-demand traffic information has been rapidly introduced. However VMS, a product of the first generation of ITS, which was designed to provide the unspecified individuals during driving the car with the basic level of traffic information by the public failed to actively change itself in such a changing pattern. This study was intended to describe the VMS system (tentatively, FM-VMS) which was further developed to accommodate the needs favoring the sophisticated PDA with the public role of providing the unspecified individuals with the equal information. FM-VMS introduced in this study is the device designed to transmit the voice and message to the drivers through the radio information device mounted on a car. A core technology is, unlike FM-DARC and RDS, the Water Making technology which directly inserts the digital signal into FM frequency in use. It's been currently used for broadcasting and security purpose. A detection rate as a result of testing FM-VMS system using Water Making technology was 90% or more in voice and message within 20m from test VMS. When a public-developed VMS information could be transmitted using FM frequency to the relatively vulnerable users (vulnerable to traffic information) in voice on a real-time basis to provide the regional traffic information, and furthermore, VMS message could be received through radio liquid using FM frequency only, it would obviously bring about the innovation in ITS as well as pave the way for creating the new added value down the road.

Information Analyses of Child Who Needs Protection in Busan (요보호아동의 정보화 실태 분석: 부산지역을 중심으로)

  • 김옥희
    • Journal of Families and Better Life
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    • v.20 no.5
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    • pp.149-166
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    • 2002
  • In this study, 1 have analyzed the state of the use of information technology by the at-risk children who are residing in Husan metropolitan area. The ultimate goal of this study is to help ensure the healthy development of the children and improve the quality of their lives in this information-oriented society. For this research, 1 surveyed 183 children living in an institution or a low-income family in Busan metropolitan area. The survey questionnaire was designed to obtain information on the respondents, socio-demographic characteristics, computer-related conditions of the immediate and social environments, patterns of computer and Internet use, their experience and degree of so called, Net-generation syndrome, Internet addiction disorder, changes in social and familial life caused by Internet use, and exposure to pornographic materials. The results show that 95.9 % of at-risk children in Husan own a computer, 60.9 % of those computers being Pentium Ⅲ. A majority of the children (74.9 %) are connected to Internet at a high-speed, using such devices as LAN, ADSL or cables. About seventy-six percent of the children have used a PC and Internet less than two years. In other words, a majority of them have not used those for a long period of time. The main results of the research are summarized in the following: There is a significant difference among at-risk children in terms of experiences in computer and Internet use according to their grade levels. There is a gender difference in the amount of time spent on computer and Internet. Boys spend more time every week on computer and Internet than girls do. There is a significant difference in the pattern of computer and Internet use, according to the type of their risk, and grade level. The so-called 'Net Generation Syndrome' is not serious in this group. A majority of the at-risk children think that Internet is more interesting than TV. They hardly shop on Internet, and they don think the advertisements on Internet are a serious problem. Also, unlike many people assumptions, their desire to communicate through on-line chatting was very low. They do not tend to buy computer game programs, and Internet Addiction Disorder was not pronounced among them. The at-risk children rarely had an exposure to pornographic materials in the cyber space. However, the exposure was more frequent for institutionalized children than children in low-income families. These results point to a need for a policy to improve the welfare of at-risk children and guarantee their right to information.

Evaluation and Improvement Measures on collection and transportation System of Food Waste (음식물 쓰레기 수집운반체계 평가 및 개선 방안)

  • Lee, Hyun-Hee;Ryu, Ji-Young;Shin, Dae-Yewn;Phae, Chae-Gun
    • Journal of the Korea Organic Resources Recycling Association
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    • v.12 no.2
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    • pp.110-120
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    • 2004
  • This research sought to analyze the food waste collection and transportation system in Korea's related public resource-making facilities, address related problems, and suggest a reasonable alternative measure. For the food waste sorting-out and discharging methods, apartments and similar housings use containers, and individual-style housings use 'quantity-proportional' bags. The sorting and discharging methods should be improved to boost the reuse of resource-made output. The analysis of collection and transportation costs by local governments indicates that they range from 30,000 won to 150,000 won per ton, varying greatly by characteristics of local governments, thus suggesting a need to establish costs calculation criteria to justify the costs. Charges imposed to each household were not greatly problematic in the case of quantity-proportional bags; however, in the case of apartments and similar housings, charges varied greatly, suggesting a need to impose differentiated charges calculated on the basis of collection and transportation costs according to regions. Many places used household-garbage collection vehicles to collect food waste, raising the possibility of causing complaints on leaching water and bad odors. Thus, exclusive food waste collection vehicles should be gradually introduced. Also, in certain places, vehicles should travel more than 20km to collect food waste, and in such cases, a cargo transferring system should be introduced to boost efficiency in carriage.

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Studies on the Parasitoids of Chestnut Gall Wasp, Dryocosmus kuriphilus Yasumatsu (Hymenoptera : Cynipidae) in Korea (밤나무혹벌의 기생천적(寄生天敵)에 관(關)한 연구(硏究))

  • Kim, Jong Kuk
    • Journal of Korean Society of Forest Science
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    • v.87 no.3
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    • pp.475-482
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    • 1998
  • 9 species of parasitoids emerged from chestnut gall samples collected at Chunchon, Kangwon in June to July 1995 - 1997, and 8 species emerged from gall samples at the 5 investigation sites in December 1997. The primary parasitoids among them were Torymus sinensis, Torymus geranii, Sycophila variegata, Megastigmus nipponicus, Megastigmus maculipennis, Eurytoma brunniventris, Ormyrus flavitibialis, Ormyrus punctiger, Eupelmus urozonus, while the secondary parasitoids were Torymus geranii, Sycophila variegata, Eupelmus urozonus, Eupelmus sp., Eurytoma setigera, Ormyrus punctiger. Torymus sp.. Some of secondary parasitoids were facultative hyperparasitoids, parasitizing the gall wasp as well as primary parasitoids, Among these natural enemies, Torymus sinensis was dominant at all sites. At Chunchon, the rate of gall formation showed decreasing tendency as the function of generation and the parasitism rate of Torymus sinensis showed increasing tendency gradually. But the rate of parasitization of the secondary parasitoids(Sycophila variegata, Eupelmus urozonus, Torymus geranii, Ormyrus puntiger) ranged from 4.0% to 10.5%. The mortality was high during June to October which was immature stage of Torymus sinensis, and mainly caused by secondary parasitoids. Torymus sinensis emerged from April to May and the estimated dates of 50% emergence was 21st April. The estimated dates of 50% emergence for the other, Sycophila variegata, Eupelmus urozonus, Torymus geranii, and Ormyrus puntiger were 25th April, 30th April, 4th May, and 22nd May, respectively.

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An Efficient Parallelization Implementation of PU-level ME for Fast HEVC Encoding (고속 HEVC 부호화를 위한 효율적인 PU레벨 움직임예측 병렬화 구현)

  • Park, Soobin;Choi, Kiho;Park, Sang-Hyo;Jang, Euee Seon
    • Journal of Broadcast Engineering
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    • v.18 no.2
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    • pp.178-184
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    • 2013
  • In this paper, we propose an efficient parallelization technique of PU-level motion estimation (ME) in the next generation video coding standard, high efficiency video coding (HEVC) to reduce the time complexity of video encoding. It is difficult to encode video in real-time because ME has significant complexity (i.e., 80 percent at the encoder). In order to solve this problem, various techniques have been studied, and among them is the parallelization, which is carefully concerned in algorithm-level ME design. In this regard, merge estimation method using merge estimation region (MER) that enables ME to be designed in parallel has been proposed; but, parallel ME based on MER has still unconsidered problems to be implemented ideally in HEVC test model (HM). Therefore, we propose two strategies to implement stable parallel ME using MER in HM. Through experimental results, the excellence of our proposed methods is shown; the encoding time using the proposed method is reduced by 25.64 percent on average of that of HM which uses sequential ME.

The Effects of GyeongIn Ara Waterway on the Regional Property Value (경인아라뱃길이 지역 부동산 가격에 미친 영향 분석)

  • Lee, Hee-Chan;Cha, Joo-Young;Park, Doo-Ho
    • Journal of Korea Water Resources Association
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    • v.46 no.3
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    • pp.277-285
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    • 2013
  • The purpose of this research is to evaluate the scenic value of the Gyungin Ara waterway in real estate prices. Apart from the multi-functionality such as transportation of passengers and freight, prevention of floods, and provision of leisure areas, the Ara waterway possesses a scenic function which offers people esthetic value through unique and beautiful scenery. This scenic function is an externality for apartment residents living nearby. The applied methodology for this research is the Hedonic Price Model (HPM) which creates a cause and effect model between real estate prices and attributes. Variables such as apartment sale prices, complex characteristics, location characteristics, timely characteristics have been deduced through data collected from a total of 4,207 households that have experienced actual transactions during the same period, all located within the scenic benefit boundaries of the waterway. Landscape variable has been derived from algorithm designed by a combination of digital map and Google Mapview. The scenic value of the waterway estimated through the application of HPM on these variables is 165,000 Won per area (pyeong). The regional asset enhancing effect caused by the landscape view of the waterway is estimated to be 89.1 billion won.

Study of Treatment Methods on Solution-Processed ZnSnO Thin-Film Transistors for Resolving Aging Dynamics

  • Jo, Gwang-Won;Baek, Il-Jin;Jo, Won-Ju
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.348-348
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    • 2014
  • 차세대 디스플레이 구동 회로 소자를 위한 재료로서, Amorphous Oxide Semiconductor (AOS)가 주목받고 있다. AOS는 기존의 Amorphous Silicon과 비교하여 뛰어난 이동도를 가지고 있으며, 넓은 밴드 갭에 의한 투명한 광학적 특성을 가지고 있다. 이러한 장점을 이용하여, AOS 박막은 thin film transistor (TFT)의 active channel로 이용 되고 있다. 하지만, AOS를 이용한 TFT의 경우, 시간이 경과함에 따라 $O_2$$H_2O$ 흡착에 의해 전기적 특성이 변하는 현상이 있다. 이러한 현상은 소자의 신뢰성에 있어 중요한 문제가 된다. 이러한 문제를 연구하기 위해 본 논문에서는, AOS 박막을 이용하여 bottom 게이트형 TFT를 제작하였다. 이를 위해 먼저, p-type Si 위에 건식산화방식으로 $SiO_2$(100 nm)를 성장시켜 게이트 산화막으로 이용하였다. 그리고 Zn과 Sn이 1: 2의 조성비를 가진 ZnSnO (ZTO) 용액을 제조한 후, 게이트 산화막 위에 spin coating 하였다. Splin coating된 용액에 남아 있는 솔벤트를 제거하기 위해 10분 동안 $230^{\circ}C$로 열처리를 한 후, 포토리소그래피와 에칭 공정을 이용하여 ZTO active channel을 형성하였다. 그 후, 박막 내에 남아 있는 불순물을 제거하고 ZTO TFT의 전기적인 특성을 향상시키기 위하여, $600^{\circ}C$의 열처리를 30분 동안 진행 하여 junctionless형 TFT 제작을 완료 하였다. 제작된 소자의 시간 경과에 따른 열화를 확인하기 위하여, 대기 중에서 2시간마다 HP-4156B 장비를 이용하여 전기적인 특성을 확인 하였으며, 이러한 열화는 후처리 공정을 통하여 회복시킬 수 있었다. 열화의 회복을 위한 후처리 공정으로, 퍼니스를 이용한 고온에서의 열처리와 microwave를 이용하여 저온 처리를 이용하였다. 결과적으로, TFT는 소자가 제작된 이후, 시간에 경과함에 따라서 on/off ratio가 감소하여 열화되는 경향을 보여 주었다. 이러한 현상은, TFT 소자의 ZTO back-channel에 대기 중에 있는 $O_2$$H_2O$의 분자의 물리적인 흡착으로 인한 것으로 보인다. 그리고 추가적인 후처리 공정들에 통해서, 다시 on/off ratio가 회복 되는 현상을 확인 하였다. 이러한 추가적인 후처리 공정은, 열화된 소자에 퍼니스에 의한 고온에서의 장시간 열처리, microwave를 이용한 저온에서 장시간 열처리, 그리고 microwave를 이용한 저온에서의 단 시간 처리를 수행 하였으며, 모든 소자에서 성공적으로 열화 되었던 전기적 특성이 회복됨을 확인 할 수 있었다. 이러한 결과는, 저온임에도 불구하고, microwave를 이용함으로 인하여, 물리적으로 흡착된 $O_2$$H_2O$가 짧은 시간 안에 ZTO TFT의 back-channel로부터 탈착이 가능함과 동시에 소자의 특성을 회복 가능 함 의미한다.

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Web application firewall technology trends and testing methodology (웹방화벽 기술동향 파악 및 시험방법론)

  • Jo, In-june;Kim, Sun-young;Kim, Chan-joong
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2012.10a
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    • pp.132-138
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    • 2012
  • Existing network layer firewall security support is one that does not support the higher layer, the application layer of a vulnerable web application security. Under these circumstances, the vulnerability of web applications to be able to defend a Web Application Firewall is positioned as a solver to solve the important security issues of businesses spotlighted in the next generation of security systems, and a very active market in the market other than domestic is expected to be formed. However, Firewall Web has not yet proposed a standard which can be used to test the performance of the Web Application Firewall Web Application Firewall and select the products of trust hardly Companies in BMT conduct their own individual problems and the cost of performance testing technologies, there is a limit. In this study, practically usable BMT model was developed to evaluate the firewall vendor. Product ratings ISO / IEC 9126, eight product characteristics meet the performance and characteristics of a web application firewall entries are derived. This can relieve the burden on the need to be evaluated in its performance testing of Web firewall, and can enhance the competitiveness of domestic-related sectors, by restoring confidence in the product can reduce the dependence on foreign products.

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Study on deposition condition of epitaxial $Y_2O_3$ buffer layer deposited on textured metal substrates for $YBa_2Cu_3O_7$ coated conductors (YBCO Coated Conductor를 위한 texture된 금속 기판위의 epitaxial $Y_2O_3$ 완충층 증착 조건에 관한 연구)

  • Shin, K.C.;Ko, R.K.;Park, Y.M.;Chung, J.K.;Shi, Dongqi;Choi, S.J.;Song, K.J.;Park, C.;Son, Y.G.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2003.07a
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    • pp.565-568
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    • 2003
  • 2세대 초전도 선재로 알려져 있는 $YBa_2Cu_3O_{7-\delta}$ coated conductor는 금속모재/완충층/초전도층/보호층의 구조를 가진다. 2개 이상의 산화물 다층 박막으로 이루어진 완충층은 금속기판의 집합조직을 초전도층까지 전달하는 역할, 금속기판의 금속이 초전도층으로 확산되어 초전도층의 전기적 특성을 열화시키는 것을 막아주는 확산장벽으로의 역할 등을 수행한다. 1차 완충층은 금속기판의 집합조직을 유지하여야하며, 금속기판의 산화를 방지하면서 증착 되어야 한다. coated conductor 제조를 위한 첫 단계로 Pulsed Laser Deposition법을 이용하여 cube texture된 Ni 기판 위에 $Y_2O_3$ 박막을 증착 하였다. 최적의 증착 조건을 찾기 위해 증착 챔버의 산소 및 $H_2/Ar$ 혼합가스 분압과 기판온도를 변화시키면서 증착 하였다. $Y_2O_3$층의 (100) 집합조직은 기판온도 $600{\sim}700^{\circ}C$와 산소 분압 $0.01{\sim}0.1mTorr$에서 증착된 Y2O3 박막에서 금속기판과 유사한 집합조직을 얻을 수 있었다. 최적의 증착 조건에서 $Y_2O_3$ (222) ${\Phi}-scan$의 full width at half maximum (fwhm)이 $11^{\circ}$이고 (400) ${\omega}-scan$ fwhm은 $6^{\circ}$이었다.

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