• Korean Journal of Microbiology
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• v.37 no.4
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• pp.265-272
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• 2001

The diversity of acid-tolerant epiphytic bacterial communities on deciduous oak tree (Quercus dentate Thunb.) leaves was examined both in the natural forest area with a clean air and in the industrial estate to assess effects of acidic depositions to the phyllosphere using 16S rDNA sequence data. A total of 444 acid-tolerant epiphytic bacterial clones were obtained, resulting in 17 phylotypes by performing a analysis of restriction fragment length polymorphism (RFLP) for PCR-amplified 16S rDNA products. A very low diversity of dominating acid tolerant bacterial communities in both areas was found, just 2 subphyla groups, $\gamma$-Proteobacteria and low-G+C gram-positive bacteria. As tree leaves grow older, diversities of acid-tolerant bacteria on them significantly increased. The community structure of acid-tolerant epiphytic bacteria consisted of Pseudomonas and Enterobacteriaceae groups in the $\gamma$-Proteobacteria subphylum, and Streptococcaceae and Staphylococcus groups in the low-G+C gram-positive bacteria subphylum. The direct influence of acidic depositions on bacterial phylogenetic composition could not be detected especially when higher taxonomic levels such as subphylum, but at narrower or finer levels it could be observed by a detection of Xanthomonadales group belonged to the $\gamma$-Proteobacteria only in the industrial area and of Acetobacteraceae group belonged to the $\alpha$-Proteobacteria. There remains that these specific acid-tolerant epiphytic bacterial groups could be used as indicators for assessing effects of acidic depositions on the phyllosphere.

• Feed Journal
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• v.3 no.11 s.27
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• pp.118-123
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• 2005

"소의 질병 중에서 설사증은 송아지에서 가장 빈번히 발생하고 있으며, 전체 송아지 폐사 원인의 70$%$에 이를 정도로 막대한 경제적 피해를 입히고 있다. 송아지 설사증은 크게 바이러스성, 세균성, 원충성 질병에 의하며, 이 중에서 가장 문제를 일으키고 있는 것이 바이러스성 송아지 설사라 할 수 있다. 국내에서 잘 알려진 바이러스성 송아지 설사"

• Microbiology and Biotechnology Letters
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• v.21 no.1
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• pp.82-87
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• 1993

Fourteen samples of kimchies and soy bean pastes were used to isolate strictly anaerobic bacteria on complex BL agar and on a selective BS agar for bifidus bacteria. About $10^7$ ~ $10^8$ colonies per g sample were developed on BL agar under strictly anaerobic conditions, while BS agar supported the growth of $10^3$ ~ $10^6$ colonies per gram sample at the same condition. All colonies developed on BS agar at anaerobic conditions grew in aerobic conditions and did not show fructose6-phosphate phosphoketolase activity. Type cultures of Bifidobacterium did not grow in PYG medium containig more than 3% NaCI. From these results it is conduded that salted fermented food cannot support the growth of strictly anaerobes induding Bifidobactenum.

• Proceedings of the Korean Society of Veterinary Pathology Conference
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• 2001.09a
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• pp.23-23
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• 2001

대표적인 어류질병의 원인이 되는 주요 병원성 미생물인 에드워드균(Edwardsiella tarda), 비브리오균(Vibrio anguillarum), 연쇄구균(Streptococcus faecalis)의 방사선 감수성을 파악하고, 주요 생어사료재료 3종(고등어, 전어, 새우) 및 배합사료 1종을 대상으로 일반세균의 방사선 멸균 효과 및 생어사료에 병원성 세균을 인위적으로 오염시킨 후 방사선 조사에 의한 멸균 효과를 파악하였다. (중략)

• KOREAN POULTRY JOURNAL
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• v.49 no.5
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• pp.133-135
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• 2017

질적 양적 성장을 해온 양계산업이 최근에는 계분처리 등 악취문제로 인한 민원발생과 각종 소모성 및 전염성 질병으로 이중고를 겪고 있다. 이에, 주식회사 대자연(사장 신왕기, 이하 대자연)은 15년간의 연구 끝에 고전압과 특수합금을 활용한 고전압 미네랄이온활성수로 악취문제와 세균을 한방에 해결할 수 있는 시스템을 개발했다. 무독성으로 2차 오염물질을 생성하지 않고 세균이나 악취물질을 세정하거나 흡착시키는 임시방편적인 방법이 아니라 세균과 악취물질자체의 분자화학 결합을 깨트려 분해하는 근본적인 문제해결방법으로 음수로 먹이거나 분무로 뿌려주면 된다. 이번호에는 대자연을 소개코자 한다.

• Bulletin of Food Technology
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• v.23 no.4
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• pp.544-551
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• 2010

식품산업에서 박테리오파아지를 첫째, 동물 보건에서 항생제 대체품으로서, 둘째, 식품에서 바이오보존제(biopreservative)로서, 셋째, 식품 체인에서 병원성 세균을 검출하기위한 도구로서 다양하게 이용하고자 최근에 널리 인지하고 있다. 박테리오파아지는 바이러스로서 세균을 감염시키고 용해하는 특성이 있으며, 식품 안전과 관련하여, 1) 포유 동물세포에 무해하여 박테리오파아지를 안전하게 이용할 가능성, 2) 발효식품에 적절한 스타터 역할을 하거나 천연 미생물균총을 그대로 보존하는 박테리오파아지의 높은 숙주 선택성이라는 2가지 특징을 지니고 있다. 최근에는 식품첨가물로서 박테리오파아지를 '먹을 수 있는 바이러스'로 인정할 수 있을지 논의되고 있다. 식품유래 병원성 세균을 제어하기 위한 파아지의 이용가능성과 생물학 분야에서 관련되는 기초연구를 하고자 할 때 생기는 제한점 등에 대해서 고찰하고자 한다.

• Korean journal of applied entomology
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• v.23 no.2 s.59
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• pp.96-101
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• 1984

This experiment was carried out to investigate control efficacy by cultural practice and fumigation of tacterial wilt caused by Pseudomonas solancearum in resistant tobacco line NC82 at the Korea Ginseng and Tobacco Research Institute, Eumseong Experiment Station in 1982 and 1983. The bacterial wilt of tobacco occurred severely from mid-July to last August in applicable temperature and soil humidity for increasing bacteria. Disease severity appeared low and slowly at fumigation and resistant variety treatment. Incidence of bacterial wilt in tobacco line NC82 was $44.7\~55.8\%$ being compared with susceptible variety, NC2326 and $95\~99\%$ when resistant variety, NC32 was cultivated with soil fumigation treatment. Control efficacy of cultural practices appeared low with $0.8\~20\%$ and was not different from resistant variety and fumigation treatment. Control system against bacterial wilt in flue-cured tobacco was accomplished by control efficacy over $95\%$ when resistant variety(NC82) was cultured after treatment of cultural practices (Tillering after harvest and before transplanting, stalk and root destruction, early transplanting early removal of the mulching film) and soil fumigation(Cylon).

• Research in Plant Disease
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• v.6 no.1
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• pp.6-9
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• 2000

Nine samples of soft rotten roots and blighted leaves of Ficus spp. plants were collected from the vinyl-houses in Taejeon, Seongnam, Suweon and Yangjae in 1988 and pathogenic bactea were isolated from them Results of the studies on morphological, cultural, physiologucal and pathological characteristics indicated that the bacteria from Ficus retusa were Pseudomonas cichorii, from Ficus retusa \`Golden leaf\` and Ficus benjamina were P.viridiflava. These are the first description of bacteria which caused the diseases on Ficus spp. in Korea. We proposed to name the disease of Ficus retusa by P. cichorii as \"bacterial root rot of Ficus retusa\" and Ficus retusa(Golden leaf) and Ficus benjamina by P. viridiflava as \"bacterial leaf blight of Ficus retusa (Golden leaf)\", \"bacterial blight of Ficus benjamina\", respectively.

• KOREAN POULTRY JOURNAL
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• v.33 no.8 s.382
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• pp.121-125
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• 2001

건강한 닭은 병원성 미생물에 대하여 자연적인 저항성을 가지고 있다. 이것은 숙주상태와 장관사이에 서로 밀접한 협력장치가 있기 때문이며 건강한 병아리는 부화된 후 지속적으로 장관내에서 정상적인 세균총을 형성한다.

• The Journal of Dong Guk Oriental Medicine
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• v.7 no.2
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• pp.121-140
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• 1999

The various oriental herbal medicines, which have usually been used for treatment of reducing fever, purging intense heat and detoxication, were screened to determine the antibacterial activity and the minimal inhibitory concentration against pulmonary disease-causing Klebsiella pneumoniae, Streptococcus pyogenes, and Streptococcus pneumoniae. The results obtained were as follows: 1. Among the 23 oriental medicines tested, the water-soluble extracts of Coptis japonica, Scutellaria baicalensis and Picrorrhiza kurrooa showed the antibacterial activity against K.pneumoniae and that of C. japonica against S. pyogenes. The antibacterial activities of C. japonica, Prunusmume, Schizandra chinesis, Scutellaria baicalensis were also found against S.pneumoniae. When C.japonica was used, the high antibacterial activity was shown against Bacillus subtilis and other extracts showed a little activity against B. subtilis and E. coli as a control. 2. The ethanol-soluble extracts of Patrinia scabriosaefolia, P. mume, S. baicalensis, S. chinesis showed the antibacterial activity against K. pneumoniae and those of S. baicalensis, C. japonica, S. chinesis, P. mume agaist S. pyogenes and S. pnuemoniae. However, those extract showed a little antibacterial activity against B. subtilis and E. coli except for that the extract of C. japonica showed comparatively high growth inhibition of B. subtilis. 3. Among the medicinal herbs tested, the water and ethanol extrats of C. japonica showed very extcellent antibacterial activity against the pathogenic bacteria and controls. 4. When the water-soluble extracts of C. japonica and S. baicalensis, minimal inhibitory concentrations (MICs) against K. pneumoniae were $10mg/m{\ell}$ and $22 mg/m{\ell}$, respectively. The MICs of the ethanol-soluble extracts of P. mume and P. scabriosaefolia were $5mg/m{\ell}$ and $20mg/m{\ell}$, respectively. 5. For the MICs against S.pyogenes, C. japonica showed $15mg/m{\ell}$ with the water-soluble extract and P. mume and C. japonica with the ethanol-souble extract did $5mg/m{\ell}$ and $10mg/m{\ell}$, respectively. 6. For the MICs against S. pneumoniae, C. japonica and P. mume with the water- and ethanol-souble extract showed $5mg/m{\ell}$ and $10mg/m{\ell}$, respectively. As a result, the highest antibacterial activity was found in the water- and ethanol-soluble extracts of C. japonica against pulmonary disease-causing bacteria, K. pneumoniae, S. pyogenes. and S. pnuemoniae. Also, the water- and ethanol-soluble extracts of S.chinesis. P.mume, S.baicalensis, and P.kurrooa showed hight antibacterial activities.