• Title/Summary/Keyword: 세균성

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Diversity of Pigment-Producing Halophilic Bacteria Isolated from Coastal Seawater and Solar Saltern in Korea (한국 근해와 염전에서 분리한 색소 생성 호염성 세균의 다양성)

  • Yong Hae-Young;Park Jin-Sook
    • Korean Journal of Microbiology
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    • v.40 no.4
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    • pp.302-306
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    • 2004
  • A total of forty strains of pigment-producing halophilic bacteria were isolated from the solar saltern and coastal seawater in Korea. The diversity of those bacteria were determined on the basis of PCR-RFLP and 16S rDNA sequences. The isolated strains were clssified into nine genera: Pseudoalteromonas, Photobacterium, Vibrio, Halobacillus, Bacillus, Paracoccus, Salinicoccus, Tenacilbaculum, and Flavobacterium. While more than $80\%$ of the pigment-producing halophilic bacteria isolated from the coastal seawater were classified as gram-negative Pseudolateromonas, most of the strains isolated from the solar saltern were classified into gram-positive Halo­bacillus. The other strain was KK7, which may be identified as novel species belonging to the genus, Salini­coccus.

Numerical Analysis of Heterotrophic Bacterial Community in the Sudong Stream (수동천에서의 종속영양세균 군집에 대한 수리학적 분석)

  • 최성찬;김상종
    • Korean Journal of Microbiology
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    • v.25 no.4
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    • pp.318-327
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    • 1987
  • Taxonomic composition and diversity were wxamined by statistical analysis for bacterial communities in surface waters of the Sudong Stream, a tributary of North Han River. For total 282 isolates, Flavobacterium, Aeromonas and Enterobacteriaceae was identified by the deterministic schemes as a major group above 50% of total isolates in all sampling sites. Morphological, biochemical and physiological characteristics were numerically analyzed for bacterial isolates from each site and clustered into 15-28 groups. Not all statistically clustered groups were identical to the groups derived from deterministic identification. Especially, consistent relationship was not found in dendrograms for the groups with each a single strain which gas peculiar sugar-degrading activity. At a level of 80% similarity, bacterial diversity (H) was ranged as 2.37-3.14, and it was suggested that the research area was oligotrophic-mesotrophic status. Regional distribution of bacterial community was most heterogeneous at the site where large input of allochthonous materials or bacteria were occurred. And that was the significant factor for the compositions of bacterial communities in the Sudong stream.

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Fecal Microflora of Mice in Relation to Diet (식이에 따른 장내세균의 효소활성 및 장내세균층의 비교)

  • 최성숙;하남주
    • Korean Journal of Microbiology
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    • v.35 no.2
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    • pp.128-132
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    • 1999
  • The effects of diet on the composition of fecal microflora in mouse and the aclivilies of several enzymes in the leces were investigated. Vegetarian dietary groups were found to contain about ten lines higher numbers of Locmbocillus and B$&bacterizml than animal dietary groups. An~rnal dietmy groups were found to contain about 5 tolo times higher numbers of anaerobic Closhidia and Bocieriocles than the vegeterian detary groups. Fccal microbial $\beta$-glucosidase, $\beta$-glucm'onidase, ii-yptophanase and orease activilies in ihe animal dietary groups were shown lo be 30 to 50% hgher than those in h e vegetarian detary groups.

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Immune Response of Bacterial Proteins of Staphylococcus intermedius from Canine Atopic Dermatitis (개의 아토피성 피부염에서 분리한 Staphylococcus intermedius 균의 세균단백질의 면역반응)

  • Park, Hee-myung
    • Journal of Veterinary Clinics
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    • v.21 no.1
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    • pp.20-22
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    • 2004
  • Bacterial infection of canine atopic dermatitis is largely caused by Staphylococcus intermedius and may be a superficial or deep pyoderma. The Purpose of this study was to identify the major proteins of S. intermedius cell surface components in humoral immune response of atopic dermatitis dog. Sera samples were obtained from dogs with atopic dermatitis and superficial pyoderma referred to the Veterinary Medical Teaching Hospital, Konkuk University. An isolate of S. intermedius from a clinical case of canine atopic dermatitis was cultured in brain heart infusion broth overnight at $37^{\circ}C$ in aerobic conditions on an orbital shaker. Following culture, Staphylococci were harvested by centrifugation, washed in PBS, and resuspended in PBS containing lysostaphin. The soluble components were separated by centrifugation and were collected. The soluble extract of S. intermedius was separated by sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE). The proteins were electrophoretically transferred onto nitrocellulose membrane. Western blotting for the specificity of serum IgG antistaphylococcal antibody was performed with anti-dog-IgG and sera obtained from an atopic dermatitis case and a normal dog. The molecular masses of four major proteins of S. intermedius recognized by serum obtained from an atopic dermatitis case were 18, 31, 75, and 110 kDa as determined by Western blot analysis. The present study indicates that most dogs of S. intermedius infection with atopic dermatitis could have a significant humoral immune response to bacterial proteins of the causative organism.

C-reactive Protein Level in a Variety of Infectious Diseases (감염성 질환들에서 C-반응 단백 값)

  • Kim, Hyo-Jin;You, Jung-Hoon;Lee, Kyung-Yil
    • Pediatric Infection and Vaccine
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    • v.12 no.2
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    • pp.101-107
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    • 2005
  • Purpose : We evaluated the C-reactive protein(CRP), white blood cell(WBC) and neutrophil levels in the various infectious diseases in a single hospital. Methods : A total of 640 medical records of children with infectious diseases such as bacterial meningitis(19 cases), acute pyelonephritis(55 cases), measles(253 cases), chicken pox (38 cases), mycoplasma pneumonia(160 cases), tsutsugamushi disease(39 cases) and Kawasaki disease(152 cases) admitted to The Catholic University of Korea, Daejeon St. Mary's hospital from 1996 to 2002 were retrospectively analyzed. Results : The mean CRP level was $17.9{\pm}6.4mg/dL$ in bacterial meningitis, $9.1{\pm}5.6mg/dL$ in Kawasaki disease, and $8.1{\pm}3.3mg/dL$ in acute pyelonephritis. In the mycoplasma pneumonia and tsutsugamush disease group(atypical bacterial group), the CRP level was $3.2{\pm}2.5mg/dL$, and $1.0{\pm}0.8mg/dL$ in the viral diseases group(measles and chicken pox). There were also significant differences for the WBC count and neutrophil differential between the 3 infectious groups with higher level in the bacterial infections group($15,600{\pm}6,100/mm^3$, $62{\pm}21%$) than in the atypical bacterial infections and in the viral infections group($9,600{\pm}3,300mm^3$, $57{\pm}11%$ and $7,300{\pm}2,900/mm^3$, $49{\pm}16%$, respectively). The inflammatory indices in Kawasaki disease were like those of bacterial infections. There was a correlation between CRP level and WBC or neutrophil count in the bacterial infections and Kawasaki disease groups. Conclusion : The CRP, WBC and neutrophil levels showed a clear difference between the infectious diseases according to causative agents. The WBC and neutrophil level was different according to age in measles and mycoplasma pneumonia. There was a correlation between CRP level and WBC or neutrophil count in the bacterial infections and Kawasaki disease groups.

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Causes of Diarrhea in Summer (여름철 설사의 원인분류)

  • Park Sung Ham
    • The Korean Nurse
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    • s.6
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    • pp.14-21
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    • 1963
  • 여름철에 유생하는 설사의 원인의 대부분은 세균성인 것이며 이 세균성으로 인한 설사는 그 세균자체가 원인이 되는 것과 또 그 세균이 생산한 독소가 원인이 되는 것이 있다. 특히 여름에는 자연조건 즉 높은 기온과 습도가 미생물의 발육에 좋은 환경을 제공하며 겸하여 파리 등 곤충매개체가 이를 운반하는 관계상 오염된 음식물을 섭취하여 생기는 급성위장염 즉 소위 식중독이 빈번하게 일어나는 것이다. 작년만 하더라도 서울시내 일류 화식요정에서 만든 ''도시락''에 의하여 집단식중독이 생긴 사실들이 아직 우리들의 기억에 새롭거니와 세균 이외에도 이질아메바 등, 기타 원충감염으로 생기는 설사, Virus로 생기는 설사, 기타 식중독 또는 화학약제 등에 의하여 생기는 설사 등이 있는데 이를 크게 구분하여 보면 다음과 같다.

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Shot Hole of Peach and Japanese Plum Caused by Xanthomonas campestris pv. Pruni and Erwinia nigriflens in Korea (Xanthomonascampestris pv. pruni와 Erwinia nigrifluens에 의한 복숭아 및 자두의 세균성구멍병)

  • 최재을;이은정;박영섭
    • Research in Plant Disease
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    • v.6 no.1
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    • pp.10-14
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    • 2000
  • In 1998, bacterial shot hole of peach (Prunus persica) and Japanese plum(Prunus salicina) was found in Naju and Milyang. Five isolates of bacteria isolates from the diseased leaves and fruits of peach and Japanese plum were classified into genus Erwinia and Xanthomonas on diagnostic characteristics. Of five isolates, two were identified as X. campestris pv. pruni, three as E. nigrifluens. E.nigrifluens is the first description of bacteria which causes the disease on peach and Japanese plum in Korea. the symptoms caused by E. nigrifluens were hardly distinguished from those caused by X. campestris pv. pruni. In addition, it was observed that two pathogenic bacteria were isolated from most of naturally infected plants at the same time. from the reason mentioned above, we proposed to use a single common name \"bacterial shot hole of peach and Japanese plum\" for the both bacterial diseases, hereafter.

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광합성세균을 이용한 수소생산

  • 김진상
    • Journal of Life Science
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    • v.2 no.3
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    • pp.175-179
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    • 1992
  • 광조사시에 수소를 생산하는 미생물로는 녹조류, 남조류 그리고 광합성세균이 알려져 있으며, 이 중에서 남조류와 광합성세균이 실용적인 수소생산에 유망시되고 있다. 광합성세균은 광학계 II가 결여되어 물분해능이 없으나 유기화합물을 전자공여체로하여 남조보다 훨씬 빠른 속도로 수소를 생산하며, 생산가스는 약간의 이산화탄소 외에는 거의 순수한 수소여서 그대로 연료로 사용할 수 있는 장점을 지닌다. 본고에서는 공합성세균에 의한 수소생생의 연구현황과 문제점에 대해 다루었다. 광합성세균에 의한 수소생산의 실용화를 위해서는 균체의 수소생산성 향상 및 활성의 유지, 원료문제 및 암모니아에 의한 수소 생산의 억제문제, 적합한 배양조개발과 균체의 이용방안 등에 관련된 제분제의 해결이 필요하다. 광합성세균의 수소생산성 향상을 위해서는 자연계로부터 보다 고활성균주의 탐색과 아울러 유전적인 개량이 병행되어야한다.

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