• Korean journal of applied entomology
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• v.61 no.1
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• pp.1-14
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• 2022

Moths have a well-developed sex pheromone communication system. Male moths exhibit an extremely sensitive and selective sex pheromone detection system so that they can detect the sex pheromone produced by conspecific females and locate them for successful mating. Using the pheromone detection system, male moths display characteristic stereotypic behavioral responses, flying upwind to follow intermittent filamentous pheromone strands in pheromone plume. The chemical composition of female sex pheromone in moths, typically comprised of multiple compounds, is species-specific. Male moths contain specialized pheromone receptor neurons on the antennae to detect conspecific sex pheromone accurately, and distinguish it from the pheromones produced by other species. The signals from pheromone receptor neurons are integrated and induce relevant behavior from the male moths. Male moths also contain olfactory sensory neurons in pheromone sensilla, specialized for pheromone-related behavioral antagonist compounds, which can enhance discrimination between conspecific and heterospecific pheromones. Here we review reports on the sex pheromone detection system in male moths and their related responses, and suggest future research direction.

• Korean journal of applied entomology
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• v.61 no.1
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• pp.35-50
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• 2022

In this review, the compositions of the sex pheromones of 22 lepidopteran species were analyzed and briefly arranged by comparing the research results conducted in different locations worldwide. These compositions can be used as monitoring tools for pests of rice, maize, leguminous crops, perilla, and sesame in Korea. The sex pheromone compounds and those compositions of four species were successfully identified within Korean populations. The optimal compositions of sex pheromones of three other species were selected through behavioral studies using synthetic chemicals of the known pheromone compounds in laboratories and fields, without chemical identification processes in the sex pheromone compounds of Korean populations. For a few species, the roles of the minor components of the sex pheromone volatiles should be re-assessed for use in the development of optimal monitoring lures.

• Korean journal of applied entomology
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• v.60 no.4
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• pp.363-367
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• 2021

This study was conducted to investigate the EAG (electroantennogram) response of Spodoptera frugiperda male to sex pheromone compounds and whether or not S. frugiperda male adults would undergo double mating. The EAG response of S. frugiperda male adult to Z9-14:Ac increased in a dose-dependent as the dose increased. Among the 7 sex pheromone components investigated, male EAG recording was the highest to Z9-14:Ac. The EAG response of S. frugiperda male adult to the mixed sex pheromone component was greater than that to the single component. Male adults of S. frugiperda were capable of double mating under laboratory condition, and the secondary mating rate increased to 72.2% compared to the 58.3% of primary mating rate. The EAG response of mated S. frugiperda male adult was not different from that of unmated S. frugiperda male. In the net house test with sex pheromone lure, mated male adults were not captured during the test period. Also, strangely, unmated male adults were not captured even in a trap equipped with virgin female adults, although the antennae of mated male adult were responded to the sex pheromone component in the laboratory. Probably, it is thought that the mated male adults may not have been caught in the trap be due to flight ability which has been decreased after mating. The field attractiveness of S. frugiperda male adults to sex pheromones remains to be further elucidated.

• Korean journal of applied entomology
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• v.53 no.3
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• pp.239-246
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• 2014

Two congener species of Grapholita molesta and G. dimorpha have similar host ranges and adult occurrence patterns. In addition, the two species commonly use cis-8-dodecenyl acetate (Z8-12:Ac) and trans-8-dodecenyl acetate (E8-12:Ac) as their major sex pheromone components. The commercial lures made of the two components, therefore, suffer a mixed attraction of these two species. This study was conducted to assess any effect of their known minor sex pheromone components to reduce the mixed attraction. When cis-8-tetradecenyl acetate (Z8-14:Ac) was added to the commercial sex pheromone consisting of Z8-12:Ac/E8-12:Ac = 85/15, the attraction to G. dimorpha was not affected, but its attraction to G. molesta was significantly reduced. However, the addition of either dodecanyl acetate (12:Ac) or tetradedecanyl acetate (14:Ac) did not influence on the attraction of both species to the commercial lure. The addition of E8-14:Ac to 3-component sex pheromone, Z8-12:Ac/E8-12:Ac/Z8-14:Ac = 85/15/10, the attraction to G. dimorpha was rather significantly suppressed. From these results, the 3-component sex pheromone, Z8-12:Ac/E8-12:Ac/Z8-14:Ac = 85/15/10 was suggested as an improved composition of sex pheromone lure for G. dimorpha.

• Korean journal of applied entomology
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• v.46 no.3
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• pp.349-356
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• 2007

Three active components (Z8-12:Ac, E8-12:Ac, and Z8-12:OH) are known in sex pheromone of the oriental fruit moth, Grapholita molesta, and have been commercially available to apply for population monitoring and mating disruption. However, there have been variation among commercial products in pheromone composition and amount impregnated in each pheromone releaser. This study was performed to optimize factors influencing on pheromone monitoring of G. molesta by analyzing pheromone composition/amount, effective period of releaser, and trap type/placement in apple trees. High purity of Z8-12:Ac component was effective to attract males, in which 96% or more of cis acetate isomer component appeared to be optimal composition. Pheromone amounts ($0.01-1\;{\mu}g$) impregnated in each rubber dispenser did not give significant effect on monitoring during 90 days from June to August. "Delta" trap was much more efficient than "cone" trap to collect males. Trap installation was another factor, in which placing traps at canopy level was much efficient than at trunk or ground levels. Pheromone trap monitoring with these optimal factors indicated three to four adult peaks from June to September in addition to high overwintering population from April to May in pesticide-applied orchards in Andong, Korea.

• The Korean Journal of Pesticide Science
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• v.17 no.3
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• pp.200-205
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• 2013

Mating disruption experiments were evaluated and compared in several pheromone traps and sex pheromone composition ratios at commercial chestnut garden for developing alternative control method of D. punctiferalis. The highest attractive was shown in Delta traps at the effective sex pheromone ratio of 75:25 (E10-16:A1:Z10-16:A1). Comparisons of domestic and imported pheromone constituents were not significant difference in 90:10 (E10-16:A1:Z10-16:A1). But, there was significant difference in sex pheromone ratio of 75:25. D. punctiferalis was caught the highest number in Gongju area for attractive test using pheromone trap. Attractive effects with dose of pheromone were showed the highest at 2.5 mg/lure and 1 mg/lure in Gongju area and Cheongyang area respectively. However, there was no significant difference among pheromone dose in Buyeo area. These data could be useful for eco-friendly managing of D. punctiferalis in the chestnut farm.

• Korean journal of applied entomology
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• v.44 no.1 s.138
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• pp.31-36
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• 2005

Adoxophyes spp. are the major rests of a pear. The larvae attack both leaves and fruits. (Z)-9-tetradecenyl acetate (Z9-14:Ac), (Z)-11-tetradecenyl acetate (Z11-14:Ac), (E)-11-tetradecenyl acetate (E11-14:Ac) and 10-methyldodecyl acetate (10me-12:Ac) have been reported as the sex pheromone components of the genus Adoxophyes. Our objective was to determine the difference in sex pheromone composition of three different A. spp. populations each from Cheonan, Sangju, and Naju area orchards in Korea. Gas chromatography (GC) analyses of pheromone gland extracts of virgin females confirmed the presence of two compounds (Z9-14:Ac and Z11-14:Ac) in Cheonan and Sangju populations, and four compounds (Z9-14:Ac, Z11-14:Ac, E11-14:Ac and tome-12:Ac) in Naju population. The Z9-14:Ac and Z11-14:Ac were detected in the ratio of 80:20 in the Cheonan population and 3:97 in the Sangju population. Females of Naju population produced sex pheromone blend consisting of Z9-14:Ac, Z11-14:Ac, E11-14:Ac and 10me-12:Ac at a ratio of 31:62:6:1. Field trapping tests in pear orchards with Z9-14:Ac and Z11-14:Ac indicated that maximum captures of the male were obtained with traps baited by 80:20 in Cheonan, 10:90 in Sangju, and 30:70 in Naju. These results suggest that there are remarkable geographical variations in the sex pheromone composition of A. spp. in pear orchards in Korea, and taxonomic classification of these species must be carefully assessed.

• Korean journal of applied entomology
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• v.40 no.3
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• pp.219-226
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• 2001

To identity the composition and ratio of the sex pheromone in the asiatic leafroller, Archippus breviplicanus (Walsingham), which is a major pest on apple leaves, and to develop a monitoring system using this sex pheromone, abdominal tips of their virgin female during the calling period were extracted and analysed. The GC/GC-MS analysis revealed the following three compounds; (E)11-tetradecenyl acetate (E11-14: Ac), (Z)11-tetradecenyl acetate (Z11-14: Ac), and tetradecyl acetate(14: Ac) with the ratio of 56:22:22. Electroantennogram (EAG) assay was also conducted to examine biological activity of the components. The three components evoked significantly higher responses than hexane or air but there was no significant differences between the mixtures made by components. Field trapping experiments for determining the optimum blend, seasonal fluctuation and the optimum amount for male attracting were carried out over 4 years from 1997 to 2000. Although male moths were attracted to 8:2 and 7:3 between E11-14: Ac and Z11-14: Ac, their 7:3 blend was found to be the most effective. Archippus breviplicanus appears to occur three times a year in Korea. The optimum amount for monitoring was found to be 1mg per trap. 14: Ac, the third component, did not show synergistic effect on male moth attraction.

• Korean journal of applied entomology
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• v.39 no.2
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• pp.105-110
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• 2000

Sex pheromone composition was analyzed for Dichocrocis punctiferalis, which attacksapple, peach, chestnut fruits, etc., and its behavioral and field trapping studies were conducted to developa monitoring system with its sex pheromone. Virgin females showed maximum mating behavior andhairpencil extrusion behavior between 4-5 hrs after lights-off under 16L18D photoperiod and 26rf 1 "C.During this period, two sex pheromone components, ElO-hexadecenal and ZlO-hexadecenal, weredetected by GC analysis in the hexane extract of abdominal tips of virgin females. The best attraction ofmales to various synthetic sex pheromone blends was obtained at the 70 : 30 ratio of ElO-hexadecenal andZ10-hexadecenal for hair pencil extrusion and at the 80 : 20 ratio for flying upwind response. The highestattractiveness in fields was obtained between 70 : 30 and 80 : 20 from several tests in apple, peach andchestnut orchards.and chestnut orchards.

• Korean journal of applied entomology
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• v.36 no.4
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• pp.323-330
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• 1997

The apple leafminer, Phyllonorycrer ringoniella (Lepidoptera: Gracillariidae), is the most important pest on apple leaves and is not easy to be controlled with insecticides because of leafmining habit of its larvae. This study was undertaken to develop an artificial diet for mass rearing of the moth, and to look at their reproductive behavior. For developing an artificial diet the problem of diet contamination with microorganisms and how to supply artificial diet to first instar larvae have to be solved first. Artificial oviposition trial was successful with shadowy bottomside of various papers sprayed with apple leaf extracts. The highest activity of both calling and mating was observed within 30 min. after the onset of photophase and 3- to 4-day -old adults showed the highest mating rate. A ring-shaped sex pheromone gland was found at the intersegmental membrane between the 8th and 9th abdominal segments of the female. (E, 2)-4,lOtetradecadienyl acetate was identified as the major component from the femall gland extracts by the gas chromatography (GC) and GC-mass spectrometry. Additionally it was thought that (a-10-tetradecenyl acetate could be the second component of their sex pheromone.