• Title/Summary/Keyword: 성장과 발달

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Somatic Embryogenesis and Plant Regeneration in Embryogenic Cell Suspension Cultures of Hovenia dulcis Thunb (헛개나무의 현탁배양세포로부터 체세포배발생과 식물체 재생)

  • Li, Cheng-Hao;Zhao, Bo;Kim, Na-Young;Kim, Myong-Jo;Cho, Dong-Ha;Lee, Dong-Wook;Lee, Jae-Geun;Lim, Jung-Dae;Yu, Chang-Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.14 no.4
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    • pp.255-260
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    • 2006
  • Culture conditions for high frequency plant regeneration via somatic embryogenesis from embryogenic cell suspension cultures of Hovenia dulcis are described. Germinated somatic embryos were selected for induction of secondary embryogenesis. Friable embryogenic cells were induced directly from somatic embryos when transfer to 1/3 MS solid or liquid medium lacking plant growth regulators. The temperature strongly effected on induction of secondary embryognesis than other conditions in culture. All somatic embryos produced friable embryogenic cell clumps within 10 days when germinated somatic embryos cultured in 1/3 MS medium at $30^{\circ}C$ in suspension culture. No somatic embryos formed from embryogenic cell suspension cultures at $18^{\circ}C$. Numerous somatic embryos were induced and subsequently developed uniformly into germination stage from suspended cell clumps after 4 weeks of culture on $18^{\circ}C$. Plantlets conversion were observed on $18^{\circ}C$ when germinated somatic embryos were transferred to 1/3 MS solid medium without plant growth regulators or supplemented with 0.1-0.5 mg/l benzyladenine.

Design and Implementation of a Distribute Multimedia System (분산 멀티미디어 스트리밍 시스템 설계 및 구현)

  • Kim, Sang-Kuk;Shin, Hwa-Jong;Kim, Se-Young;Shin, Dong-Kyoo;Shin, Dong-Il
    • Annual Conference of KIPS
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    • 2000.10a
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    • pp.677-680
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    • 2000
  • 웹이 등장하면서 지금까지 인터넷 상에서 텍스트와 이미지를 이용하여 정보를 표현하고 전달하는 방법이 가장 많이 사용되어왔다. 그러나 웹 관련 기술의 비약적인 발달과 네트워크 속도의 증가 및 인터넷의 급속한 보급으로 단순한 텍스트와 이미지 중심의 HTML 문서를 이용한 정보의 전달이 아닌 멀티미디어 데이터를 이용한 정보의 표현과 전달이 점차 증대되고 있다. 이에 따라 멀티미디어 데이터를 전송하기 위한 스트리밍 프로토콜도 등장하였다. 최근에는 컴퓨터의 성능 증가 및 네트워크 속도의 증가(초고속 통신 서비스의 보급)에 의해 멀티미디어 데이터의 전송이 가능하게됨으로써 기존의 공중파나 CATV 방송국의 형태 지니고 인터넷 상에서 실시간 생방송 서비스와 VOD(Video On Demand) 서비스를 제공하는 인터넷 방송국이 급속하게 생겨나고 있다.[11] 인터넷 방송은 동영상과 오디오의 실시간 전달을 가능하게 하는 멀티미디어 스트리밍 기술과 멀티미디어를 실시간으로 전송할 수 있는 실시간 전송 프로토콜을 기반으로 발전하고 있다. 인터넷 상에서 멀티미디어 스트리밍 서비스를 하는 대부분의 인터넷 방송은 스트리밍 서버로서 RealNetworks사의 RealSystem과 Microsoft사의 WMT(Windows Media Technologies)를 사용하고 있다. 본 논문은 Real Server와 WMT의 비교 분석을 통해 실시간 전송 프로토콜을 지원하고, 멀티미디어 스트리밍 기술을 지원하는 자바를 기반으로 한 분산 서버 구조의 스트리밍 서버, 서버간의 부하를 제어하는 미들웨어, 멀티미디어 스트림을 재생할 수 있는 클라이언트를 설계하고 구현한다. 방법에 대해서 자세히 살펴보고 실제 SQL Server 7.0 환경에서 구축된 공간 엔진 및 OLE DB 제공자 컴포넌트의 구현 예에 대하여 살펴볼 것이다. 혈액내방사선 조사량이 안전용량 범위(200rad)에 속하며 48시간 후 체내잔류량은 서양인과 큰 차이가 없었다.비출현의 소견을 보이는 악성종양 환자의 골 신티그람 53개중 44개 (83.0%)에서 척추 및 늑골에 미만성, 또는 다발성 침습이 관찰되었다. 또 골전이 부위를 두개골, 척추, 견대부, 늑골, 골반, 사지의 근위부 장골의 6개 부위로 나누어 분석할 경우 49개(92.5%)에서 3부위 이상에 전이가 발견되었고, 35개(66.0%)에서 4부위 이상에 전이가 발견되었으며, 5부위 이상, 6개 부위에 모두 전이가 발견된 것은 각각 20개 (37 7%), 11개(20.8%)이었다. 이상의 성적으로 보아 악성종양 환자의 골 신피그라피에서 신장 영상의 비출현은 종양의 광범위한 골전이를 간접적으로 시사하는 소견으로 생각된다. 여러 악성종양중 전립선암에서 신장 영상 비출현의 빈도가 가장 높았으며, 특히 위암에서 골전이 및 신장 영상 비출현의 빈도가 높음은 주목할 만한 것이라 하겠다.출한 결과 인,규소 증가와 자가영양성 미소플랑크톤(ANP)증가에 미치는 요인이 해안과 외해에서 동일하게 가장 큰 설명력을 보였다. N:P 비도 해안에서 36.4, 외해에서 32.6을 보이고 있어 인이 상당히 부족한 것으로 나타났다. 따라서 조사해역은 인이 식물플랑크톤 성장에 중요한 제한요인으로 작용하고 있다고 판단된다.의 회전. 전위력의 강도, 적용시점, 그리고 키, 체중등의 신체적 요인 등이 있으나 능숙한 기계사

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Study on Relationship between the Obesity Index and the Refractive Power in Adolescent (청소년기 비만도 지수와 굴절력과의 상관성 연구)

  • Lee, Wan-Seok;Ye, Ki-Hun;Shin, Bum-Joo
    • Journal of Korean Ophthalmic Optics Society
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    • v.18 no.3
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    • pp.329-339
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    • 2013
  • Purpose: In this study, nutrient intake during the adolescent period is a critical time for the development of the vision. Therefore we analyzed the relationship between the obesity index and the refractive power. Methods: We used the Korean National Health and Nutrition Examination Survey 2010 document. The obesity index is classified as Broca index (less than 80.0% is low weight, 80 to 89.9% is underweight, 90.0 to 109.9% is normal, overweight is from 110.0 to 119.9%, 120.0% or more is obesity) and body mass index (BMI)(less than 18.5 is underweight, 18.6~22.9 is normal, 23.0 to 24.9 is overweight, and obesity is higher than 25.0). We analyzed correlation with the body mass index and refractive error in adolescent. Results: As shown in the statistics, according to Broca index and body mass index (BMI), the refractive power and the obesity index showed a statistically significant correlation in the ages 15 to 18. Under weight subjects are ingested 43.84% dietary fiber, vitamin C 56.55%, 35.20% iron, 31.84% of, and 38.03% potassium less than the average food intake by the standard group. Conclusions: Moderate weight with a broad variety of taking nutrition and good eating habits seems to have an effect to the good growth and the good vision.

Eggs Development and Larval Development of the Ice Fish, Hypomesus transpacificus nipponensis McAllister (빙어의 난발생과정과 자어의 형태발달)

  • HAN Kyeong-Ho;LEE Seung-Ju;KIM Yong Uk;MYOUNG Jung-Goo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.4
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    • pp.497-502
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    • 1996
  • Artificial fertilization of ice fish, Mypomesus transpaciticus nipponensis caught at Milyang-river and Osib-chun brook was performed in March 24, 1990, and the hatched larvae were reared for 25 days to describe the development of eggs and larvae. Fertilized eggs were spherical in shape, measuring $0.85\~1.05\;mm$ in diameter (mean: 0.97 mm) and translucent adhesive with many small-sized oil globules on the surface. Hatching in the indoor tank started from the 170 hours after fertilization under $16.5^{\circ}C$ water temperature. Newly-hatched larvae were measured $3.85\~4.25\;mm$ in total length (mean: 4.05 mm), and mouth and anus were not yet open. They had one yolk sac on the anterior part of abdomen, straight-type's notochord, and $52\~54$ myotomes. The larva of 5 days old transformed to postlarval stage and measured $5.20\~5.65\;mm$ (mean: 5.37 mm) in total length. As the yolk sac was completely absorbed, mouth and anus were open, and they fed rotifers vigorously. In 20 days after hatching, the larvae grew to 8.38 mm in TL, and the caudal notochord flex at $45^{\circ}$. In 25 days after hatching, total length reached 9.63 mm. The pan of the fin-fold of the future dorsal and anal fins became high.

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Annual Reproductive Cycle of the Banded Catfish, Pseudobagrus fulvidraco (Richardson) (동자개, Pseudobagrus fulvidraco (Richardson)의 생식년주기)

  • LIM Sang-Koo;HAN Chang-Hee
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.5
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    • pp.823-833
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    • 1997
  • To clarify the annual reproductive cycle of the banded catfish, Pseudobagrus fulvidraco (Richardson), the seasonal changes in histological aspect of gonad and liver were examined. The adult fish was raptured from the upper stream of Young-San river, Chunnam in each month from May 1992 to June 1993. Based on the annual changes in GSI (gonadosomatic index), HSI (hepatosomatic index), CF (condition factor) and histological aspects of the gonads, the annual reproductive cycle were classified into 5 periods as follows: 1) Growing phase (from April to early May): The value of GSI increased and the size of oocytes in perinucleolus stage in oocytes increased gradually. Spermatogonia were developed actively from the epithelial tissues of seminiferous tubules. 2) Maturing phase (from Hay to early June): GSI levels increased rapidly in both sex. Oocytes at various developmental stages were observed. Appearance of active spermatogenesis were observed. 3) Mature and spawning phase (from June to August): High values of GSI remained static and oocytes accumulated significant quantitis of yolk globules. 4) Degenerating phase (from September to November): GSI levels decreased and ovaries were filled mostly with oocytes at the perinucleolus stage. Hepatic cells accumulated significant amounts of lipid droplets. 5) Resting period (from December to March) : Low values of GSI were kept and the size of oocytes at the perinucleolus stage did not increase. Spermatogenesis was not observed.

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A Study on Teaching Artists competencies as educator for animation arts and culture education (애니메이션 문화예술교육을 위한 에듀케이터로서의 문화예술교육사 역량 연구)

  • Kim, Jae-Woong
    • Cartoon and Animation Studies
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    • s.45
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    • pp.235-258
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    • 2016
  • As the first class Teaching Artists system is about to be brought to effect, the ability required for Teaching Artists to design educational contents, develop and manage education programs is not much different from that of a museum Educator. This system is necessary for resolving problems in existing Arts and Culture Education, such as overlapping programs and adjusting difficulty levels by age to meet the demand of educatee. It also deals with drawbacks in production-oriented curriculum originating from the preference in some subject. In addition, as progress in science and technology makes rapid changes in digital media and its subculture, increasing need for novel and interdisciplinary curriculum in the field of Arts and Culture Education puts further emphasis on the importance of this system. In this study, we focus on clarifying the significance of Educator as a professional and proposing curriculum for the system, trying to avoid restricting our discussion to current Arts Instructor Supporting Project which are merely aimed at supporting children and adolescents to grow up to enjoy culture and arts. Capacity for designing curriculum for culture and arts, the kernel of qualification for the first class Teaching Artists, requires a variety of comprehensive expertise and qualification such as doing preliminary research on contents related to animation, curating, determining potential of educational contents, organizing educational contents for appropriate educatee, understanding esthetic property and its role in education, and appreciating and enjoying cultural contents. Therefore, Teaching Artists plays roles not only in developing and running educational programs but also in supporting and cooperating with culture and arts institutions, designing and managing creative programs, combining and communicating with different social groups, and emphasizing mutual interchange in culture.

Discordant Congenital Cytomegalovirus Infection in Twins (쌍생아에서 선천 거대세포바이러스 감염 증례)

  • Kim, Yi-Seul;Kang, Ji-Man;Lee, Ji-Hoon;Chang, Yoon Sil;Park, Won Soon;Kim, Yae-Jean
    • Pediatric Infection and Vaccine
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    • v.24 no.1
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    • pp.65-70
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    • 2017
  • Cytomegalovirus (CMV) infection is one of the most common congenital infections. The first case of discordant congenital CMV infection in twins occurred in Korea. A 31-year-old woman became pregnant with twins (dichorionic-diamniotic). An elective caesarean section was performed at 37 weeks. The first baby was male, weighing 2,410 g with an Apgar score of 8/9. The second baby was female, weighing 1,380 g with an Apgar score of 5/8. She had experienced intrauterine growth retardation, and presented with microcephaly, micrognathia, and joint stiffness. During the work-up for discordant twins, the second baby's serum test was positive for CMV immunoglobulin M. Her urine, blood, and cerebrospinal fluid (CSF) were CMV polymerase chain reaction positive. The first baby's CMV tests were negative. Ophthalmologic exam and audiometry performed on the second baby showed CMV retinitis and bilateral sensorineural hearing loss. She was treated with intravenous ganciclovir. Currently, she is bed-ridden and has significant developmental delay. Although the causes of discordant congenital CMV infection in twins are unclear, this case shows that discordant congenital CMV infection should be considered in twins with significant differences in intrauterine growth or clinical symptoms after birth.

A Twin diagnosed with Duarte Variant/Classical (D/G) Galactosemia (Duarte Variant/Classical Galactosemia (D/G) Heterozygote으로 진단된 일란성 쌍둥이 1례)

  • Koo, Kyo Yeon;Lee, Chul Ho;Yang, Jeong Yoon;Lee, Jin-Sung
    • Journal of The Korean Society of Inherited Metabolic disease
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    • v.12 no.1
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    • pp.58-63
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    • 2012
  • Classical galactosemia (OMIM# 230400) is an autosomal recessive disorder of carbohydrate metabolism, due to a complete loss in galactose-1-phosphate uridyltransferase (GALT; E.C.2.7.7.12) enzyme activity. It caused by mutations in the GALT gene (OMIM$^*$ 606999) that is located at chromosome 9p13. The GALT enzyme deficiency results in a build-up of galactose and galactose-1-phosphate, causing life threatening complications such as feeding problems, failure to thrive, hepatocellular damage, bleeding and sepsis. However, Duarte galactosemia, a variant form of GALT deficiency, has residual GALT enzyme activities in erythrocytes and do not have manifest the symptoms of classical galactosemia. Since the advent of newborn screening (NBS) for galactosemia, we rarely encounter such overwhelmingly ill newborns. The positive NBS with no symptoms indicates the possibility of Duarte galactosemia besides a simple false positive and it has to be differentiated from classical galactosemia which is a medical emergency. In Korea, detection rate of Duarte galactosemia is very low and its genetic information is restrictive, too. We report a case of monozygotic twins with D/G galactosemia compound heterozygote in proven by the mutational analysis of GALT gene, which revealed N314D polymorphism and -119 to -116 delGTCA.

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Evaluating the Trapping Efficiency of Vegetative Buffer Systems on Sediment Reduction Using SWAT model (SWAT 모델을 이용한 수변 완충지역에서 비점오염원 저감효율 평가)

  • Kim, Ik-Jae;Son, Kyong-Ho;Kim, Jeong-Kon
    • Proceedings of the Korea Water Resources Association Conference
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    • 2007.05a
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    • pp.1819-1823
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    • 2007
  • 유역에서 상류 유역 또는 수변을 따라 자연 자원을 이용하여 조성되어야 할 완충지대는 비점오염의 영향을 줄이기 위한 중요한 최적 관리공법이다. 교란된 토지 피복과 수변 완충지대를 관리 및 복원할 때 같은 지형조건이라도 식생 종류, 발달정도, 토양조건에 따라 오염물 제거효과가 다를 수 있으며, 최소 완충지대 길이도 제거 오염물질 대상별로 적합하게 설계되고 해석되어야 한다. 본 연구의 목적은 용담댐 유역에서 SWAT 모델을 이용하여 식생 완충지대의 비점오염원 저감 모의 재현성을 평가하는 것이였다. 소유역의 관측값과 모델 예측값에 대한 모델의 검 보정이 선행되었으며 그 중에서 가장 높은 모델 효율을 가지는 소유역에서 three zone concept의 90m 수변완충지대를 지방하천을 경계로 하여 토지 피복도를 변화시켜 SWAT 모델에 적용하였다. 이 때 교목으로는 포플러 (Populus, 30m)을 적용하였고, 포플러의 높이 및 Leaf area index를 조정하여 관목 지대(30m)에 입력하였다. 잔디 지대(30m)는 자연초지를 선택하였다. 또한 지형변수(average slope)와 Manning's coefficient을 수변완충지대의 조건에 고려하여 변화를 주었고 이에 따른 유출량 및 유사량, T-N, T-P의 변화율을 조사하였다. 또한 5, 10, 30m의 식생 여과대를 밭 지역에만 적용하여 오염물의 저감 효율을 평가하였다. SWAT 모델은 각 소유역에서 발생된 전반적인 유출량과 수질변수의 추세를 잘 예측하였으며 정자천 소유역이 선택되었다. 모의된 완충지대에서는 연평균 940 m3/ha의 유출량이 감소되는 것으로 유사농도는 28.7 %의 저감효과를 보였다. 그러나 T-N, T-P의 농도는 오히려 증가되었는데 본 연구에서 고려된 보다 수변완충지대를 조성할 때 지하수 수위, 식생 성장변화 등과 같은 보다 정확한 설계 인자 산출 및 모델 DB 개발이 유역모델에 포함되어야 할 것으로 판단되었다.한 해결책을 얻어내는 상호보완적인 결과를 추구한다. 그가 디자인하는 작품은 전형적인 이미지를 내포하지 않는다. 즉 그의 작품은 기존의 가치와 이념적인 것은 배제하고, 창의적인 개념을 도출하였다.형모서리는 건물 특화 성격이 강하므로 불가피할 경우 소형 액센트 광고 위치를 미리 벽면으로 할애하는 것이 경관 및 입면계획에 유리한 것으로 분석되었다. 불확실도 해석모형 등의 새로운 기능을 추가하여 제시하였다. 모든 입출력자료는 프로젝트 단위별로 운영되어 data의 관리가 손쉽도록 하였으며 결과를 DB에 저장하여 다른 모형에서도 적용할 수 있도록 하였다. 그리고 HyGIS-HMS 및 HyGIS-RAS 모형에서 강우-유출-하도 수리해석-범람해석 등이 일괄되게 하나의 시스템 내에서 구현될 수 있도록 하였다. 따라서 HyGIS와 통합된 수리, 수문모형은 국내 하천 및 유역에 적합한 시스템으로서 향후 HydroInformatics 구현을 염두에 둔 특화된 국내 수자원 분야 소프트웨어의 개발에 기본 토대를 제공할 것으로 판단된다.았다. 또한 저자들의 임상병리학적 연구결과가 다른 문헌에서 보고된 소아 신증후군의 연구결과와 큰 차이를 보이지 않음을 알 수 있었다. 자극에 차이가 있지 않나 추측되며 이에 관한 추후 연구가 요망된다. 총대장통과시간의 단축은 결장 분절 모두에서 줄어들어 나타났으나 좌측결장 통과시간의 감소 및 이로 인한 이 부위의 통과시간 비율의 저하가 가장 주요하였다. 이러한 결과는 차가운 생수 섭취가 주로 결장 근위부를 자극하는 효과를 발휘하는 것이 아닌가 해석된다. 이와 같은 연구결과를 통해 생다시마를 주원료로 개발된 생다시마차와 생다시마 음료가 만성 기능성 변비 증세를 개선하는 효능이 잠재적으로 있음을 확인하였다. 그러나 생약제재의 변비약 수준으로 변비 개선 효능을 증대하기 위해서는 재료 배합비의 개선이나 대장 운동기능을 향상시키는 유효성분의 보강 등이 필요하다는 점도 알 수 있었다.더불어 산화물질 해독에 관

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Flower bud development and the influence of sex expression of summer pruning in Schizandra chinensis Baillon (오미자의 화아(花芽) 발달과 성 발현에 미치는 하계전정의 영향)

  • Kim, Sun;Kim, Tai-Su;Park, Moon-Soo;Park, Ho-Ki
    • Korean Journal of Medicinal Crop Science
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    • v.11 no.2
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    • pp.97-101
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    • 2003
  • The purpose of this experiment is to improve the ratio of female flower in Schizandra chinensis Baillon. The experiment was carried out in two purposes. One was to understand the development stages on bud formation and determining sex after bud formation by stage. The other was to investigate characteristics of flowers forming on spring when summer pruning was taken place in different cutting part and timing in S. chinensis Baillon. The outcome was that on 7th of June the flower bud was found next to leaf primordium in branch of this year and no changes were found in shape for two weeks by 26th July. On 26th of June, an ovule that forms organ was observed and then, an ovule was found after sex was determined on 6th of July. Second experiment was carried out to find out when the sex was determined by adapting different pruning methods; cutting the branch at 6th node on June 1, June 15, and July 1; cutting the branch at 10th node on June 15, and July 1. The result was that the re-growth ratio on the branch 6th node was cut on June 1 and June 15. July 1 was 34-56% and, however, the number of setting flowers and mail flowers were fewer than the normal branches. More over, even though the re-growth ratio of branches were 34-25% when the cutting was taken place at 10th node on June 15 and July 1, the number of setting flowers and mail flowers were the same as the non treated branch. As a result, it is assumed that cutting controlling in this plant to increase the number of female flowers has no effect.