• Korean Journal of Animal Reproduction
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• 제25권2호
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• pp.101-111
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• 2001

The present study was conducted to develop an in vitro culture system that would support bovine follicle growth from preantral to antral stage, oocyte maturation, fertilization, and embryonic development. Bovine preantral follicles (150$\pm$1.2 ${\mu}{\textrm}{m}$) surrounded by theca cell were isolated ezymatically and mechanically from ovarian cortical slides in Leibovitz L-l5 medium containing 1 mg/$m\ell$ collagenase and 0.2 mg/$m\ell$ DNase I and cultured for 25 days in the presence of different concentrations of bovine FSH and LH in $\alpha$MEM medium. The survival and growth rates of follicles cultured in the presence of FSH (10~150 ng/$m\ell$) were significantly higher than those of control group (P ＜ 0.001), but no significant differences were observed in survival and growth rates of follicles between the LH treatment groups (1~125 ng/$m\ell$) and the control. The survival (40%) and growth (244 $\pm$ 0.5 $\mu\textrm{g}$) of follicles cultured with FSH (90 ng/$m\ell$) and LH (25 ng/$m\ell$) were higher than those of control (25%, 160 $\pm$1.0 $\mu\textrm{g}$). Finally, 50% percent of healthy antral follicles were obtained, and almost 60% of them has complete meiotic division with 1st polar body (18.1%) and 10.0% have developed to the cleaved embryo and blastocyst stage. These results suggest that bovine preantral follicle with intact theca cell can grow to the antral stage using these culture conditions, and that oocytes from in vitro-matured bovine preantral follicle may acquire meiotic competence and can undergo fertilization and development.

• KOREAN JOURNAL OF CROP SCIENCE
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• 제33권2호
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• pp.126-133
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• 1988

To know the optimum harvest time for seed yield and seed quality a local variety of rye 'Paldang-homil'was harvested at seven different harvest times from 25 to 55 day after heading (DAH) at five-day intervals in 1984 ani 1986. Seed development, seed germination and seedling growth were observed. The l000-grain weight increased as harvest time delayed until 50 DAH in both years. Although grain yield tended to increase with delay of harvest time, the yield differences between succeding harvest time was highest between 40 DAH and 45 DAH. Germination rate of seeds harvested before 30 DAH were lower than those after 35 DAH at 20 C, but at 10 and 30 C before 35 DAH were lower after 40 DAH. Plant height and dry weight of seedlings increased with delay of harvest time up to 45 DAH in pot. Heading stages were similar among the seeds harvested 40-55 DAH. Culm length was not different among the harvest times. The optimum harvest time for seed production of rye seems to be 45 DAH (38 days after flowering).

• Journal of the Society of Disaster Information
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• 제8권3호
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• pp.310-318
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• 2012

Actual condition by items based on the level of execution of Construction Company certified by Construction Safety and Health Management Systems (KOSHA 18001) was investigated, analyzed and evaluated reflecting various opinions fincluding safety experts, top management, audit experts, and construction engineers. Currently, the maintenance is being managed through internal audit after the safety and health management system has been certified, but it is difficult to identify the degree of continuous improvement. In order to present the standards to see the level of quantified system, this study was conducted. The purpose of this study is to present the system maturity evaluation tool to be used to reduce occupational accidents through proper establishment and continuous improvement of national health and safety management system. Results of this study are summarized through identification of current condition of implementation of KOSHA 18001 system, development of maturity measurement tool and verification as follows: First, priority of implementation for activities of headquarters and on-site was determined by importance of activities such as the risk assessment, safety and health accident prevention activities, performance assessment and monitoring, resource management and support, and management review and improvement in order. In addition, the expert group presented that association with continuous improvement activities could establish the system by presenting strengths, weaknesses and improvement subjects of system.

• KIPS Transactions on Computer and Communication Systems
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• 제7권7호
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• pp.165-174
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• 2018

The purpose of this study is to verify the effects of R&D process maturity on product development performance and to verify whether R&D project performance mediating effect. The questionnaire was collected and analyzed to 131 persons related to the R&D work in 55 companies and public institutions performing R&D work. The results of this study are as follows, the effect of R&D process maturity on product development performance and mediating effect of R&D project performance were analyzed by simple and multiple regression analysis. Regression analysis was analyzed by SPSS statistical program. The higher the maturity level of the R&D process, the higher the product development performance and the R&D project performance, and the R&D project performance is partially mediated. It is concluded that it is important to establish and operate a systematic R&D process and to develop a project management methodology suitable for the characteristics of a company in order to improve product development performance. It is expected that if this study are utilized as practical R&D management methodology in corporate management, it will contribute to improvement of company product performance.

• Korean Journal of Ichthyology
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• 제30권2호
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• pp.84-91
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• 2018

The study about maturity and spawning of Korean Mandarin fish, Siniperca scherzeri collected using the set net and gill net in Soyangho Lake from April to November 2014 was conducted. The number of individuals was 401 (female: 204, male: 197). The total length of females ranged from 113 mm to 365 mm and that of males was from 140 mm to 342 mm. The water temperature in May which is the start of spawning season indicated about $15^{\circ}C$ and gradually increased to July ($26^{\circ}C$). Gonadosomatic index (GSI) of females indicated 2.3%, 4.6% and 3.1%, respectively, in May to July. Males' value showed the similar pattern recording 8.0%, 6.6% and 4.5% in the same time. According to histological observations of gonadal tissue, the most of female in May had the gonadal tissue of maturing and mature stage. And the ovary of June was mostly in the stage of ripe and spawning and a number of individuals in July was recovery stage. In the case of male, a number of males in May showed in the stage of mature. The testis of June showed that mature sperm was releasing. And the testis of July was mostly in recovery stage. The total length at 50% group maturity was estimated 245.16 mm. As shown in the above, the main spawning period of S. scherzeri was May to June.

• Journal of Digital Convergence
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• 제14권9호
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• pp.571-577
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• 2016

Recently problems and issues of career-interrupted women were emerging as major social agenda in modern general and female society. This study modified and restructured REBT career group counseling and intended to testify the effects of REBT career group counseling program on self-efficacy and career attitude maturity of career discontinued married women in thirties. The subjects were career-interrupted married women in thirties living in J city, who have elementary school students as their children and applied to our study with 3 groups. 45 subjects were randomly assigned to experimental group(REBT Group), control group1(education group for vocational counselor) and control group2(general parental education group) by the number of 15. To assess effects of 3 groups, pre-test and post-test were carried out. Results showed that the participants of REBT group showed more self-efficacy, social efficacy and total self-efficacy scores than them of 2 control groups. And they had tendency to have more sense of reality, preparedness and total career attitude maturity scores. It seems that REBT career counseling program changed their irrational beliefs into rational beliefs about course and career, increasing their self-efficacy and career attitude maturity. The meaning, limitation and future tasks were discussed.

• The Korean Journal of Zoology
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• 제18권4호
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• pp.181-196
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• 1975

Rabbit follicular oocytes were cultured in a basic medium containing 0.4% bovine serum albumin (BSA), carbohydrates and amino acids in various combinations. Osmolarity of the medium was maintained at 308 mOsm. The carbohydrates, pyruvate, lactate and glucose were all about equally beneficial, but not essential for rabbit oocyte maturation. Glutamine and proline, but not methionine or phenylalanine stimulated oocyte develoment. Glutamine stimulated more follicular oocytes to develop to prophase and metaphase II than did any of the three carbohydrates tested alone or in combination. Ammonia production after 24 hours of culture was highest in medium containing glutamine(15.2$\\mu$g/ml) but this was not inhibitory to maturation. Negligible amounts of ammonia were found with the other amino acids added. The optimum level of osmolarity for rabbit oocyte maturation appears to be ranged from 250$\\sim$310 mOsm with the maximum level of 270 mOsm. With 0, 0.08, 0.4, 2, 10 and 50 mM of glutamine in the medium, plus BSA but without carbohydrates, 30, 73, 70, 71, 59, 45% of the oocytes developed to prophase or metaphase II respectively. This indicates that no carbohydrate is required of the maturation of rabbit oocytes when 0.08$\\sim$2 mM of glutamine is included, which are the optimum range. Follicular oocytes could develop in the medium containing $^14 C$-glutamine and BSA but without carbohydrates or other organic compound. From the $^14 CO_2$ produced and TCA precipitable material isolated, it is suggested that glutamine probably is utilized by oocytes and cumulus cells as a source of energy as well as for protein synthesis.

• Journal of Life Science
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• 제17권10호
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• pp.1347-1353
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• 2007

The changes of glycoconjuagates (GCs) in rat kidney due to maturation were studied from samples of fetal and postnatal kidneys by lectin histochemistry. Rat kidneys of perinatal ages and adults were fixed in 4% paraformaldehyde and were stained with nine kinds of biotinylated lectins. The immature forms of the renal developmental stage such as vesicles and ureteric bud were observed in the cortex as late as day 14 of postnatal life, but the histological appearance of the weaning kidney was similar to that observed in adults. As for histochemical properties of GCs in the glomeruli, Con A affinity tended to increase with aging, but both RCA-1 and LCA affinities showed a transient increase in immature glomeruli of neonatal rats. DBA affinity with SBA, PNA, BSL-1 and RCA-1, additional Con A one in proximal tubule, were increased in both proximal and distal tubules according to maturation. In contrast to this, transient intensive LCA affinity were demonstrated in immature proximal and distal tubule of neonatal rats. In the collecting tubules, DBA, SBA, PNA and sWGA affinities tended to increase according to maturation, but transient increase for BSL-1, RCA-1 and LCA affinities were detected in neonatal rats. The present results suggest that the mature glycosylation pattern of the kidney undergoes profound changes during maturation and is probably associated with functional maturation of the kidney.

• Informatization Policy
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• 제22권1호
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• pp.28-46
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• 2015

Although the demand for the use of public data increases in accordance with the expansion of Government 3.0, the poor level of data quality and its management currently implemented is becoming obstacles to opening data to the public. To improve the efficiency of management, linkage and usage for data, standardized processes for data quality management have to be prepared and appropriate data quality assessment criteria should be established. In this paper, we propose the organizational maturity model that can assess the public data quality management level. This model consists of the process reference model and the measurement framework. Fifteen processes grouped by the PDCA cycle are defined in the process reference model. The measurement framework measures the organizational maturity level based on process capability levels. The organizational maturity model can be used to establish objectives and directions for public data quality improvement by diagnosis of current level of public data quality management and problem solving. This model can also facilitate open to the private sector and activate usage of stable public data through reliability enhancement.

• Journal of the Korea Convergence Society
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• 제10권3호
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• pp.49-57
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• 2019

The core technology development of the Fourth Industrial Revolution is linked to the development of other core technologies, which will change the industrial structure in the future and create a new smart business model. In this paper, tried to analyze the technology maturity level and analyze the technology maturity. To do this, used technology trend information to investigate and integrate the market, policy, etc. Of core technology of the 4th Industrial Revolution to achieve a comprehensive maturity level. Because technology maturity measures are scored by technology developers, prejudices may be acted upon according to a person's tendency, which may be a subjective evaluation. It is also a measure of the maturity of individual technologies, and thus is not suitable for evaluating the overall system integration perspective. However, it is possible to evaluate the maturity before integrating the core element technologies constituting the whole system and to use it as a means to compare the effect of the whole system and its feasibility and play an important role in the planning of technology development.