• Journal of Embryo Transfer
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• v.21 no.1
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• pp.53-58
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• 2006

This study was conducted to comparing on vitrification of mouse oocytes and embryos using CPS, conventional straws and CPS by evaluating in morphological survival for oocytes, and embryonic cleavages and blastocyst formation for embryos. The morphological survival in vitro after thawing of vitrified oocytes using CPS (75%) and conventional straws (72%) were significantly higher (p<0.05) than that using OPS (68%). The blastocyst formation rates of vitrified embryos using CPS (48.6%) and unfrozen control embryos (56.0%) were significantly higher (p<0.05) than those of conventional straws (43.4%) and OPS (37.7%). The rates of morula formation were also higher to control, CPS, conventional straws and OPS in orderly. These results show that CPS has the advantages of achieving a high survival and safety preservation.

• Korean Journal of Environmental Biology
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• v.20 no.4
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• pp.386-390
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• 2002

The opening phenomenon of mature female cone in Pinus densiflora aye observed from the anatomical characteristics. The differentiation of tissue in young cone aye not complete and are going on. In the green cone as large as the adult cone, shrink-age rate of vascular strands and sclerenchyma aye 1.0∼1.5％ and 14.0∼16.0% respectively. The end parts of cone scales are composed of the parenchymatous cell and sclerenchyma. The middle parts in cone scales are composed of the sclerenchyma and vascular strands with the same thickness and more thin than the thickness of the end part and basal one. But thickness of sclerenchyma which compose of scale prop in the basal part of cone scale aye twice than that of vascular strand. In mature cone, the opening phenomenon of the pine cone results from the difference of shrinkage rate between vascular bundle and sclerenchyma. Especially, opening of cone scales are due to larger shrinkage rate of sclerenchyma than vascular strands in the basal part of cone scale.

• The Korean Journal of Financial Management
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• v.17 no.1
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• pp.203-226
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• 2000

본 연구는 주가지수 선물시장이 도입된 1996년 5월 3일부터 1998년 12월 5일까지 1분 간격 KOSPI 200 선물가격과 현물가격의 거래자료를 이용하여 각 선물가격과 기초자산가격간의 관계와 상호작용을 검토하는데 있다. 특히 본 연구는 차익거래자나 초단기 투기자(scalper)들이 거래체결을 위해 촌각을 다투는 선물시장의 거래행태에서 볼 때, 경제적 의미를 부여할 수 있는 1분 간격 수익률 자료를 이용함으로써 시장참여자의 실제 거래에서 표출되는 정형화된 현상을 정확히 파악한다는 점에서 중요하다. 본 연구의 주요 결과를 제시하면 다음과 같다. 첫째, 주가지수 선물시장과 현물시장간에 체계적이고 긴 선도-지연 관계가 발견되었다. 주가지수 선물가격의 변화가 현물가격의 변화를 대략 26분 정도 선도하고 있으며, 대략 5분 정도 현물시장의 선도효과도 발견된다. 따라서 KOSPI 200 선물수익률과 현물수익률간의 선도-지연 관계는 한 시장에서 다른 시장으로의 일방적인 것이 아니라 시장간의 피드백(feedback)효과가 존재하며, 선물의 선도효과가 지배적인 것으로 보인다. 이러한 선도-지연 현상은 노이즈에 의한 비동시거래보다는 거래비용과 공매제약 차이 등 각 시장의 제도적 차이에 의해 발생하는 것으로 보여진다. 둘째, 약세시장 하에서 선물의 선도효과가 더욱 크게 나타났다. 이러한 현상은 약세시장 하에서 현물시장의 공매제약이 선물가격과 현물가격간의 괴리를 더욱 크게 하여 선물가격이 현물지수를 더욱 선도하게 하는 요인이 될 수도 있음을 나타내는 것이다. 셋째, 만기별 하위기간 중 97년 6월과 97년 12월을 제외한 기간은 선물과 현물가격간에 장기 안정적인 균형관계가 성립함을 발견하였다. 넷째, ARMA(p, q) 여과를 거친 선물과 현물수익률을 이용하여 97년 6월과 12월은 백터자기회귀(VAR)모형, 그 외의 기간은 오차수정(EC)모형으로 추정하였다. 표본전체기간동안 장기균형오차에 대한 조정은 선물과 현물시장에서 동시에 이루어지고 있으며, 시장간에 발생하는 불균형 상황은 아비트라지 거래로 조정되고 있음이 발견되었다. 각 만기별 모든 하위기간에 있어서는 시장간의 장기 불균형 상황이 현물시장을 통해서 조정되고 있으며, 시장이 성숙된 최근의 만기 98년 12월 하위기간에서는 선물의 15분 선도효과와 현물의 1분 선도효과가 발견되어 선물의 선도효과가 지배적임을 발견하였다.

• The Korean Journal of Malacology
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• v.32 no.2
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• pp.67-71
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• 2016

In order to obtain a large number of fertilized eggs for seedling production, experiment was carried out examine effects of serotonin on spawning of the Pacific oyster Crassostrea gigas. The shorter response time to initial spawning in case of serotonin injection showed, the higher serotonin injected with 7.6-27 min. The response time to initial sperm releasing showed the same tendency with female. The highest response rates and eggs amount spawned were showed in the highest concentration. The serotonin injection had no effect on frequency of germinal vesicle breakdown (GVBD), fertilization and hatching rate.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.19 no.2
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• pp.155-168
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• 1986

In Korea, lots of Israeli carp (Cyprinus carpio) are cultured by means of net cages in dams and lakes, but many carp have been subjected to heavy infestation of the cestoid, Bothriocephalus opsariichthydis. Nevertheless the parasitic state and life history of the cestoid are not yet reported. This reason led the author to study the parasitic state and life history of B. opsariichthydis parasitized in the carp in order to take effective control measures against its damage. Israeli carp were sampled from two fish farms, in Taech'on and Kyongch'on. After dissection of the specimens, the cestoid were obtained and the parasitic rates were examined. After taking the eggs from adult worms, the development of the eggs were observed. Coracidia were exposed to four kinds of crustaceans in order to investigate the infection rate and development of the larva. Finally, tile development of the larva in the final host was investigated. The fully mature eggs were in the cleavage stage, when they are released, and the size ranged 47.5 to 55.0 $(50.9){\times}30.0$ to 32.5 (31.1) um, in the state of under-developed coracidia and blastomeres. The parasitic rate of the cestoid in Israeli carp from Taechon was $55.5\%$ in 1984 and $21.6\%$ in 1985, that from Kyongchon was $64.7\%$ in 1984, and color carp from Kusan was $14.9\%$ in 1984. The eggs were hatched to coracidia within 48 hours under 26 to $28^{\circ}C$. The cestoid showed a strong affinity to Thermocyclops hyalinus and Paracyclops fimbriatus and the infection rates were $93.5\%$ and $75.5\%$, respectively. At 14 days after the infection to Thermocyclops hyalinus and Paracyclops fimbriatus, the larvae of the cestoid grew into fully developed procercoids; 207 to $226 (214){\times}90$ to 102 (94) um in size. Sixty days after carp have ingested the Thermocyclops hyalinus infected with the fully developed procercoids, the larvae of the cestoid matured into adult worms in the intestines of the carp.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.86-86
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• 2002

면양과 염소가 최근 수십년동안 세계여러 나라에서 번식생리의 연구를 위한 모델로 사용되어 왔는데, 체내수정란의 생산에 관한 영역도 유럽을 중심으로 활발하게 연구되어왔다. 수정란생산을 위한 발정동기화방법, 과배란처리 및 수정란회수방법 기술은 현재 상당히 많은 기술진척이 이루어진 상태이나, 우리나라 고유의 재래유전자원인 흑염소에는 이를 위한 기술이 미진한 실정이므로 본 실험에서는 흑염소의 체내수정란생산기술을 확립하여 재래가축 유전자원보존을 위한 기초기술을 마련하고자 한다. 축산기술연구소 남원지소에서 사육하고 있는 체중 20kg 이상의 건강한 흑염소를 이용하여 발정동기화를 위해 controlled intravaginal drug release(CIDR)를 질내에 14일 동안 삽입하고, 과배란처리는 FSH를 CIDR 삽입 12, 13, 14일째에 12시간 간격으로 점감법으로 총20mg을 투여하였으며, PGF$_2$a를 13일째 FSH와 함께 투여하였다. CIDR는 14일째의 아침에 제거하였다. 수컷과의 교미는 CIDR제거 24시간후에 GnRH를 투여와 동시에 실시하였으며, 채란은 교미후 3일째에 외과적인 방법으로 실시하였다. CIDR처리경과에 따른 progesterone농도는 CIDR 주입시 바로 수치가 상승하여 제거전까지 6~12ng/m1의 농도를 유지하였으며, 제거즉시 2ng/ml 이하로 떨어졌다. 채란시 평균 배란점은 16.5개, 미배란난포 9.8개였으며, 회수수정란은 6.0개를 나타내어 채란율은 36.4%를 나타내었다. 회수된 수정란의 발달단계는 4-cell 78.9%, 2-cell 5.3%, fragmentation 15.8%를 나타내었다. 이와 같은 체내수정란생산방법을 기반으로 하여 이후 수정란의 동결 및 수정란이식기법에 관한 연구를 수행한다면 우리나라의 재래가축인 흑염소의 유전자원 장기보존과 생산성향상에 기여할 것으로 사료된다.배양액에 30 embryos/50ul 소적으로하여 38.8$^{\circ}C$, 5% $CO_2$의 탄산가스 배양기에서 각각 7일간 배양을 실시하였다. 조사된 결과는 SAS/STAT를 이용하여 통계분석을 실시하였다. 체외수정 12시간 후에 난자 급속 염색법으로 염색을 실시한 결과, 모든 처리구에서 핵성숙률(76.4~95.2%), 정자침투율(51.1~66.9%), 웅성전핵형성률(95.2~100%), 다정자침입률(18.2~25.6%) 및 평균침입정자수(1.2~l.4개)에서 유의적인 차이가 인정되지 않았다. 체외배양 48시간 난할률을 조사한 결과, 처리구별 차이(53.9~67.9%)는 인정되지 않았으나, 배양 7일째 배반포형성률은 각각 14.5, 25.4, 17.3 및 12.4%로서 25uM의 $\beta$-ME처리구가 유의적(P<0.05)으로 높은 배발달률을 나타내었고, 총세포수에 있어서는 대조구와 처리구간 유의적인 차이가 인정되지 않았다. 따라서 돼지 난포란을 성숙배양할 때, 25uM $\beta$-ME를 첨가배양하는 것이 양질의 돼지체외수정란을 생산하는 하나의 방법으로 조사되었다.다.natural objects and was popular at the time of Yukjo Dynasty, and there are some documents of that period left both in Japan and Korea. "Hyojedo" in Korea is supposed to have been influenced by the letter design. Asite- is also considered to have been "Japanese Letter Jobcheso." Therefore, the purpose of this study is to look into the origin

• The Korean Journal of Zoology
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• v.30 no.1
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• pp.10-28
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• 1987

Changes of protein synthesis in the isolated egg chambers were studied during oogenesis of Drosophila melanogaster. Protein synthesis did not change much in quantity except that the activity was slightly enhanced at the stages 11 and 12, when new structures such as vitelline membranes and yolk granules were made, but considerably changed in quality during oogenesis. Protein synthesis was believed to occur mainly in the follicle cells, and a number of stage-specific proteins were found to differ at various stages. The observations suggest that proteins synthesized in the cells within chambers as well as those from the outside of the chambers might be directly required for the growth and maturation of oocytes in Drosophila.

• Korean journal of applied entomology
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• v.59 no.4
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• pp.427-432
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• 2020

The ability of sunflower, bean, green perilla, and sesame to attract Metcalfa pruinosa was tested in both laboratory and field conditions. In the laboratory experiment, sunflowers showed the highest attractiveness to M. pruinosa nymphs and adults. These results were the same as those in the field experiment. Sunflowers showed the highest attractiveness among the four candidates as a trap plant for M. pruinosa, with comparable average attractiveness values for nymphs and adults to those in the laboratory. Young beans also showed high attractiveness to M. pruinosa, albeit lower than those of sunflowers. However, the attractiveness of mature beans was low. Sunflowers consistently (P < 0.05) showed significantly higher attractiveness than that of the other three plants, regardless of plant age. Thus, sunflowers would serve as a good trap plant for both the nymphs and adults of M. pruinosa.

• Journal of Life Science
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• v.26 no.12
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• pp.1466-1469
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• 2016

A group of traditional folk medicines obtained from the fruits of Rutaceae plants, such as Ponciri Fructus Immaturus (PFI) from Poncirus trifoliata Raf., Aurantii Fructus Immaturus (AFI) and Aurantii Pericarpium (AP) from Citrus aurantium L., and Aurantii Nobilis Pericarpium (ANP) from Citrus unshiu Marc., has been widely used for improving poor gastrointestinal function in East Asia, including in China and Korea. In the present study, the effects of Rutaceae-fruit-derived folk medicines on gastrointestinal motor function were investigated and compared by measuring in vivo gastric emptying and intestinal transit in mice. Among the aqueous and ethanolic extracts of some Rutaceae-fruit-derived folk medicines, an ethanolic extract of AP (AP-E) significantly accelerated intestinal transit in a dose-dependent manner without affecting gastric emptying. Furthermore, the intestinal transit rate of AP-E was the highest among all extracts examined in this study, including aqueous extracts of PFI, AFI, and ANP, of which the activities of accelerating intestinal transit have been already reported.

• The Korean Journal of Malacology
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• v.26 no.3
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• pp.201-209
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• 2010

The investigated of survival rate, sex ratio, condition index, gonad development frequency, gonad index, fertilization rate, hatching rate and time required development of D shape larvae of the ark shell, Scapharca subcrenata. Survival rate was lower in heating group compared with control group (P < 0.05). Sex ratio (female:male) observed 1:0.97 in control group, 1:0.62 in heating group. Condition index was significantly higher in heating group than control group (P < 0.05) and higher increased more than water temperature $17^{\circ}C$ of heating group. Gonad development observed slow in control group but more than ripe stage 80% in heating group (21-28 day, $20^{\circ}C$). Gonad index was higher in heating group compared with control group (P < 0.05). Fertilization rate and hatching rate of the ark shell was observed $82.17{\pm}3.55%$ and $63.57{\pm}2.36%$, respectively. The time required development of D shape larvae of the ark shell was observed 15 hour after fertilization. The suggested that results of the ark shell by heating were effective sex maturation and normal egg development.