• Journal of Animal Science and Technology
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• v.47 no.4
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• pp.607-614
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• 2005

To determine the optimum fermentation condition, the 50:50 feed mixture of both crushed corn and wet brewer’s grain were anaerobically fermented at various conditions. Three supplementation levels(0, 2%, and 5%) of molasses, five supplementation levels(0, 0.1%, 0.5%, 1.0%, and 2.0%) of yeast and five different incubation temperatures(4$^{\circ}C$, 10$^{\circ}C$, 30$^{\circ}C$, 40$^{\circ}C$ and 50$^{\circ}C$) were tested to determine the optimum fermentation conditions. During fermentation, alcohol concentration, live yeast cell number (LYCN) and pH values of the mixture were analyzed. Alcohol concentrations of the fermented feed mixtures were proportionally increased with increasing level of molasses supplementation. After 24hr fermentation, alcohol concentrations at 0.1%, 0.5%, 1.0% and 2.0% of yeast supplementation was 1.9%, 2.4%, 3.1% and 3.1%, respectively. These results indicate a proportional increase of alcohol concentration with the increasing supplementation of yeast (P<0.05) up to 1%, thereafter showing plateau at 2% supplementation. LYCN was relatively high with more than 0.5% yeast supplementation, whereas the value was significantly low(P<0.05) at 0.1% yeast supplementation. Alcohol production was significantly higher at 30$^{\circ}C$ and 40$^{\circ}C$ than other incubation temperatures (P<0.05), with the highest concentration at 30$^{\circ}C$. pH was rapidly decreased until 24 hours of incubation with steeper decrease at lower temperatures(P<0.05). Based on there results, optimum fermentation conditions for the alcoholic-fermented feed production could be achieved with 5% supplementation of molasses, 1% supplementation of yeast, and 24hr long incubation at 30$^{\circ}C$.

• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.1
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• pp.45-51
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• 2013

This study was performed on two groups (10 cows) for primiparous Holstein lactating cows (av. 98 days in milk : DIM) which were divided into low forage diet (LF) and high forage diet (HF) groups based on forage : concentrate ratio (F : C ratio). The F : C ratios of LF and HF groups were 37:63 and 62:38, respectively. Concentrate intake was significantly higher in the LF group than the HF group whereas the HF group showed higher forage intake (12.9 kg) compared to the LF group (7.4 kg) (p<0.05). No significant difference was observed in total feed intake between the HF (20.9 kg) and LF (19.4 kg) group (p>0.05), but the HF group tended to be higher. CP, TDN and NEL intake showed no significant difference between the two groups (p>0.05). Though, there was no significant difference on actual milk between the two groups (26 vs. 23.9 kg/d, p>0.05), the LF group tended to be higher. 4% FCMs of LF and HF groups were 22.8 and 22.3 kg/d, respectively, and showed no significant difference (p>0.05). The HF group was higher in fat content and lower in MUN. C14:0, C16:0 and C16:1n7 of milk fatty acid were significantly higher in the HF group (p<0.05), but there were no differences in other milk fatty acids between the two groups. As a result, increasing high quality forage such as BIRG silage and hay in the diet will not only fulfill nutrient requirements but also reduce milk production.

• Korean Journal of Clinical Laboratory Science
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• v.49 no.4
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• pp.446-454
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• 2017

Human papillomaviruses (HPVs) are major causes of cervical cancer. Sixteen high risk HPVs, including HPV 16, HPV 18, HPV31, HPV 33, HPV 35, HPV 39, HPV 45, HPV 51, HPV 52, HPV 53, HPV 56, HPV 58, HPV 59, HPV 66, HPV 68, and HPV 69 are found in cervical cancer. HPVs 16 and 18 are mainly presented in 70% of cervical cancer. Therefore, identifying the presence of these high-risk HPVs is crucial. The objective of this study is to establish the HPV ViroCheck for detecting 16 HR-HPVs and genotypes of HPVs 16 and 18, as well as to analyze the analytical performance of HPV ViroCheck. We performed the analytical sensitivity of HPV E6 / E7 genes of 16 high risk HPVs to confirm the limit of detection. Then, a cross reactivity of HPV ViroCheck with microorganisms and viruses related to the cervix were analyzed for analytical specificity. Analytical sensitivity of high risk HPV genotypes ranged from 1 to 100 copies when using cloned DNAs. The limit of detection was 10 cells for both SiHa and HeLa cells. Cervical-related microorganisms and viruses did not show cross-reactivity to HPV DNA. Moreover, the intra- and inter-assay coefficient variations (CVs) were below 5%. In conclusion, HPV Virocheck will be useful for the detection of 16 HR HPVs, as well as HPV 16 and HPV 18 genotypes rapidly.

• Journal of Animal Science and Technology
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• v.47 no.5
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• pp.759-768
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• 2005

The study was designed to estimate the in vitro rumen by-pass rate of both chromium methionine chelate as an organic supplement and $ClCl_3$ as an inorganic supplement. Rumen by-pass rates of the supplements were evaluted by comparing ruminal metabolites in rumen fluid and Cr and methionine contents in the body of ruminal microorganism. For in vitro digestion examination, basic nutrients for ruminal microbes were supplied with 7g(DM) of feed, 2g of rice straw, and 2g of corn silage per each incubation jar. Three treatments including Control(no supplementation of Cr), T1(1000ppb supplementation of $ClCl_3$) and T2(chromium methionine chelate supplementation equivalent to 1000ppb of Cr content) were prepared with five replications per each treatment. pH of T2 was lower than that of Control and T1 regardless of incubation time. Ammonia content was higher in T2 than in Control and T1 during first 6 hours of incubation. However, the ammonia content in Control was remained low after 6 hours. Total volatile fatty acids(VFA) content in control was increased constantly as incubation time was extended. Therefore, VFA content in T1 and T2 were significantly lower (P<0.05) than those of Control. Dry matter recovery rate by ruminal microorganism was the lowest in T1, however ruminal microbial population was increased most efficiently in T2 during 12 hours of in vitro incubation. Cr concentrations in the body of ruminal microbes were not different(P>0.05) between Control and T2, but it was significantly high in T1(P<0.05). Contents of methionine and cystine in ruminal microbes also were not different between Control and T2(P>0.05), but it was relatively low in T1. Based on the above results, the chromium methionine chelate was believed to by-pass rumen and could remain intact until it reaches small intestine compared to inorganic chromium. This results implies that chromium methionine chelate could be more effective to function in the small intestine of ruminant animals.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.4
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• pp.239-244
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• 2005

This study was conducted to investigate the effect of the planting densities and nitrogen levels on the growth characteristics, dry matter yield and nutritive value of corn for silage in alpine area(altitude 800m a.s.1.) of National Livestock Research Institute from 2001 to 2002. The experiment was arranged in a split plot design with three replications. Main plots consisted of three planting densities, low(67,000 plants/ha), medium(89,000 plants/ha) and high(95,000 plants/ha). Sub plots consisted of three nitrogen levels, 150 kg/ha, 200 kg/ha, and 250 kg/ha. Tassel height of corn was increased with decreasing planting density, and nitrogen level of 200 kg/ha in all main plots. Ear height of corn was decreased with increasing planting density, and 150 kg/ha of three nitrogen levels. But there were no significant differences among treatment in tassel and ear height. Although dry matter ear ratio of whole com was increased with decreasing planting density, there were no significant differences among treatments. But there were significant differences among nitrogen levels(P<0.05). Dry matter yield of com was high with increasing planting density, but there were no significant differences among treatments. And DM yield of corn was significant difference among nitrogen levels(P<0.05). Crude protein content of corn was the highest with high planting density and low nitrogen level. NDF and ADF content of com was the highest with low planting density and nitrogen levels of 200 kg/ha in all main treatments. These results indicate that low planting density(67.000 plants/ha) and nitrogen of 150-200 kg/ha would be the optimum level fur dry matter yield and nutritive value of silage com in alpine area.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.4
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• pp.259-266
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• 2005

The objective of this study was to determine which mixture types are suitable for maintaining grazing pasture at alpine area. The mixtures types were evaluated by the botanical composition and dry matter (DM) of forage. The experiment was carried out on the grazing pasture at Daekwanryeong area (altitude ; 840m) from 1991 to 1993. Treatments were composed of seven mixture types made of both different components and amounts of sowing. The components of each mixture were Orchardgrass (OG), Tall fescue (TF), Timothy (TI). Kentucky bluegrass (KBG), Reed cannarygrass (RCG), Red clover (RC) and Ladino clove. (LC). The seeding .ate of OG, TF, TI, KBG and LC in control(C) was 18:9:8:3:2 kg/ha, respectively and those of treatments of T1 (OG:TF:KBG:LC), T2 (OG:TF:KBG:RC), T3 (OG:TI:KBG:LC), T4 (OG:TF:KBG:LC), T5 (OG:TI:KBG:LC) and T6 (OG:RCG:KBG:LC) were 24:8:2:2, 24:8:2:2, 26:8:2:2, 8:28:2:2, 5:24:2:2 and 8:26:2:2 kg/ha, in seeding rate. respectively. The plant heights of mixtures in 1991, 1992 and 1993 were 33, 37, and 33 cm on average of before and after grazing, respectively. The 12cm of stubble height was left after grazing during 3 years which can result in the reduction of forage intake and utilization. This result indicates the grazing period will be needed to adjust. The proportions of TI, OG, KBG and LC in mixtures were increased constantly, and the proportions of weeds and bare lands were little. The T5, in which the smaller amounts of seeds were sown, is considered as proper mixture type for Alpine pasture. The DM yield tended to increase with the increased grazing period, and the highest DM yield was occurred in 1993. No significant difference among treatments was observed for the DM yield, however the 75 was highest (9,344 kg/ha) in the DM yields which is $5.7\%$ greater than the C (8,840 kg/ha). This result indicates that T5 (OG:TI:KBG:LC : 8:24:2:2 kg/ha) is considered to be proper mixture type at alpine pasture considering the botanical composition and forage yield.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.1
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• pp.43-50
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• 2005

The purpose of this study is to discuss the ways to consistently feed high quality corn silage(CS). This study evaluated the effect of the corn silage, after a certain time has elapsed, on the chemical composition and fermentation characteristics after feedout during the winter feeding period of the CS. Six samples of CS from four dairy farms(E1, E2, E3, and L1 ) were taken in order to feed the milking cows over a winter feeding Period from November of 2002 until February of the following year, 2003(six samples were taken at the fellowing dates in the following order: sample one was taken on the 23rd Nov. 2002, sample two on the 5th of Dec. 2002, 3rd sample on the 23rd of Dec. 2002, 4th sample on the 7th of Jan. 2003, 5th sample on the 22nd of Jan. 2003, and the 6th sampling was carried out on the 6th of Feb. 2003) at the three sampling sites after the opening of the trench silos at intervals of 15 days. In the dry matter contents of CS, there wasn't any specific tendency according to the elapsed time in the range of 21.3~$27.3\%$ at all low dairy farm(E1, E2, E3, and L1). And the average dry matter contents were 24.1, 25.9, 23.6, and $20.4\%$. Considering the Proper amount of the dry matter of CS during the ripen yellow stage, the appropriate moisture content was $33\%$ (NRC, 1989), and these dry matter contents were all low. A consistent tendency was not found in the contents of CS. The average of CP contents were 10.2, 8.0, 8.5, and $9.8\%$ at the E1, E2, E3, and L1 farms, and there were significant differences. The TDN contents of CS were not different among forms according to the time elapsed. The pH, according to the time elapsed after opening of the CS, there were no differences at each of E1, E2, E3, md L1 farms. Average pH were 3.5, 3.9, 3.6, and 4.1, md all of them were in normal range. In the lactic acid contents of CS, a consistent tendency was not found among four farms. But according to the time elapsed. there was a goat difference from 1.13~$7.8\%$ The acetate, propionate, and butyrate contents of CS were very low. In this study, there was no significant difference in the CS's chemical composition and fermentation characteristics according to the elapsed time at all four dairy farms. Considering the proper dry matter contents of CS during the ripen yellow stage, the appropriate dry matter content was $33\%$, and dry matter contents of few farms were all low. To enhance the quality of corn silage should be ensiled com at proper dry matter content range from 28 to $35\%$ Therefore, content of the corn plant should be always be closely monitored prior to beginning harvest.

• Journal of The Korean Society of Grassland and Forage Science
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• v.32 no.4
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• pp.437-446
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• 2012

As a part of establishing suitability classification for forage production, use of the national soil and climate database was attempted for Italian ryegrass (Lolium multiflorum Lam., IRG) in Gangwon Province. The soil data base were from Heugtoram of the National Academy of Agricultural Science, and the climate data base were from the National Center for Agro-Meteorology, respectively. Soil physical properties including soil texture, drainage, slope available depth and surface rock contents, and soil chemical properties including soil acidity and salinity, organic matter content were selected as soil factors. The crieria and weighting factors of these elements were scored. Climate factors including average daily minimum temperature, average temperature from March to May, the number of days of which average temperature was higher than $5^{\circ}C$ from September to December, the number of days of precipitation and its amount from October to May of the following year were selected, and criteria and weighting factors were scored. The electronic maps were developed with these scores using the national data base of soil and climate. Based on soil scores, the area of Goseong, Sogcho, Gangreung, and Samcheog in east coastal region with gentle slope were classified as the possible and/or the proper area for IRG cultivation in Gangwon Province. The lands with gentle or moderate slope of Cheolwon, Yanggu, Chuncheon, Hweongseong, Pyungchang and Jeongsun in west side slope of Taebaeg mountains were classified as the possible and/or proper area as well. Based on climate score, the east coastal area of Goseong, Sogcho, Yangyang, Gangreung and Samcheog could be classified as the possible or proper area. Most area located on west side of the Taebaeg mountains were classified as not suitable for IRG production. In scattered area in Chuncheon and Weonju, where the scores exceeded 60, the IRG cultivation should be carefully managed for good production. For better application of electronic maps.

• Journal of The Korean Society of Grassland and Forage Science
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• v.37 no.2
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• pp.145-153
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• 2017

This study was aimed to find yield prediction model of Italian ryegrass using climate big data and geographic information. After that, mapping the predicted yield results using Geographic Information System (GIS) as follows; First, forage data were collected; second, the climate information, which was matched with forage data according to year and location, was gathered from the Korean Metrology Administration (KMA) as big data; third, the climate layers used for GIS were constructed; fourth, the yield prediction equation was estimated for the climate layers. Finally, the prediction model was evaluated in aspect of fitness and accuracy. As a result, the fitness of the model ($R^2$) was between 27% to 95% in relation to cultivated locations. In Suwon (n=321), the model was; DMY = 158.63AGD -8.82AAT +169.09SGD - 8.03SAT +184.59SRD -13,352.24 (DMY: Dry Matter Yield, AGD: Autumnal Growing Days, SGD: Spring Growing Days, SAT: Spring Accumulated Temperature, SRD: Spring Rainfall Days). Furthermore, DMY was predicted as $9,790{\pm}120$ (kg/ha) for the mean DMY(9,790 kg/ha). During mapping, the yield of inland areas were relatively greater than that of coastal areas except of Jeju Island, furthermore, northeastern areas, which was mountainous, had lain no cultivations due to weak cold tolerance. In this study, even though the yield prediction modeling and mapping were only performed in several particular locations limited to the data situation as a startup research in the Republic of Korea.

• Journal of The Korean Society of Grassland and Forage Science
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• v.40 no.1
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• pp.29-36
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• 2020

This study was conducted to suggest the new grassland grade system on evaluating the grassland status. The grassland status has been evaluated based on the forage yield (good, fair and poor) by municipal authorities. The grassland grades by current system were 19 good, 11 fair and 11 poor among the 41 grassland farms from 6 provinces. This evaluation result differed greatly from the result of actual measurement of forage yields which showed all poor. The big difference was resulted from failing the reflection of the various characteristics, such as different seasonal growth and harvest frequency. Furthermore, the lack of consistent examining date and method added the inaccuracy of current grassland grade system. The new grassland grade system based on the grassland vegetation ratio (grass, weed and bare soil) was initially designed into 6-grade system (1st; 100~80%, 2nd; 79~60%, 3rd; 59~40%, 4th; 39~20%; 5th; 19~1% and 6th; 0% on the basis of grasses proportion), but later was changed into 4-grade system (1st, 2nd, 3rd, and 4th grades are 70% or more, 50% or more, 50% or less, and 0% of forage proportion, respectively) after reflecting the opinion of grassland farms and municipal authorities. Re-evaluation on the grassland status using the 4-grade system resulted in the total 80% consisted of 2nd, 3rd and 4th grade which means most grasslands needs the partial reseeding or the rehabilitation of entire grassland. Pictures and schematic diagrams depicting the 4-grade system were presented to improve the objectivity of evaluation. The optimal time for assessing grassland status is fall when plant height 20~30 cm. Conclusively, the 4-grade system is an efficient method for all non-professionals including grassland farms or municipal authorities in assessing the grassland status. To apply this system to the field, the institutional arrangements such as amendment of grassland act should take place in advance.