• Title/Summary/Keyword: 섬유소 용해

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Evaluation of nutritive value of chestnut hull for ruminant animals using in vitro rumen fermentation (밤 가공 부산물의 반추가축용 사료 가치 평가: in vitro 반추위 배양)

  • Jeong, Sin-Yong;Jo, Hyeon-Seon;Park, Gi-Su;Kang, Gil-Nam;Jo, Nam-Chul;Seo, Seongwon
    • Korean Journal of Agricultural Science
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    • v.39 no.3
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    • pp.335-340
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    • 2012
  • During the manufacturing process of chestnut, 50% of biomass is produced as chestnut shell (CS) or chestnut hull (CH), a forestry by-product. Due to its high fiber content and economic benefit, there is a possibility of using chestnut hull as a supplement for a ruminant diet. Few studies, however, have been conducted on evaluating nutritive value of chestnut hull for ruminant animals. The objective of this study were thus to analyze chemical composition of CS, a by-product after the first processing of chestnut, and CH, a by-product after the second processing, and access in vitro rumen fermentation characteristics of them. For the in vitro fermentation using strained rumen fluid obtained from a fistulated Hanwoo steer, commercial total mixed ration (TMR) for dairy goat was used as a basal diet and was replaced with different proportions of chestnut shell and hull. A total number of 13 treatments were carried out in this study: 100% TMR, 100% CS, 100% CH, a mix with 50% CS and 50% of CH (MIX), TMR replaced with 5%, 10%, or 15% of CS, CH, or MIX, respectively. For each treatment, in vitro dry matter digestibility (IVDMD) and pH after 48 hours of rumen fermentation were measured. Gas production at 6, 12, 24, 48 hours of incubation was also analyzed. Compared to CH, CS contains higher level of fiber (NDF, ADF, lignin) and consequently has a lower amount of non-fiber carbohydrate, but no difference was observed in the other nutrients (i.e. crude protein, crude fat, and ash). IVDMD was significantly (p<0.05) the highest in 100% CH (71.97%) and the lowest in 100% CS (42.80%). Addition of CH by replacing TMR did not affect IVDMD, while an increase in the proportion of CS tended to decrease IVDMD. The total gas production after 48 hours of incubation and the rate of gas production were also the highest in 100% CH and the lowest in 100% CS (P<0.05). Likewise, the pH after 48 hours of fermentation was significantly (p<0.05) the lowest in 100% CH (6.33) and the highest in 100% CS (6.50), and no significant difference in gas production was observed when TMR was replaced with CS or CH up to 15% (P>0.05). In conclusion, CH may successfully be used for a supplement in a ruminant diet. The nutritive value of CS is relative low, but can replace, if not 100%, low quality forage. This study provides valuable information about the nutritive value of CS and CH. An in vivo trials, however, is needed for conclusively accessing the nutritive value of CS and CH.

Estimation of Availability of Whole Crop Barley and Rye Silage TMR in the Cattle (청보리 및 호밀 Silage를 이용한 거세한우 비육중기용 TMR의 소 체내 이용성 조사)

  • Qin, Wei-Ze;Jin, Guang Lin;Kim, Jong-Kyu;Oh, Young-Kyoon;Lee, Sang-Cheol;Song, Man-Kang
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.30 no.4
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    • pp.343-354
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    • 2010
  • The present study was conducted to examine the fermentation characteristics and effective degradability (ED) in the rumen, and whole tract digestibility of whole crop silage based TMR in comparison with conventional separate feeding of concentrate and roughage. Three ruminally fistulated non-lactating Holstein cattle were used in a 3 $\times$ 3 Latin square design. The cattle were fed 8kg of whole crop barley silage based TMR (BS-TMR) or 8kg (DM basis) of whole crop rye silage based TMR (RS-TMR) twice (08:00 and 18:00) daily in an equal amount. The cattle were also fed concentrate (5.6kg) and rice straw (1.4kg) seperately (DM basis, Control) twice daily in an equal amount. The both silages were included in TMR at 20% level (as fed basis). pH in the rumen fluid was not influenced by the diets but was slightly higher from TMR than from control. No difference was found in ammonia-N concentration between diets. Total VFA concentration was relatively increased in the cattle fed RS-TMR to the other diets up to 6h post feeding. The proportion of acetate was increased in the TMR feeding at right before feeding (0 h, p<0.005) and 9 h (p<0.048) post feeding compared with control. Propionate proportion was increased (p<0.046) in both TMRs while butyrate proportion was increased (p<0.029) at 1h post feeding compared to other diets. Effective degradability (ED) of DM and CP of RS-TMR was relatively increased to other diets, and EDNDF of both TMRs was higher than that of control diet due to the increased parameters b (p<0.039) and c (p<0.006) in TMR treatments. Whole track digestibility of most components in the TMRs was slightly increased compared to that in control diet, and RS-TMR had a tendency to be increased whole track digestibility except for NDF compared to BS-TMR. Based on the results observed from the present study, nutrient availability of whole crop silage based TMR looked slightly better than conventional separate feeding of concentrate and rice straw, mainly due to the improved stabilization of fermentation in the rumen and increased NDF digestibility of whole crop silage in TMR.

Differences in Thrombolytic Effects in Accordance with Dosing-regimens of Tissue-type Plasminogen Activator in Experimental Pulmonary Embolism (실험적 폐색전증에서 조직형플라스미노겐활성체의 투여방법에 따른 혈전용해효과의 차이)

  • Chung, Hee-Soon;Kim, Ho-Jung;Han, Yong-Chol
    • Tuberculosis and Respiratory Diseases
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    • v.40 no.2
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    • pp.123-134
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    • 1993
  • Background: Tissue-type plasminogen activator is a physiologic activator, which has high affinity for fibrin and is activated by fibrin. Because of these properties, t-PA has the potential to induce effective thrombolysis without producing a systemic lytic state. In practice, however, therapeutically efficacious doses of t-PA has been associated with the development of a systemic lytic state. As experience with t-PA has accumulated, it has suggested that the fibrin selectivity is influenced by the dose and duration of t-PA infusion, and many studies have performed in an attempt to optimize the duration of t-PA regimen. Methods: This study was designed to assess the thrombolytic efficacy of t-PA and the differences of two dosing regimens of t-PA (infusion of 1 mg/kg t-PA over 15 or 180 minutes) in a canine model of pulmonary embolism, induced by injection of radioactive autologous blood clots. By continuously counting over both lung fields with a external gamma counter, we correlated rate and extent of pulmonary thrombolysis with corresponding pulmonary hemodynamics in addition to the gas analyses of arterial and mixed venous blood. Results: 1) While total clot lysis was similar ($36.2{\pm}3.3%$ and $39.6{\pm}2.3%$ respectively, p>0.05) when t-PA was infused over 15 or 180 minutes, the rate of lysis during infusion was markedly increased with the shorter infusion ($81.4{\pm}16.8%/hr$ vs $37.3{\pm}2.4%/hr$, p<0.05). 2) The duration of thrombolysis was $63.3{\pm}22.2$ minutes although t-PA was administered over 15 minutes, and it was only $148.5{\pm}14.0$ minutes in case of the infusion over 180 minutes (p<0.05). 3) The increased rate of thrombolysis with the shorter infusion was accompanied by a faster amelioration of cardiopulmonary impairment from pulmonary embolism (p<0.05). Conclusion: It is concluded that the shorter (15 minutes) infusion of t-PA is superior to the longer (180 minutes) infusion when the dose is equal, in consideration of the faster improvement in cardiopulmonary impairment from pulmonary embolism.

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Effect of N Application Level on Herbage Yield and Quality in Kentucky Bluegrass Pastures for Turf of School Ground (학교운동장용 Kentucky bluegrass 초지의 N 시비수준이 건물수량과 품질에 미치는 영향)

  • Lee, In-Duk;Lee, Hyung-Suk
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.25 no.3
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    • pp.199-204
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    • 2005
  • The object of this experiment was to suggest the optimum N fertilization level in Kentucky bluegrass pasture fer turf'of school ground. The field trials were conducted from 2001 to 2003 at Chungnam National University in order to evaluate the dry matter yield and quality on the N levels(100, 150 and 200kg/ha). With increasing the N level, the average DM yield for 2 years in Kentucky bluegrass pasture significantly increased. The average total DM yields was 6,317, 8,495 and 10,361 kg/ha at N 100, 150 and 200 kg/ha level(p<0.05), respectively. CP content and DMD were significantly increased(p<0.05), whereas fibrous constituents were not detected obvious tendency, but CPDM and DDM yields were significantly increased with increasing the N levels for 2 years(p<0.05). Based on the results, considering the total DM yield and quality of herbages which incidentally produced from Kentucky bluegrass pasture in school ground, it can be suggested that the suitable application N level was 200 kg/ha.

대추의 성숙중에 일어나는 연화현상에 관한 연구

  • 손미애;김광수
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 1994.07a
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    • pp.23-24
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    • 1994
  • 본 연구는 성숙과의 저장중에 세포벽분해효소가 세포벽을 분해해서 물성의 변화를 유발함으로 일어나는 과실의 연과가 품질과 저장성의 저하 뿐만 아니라 영양적, 경제적 손실을 초래한다는 점을 고려하여 대추의 성숙중에 일어나는 연화현상을 연구하고자 성숙에 따른 경도, 세포벽분해효소의 활성, 세포벽 다당류 pectin질, 비섬유성 중성당 및 조직의 변화를 조사하였다. 경도는 대추의 숙성에 따라 감소하였고, polygalacturonase와 $\beta$-galactosidase의 활성은 각각 변색기와 완숙기에 나타난 이후 급격히 증가하였다. 세포벽 다당류인 pectin질과 알칼리 지용성 hemicellulose는 완숙기가지 증가햐였으나 cellulose는 완숙기에 산가용성 hemicellulose와 cellulose를 제외한 세포벽 다당류의 함량은 다소 감소하였다. 대추의 세포벽 비섬유성 중성당으로 rhamnose, arabinose, xylose, mannose, galactose, glucose가 동정되었고, 성숙동안에 pectin질에서는 arabinose, mannose, galactose와 총 비섬유성 중성당의 함량이 감소하였고, 산가용성 hemicellulose에서는 xylose와 mannose가 뚜렸하게 증가하였으나 중성당은 변화없었으며, 알칼리 가용성 hemicellulose에서는 성숙에 따른 변화가 거의 없었다. pectin질의 경우 수용성 pectin, EDTA 용해성 pectin 및 총 pectin은 성숙중에 증가하는 경향이있으나 불용성 pectin은 감소하는 경향이였으며 과숙기에는 불용성 psctin EDTA용해성 pectin 및 총 pectin의 함량은 모두 현저히 감소하였다. 대추의 성숙중 조직에서는 pectin질로 구성된 중충의 붕괴현상이 뚜렸하게 나타났다.발이 절실히 필요한 실정이다. 이러한 배경으로 본 강연에서는 효소적갈변 저해제의 개발과 그들의 식품가공에의 적용 현환 및 화장품, 의약품으로의 응용에 대해 설명하고자 한다.L주에 비해 S주는 수정후 용과가 더 심하다. 9) 화분관의 행동은 수정력과 완전히 일치된다. 즉 L-selfing, $L{\times}L$, S-selfing, $S{\times}S$등의 부적법 수분에서는 화분관은 화주의 미중에서 정지되지만 $L{\times}S$, $S{\times}L$,에서는 수분 약 40-50분 후이면 화분관은 자방까지 도달된다. 10) S주는 웅본으로 오인되어 있지만 인위적법수분을 하면 수정력이나 화분관의 행동은 L주에서와 동일하다. 11) S화분은 완전하지만 L화분은 약 70%가 내용공허한 Adortive pollen 이다. 12) L화분중 나머지 30%도 S화분에 비해서 염색도가 낮은것이 많고 S화분 같이 농염되는 것은 극히 소수이다. 13) 본장물은 분화가 고도로 진행된 전형적인 이형예작물이여 마치 Dimorphism 에서 Dioecious 에로 이행되는 수가 있다는 것을 표시하는 증거가 되는 것 같다. 다소 높은 산소농도 3%~5% 이산화탄소 농도 5~8%에서 저장하는 것이 효과적일 것으로 판단되었다.철쭉군목으로 대표되나 군단이 하의 군목들은 다소 차이를 보이는 것으로 나타났다. 중간상인이론의 수정이 필요하다고 본다.가\ulcorner 본 논문에서는 표면적 형태에도 불구하고 [-wh]의미의 겹의문사는 병렬적 관계의 합성어가 아니라 내부구조를 지니지 않은 단순한 단어(minimal $X^{0}$ elements)로 가정한다. 즉, [+wh] 의미의 겹의문사는 동일한 구성요 소를 지닌 병렬적 합성어([

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The Chemical Composition and Ruminal Dry Matter Digestibility of Leaves+Stems, Leaves, Stems and Roots of Aralia cordata Thunberg as a Roughage Sources (조사료원으로서 땅두릅(Aralia cordata Thunberg) 잎+줄기, 잎, 줄기 및 뿌리의 화학적 조성 및 반추위내 건물소화율)

  • Kim, Yong Ik;Lee, Hyung Suk;Kim, Yong Kook
    • Korean Journal of Agricultural Science
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    • v.26 no.1
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    • pp.58-64
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    • 1999
  • The chemical composition and ruminal dry matter digestibilities of leaves, stems and roots of Aralia cordata Thunberg were determined and compared each other as a roughage sources for ruminants. The crude protein contents were higher for leaves(12.4%) than for leaves+stems (9.7%), stem(5.1%) and roots (3.8%) (P<0.05). The crude fat contents were higher for leaves (3.7%) than for roots (2.1%) and stems (1.3%) (P<0.05). The crude fiber contents were lower for roots (12.3%) than for leaves (15.0%), leaves+stems (27.7%) and stems (40.3%) (P<0.05), respectively. The contents of neutral detergent fiber were lower for leaves (30.2%) than for leaves+stems (42.0%), roots (50.8%) and stems (60.0%) (P<0.05), respectively. The contents of acid detergent fiber were lower for root(18.3%) than for leaves(21.4%). leaves+stems (37.5%) and stems (49.6%) (P<0.05), respectively. The calcium content of leaves(2.4%) were higher than those of stems and roots (0.97% and 0.69%), however the phosphorus contents were similar among leaves, stems and roots(0.25%, 0.19% and 0.35%). Ruminal dry matter digestibilities for 12, 24, 48 and 72hr of leaves(38.9%, 65.9%, 79.8% and 82.4%) and roots(38.9%, 59.8%, 77.6% and 78.5%) were higher than stems(31.1%, 44.1%, 49.5% and 52.6%). Furthermore the digestibilities of leaves were higher than those of alfalfa hay(37.4%, 48.8%, 67.8% and 71.8%) and although the digestibilities of stems which were the lowest among the parts were higher than those of acasia wood chip(12.6%, 18.2%, 21.6% and 24.3%).

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Effects of Dried Whole Crop Barley Treated with Cellulolytic Microorganisms on In Vitro Fermentation Characteristics in Swine (섬유소 분해균을 이용한 건조 청보리 발효사료가 돼지의 In vitro 발효 특성에 미치는 영향)

  • Park, Do-Yeun;Park, Joong-Kook;Cho, Sung-Back;Kim, Chang-Hyun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.30 no.2
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    • pp.179-190
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    • 2010
  • The experiment was conducted to observe the effects of dried whole crop barley treated with cellulolytic microorganisms (Aspergillus niger KCCM 60357 and Bacillus licheniformis KCCM 40934) on the chemical composition, in vitro colonic fermentation and whole tract digestibility in swine. Whole crop barley were fermented with no microorganism addition (control), A. niger, B. licheniformis and co-culture of A. niger and B. licheniformis (Mixture) for 3 days at $30^{\circ}C$. In the feed chemical composition, CP contents of whole crop barley treated with A. niger (7.52%) and B. licheniformis (7.77%) were significantly higher than control (6.81%) (p<0.05). The in vitro colonic fermentation of dried whole crop barley fermented with control showed significantly higher $CH_4$ contents than A. niger, B. licheniformis and Mixture at 18h incubation (p<0.05). Dry matter (DM) digestibilities of A. niger (55%) and Mixture (57.42%) treatments were significantly higher than control (43.74%) (p<0.05). Ammonia-N was significantly increased in A. niger, B. licheniformis and Mixture relative to control at 24 hour incubation (p<0.05). Xylanase activities in A. niger, B. licheniformis and Mixture treatments were significantly higher than control at 24 hour incubation (p<0.05). Concentrations of total VFA were significantly increased in B. licheniformis (12.61 mM) at 24hour incubation (p<0.05). In vitro whole tract digestibility was significantly increased in B. licheniformis (49.61%) compared with the control (45.65%) (p<0.05). In conclusion, whole crop barley treated with cellulolytic microorganisms improved whole tract digestibility and colonic fermentation for swine.

Distribution of Vascular Plants and Plant Resources Characteristics in Hapcheon-gun, Gyeongsangnamdo Province - Mt. Odo, Mt. Hwangmae, Mt. Daeam, and Muweol Peak - (경남 합천군의 관속식물 분포와 자원특성에 관한 연구 - 오도산, 황매산, 대암산, 무월봉을 중심으로 -)

  • Han, Jong-Won;Kim, Hyun-Jun;Kang, Shin-Ho;Yang, Sun-Gyu;Park, Jeong-Mi;Jang, Chang-Gee
    • Korean Journal of Environment and Ecology
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    • v.24 no.4
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    • pp.406-425
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    • 2010
  • The flora for resource plants in Hapcheon-gun were investigated 10 times from March to October, 2009. Based on collected voucher specimens, the flora of this area consisted of 489 taxa in total; 82 families, 292 genera, 427 species, 4 subspecies, 49 varieties and 9 forms. The resources plants in the area were categorized by usage into 9 groups including an unknown group: 187 edible, 126 pasturing, 137 medicinal, 112 dyeing, 52 ornamental, 16 timber, 9 fiber, 2 industrial taxa and 134 unknown resource plants were recorded respectively. In addition, there were 18 taxa of Korean endemic plants, 33 taxa of specific plants which were designated by the Ministry of Environment, and 2 taxa of rare plants. Furthermore, 44 taxa of naturalized plants were observed in this investigated area. The vegetation status of the investigated area was well conserved, but it is likely that the contaminated area could be rapidly increased and exacerbated by the climbers and development of forest roads. Therefore, the systems for conservation need to be prepared for the effective management of forests, and there must be special rules to control the number of climbers and visitors.

Video-Assisted Thoracoscopic Surgery for Fibrinopurulent Empyema (섬유농성 농흉의 비디오 흉강경을 이용한 치료)

  • 손정환;모은경;지현근;김응중;신호승;신윤철
    • Journal of Chest Surgery
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    • v.36 no.6
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    • pp.404-410
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    • 2003
  • Different treatment options are available according to the stage and duration of the empyema. Stage I empyema (exudate stage) is treated concurrently by the administration of appropriate antibiotics and chest tube drainage. Stage III empyema (organized stage) is considered for decortication through an open thoracotomy. However, the treatment of fibrinopurulent, stage II empyema remains controversial. Recently, debridement with the use of Video-Assisted Thoracoscopic Surgery (VATS) has been proposed for the treatment of stage II empyema. We analyzed and report our initial experience of 5 cases of stage II empyema, treated with the use of VATS. Material and Method: Between June 2001 and February 2002, 5 patients with fibrinopurulent empyema that did not respond to antibiotics, chest tube drainage or Percutaneous Catheter drainage (PCD), and instillation of fibrinolytic agent were treated by debridement and irrigation with the use of VATS. A CT scan was performed in all patients before the operation to confirm the diagnosis of loculated empyema and to detect additional lung parenchymal diseases. Result: All 5 patients underwent successful debridement and irrigation with the use of VATS and the chest tube was inserted properly. And no patients needed conversion to open thoracotomy. The ratio of sex was 4 : 1 (male : female), the mean age was 53 years old (range, 26~73 years), the mean operative time was 73.4 minutes (range, 52~95 minutes), the mean duration of postoperative chest tube placement was 12.4 days (range, 6~19 days), and the mean duration of postoperative hospital stay was 20.8 days (range, 10~36 days). In all patients, clinical symptoms such as pain and fever subsided and simple chest PA view revealed satisfactory lung expansion. No major postoperative complication was observed during the hospital course and no patient suffered from the recurrence of empyema in the follow-up period. Conclusion: We think that early operation with the use of VATS is safe and efficient for stage II empyema which did not respond to medical treatment(antibiotics and chest tube drainage), therefore, it can prevent stage II empyema from advancing to stage III, organized empyema.

백상지 공정의 폐쇄화에 따른 초지계내 전분 축적현상에 대한 시뮬레이션 연구

  • 이학래;안현견
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 2000.11a
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    • pp.138-138
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    • 2000
  • 국내의 백상지 공정은 공정에 투입되는 청수의 양을 줄이면서 동시에 폐수 배출 양을 감 소시키기 위해 많은 노력을 하고 있다. 공정으로 유입되는 청수의 양과 처리된 폐수의 양을 줄이기 위한 방법으로 PDFCpolydisk filter)를 도입하여 백수를 여과하여 showertf sealing 에 재사용하고 있으며 공정수 재활용에 따른 유기 물질과 무기물질의 계 내로의 축적을 방 지하기 위한 효과적인 폐수 처리방법을 모색하고 있다. 일반적으로 청수를 백수로 대체할 경우 공정 백수 내에 TDSCtotal dissolved solid), T TSSCtotal suspended solid), CODCchemical oxygen demand), 전기 전도도와 칼숨 경 도 등이 증가되며 음이온성 저해 물질Canionic trash)이 증가하여 보류 및 지력증강용 첨가제의 효율 을 떨어뜨릴 뿐만 아니라 마모, 슬라엄, 펠트 막힘 등의 문제를 유발하게 된다고 알려져 있다. 청수를 백수로 대체함에 따라 생기는 이러한 문제를 해결하면서 효율적인 청수 절약 방안 을 세우기 위해서는 무엇보다도 문제를 유발하는 원인 물질의 축적 양을 예측하는 것이 중 요하다고 판단된다. 본 연구는 백상지 공정의 폐쇄화 수준이 높아짐에 따른 공정 백수 내의 유기물질의 축적 현상을 분석하는 것올 목적으로 하였다. 이를 위해 산화전분을 유기물질의 대표하는 물질로 설정하였다. 이는 백상파지와 함께 초지계 내로 유업되는 산화전분은 파지의 4%를 차지할 정도로 유입량이 많을 뿐만 아니라 음이온성을 띄고 있어서 지료에 홉착율이 낮고 양이온성 고분자의 효율을 저해하며 슬라임의 원인이 되기도 하는 물질이기 때문이다. 산화전분의 축적 현상을 분석하기 위하여 pilot 설비 상세 설계도를 참고하여 하루 생산량 이 16 T/D이고 백상파지만이 파지로 유입되는 백상지 생산 공정을 모델로하였으며, 산화전 분의 홉착과 용출 모델을 만들어 상용 시율레이터를 이용하여 시율레이션 프로그램을 작성 하였다. 시률레이션 프로그램에서는 장섬유 미세섬유, 충전제를 지료 구성 성분으로 설정하였고 O Orccotoma 등이 사용한 일과 보류도 모델을 응용하여 보류도 모델올 만들었다. 산화전분은 백상파지에 포함된 형태로만 초지계 내로 유입되며 백상파지가 해리되는 과정에서 완전히 백수에 용출되었다가 지료 구성 성분에 홉착되는 것으로 가정하였다. 지료 홉착된 산화전분 의 양은 용존 산화전분 총량에 비례하는 것으로 가정하였으며, 이 때 이 비례상수를 전분 홉착율이라 정의하였다. 시율레이션 결과, 공정 폐쇄화가 진행됨에 따라 백수 내의 산화 전분 농도는 증가하게 되 며, 폐쇄화 수준이 높아질수록 백수 내 전분 농도의 증가량은 더 높아졌다. 백수 내의 전분 농도의 증가량은 백상파지 첨가량이 증가할수록, 표면 사이징 양이 증가할수록 커졌다.

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