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An Evaluation of Plant Growth Promoting Activities and Salt Tolerance of Rhizobacteria Isolated from Plants Native to Coastal Sand Dunes (해안사구의 토착식물로부터 분리된 근권세균의 내염능과 식물성장촉진능 평가)

  • Hong, Sun Hwa;Lee, Mi Hyang;Kim, Ji Seul;Lee, Eun Young
    • Microbiology and Biotechnology Letters
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    • v.40 no.3
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    • pp.261-267
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    • 2012
  • Coastal sand dunes are important for ecosystems due to the variety of rare species that can be found in this kind of habitat, and the beautiful landscapes they create. For environmental remediation, a potential strategy is phytoremediation using the symbiotic relationship of plants and microbes in the rhizosphere, which has proven ecologically sound, safe, and cost effective. Ninety-five colonies were isolated from the rhizosphere soil (RS) or rhizoplane (RP) of Rorippa islandica, Rumex crispus, Artemisia princeps var. orientalis, Lilium sp Stellaria media, and Gramineae. These colonies were then tested for plant growth promoting activities (PGPAs) such as 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, and siderphores synthesis ability. In addition, salt tolerance was evaluated at 4% and 8% salt concentrations. It was observed that amongst the test subjects about 50% of the strains had a high resistance to salinity. Many of them could produce indole-3-acetic acid (IAA) IAA (in RS 13.9% and in RP 7.6%), exhibited ACC deaminase activity (55.8% in RS and 36.6% in RP), and could synthesize siderphores (62.7% in RS and 50% in RP). Correlation coefficient analyses were carried out for the three kinds of plant growth promoting abilities (PGPA) and salt tolerance. A positive correlation was found between an ability to synthesize siderphores and ACC deaminase activity (r=0.605, p<0.037). Similarly, positive correlations were noted between salt tolerance and ACC deaminase activity (r=0.762, p<0.004, r=0.771), and salt tolerance and an ability to synthesize siderphores (r=0.771, p<0.003).

Plant Growth-promoting Ability by the Newly Isolated Bacterium Bacillus aerius MH1RS1 from Indigenous Plant in Sand Dune (해안 사구에서 서식하는 토착식물로부터 분리된 근권미생물 Bacillus aerius MH1RS1의 식물성장 촉진 능력 연구)

  • Lee, Eun Young;Hong, Sun Hwa
    • Journal of Korean Society of Environmental Engineers
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    • v.35 no.10
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    • pp.687-693
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    • 2013
  • Coastal sand dunes have been seriously damaged caused by the development thoughtless for the environment and coastal erosion and destruction due to artificial structures like coast roads and breakwater. Hereupon, in this study we made a library of rhizobacteria that have the plant growth-promoting ability for plant rhizosphere of indigenous plants inhabiting in a coastal sand dune as well as the strong tolerance to salt, and evaluated the plant growth-promoting ability of these strains. Furthermore, we evaluated the effect of rhizobacteria on the growth rate of saline tolerant plants in sandy soil; selected out the most useful micro-organism for the restoration of a damaged sand dune. The effect of inoculation of strains selected from the first experiment on the growth of Peucedanum japonicum and Arundo donaxes planted in a coastal sand dune was evaluated. As a result, Bacillus aerius MH1RS1 had plant growth promoting activities: indole acetic acid (IAA) production, siderophores and 1-aminocyclopropane-1-carboxylate deaminase (ACC deaminase) activity, and also had a salinity tolerance. Also, in case of Peucedanum japonicum, the length of stems and weights of roots were enhanced by the inoculation of B. aerius MH1RS1. Fresh weights of stems and weights of roots in experimental group were, in particular, increased by 25% comparing with the control group. For an Arundo donax in experimental group, plant length increased by 18%, and weight of roots by 20% which is significant.

The Trend in Household Catastrophic Medical Expenditure according to Healthcare Coverage Types and Its Associated Factors (의료보장 형태에 따른 연간 가구 과부담 의료비 지출 추이와 관련요인)

  • Lee, Seon Hwa;Kam, Sin;Lee, Won Kee
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.6
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    • pp.4067-4076
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    • 2015
  • This study aims to examine the trend in household catastrophic medical expenditure according to the healthcare coverage types and its associated factors based on the raw data of the Korean Health Panel over the years 2008 to 2011. Correspondence analysis was used to investigate the trend in the incidence rates of annual catastrophic medical expenditure and generalized estimating equation to examine the factors influencing the incidence of catastrophic medical expenditure. The annual mean incidence rates of household catastrophic medical expenditure were 25.1%, 15.4%, 10.1%, 5.4% and 3.2% in the threshold levels of 10%, 15%, 20%, 30%, and 40% respectively. The incidence rate of household catastrophic medical expenditure was higher when the total annual household income was lower, the education level of the householder was lower, the healthcare coverage type was National Health Insurance, the householder had disability, the age of the householder was older, the number of household members was smaller, the subjective health status of household members was lower, and the prevalence rate of the chronic disease of the household was higher(p<0.05). Therefore, a policy for vulnerable households with older or patient members of chronic diseases should be established.

Monitoring Anaerobic Reductive Dechlorination of TCE by Biofilm-Type Culture in Continuous-Flow System (연속흐름반응조에서 바이오필름형태의 탈염소화 미생물에 의한 TCE분해 모니터링)

  • Park, Sunhwa;Han, Kyungjin;Hong, Uijeon;Ahn, Hongil;Kim, Namhee;Kim, Hyunkoo;Kim, Taeseung;Kim, Young
    • Journal of Soil and Groundwater Environment
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    • v.17 no.5
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    • pp.49-55
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    • 2012
  • A 1.28 L-batch reactor and continuous-flow stirred tank reactor (CFSTR) fed with formate and trichloroethene (TCE) were operated for 120 days and 56 days, respectively, to study the effect of formate as electron donor on anaerobic reductive dechlorination (ARD) of TCE to cis-1,2-dichloroethylene (c-DCE), vinyl chloride (VC), and ethylene (ETH). In batch reactor, injected 60 ${\mu}mol$ TCE was completely degraded in the presence of 20% hydrogen gas ($H_2$) in less than 8 days by anaerobic dechlorination mixed-culture (300 mg-soluble protein), Evanite Culture with ability to completely degrade tetrachloroethene (PCE) and -TCE to ETH under anaerobic conditions. Once the formate was used as electron donor instead of hydrogen gas in batch or chemostat system, the TCE-dechlorination rate decreased and acetate production rate increased. It indicates that the concentration of hydrogen produced in both systems is possibly more close to threshold for homoacetogenesis process. Soluble protein concentration of Evanite culture during the batch test increased from 300 mg to 688 mg for 120 days. Through the protein monitoring, we confirmed an increase of microbial population during the reactor operation. In CFSTR test, TCE was fed continuously at 9.9 ppm (75.38 ${\mu}mol/L$) and the influent formate feed concentration increased stepwise from 1.3 mmol/L to 14.3 mmol/L. Injected TCE was accumulated at 18 days of HRT, but TCE was completely degraded at 36 days of HRT without accumulation of the injected-TCE during the left of experiment period, getting $H_2$ from fermentative hydrogen production of injected formate. Although c-DCE was also accumulated for 23 days after beginning of CFSTR operation, it reached steady-state in the presence of excessive formate. We also evaluated microbial dynamic of the culture at different chemical state in the reactor by DGGE (denaturing gradient gel electrophoresis).

Wide Bandgap 박막 태양전지 제작을 위한 P-type a-$SiO_x$:H layer 최적화에 관한 연구

  • Yun, Gi-Chan;Kim, Yeong-Guk;Park, Seung-Man;Park, Jin-Ju;Lee, Seon-Hwa;An, Si-Hyeon;Lee, Jun-Sin
    • Proceedings of the Korean Vacuum Society Conference
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    • 2010.02a
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    • pp.153-153
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    • 2010
  • p-i-n 형 비정질 실리콘 박막 태양전지에서 p층은 창물질(window material)로서 전기 전도도가 크고, 빛 흡수가 적어야한다. p층의 두께가 얇으면 p층 전체가 depletion layer가 되고 충분한 diffusion potential을 얻을 수 없어 open-circuit voltage ($V_{oc}$)가 작아진다. 반대로 p층 두께가 두꺼워지면 빛 흡수가 증가하고, 표면 재결합이 문제가 되어 변환효율이 감소한다. 밴드갭이 큰 물질로 창층을 제작하게 되면 보다 짧은 파장의 입사광이 직접 i층을 비추므로 Short-circuit current ($I_{sc}$) 와 fill factor를 증가시킬 수 있다. 하여 본 연구에서는 기존의 창층으로 사용되는 Boron을 doping한 p-type a-Si:H 대신에 $N_2O$를 첨가한 p-type a-$SiO_x$:H의 $N_2O$ flow rate에 따른 밴드갭의 변화에 관한 연구를 수행하였다. p-type a-$SiO_x$:H Layer는 $SiH_4$, $H_2$, $N_2O$, $B_2H_6$ 가스를 혼합하여 증착하게 되는데 $SiH_4$, 가스와 $H_2$ 가스의 혼합비는 1:20, $B_2H_6$ 농도는 0.5%로 고정 하였으며 $N_2O$의 flow rate을 가변하며 증착하였다. $N_2O$의 가변조건은 5에서 50sccm으로 가변하여 증착하며 일반적으로 사용되는 RF-PECVD (13.56MHz)를 이용하였고 증착 온도는 175도, 전극간의 거리는 40mm, 파워와 압력은 30W, 700mTorr로 고정하여 진행하였다. 전기적 특성을 알아보기 위해 eagle 2000 Glass를 사용하였고 구조적 특성은 p-type wafer를 사용하여 각각 대략 200nm의 두께로 증착하였다. 증착 두께는 Ellipsometry를 이용하였으며 전기 전도도는 Agilent사의 4156c를 구조적특성은 FT-IR을 사용하여 측정하였다. Conductivity(${\sigma}_d$)는 $N_2O$가 증가함에 따라 $8.73\;{\times}\;10^{-6}$에서 $5.06\;{\times}\;10^{-7}$으로 감소하였고 optical bandgap ($E_{opt}$)은 1.71eV에서 2.0eV로 증가함을 알 수 있었다. 또한 reflective index(n)의 경우는 4.32에서 3.52로 감소함을 나타내었다. 기존의 p-type a-Si:H에 비해 상당한 $E_{opt}$을 가지므로 빛 흡수에 의한 손실을 줄임으로서 $V_oc$를 향상 시킬 수 있으며 동시에 짧은 파장에서의 입사광이 직접 i층을 비추므로 $I_{sc}$와 FF를 향상 시킬 수 있으리라 예상된다. 다소 낮은 전도도만 개선한다면 고효율의 박막 태양전지를 제작 할 수 있을 것으로 기대된다.

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Fundamentals of Ecotoxicity Evaluation Methods Using Domestic Aquatic Organisms in Korea : (II) Water Flea (국내 생물종을 이용한 생태독성평가 기반연구 : (II) 물벼룩류)

  • An, Youn-Joo;Nam, Sun-Hwa;Lee, Woo-Mi
    • Korean Journal of Ecology and Environment
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    • v.40 no.3
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    • pp.357-369
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    • 2007
  • Water fleas are very important organisms in aquatic ecosystem because they are key constituents of food webs as either glazers or foods for predators. Daphnia magna is a representative test species and it has been extensively used in ecotoxicity evaluation. However, Daphnia magna has not been found yet in Korean water environment. There are limitations of using the foreign species such as Daphnia magna to reflect domestic situations in Korea. Consequently it is mandatory to find domestic species suitable for bioassay, and to develop corresponding toxicity test methods using the domestic species. In this study, we widely collected the domestic and foreign toxicity test methods performed by using domestic water fleas in Korea. The 58 test methods were collected from the standard methods (OECD, US EPA, ASTM), government reports, SCI papers and domestic papers. Ten domestic water fleas selected were Bosmina longirostris, Ceriodaphnia dubia, Ceriodaphnia reticulata, Daphnia obtusa, Daphnia puex, Moina macrocopa, Moina micrura, Simocephalus mixtus, Simocephalus serrulatus, and Simocephalus vetulus. We suggested the domestic ecotoxicity test methods using domestic water fleas in Korea through providing a range of test conditions, and future directions for toxicity test using water flea. This study could be a useful basis for establishing the aquatic toxicity test methods with domestic organisms in Korea.

Expanding the Substances of Water Quality Standard for the Protection of Human Health Based on Risk Assessment (인체 위해성기반 수질환경기준 항목 확대를 위한 연구)

  • An, Youn-Joo;Nam, Sun-Hwa;Lee, Jae-Kwan
    • Korean Journal of Ecology and Environment
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    • v.41 no.1
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    • pp.34-42
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    • 2008
  • Water quality standards (WQS) are mandatory to guarantee the human health and protection of aquatic ecosystems, and maintain the condition of suitable water quality. The present WQS for the protection of human health in Korea contain nine substances (As, Cd, $Cr^{6+}$, CN, Pb, Hg, ABS, organophosphorus compounds and PCBs), but it is insufficient to preserve the human and aquatic ecosystem from a variety of chemicals. Therefore, it is necessary to expand the substance of WQS for the protection of human health. In this study, we chose the 20 chemicals from 43 chemicals of the project entitled 'Development of Integrated Methodology for Evaluation of Water Environment'. The methodology for calculating water quality criteria was amended from the US Environmental Protection Agency (US EPA)'s equation for deriving ambient water quality criteria for the protection of human health. The factors including fish intake, drinking water intake, and human body weight used in the equation reflected Korean situations. The monitoring values were derived from the water quality monitoring data in Korean four main rivers. The orders of priorities of chemicals were evaluated by human health risk assessment, and the proposed WQS was derived by technical and economic analyses. These results were reflected to expand the WQS for the protection of human health.

Effects of Changes of Nutrient Solution Concentration According to Growth Stage on Growth and Flowering of Cut Chrysanthemum Grown Hydroponically in Perlite (국화의 펄라이트 양액재배시 생육단계에 따른 양액농도의 변화가 생육과 개화에 미치는 영향)

  • Ji, Eun Young;Oh, Wook;Kim, Sun Hwa;Kim, Ki Sun
    • Horticultural Science & Technology
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    • v.16 no.2
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    • pp.247-250
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    • 1998
  • This study was carried out to investigate the effects of changes of ionic strength according to growth stage on growth and flowering of Dendranthema grandiflorum (Ramat.) Kitamura 'Seiun' grown hydroponically in perlite. The stage I, II, and III covered early vegetative growth (27-40 days after planting), latter vegetative growth (41-54 days), and reproductive growth (55-80 days), respectively. The 2 strength (1S and 2S) of nutrient solution were treated in stage I, whereas 3 strengths (1S, 1-2S, and 2S) were treated in stage II. Then, total 9 treatments in stage III were designated by 3 treatments (tap water, 1S, and 2S) for each 3 strengths in stage II. Each nutrient solution was applied 8 times per day. At vegetative growth stage (54 days after planting), stem length was highest when irrigated 8 times a day with 1S nutrient solution. Both photosynthesis and transpiration rate were higher in 1S than those in other treatments (1-2S, 2S), whereas leaf chlorophyll content was highest in 2S treatment. Ion content of plant treated with 2S was higher than other treatments. Growth (plant height, leaf area, stem length), fresh weight, and dry weight of each plant organ after flower bud formation were better in tap water treatment (1-1-0) than other 1S treatments (1-1-1, 1-1-2). Regarding the number of days to flowering, tap water treatment was the most effective. Thus, after flower bud formation supplying tap water or lower concentration of nutrient solution than those used during the vegetative growth stage was economical in saving chemical fertilizers, shortening the number of days to flowering, reducing salt accumulation in media, saving efforts of leaching, and reducing ground water contamination.

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Current Status of Hyperspectral Data Processing Techniques for Monitoring Coastal Waters (연안해역 모니터링을 위한 초분광영상 처리기법 현황)

  • Kim, Sun-Hwa;Yang, Chan-Su
    • Journal of the Korean Association of Geographic Information Studies
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    • v.18 no.1
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    • pp.48-63
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    • 2015
  • In this study, we introduce various hyperspectral data processing techniques for the monitoring of shallow and coastal waters to enlarge the application range and to improve the accuracy of the end results in Korea. Unlike land, more accurate atmospheric correction is needed in coastal region showing relatively low reflectance in visible wavelengths. Sun-glint which occurs due to a geometry of sun-sea surface-sensor is another issue for the data processing in the ocean application of hyperspectal imagery. After the preprocessing of the hyperspectral data, a semi-analytical algorithm based on a radiative transfer model and a spectral library can be used for bathymetry mapping in coastal area, type classification and status monitoring of benthos or substrate classification. In general, semi-analytical algorithms using spectral information obtained from hyperspectral imagey shows higher accuracy than an empirical method using multispectral data. The water depth and quality are constraint factors in the ocean application of optical data. Although a radiative transfer model suggests the theoretical limit of about 25m in depth for bathymetry and bottom classification, hyperspectral data have been used practically at depths of up to 10 m in shallow and coastal waters. It means we have to focus on the maximum depth of water and water quality conditions that affect the coastal applicability of hyperspectral data, and to define the spectral library of coastal waters to classify the types of benthos and substrates.

A Participatory Action Research of Social Workers on the Practice Methods for Single Parents Self-help Groups in Community Welfare Centers (지역사회복지관 한부모 자조집단 지원 실천 방안 모색을 위한 사회복지사 참여실행연구)

  • Yang, Haewon;Kim, Heesoo;Choi, Jung Sook;Kim, Sun Hwa;Kim, Eun Kyung
    • Korean Journal of Family Social Work
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    • no.61
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    • pp.93-136
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    • 2018
  • The purpose of this study is to find specific practice methods of single parents self-help groups in community welfare centers. A participatory action research was conducted to achieve the purpose. Six social workers and three researchers participated in the study who shared the problem of lack of specific practice methods even though a self-help group was useful for self-reliance and empowerment of low-income single-parents. Various activities such as reading articles, analysis of each self-help group, case studies, and learning facilitation methods were conducted during 10 meetings. At the same time, peer supervision proceeded at each meeting. The progress of each group was shared, the application of practice methods and plans were discussed, and applying discussed methods to group practice and reviewing the process and outcomes of the practice were repeated in the peer supervison. As a result, the following positive changes appeared: First, the goals and vision of each group became clearer. Second, initiative of the members was promoted. Third, intimacy, mutual cooperation, the attatchment to the group were strengthened. A qualitative analysis was also conducted on the content discussed. As a result, 17 practice methods were derived in four themes such as initiative/ empowerment, cohesion, leadership, and destandardization. Practice issues concerning single parents self-help groups in community welfare centers were discussed based on the results.