Browse > Article
http://dx.doi.org/10.4014/kjmb.1208.08003

An Evaluation of Plant Growth Promoting Activities and Salt Tolerance of Rhizobacteria Isolated from Plants Native to Coastal Sand Dunes  

Hong, Sun Hwa (Department of Environmental and Energy Engineering, The University of Suwon)
Lee, Mi Hyang (Department of Environmental and Energy Engineering, The University of Suwon)
Kim, Ji Seul (Department of Environmental and Energy Engineering, The University of Suwon)
Lee, Eun Young (Department of Environmental and Energy Engineering, The University of Suwon)
Publication Information
Microbiology and Biotechnology Letters / v.40, no.3, 2012 , pp. 261-267 More about this Journal
Abstract
Coastal sand dunes are important for ecosystems due to the variety of rare species that can be found in this kind of habitat, and the beautiful landscapes they create. For environmental remediation, a potential strategy is phytoremediation using the symbiotic relationship of plants and microbes in the rhizosphere, which has proven ecologically sound, safe, and cost effective. Ninety-five colonies were isolated from the rhizosphere soil (RS) or rhizoplane (RP) of Rorippa islandica, Rumex crispus, Artemisia princeps var. orientalis, Lilium sp Stellaria media, and Gramineae. These colonies were then tested for plant growth promoting activities (PGPAs) such as 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, and siderphores synthesis ability. In addition, salt tolerance was evaluated at 4% and 8% salt concentrations. It was observed that amongst the test subjects about 50% of the strains had a high resistance to salinity. Many of them could produce indole-3-acetic acid (IAA) IAA (in RS 13.9% and in RP 7.6%), exhibited ACC deaminase activity (55.8% in RS and 36.6% in RP), and could synthesize siderphores (62.7% in RS and 50% in RP). Correlation coefficient analyses were carried out for the three kinds of plant growth promoting abilities (PGPA) and salt tolerance. A positive correlation was found between an ability to synthesize siderphores and ACC deaminase activity (r=0.605, p<0.037). Similarly, positive correlations were noted between salt tolerance and ACC deaminase activity (r=0.762, p<0.004, r=0.771), and salt tolerance and an ability to synthesize siderphores (r=0.771, p<0.003).
Keywords
ACC deaminase; coastal sand dune; IAA; rhizobacteria; salt tolerance; siderphores;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Frankenberger, W. T. Jr, W. Brunner. 1983. Method of detection of auxin-indole-3-acetic acid in soil by high performance liquid chromatography. Soil Sci. Soc. Am. J. 47: 237-241.   DOI
2 Giovanelli, J., S. H. Mudd, and A. H. Datko. 1980. Sulfur amino acids in plants, pp. 453-505. In: Miflin, B. J. (ed.), Amino acids and derivatives. The biochemistry of plants: a comprehensive treatise. Vol. 5, Academic Press, New York, U.S.A.
3 Gutierrez Mafiero, E. J., N. Acero, J. A. Lucas, and A. Probanza. 1996. The infuence of native rhizobacteria on European alder (Alnus glutinosa (L.) Gaertn.) growth. Plant and Soil 182: 67-74.   DOI   ScienceOn
4 Hwang, J. S., Y. H. You, J. J. Bae, S. A. Khan, J. G. Kim, and Y. S. Choo. 2011. Effects of endophytic fungal secondary metabolites on the growth and physiological response of Carex kobomugi Ohwi. J. Coast Res. 27: 544-548.
5 John, P. 1991. How plant molecular biologists revealed a surprising relationship between two enzymes, which took an enzyme out of a membrane where it was not located, and put it into the soluble phase where it could be studied. Plant Mol. Biol. Rep. 9: 192-194.   DOI   ScienceOn
6 Johnson, D. L., D. R. Anderson, and S. P. McGrath. 2005. Soil microbial response during the phytoremediation of a PAH contaminated soil. Soil Biol. Biochem. 37: 2334-2336.   DOI   ScienceOn
7 Khan, S. A., M. Hamayun, H. J. Yoon. H. Y. Kim. S. J. Suh, S. K. Hwang, J. M. Kim, I. J. Lee, Y. S. Choo, U. H. Yoon, W. S. Kong, B. M. Lee, and J. G. Kim. 2008. Plant growth promotion and Penicillium citrinum. BMC Microbiol. 8: 231.   DOI   ScienceOn
8 Boopathy, R. 2004. Factors limiting bioremediation technologies( review paper). Bioresour. Technol. 74: 63-67.
9 Cattelan, A. J., P. G. Hartel, and J. J. Fuhrmann. 1999. Screening for plant growth-promoting rhizobacteria to promote early soybean grown. Soil Sci. Soc. Am. J. 63: 1670-1680.   DOI   ScienceOn
10 Deikman, J. 1997. Molecular mechanisms of ethylene regulation of gene transcription. Physiol. Plant 100: 561-566.   DOI   ScienceOn
11 Dell'Amico, E., L. Cavalca, and V. Andreoni. 2005. Analysis of rhizobacterial communities in perennial Graminaceae from polluted water meadow soil, and screening of metalresistant, potentially plant growth-promoting bacteria. FEMS Microbiol. Ecol. 52: 153-162.   DOI   ScienceOn
12 Dworkin, M. and J. W. Foster. 1958. Experiments with some microorganism which utilize ethane and hydrogen. J. Bacteriol. 75: 592-603.
13 Flannery, T. 2006. The history and future impact of climate change. pp. 131. The weather makers. Text publishing company PTY Ltd., Melbourne, Australia.
14 Koo, S. Y., and K. S. Cho. 2011. Characterization of Serratia sp. K1RP-49 for Application to the Rhizoremediation of Heavy Metals. Environ. Earth Sci. 1: 3-13
15 Khan, S. A., M. Hamayun, H. Y. Kim, H. J. Yoon, I. J. Lee, and J. G. Kim. 2009. Gibberellin production and plant growth promotion by a newly isolated strain of Gliomastix murorum. World J. Microbiol. Biotechnol. 25: 829-833.   DOI   ScienceOn
16 Khan, S. A., M. Hamayun, H. Y. Kim, H. J. Yoon, J. C. Seo, Y. S. Choo, I. J. Lee, S. D. Kim, I. K. Rhee, and J. G. Kim. 2009. A new strain of Arthrinium phaeospermum isolated from Carex kobomugi Ohwi is capable of gibberellins production. Biotechnol. Lett. 31: 283-287.   DOI   ScienceOn
17 Koo, S. Y., and K. S. Cho. 2006. Interaction between plants and rhizobacteria in phytoremediation of heavy metal-contaminated soil. Kor. J. Microbiol. Biotechnol. 2: 83-93.
18 Lee, M. S., J. O. Do, M. S. Park, S. Jung, K. H. Lee, K. S. Bae, S. J. Park, and S. B. Kim. 2006. Dominance of Lysobacter sp. in the rhizosphere of two coastal sand dune plant species, Calystegia soldanella and Elymus mollis. Antonie Leeuwenhoek 90: 19-70.   DOI   ScienceOn
19 Lim, J. H., Kim, J. G., and Kim, S. D. 2008. Selection of the auxin and ACC deaminase producing plant growth promoting rhizobacteria from the coastal sand dune plant. Kor. J. Microbiol. Biotechnol. 36: 268-275.
20 Martinez, M L., P. Moreno-Casasola, and G. Vazquez. 1997. Effects of disturbance by sand movement and inundation by water on tropical dune vegetation dynamics. Can. J. Bot. 75: 2005-2014.   DOI   ScienceOn
21 Maun, M. A. 1994. Adaptations enhancing survival and establishment of seedling on coastal dune systems. Vegetatio 111: 59-70.
22 Maun, M. A. and P. R. Baye. 1989. The ecology of Ammophila breviligulata Fern. On coastal dune ecosystem. CRC Cr. Rev. Aquat. Sci. 1: 661-681.
23 Reed, M. L. E., and B. R.Glick. 2005. Plant growth-promoting bacteria facilitate the growth of the common reed Phragmites australis in the presence of copper or polycyclic aromatic hydrocarbons. Curr. Microbiol. 51: 425-429.   DOI   ScienceOn
24 Nehl, D. B., S. J. Allen, and J. F. Brown. 1996. Deleterious rhizosphere bacteria an intergrating perspective(review). Appl. Soil Ecol. 5: 1-20.
25 Opelt, K. and G. Berg. 2004. Diversity and antagonistic potential of bateria associated with bryophytes from nutrientpoor habitats of Baltic sea coast. Appl. Environ. Microbiol. 70: 6569-6579.   DOI   ScienceOn
26 Poonguzhali, S., M. Madhaiyan, and T. Sa. 2006. Cultivation-dependent characterization of rhizobacterial communities from field grown Chiness cabbage Brassica campestris ssp pekinensis and screening of traits for potential plant growth promotion. Plant Soil 286: 167-180.   DOI   ScienceOn
27 Safronova, V. I., V. V. Stepanok, G. L. Engqvist, Y. V. Alekseyev, and A. A. Belimov. 2006. Rootassociated bacteria containing 1-aminocyclopropane-1-carboxylate deaminase improve growth and nutrient uptake by pea genotypes cultivated in cadmium supplemented soil. Biol. Fertil. Soils 42: 267-72.   DOI   ScienceOn
28 Schwyn, B. and J. B. Neilands. 1987. Universal chemical assay for the detection and determination of siderphores. Anal. Biochem. 160: 47-56.   DOI   ScienceOn
29 So, J. H., D. J. Kim, J. H. Shin, C. B. Yu, and I. K. Rhee. 2009. Isolation and characterization of Bacillus cereus A-139 producing auxin from east coast sand dunes. Kor. J. Environ. Agr. 28: 447-452.
30 Sylvia, D. M. 1989. Nursery inoculation of sea oats with vesicular-arbuscular mycorrhizal fungi and outplanting performance of Florida beaches. J. Coast. Res. 5: 747-754.
31 Sylvia, D. M., and M. E. Will. 1988. Establishment of vesicular-arbuscular mycorrhizal fungi and other microorganisms on a beach replenishment site in Florida. Appl. Environ. Microbiol. 54: 348-352.
32 Yang, S. F. and N. E. Hoffman. 1984. Ethylene biosynthesis and its regulation in higher plants. Annu. Rev. Plant Physiol. 35: 155-89.   DOI   ScienceOn