• Title/Summary/Keyword: 생화학적 특성

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Effects of Nutrition Education in Type 2 Diabetes Mellitus on Diabetes Control and Blood Antioxidant Status (제2형 당뇨환자에 대한 영양교육이 당뇨병 관리와 혈액 항산화 상태에 미치는 영향)

  • Shin, Kyung-Nam;Lee, Hye-Sang;Kwon, Chong-Suk
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.5
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    • pp.689-695
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    • 2011
  • Diabetic patients need nutritional education more than those suffering from other diseases because of the necessity of controlling blood glucose levels with dietary treatment. The purpose of this study was to find out the effectiveness of nutrition education on diabetes control and antioxidant status, both of which are related to diabetic complications. Thirty (15 males and 15 females) type 2 diabetes mellitus patients aged $66.7{\pm}8.8$ years participated in a 4-week nutrition education program. Nutrient intakes, blood glucose level, antioxidant status, and DNA damage were evaluated before, immediately after, and three months after the education program. Changes in those parameters over time were analyzed using repeated-measures analysis of covariance. Over time, HbA1c (p=0.000), plasma total cholesterol (p=0.002), plasma thiobarbituric acid related substances (TBARS; p=0.000), and leukocyte DNA damage (p=0.000) significantly decreased; plasma retinol (p=0.001), plasma tocopherol (p=0.000), erythrocyte catalase (CAT; p=0.000), and erythrocyte glutathione peroxidase (GPx; p=0.000) significantly increased. In an evaluation of nutrient intakes by Dietary Reference Intakes for Koreans (KDRI), energy (p=0.009), phosphorus (p=0.033), sodium (p=0.001), potassium (p=0.019), zinc (p=0.043), riboflavin (p=0.050), folic acid (p=0.048) and vitamin C (p=0.008) intakes had significant positive changes. In a correlation analysis of the biochemical and nutritional changes resulting from the education program, plasma TBARS were negatively correlated with potassium (r=-0.418, p<0.05), iron (r=-0.443, p<0.05), riboflavin (r=-0.432, p<0.05), and folic acid (r=-0.446, p<0.05) intakes, while plasma retinol was positively correlated with energy (r=0.543, p<0.01), protein (r=0.543, p<0.01), phosphorus (r=0.425, p<0.05), iron (r=0.485, p<0.05), zinc (r=0.570, p<0.01) and niacin (r=0.510, p<0.05) intakes. Erythrocyte CAT was positively correlated with folic acid intake (r=0.605, p<0.01). From these results, we suggest that an improvement in nutrition resulting from a diabetic education program for type 2 diabetes patients led to improvement in their antioxidant status, also possibly reducing complications resulting from diabetes.

Selection of Virulent Isolates of Fusarium circinatum and Investigation of Pitch Canker Severity of Pinus rigida and P. rigida × P. taeda Seed Orchards in Jeju Island (제주도(濟州道) 리기다 및 리기테다 채종원(採種園)에서 푸사리움가지마름병 피해도(被害度) 조사(調査) 및 병원성(病原性) 균주선발(菌株選拔))

  • Woo, Kwan-Soo;Kim, Young-Joung;Kim, Tae-Su;Lee, Seong-Kyu
    • Journal of Korean Society of Forest Science
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    • v.94 no.6
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    • pp.402-409
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    • 2005
  • This study was undertaken to compare and estimate the severity of pitch canker of individual trees of Pinus rigida and Pinus rigida ${\times}$ P. taeda in two seed orchards in Jeju island, in which the orchards have been damaged by the pitch canker for seven years. Wind-pollinated two-year-old seedlings of P. rigida and P. rigida ${\times}$ P. taeda, in which the seedlings of P. rigida ${\times}$ P. taeda were from seeds of phenotypically selected, uninfected(but untested) trees, were inoculated with the pathogenic fungus, Fusarium circinatum, isolated from P. rigida and P. thunbergii. The virulence of the isolates was also identified. Statistically significant difference was found in 'stem cankers'(SC; ${\chi}^2=7.76$, P=0.05) among 4 plantations of P. rigida ${\times}$ P. taeda of two seed orchards. P. rigida was higher in 'top kill' (TK) and 'branch tip symptoms' (BT) than those of P. rigida ${\times}$ P. taeda. In artificial inoculation tests, mortality of the seedlings from the resistant candidates was 14% higher than that of the seedlings from the susceptible candidates. This result may becaused by unknown pollen trees and/or candidate tree selection based only on phenotype. Two of five fungal isolates, C-6-L(9) and C-6-L(19), showed significantly higher mortality (68% and 60%, respectively) than others, suggesting that these isolates can be used as virulent isolates for a mass artificial inoculation. Resistance candidate seedlings that were selected from this study can be utilized as useful materials for fundamental studies of genetics and biochemistry to breed resistance varieties to pitch canker.

Prevalence of Metabolic Syndrome and Related Risk Factors of Elderly Residents in Andong Rural Area 2. Based on the Biochemical Measurements and Nutrient Intakes (안동 농촌지역 중년 및 노인 주민의 대사증후군 유병율과 관련 위험요인 분석 2. 생화학 측정결과와 영양소 섭취를 중심으로)

  • Lee, Hye-Sang;Kwon, Chong-Suk
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.10
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    • pp.1459-1466
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    • 2010
  • This study was performed to identify the association between the metabolic syndrome and the biochemical measurements and nutrient intakes. A total of 1,431 people (533 males, 898 females) aged over 45 years living in Andong rural area participated in this study in 2003. Plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT), $\gamma$-glutamyl transferase ($\gamma$-GT) and thiobarbituric acid reactive substances (TBARS) levels in metabolic syndrome were significantly higher than those in normal group. In multiple logistic regression, those biochemical measurements were found to be positively associated with the metabolic syndrome as the adjusted odds ratios (OR) 1.839 (p<0.001) by AST, 2.302 (p<0.01) by ALT, 2.143 (p<0.001) by $\gamma$-GT, and 1.874 (p<0.001) by TBARS. We also found that the increased level of those measurements tended to be strongly associated with high triglyceride among the metabolic syndrome components. However, the nutrient intakes between the metabolic syndrome and the normal group were not significantly different. Also, we could not find any nutrient intakes significantly associated with the metabolic syndrome, except high carbohydrate intake (>70% of kcal) compared to normal intake (55~70% of kcal) showed OR 0.781 (p<0.05). In analyzing the association of nutrient intakes with metabolic syndrome components, we found that the calorie intake was negatively associated with abdominal obesity (OR 0.696, p<0.05) and high fat intake (>25% of kcal) was positively associated with low HDL-cholesterol (OR 1.864, p<0.05). This study revealed that the biochemical measurements, such as plasma AST, ALT, $\gamma$-GT, and TBARS, are associated with metabolic syndrome, but considering the nutrient intakes, we suggest that further studies are needed to identify the associations.

Isolation and Identification of Agarose-degrading Bacterium, Pseudoalteromonas sp. GNUM08122 (아가로오스 분해세균인 Pseudoalteromonas sp. GNUM08122 분리 및 동정)

  • Kim, Yu-Na;Jeong, Yeon-Kyu;Kim, Mu-Chan;Kim, Sung-Bae;Chang, Yong-Keun;Chi, Won-Jae;Hong, Soon-Kwang;Kim, Chang-Joon
    • Microbiology and Biotechnology Letters
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    • v.40 no.1
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    • pp.1-9
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    • 2012
  • This study's aim was to isolate microorganisms producing agarase with a high activity, with possible applications in improving the performance of the pretreatment processes for bioethanol production. Marine algaes were collected from the south coast of Korea, from which three kinds of microorganisms were isolated. After a 4-day culture of these strains at $25^{\circ}C$, crude enzymes were obtained from culture supernatant or cell-free extract by ammonium sulfate precipitation and membrane dialysis. Agarase activity was observed in these crude enzymes. Notably higher specific activity was observed in the crude enzyme obtained from the culture supernatant rather than that from the cell-free extract. This indicates that a secreted enzyme has a much greater activity than a cellular enzyme. Crude enzymes from the GNUM08122 strain were inferred to have ${\alpha}$-agarase activity because release of p-nitrophenol was observed, possibly due to the cleavage of p-nitrophenyl-${\alpha}$-D-galactopyranoside. The 16S rRNA sequence of GNUM08122 showed a close relationship to Pseudoalteromonas issachenkonii KMM 3549 (99.8%) and Pseudoalteromonas tetraodonis IMA 14160 (99.7%), which led us to assign it to the genus Pseudoalteromonas. Biochemical and physiological study revealed that this strain can grow well at $40^{\circ}C$ under a wide range of pH (pH 4~8) in high-salt conditions (10% NaCl).

Utility-Based Video Adaptation in MPEG-21 for Universal Multimedia Access (UMA를 위한 유틸리티 기반 MPEG-21 비디오 적응)

  • 김재곤;김형명;강경옥;김진웅
    • Journal of Broadcast Engineering
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    • v.8 no.4
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    • pp.325-338
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    • 2003
  • Video adaptation in response to dynamic resource conditions and user preferences is required as a key technology to enable universal multimedia access (UMA) through heterogeneous networks by a multitude of devices In a seamless way. Although many adaptation techniques exist, selections of appropriate adaptations among multiple choices that would satisfy given constraints are often ad hoc. To provide a systematic solution, we present a general conceptual framework to model video entity, adaptation, resource, utility, and relations among them. It allows for formulation of various adaptation problems as resource-constrained utility maximization. We apply the framework to a practical case of dynamic bit rate adaptation of MPEG-4 video streams by employing combination of frame dropping and DCT coefficient dropping. Furthermore, we present a descriptor, which has been accepted as a part of MPEG-21 Digital Item Adaptation (DIA), for supporting terminal and network quality of service (QoS) in an interoperable manner. Experiments are presented to demonstrate the feasibility of the presented framework using the descriptor.

Effects of Low Air Temperature and Light Intensity on Yield and Quality of Tomato at the Early Growth Stage (정식 초기의 저온·저일조가 토마토 수량·품질에 미치는 영향)

  • Wi, Seung Hwan;Yeo, Kyung-Hwan;Choi, Hak Soon;Yu, Inho;Lee, Jin Hyong;Lee, Hee Ju
    • Journal of Bio-Environment Control
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    • v.30 no.4
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    • pp.448-454
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    • 2021
  • This study was conducted to the effect of low air temperature and light intensity conditions on yield and quality of tomato at the early stage of growth in Korea. Inplastic greenhouses, low temperature and low temperature with shade treatments were performed from 17 to 42 days after plant. Tomato growing degree days were decreased 5.5% due to cold treatment during the treatment period. Light intensity decreased 74.7% of growing degree days due to shade. After commencing treatments, the plant growth decreased by low temperature and low radiation except for height. Analysis of the yield showed that the first harvest date was the same, but the yield of the control was 3.3 times higher than low temperature with shade treatment. The cumulative yields at 87 days after transplanting were 1734, 1131, and 854 g per plant for control, low temperature, and low temperature with shade, respectively. The sugar and acidity of tomatoes did not differ between treatment and harvesting season. To investigate the photosynthetic characteristics according to the treatment, the carbon dioxide reaction curve was analyzed using the biochemical model of the photosynthetic rate. The results showed that the maximum photosynthetic rate, J (electric transportation rate), TPU (triose phosphate utilization), and Rd (dark respiration rate) did not show any difference with temperature, but were reduced by shading. Vcmax (maximum carboxylation rate) was decreased depending on the low temperature and the shade. Results indicated that low temperature and light intensity at the early growth stage can be inhibited the growth in the early stage but this phenomenon might be recovered afterward. The yield was reduced by low temperature and low intensity and there was no difference in quality.

EFfect of Feed Withdrawal Time on Salmonella Contamination in the Crop, Intestinal Weight, and Blood Parameters of Broilers before Harvesting (절식 시간에 따른 육계의 소낭 내 Salmonella 오염도, 소화관 길이, 혈액 성분, 계육 품질에 미치는 영향)

  • Kim, Hee-Jin;Kang, Hwan-Ku;Hong, Eui-Chul;Kim, Hyun-Soo;Son, Jiseon;You, Are-Sun;Kang, Bo-Seok;Jeon, Jin-Joo
    • Korean Journal of Poultry Science
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    • v.48 no.4
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    • pp.287-295
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    • 2021
  • In this study, we aimed to evaluate the impact of different feed withdrawal periods (2, 3, 4, 5, and 6 h) on Salmonella contamination in the crop, intestinal weight, and blood parameters of broilers before harvesting. A total of 100 broilers (initial live weight 1.906±0.06 kg) were randomly assigned to the same five treatment groups and broilers had access to water but no feed before catching. Feed withdrawal times were 2, 3, 4, 5, and 6 h prior to slaughter. Measurements included live weight, pre-slaughter weight, carcass weight, weight of gastrointestinal tract. In addition, Salmonella and Campylobacter contamination in the crop, serum biochemical profiles, counts of leukocytes, erythrocytes, and platelets, and meat quality were assessed. The results demonstrate that carcass weight, blood corticosterone, meat pH, meat color, and water holding capacity did not differ based on the feed withdrawal time. Carcass yield increased as the withdrawal time increased, but the weights of the crop, proventriculus, jejunum, ileum, and rectum were significantly reduced by 6 h (P<0.05). Salmonella counts were the lowest in the crops subjected to 6 h of feed withdrawal (P<0.05). Shear force was highest in chicken breast meat after 6 h of feed withdrawal (P<0.05). However, when the feed was withdrawn after 6 h, the shear force increased, but it did not affect the sensory characteristics in consumers, and the Salmonella count also decreased and had a reduction effect on contamination during slaughter. These findings can serve as reference data for setting the feed withdrawal time.

A study on the status of dietary behavior and nutrient intakes according to the gender and the level of physical activity of adolescents: using the 7th (2017-2018) Korea National Health and Nutrition Examination Survey (청소년의 성별과 신체활동 수준에 따른 식행동 및 영양섭취실태: 제7기 2,3차년도 (2017-2018년) 국민건강영양조사 자료 이용)

  • Seo Yeon Kim;Sun A Choi;Jeong Ok Rho
    • Journal of Nutrition and Health
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    • v.56 no.6
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    • pp.683-701
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    • 2023
  • Purpose: The purpose of the study was to analyze the status of dietary behavior and nutrition intakes according to the gender and the level of physical activity of adolescents. Methods: Data from 659 adolescents aged 13 to 18 years who participated in the Korea National Health and Nutrition Examination Survey (2017-2018) were analyzed. Collected data were analyzed using complex sampling data analysis with SPSS v. 27.0. Results: The adolescents were categorized into three groups based on their physical activity as follows: The first group with high physical activity (8.9%), a second group with normal physical activity (29.7%), and a third group without physical activity (61.4%). Boys showed significantly higher physical activity than the girls. Adolescents with high physical activity had a significantly lower body mass index than adolescents without physical activity, but their meal regularity, subjective health status, and recognition of body image were significantly higher. In terms of biochemical factors, adolescents with high physical activity had lower triglycerides and cholesterol than adolescents without physical activity, while hemoglobin and hematocrit were significantly higher. In addition, the intakes of energy, carbohydrates, proteins, fat, vitamin B1, niacin, calcium, phosphorus, sodium, potassium, iron increased significantly as adolescents' physical activity increased. Conclusion: The physical activity of adolescents had a positive effect on dietary behavior, lifestyle, and nutritional factors. Therefore, it is necessary to increase participation in physical classes in schools and develop various programs considering the level of physical activity among adolescents.

Improvement Effect of Fermented Orostachys malacophyllus against Orotic Acid-induced Fatty Liver Model Rats (발효 와송 급여 흰쥐의 orotic acid 유발 지방간 개선 효과)

  • Ahn, Hee-Young;Choe, Da-Jeong;Cho, Young-Su
    • Journal of Life Science
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    • v.25 no.8
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    • pp.917-924
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    • 2015
  • This study aimed to evaluate the protective effect of Orostachys malacophyllus (OM) and fermented O. malacophyllus (FOM) in Sprague-Dawley rats who had been intoxicated with 1% (w/w) orotic acid (OA) for 10 days. The activities of several hepatic enzymes, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and cholinesterase, were increased when OA was given, but these parameters were significantly decreased by FOM treatment. In addition, OA treatment resulted in an increased lipid peroxidative index (thiobarbituric acid-reactive substances, TBARS). A worsened antioxidant status (reduced glutathione) in the liver and serum was also observed. FOM treatment improved the antioxidant status of OA-induced fatty-liver rats, which was evaluated by decreased levels of the lipid peroxidative index and improved antioxidant status in the liver and serum. The contents of liver non-heme iron were increased with OA treatment and significantly decreased with FOM treatment, which suggested that the lipid peroxidation contents were inversely correlated with liver non-heme iron content. Based on these results, FOM is considered a material with significant potential for development into a functional health food that can improve fatty-liver conditions.

Effect of Cordycepin-Enriched Cordyceps militaris Powder on Tissues Lipid Peroxidation and Antioxidative Activity in Orotic Acid-Induced Fatty Liver Model Rats (Cordycepin이 orotic acid 유발 지방간 흰쥐의 과산화 지질 농도 및 항산화 활성에 미치는 영향)

  • Ahn, Hee-Young;Cha, Jae-Young;Heo, Su-Jin;Kang, Min-Jeong;Lee, Jae-Hong;Park, Sang-Hyun;Cho, Young-Su
    • Journal of Life Science
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    • v.21 no.10
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    • pp.1407-1414
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    • 2011
  • This study is aimed to evaluate the protective effect of Cordycepin-enriched Cordyceps militaris (CM${\alpha}$) strain on orotic acid (OA)-induced fatty liver in rats. OA treatment induced the retardation of body weight gain and enlargement of the liver. The activities of liver marker enzymes, alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), Alk. phosphatase and Cholinesterase were increased when treated with OA, but these parameters were significantly decreased in the CM${\alpha}$ group. The current study observed significant elevations of thiobarbituric acid-reactive substance (TBARS) levels. However, among the OA groups, the CM${\alpha}$ group showed significantly low TBARS levels of hepatic homogenate. The OA group resulted in a significant decrease in the levels of plasma and hepatic glutathione, but these reductions were significantly increased in the CM${\alpha}$ group. These effects were more pronounced in the CM${\alpha}$ group than in the PJ or CM groups in Orotic acid treated rats. Accordingly, Cordycepin-enriched Cordyceps militaris (CM${\alpha}$) may be an ideal candidate for hepatoprotective effects in animal models.