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http://dx.doi.org/10.4014/kjmb.1112.12002

Isolation and Identification of Agarose-degrading Bacterium, Pseudoalteromonas sp. GNUM08122  

Kim, Yu-Na (Department of Chemical & Biological Engineering and RIGET, Gyeongsang National University)
Jeong, Yeon-Kyu (College of Marine Science and Institute of Marine Industry, Gyeongsang National University)
Kim, Mu-Chan (College of Marine Science and Institute of Marine Industry, Gyeongsang National University)
Kim, Sung-Bae (Department of Chemical & Biological Engineering and RIGET, Gyeongsang National University)
Chang, Yong-Keun (Department of Chemical & Biomolecular Engineering, Korea Advanced Institute of Science and Technology)
Chi, Won-Jae (Division of Bioscience and Bioinformatics, Myungji University)
Hong, Soon-Kwang (Division of Bioscience and Bioinformatics, Myungji University)
Kim, Chang-Joon (Department of Chemical & Biological Engineering and RIGET, Gyeongsang National University)
Publication Information
Microbiology and Biotechnology Letters / v.40, no.1, 2012 , pp. 1-9 More about this Journal
Abstract
This study's aim was to isolate microorganisms producing agarase with a high activity, with possible applications in improving the performance of the pretreatment processes for bioethanol production. Marine algaes were collected from the south coast of Korea, from which three kinds of microorganisms were isolated. After a 4-day culture of these strains at $25^{\circ}C$, crude enzymes were obtained from culture supernatant or cell-free extract by ammonium sulfate precipitation and membrane dialysis. Agarase activity was observed in these crude enzymes. Notably higher specific activity was observed in the crude enzyme obtained from the culture supernatant rather than that from the cell-free extract. This indicates that a secreted enzyme has a much greater activity than a cellular enzyme. Crude enzymes from the GNUM08122 strain were inferred to have ${\alpha}$-agarase activity because release of p-nitrophenol was observed, possibly due to the cleavage of p-nitrophenyl-${\alpha}$-D-galactopyranoside. The 16S rRNA sequence of GNUM08122 showed a close relationship to Pseudoalteromonas issachenkonii KMM 3549 (99.8%) and Pseudoalteromonas tetraodonis IMA 14160 (99.7%), which led us to assign it to the genus Pseudoalteromonas. Biochemical and physiological study revealed that this strain can grow well at $40^{\circ}C$ under a wide range of pH (pH 4~8) in high-salt conditions (10% NaCl).
Keywords
Agarase; agar degradation; marine bacteria; isolation; Pseudoalteromonas sp.;
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