• Title/Summary/Keyword: 생육조사

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Induction of Mutants by Irradiation of $\gamma$-Ray on In vitro Shoots of Persimmon (기내$\gamma$-선 처리에 의한 감(Diospyros kaki Thunb.) 돌연변이 유기)

  • 고갑천
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.2
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    • pp.143-148
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    • 2000
  • This study was carried out to know the optimum dose of gamma-ray for the induction of mutation in vitro and the characteristics of the mutants induced by gamma-ray in persimmon (Diospyros kaki Thunb.). The LD50 (50% lethal dose) for in vitro shoots of the cultivar, Nishimurawase was between 1 krad and 2 krad and about 1 krad for the cultivar, Ichikikeijiro. As the dose of gamma-ray increased, the length of shoots decreased and necrosis of buds increased. For the cultivar, Nishmurawase, 37.5∼58.3% shoots rooted and the rooting rate and the number of roots per shoot was low in high gamma-ray. The irradiated young plants which were grown in the growth cabinet for 6 weeks were shorter in shoot length and had more branches than non-irradiated plants. The survival rate of irradiated plants grown in the green house for 3 months was 33%, while 77% for control plants.

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Comparison of Growth Condition of Abies koreana Wilson by Districts (지역별 구상나무 생육현황 비교)

  • 추갑철
    • Korean Journal of Environment and Ecology
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    • v.14 no.1
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    • pp.80-87
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    • 2000
  • 구상나무림 보전관리의 기초자료를 마련하고자 지리산 한라산 및 덕유산의 아고산지대에 95개의 조사구를 설치하여 한국특산종 구상나무의 생육현황과 구상나무 서식지의 입지인지를 조사하였다 구상나무는 흉고직경 10-30cm의 범위에 드는 나무들이 주로 고사목으로 나타났으며 고사목의 비율은 전체적으로 총개체수의 11.51%였다 고사목의 비율또한 덕유산에서 18.18%로 가장높게나타났고 한라산 지역이 8.11%로 가장 낮게 나타났다 조사구당 구상나무 개체수 치수의 개체수 구상나무의 수고와 흉고직경 생육현황표에 의한 점수 구상나무의 상대밀도 등은 지역간에 통계적 유의차가 인정되었다 생육중인 구상나무의 활력은 한라산 지리산, 덕유산으로 순으로 좋았다 조사구당 구상나무 치수의 개체수는 한라산에서 6.00개체로 가장 많았으며 덕유산에서는 치수가 없는 것으로 나타났다 구상나무의 상대밀도도 한라산, 지리산, 덕유산 순으로 높게 나타났다. 조사구당 구상나무 개체수는 해발고와 정의 상관, 방위, 낙엽퇴의 두께, 조사구당 교목의 개체수 및 종수, 활엽수 상대밀도와는 부의 상관이 인정되었다. 구상나무 상대밀도는 해발고와는 고도의 정의 상관관계 방위 조사 구당교목의 개체수 및 종수 수관울폐도, 활엽수 상대밀도와는 부의 상관관계가 인정되었다 지리산, 한라산 및 덕유산에 자생하는 구상나무는 해발고가 높으며 볕이 잘들고 낙엽퇴가 얕으며 종구성이 단순하고 활엽수가 적은 지역에 보다 많이 분포한다.

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Analysis of Growth Environment on Old Tree, a Natural Monument in Gyeongsangbuk-do (경북지역의 천연기념물 노거수 생육현황 분석)

  • Kim, Tae Sik;Cho, Sun Hee;Kang, Hee Jin
    • Korean Journal of Heritage: History & Science
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    • v.43 no.1
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    • pp.190-211
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    • 2010
  • The purpose of this paper is to provide the preliminary data for maintenance of old trees of natural monuments and establishing protection projects. Accordingly, growth conditions, location circumstances, environment and protection management were analyzed for old trees at 34 sites in Gyeongsangbuk-do. Tree forms, crown, trunk and trees' health, leaves volume were analyzed in survey sites. As the results, the growth of Picrasma quassioides in Songsadong, Andong-si and Catalpa ovata in Hongwon-ri, Cheongsong-gun were not in good condition, along with other old trees of 6 cities (Quercus variabilis in Susan-ri, Uljin-gun, Juniperus chinensis in Hujeong-ri, Uljin-gun, Pyrus ussuriensis in Ssangjeon-ri, Uljin-gun, Salix glandulosa var. pilosa in Deokchon-ri, Cheongdo-gun, Pinus densiflora for. multicaulis in Hwasan-ri, Mungyeong-si, Pinus densiflora in Daeha-ri, Mungyeong-si). Accoding to these results, protection project and consistent monitoring are necessary. The analysis results of growth conditions per location circumstances showed that the old trees in the small spaces with large traffic of people were in bad condition. Therefore, it is necessary to secure sufficient space and soil improvement for such trees. The analysis results of growth conditions per species showed that Zelkova serrata, Quercus spp., Pinus densiflora are in good condition while Salix koreensis and others, i.e. a singular tree, are in poor condition. Thus, relatively smaller individuals and separately situated trees need careful management in their protection. The analysis results of trunk's growth ratio showed that Pinus densiflora, Ginkgo biloba, Zelkova serrata are in good condition whereas Juniperus chinensis, Quercus variabilis, Salix koreensis are not. It is a recent trend that tree surgeries are gradually decreasing; there are a number of damaged trees with no maintenance/management, and several sites requiring mend trunks management and cabling, bracing in protection and management projects. Hence, in the future, local government and management organizations should plan for systematic management and establish a protection plan for the natural monument trees. Also, 12 sites were found to be in need to purchase lands in order to secure the growth spaces, and since most of these areas are available for purchase, it is needed to promote a budget and purchase those areas through persuasion of the owner. These areas seems crucial in order to maximize the effect of conservation efforts and budgets for the preservation and restoration of the old trees.

Effects of Irradiation and Cold Storage on Rooting and Growth of Chrysanthemum Cuttings (방사선조사와 저온저장이 국화 삽수의 발근 및 생육에 미치는 영향)

  • 김진규;노설아;신해식;송희섭;최병진
    • Korean Journal of Environmental Biology
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    • v.20 no.4
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    • pp.355-359
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    • 2002
  • This study was carried out to investigate the combined effect of cold storage with irradiation on rooting of chrysanthemum cuttings. Each cutting was six cm in length with three real leaves. A set of Groups of the cuttings was irradiated with 0 to 70Gy of gamma-ray after four weeks of cold storage, while another groups were irradiated with the same doses and then stored at $4^\circ{C}$ for four weeks. Root and shoot growth were observed at 15 and 20 days after planting of the cuttings in a Plug tray. Accelerated root formation was observed in 30 Gy irradiated group, and root growth was also enhanced in 20 and 30 Gy irradiated groups 20 days after planting. In case of irradiation combined with pre-treatment of cold storage, survival rate of cuttings was the highest (80%) in 30 Gy irradiated group. The groups treated with irradiation first before cold storage were apparently better in root formation, among which 50 Gy irradiated group showed the highest rate of root growth.

Effects of low dose gamma radiation on the early growth and physiological activity of gourd(Lagenaria leucantha L.) (저선량 방사선이 참박의 초기생육과 생리활성에 미치는 효과)

  • Kim, Jae-Sung;Lee, Young-Keun;Park, Hong-Sook;Back, Myung-Hwa;Chung, Kyu-Hoi
    • Korean Journal of Environmental Agriculture
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    • v.19 no.2
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    • pp.142-146
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    • 2000
  • To observe the stimulating effects of low dose ${\gamma}-radiation$ on the seed germination, early growth and physiological activity in the gourd, seeds of three cultivars (Partner, Support, FR Yongja) were irradiated at the dose of $0.5{\sim}30\;Gy$. The hormetic effects of the low dose ${\gamma}-radiation$ on the growth were different from each cultivar. Low dose ${\gamma}-radiation$ had promoting effects on the germination at the optimum dose of 2 Gy and 8 Gy and on the early growth at the optimum dose of 4 Gy, 16 Gy and 20 Gy in Partner and Support cultivar and 1 Gy in FR Yongja cultivar. Irradiation didn't have significant effects on the chlorophyll contents of gourd plantlet. The antioxidant enzyme activity of gourd plantlet and early growth increased in low dose irradiation group.

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Development of Functional Drink Using the $\textit{Hericium erinaceum}$ Cultivated on the $\textit{Angelica keiskei}$ (신선초를 이용한 $\textit{Hericium erinaceum}$ 음료 개발에 관한 연구)

  • 권상철;조주현;정재현
    • Food Industry And Nutrition
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    • v.8 no.3
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    • pp.45-51
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    • 2003
  • 본 연구는 생물공학적인 방법을 도입하여 폐기되는 신선초박에 H. erinaceum의 균사체를 발효시킨 배양생성물을 이용하여 기능성 건강 음료 개발을 검토 하고자 하였다. 1. 종균제조공정개발 : 기본 배지의 선발에서 Hericium erinaceum의 균사 생육에 적합한 배지를 선발하기 위하여 10여종의 고체배지를 사용하여 균사 생육 및 밀도를 조사한 결과는 YMPG 배지에서 59.8mm/14days로 균사 생육이 가장 우수한 것으로 나타났고, 최적 온도는 20-$25^{\circ}C$ 범위에 가장 생육이 좋았으며, 배지의 pH를 조절하여 균사생육을 조사한 결과, pH는 5.5, 접종비는 전배양액 9%(v/v), 배양에 적합한 배지액량은 50mL, 최적교반속도는 120rpm이었다. 이러한 최적 조건 하에서 배양 경시 변화를 살펴 본 결과 당은 거의 일정한 속도로 감소하는 반면에 건조균체량은 배양 8일째까지 증가하다가 더 이상 변화가 없었다. 2. 발효공정개발: 수분함량이 200%(v/v)에서, pH5에서의 생육속도는 90mm/30 days, $25^{\circ}C$에서의 균사생육속도는 89mm/30days로 각각 H. erinaceum균사의 생육이 가장 우수한 결과를 얻었다 3.추출공정 및 시제품 제조: 녹즙을 생산한 후 폐기되는 신선초박에 액체 종균을 접종하여 ,40일 동안 배양시켜 생육 상태가 우수한 배양생성물만을 선별하여, 열수추출방법으로 10$0^{\circ}C$, 10시간 추출한 것을 음료제조의 원료로 하고, 음료의 기호성을 향상시키기 위해 유기산 및 한방추출물을 첨가하는 균사체음료의 조성비를 얻었다.

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Effect of Light Quality on Growth of Cucumber Plug Seedlings (광질이 오이 플러그 묘의 생육에 미치는 영향)

  • 용영록;황세진;김일섭
    • Proceedings of the Korean Society for Bio-Environment Control Conference
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    • 2000.10b
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    • pp.89-93
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    • 2000
  • 광파장 및 조사시기에 따른 오이묘의 생육 반응을 검토하였다. 광원에 따른 오이 묘의 생육상태를 조사해본 결과 청색광, 적색광을 처리한 것이 생체중, 하배축 무게, 근중이 증가하면서 하배축의 신장이 억제되었고, 청색광과 적색광을 혼합하여 야간 12시간씩 조사하여 30일간 육묘해 본 결과 뿌리의 발육도 우수하고, 줄기와 잎의 생육 정도에서도 우량묘의 소질을 보였다. 청색광과 적색광을 혼합하여 야간 6시간과 12시간씩을 조사하여 30일간 육묘한 결과 하배축의 길이는 각각 60.0mm와 44.9mm였고, 하배축의 무게는 0.59g, 0.62g으로 나타나 12시간 조사구가 하배축의 신장억제효과가 큰 것으로 나타났다. 하배축의 엽록소 함량은 청색광과 적색광의 조사시간이 길어질수록 뚜렷이 증가하였고, 식물체도 진한 녹색을 나타냈다. 광처리구와 무처리구의 오이묘 하배축의 횡단면과 종단면을 검경하여 세포구조를 비교해 본 결과 광처리구의 횡단면 표피조직은 단층의 밀착된 세포로 되어 있으며, 바깥쪽 세포는 현저하게 두꺼웠다. 후각조직의 세포는 작고 부정형이며, 세포간극이 조밀하였으며, 세포구조도 치밀한 특성을 보였다. 종단면은 광처리구에서 세포가 짧고 세포간에 간극이 없는 반면 무처리구는 세포가 길고 뚜렷이 구분되지 않은 것이 관찰되어 대체로 광처리구는 무처리구에 비해 세포신장이 억제되는 대신 세포구조가 치밀하여 식물체의 도장이 억제된다는 것을 알 수 있었다.

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Studies on the Wholesomeness of Gamma-irradiated Rice -(II) On the Weight Gain, Reproduction Ratio, Mortality and Growth Rate after Weaning of Mice- (방사선 조사미(照射米)의 안전성(安全性) 연구 -제2보 시험동물의 증체량(增體量), 번식률(繁殖率), 사망율(死亡率) 및 이유시(離乳時) 생육상태(生育狀態)-)

  • Kim, Sung-Kih;Lee, Kwan-Young;Lee, Su-Rae
    • Korean Journal of Food Science and Technology
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    • v.5 no.3
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    • pp.149-152
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    • 1973
  • As the third-year experiment on the wholesomeness of irradiated rice with 50 and 100 krad gamma-rays, a feeding test was undertaken with mice of $F_2-generation$ which were used in the preceding year. No significant difference was observed between the irradiated group and control group with respect to gain of body weight, reproductive ratio, mortality and average body weight of weaning mice.

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Effects of low dose $\gamma$-ray on the early growth of tomato and the resistance to subsequent high doses of radiation (저선량 $\gamma$선 조사가 토마토의 초기생육과 후속고선량 $\gamma$선 저항성에 미치는 영향)

  • Kim, Jae-Sung;Kim, Jin-Kyu;Back, Myung-Hwa;Kim, Dong-Hee
    • Journal of Radiation Protection and Research
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    • v.24 no.3
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    • pp.123-129
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    • 1999
  • Tomato (Lycopericum esculentum $M_{ILL}$ cv. Seokwang and cv. Housemomotaro) seeds were irradiated with the doses of $1{\sim}20$ Gy from $^{60}Co$ $\gamma$-ray source to investigate the effect of the low dose $\gamma$-ray radiation on the early growth and resistance to subsequent high dose of radiation. Germination rate of seeds irradiated with low dose $\gamma$-ray was enhanced in Seokwang cultivar but not in Housemomotaro cultivar. Seedling height increased in 4 Gy and 8 Gy irradiation group of both cultivars. Plant height of Seokwang cultivar was depressed in low dose irradiation group but fresh weight was increased in 2 Gy and 4 Gy irradiation group. In Housemomotaro cultivar, plant height increased in 12 Gy and 20 Gy irradiation group and fresh weight increased in 4 Gy and 20 Gy irradiation group. Growth inhibition of tomato plants by high dose radiation was noticeably reduced by pre-irradiation of low dose radiation. Resistance to subsequent high dose of radiation was enhanced in 2 Gy and 8 Gy Irradiation group of Seokwang cultivar and in 2 Gy and 12 Gy irradiation group of Housemomotaro cultivar.

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