• Korean Journal of Microbiology
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• v.37 no.4
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• pp.299-304
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• 2001

Teicoplanin is a kind of glycopeptide antibiotics produced by Actinoplanes teichomyceticus, and used in the clinical antibiotic such as vancomycin against methicillin-resistant Stabphylococcus aureus (MRSA). Actino planes teichomyceticus ATCC 31121 was mutated with UV to obtain a superior mutant strain with increased level of teicoplanin production. In this investigation, lethal curve was obtained and the optimal condition to induce mutagenesis was determined to isolate the desirable mutant strain. It was also confirmed that teicoplanin activities by agar diffusion method was compared with the parent strain. One mutant strain, T991014-1 with the highest productivity, was finally selected, and was characterized through the various tests such as amylase activity, protease activity, halotolerance, antibiotic resistance, autotoxicity, and productivity. Ad fermentation characteristics of the mutant strain were also studied.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.04a
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• pp.493-496
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• 2008

Recently, shortage of natural aggregates resources and the amount of waste concrete hsve been rapidly increased, the technique of aggregates manufacturing of waste concrete is damanded. In this study, we evaluated the performance of gutter type roll crusher. Gutter type roll crusher is showed enhanced producing manufacturing ability. The gutter type roll crusher achieved more friction energy than general roll crusher, also a mount of producing showed same results.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.233-235
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• 2003

The phospholipase C (PLC) hydrolyzes the polar head groups such as phosphocholine or phosphoethanolamine residues esterified at the sn-3 position of phospholipids. Pichia pastoris can utilize methanol as a carbon source and produce recombinant proteins under the control of the strong, tightly-regulated alcohol oxidase (AOX) promoter. In this study, we developed recombinant P. pastoris system for the high productivity of PLC and analyzed PLC activity.

• Korean Journal of Microbiology
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• v.30 no.3
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• pp.213-217
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• 1992

Glucosyltransferase of Streptococcus mutans NCTC 10449 was purified and characterized. It relates with production of insoluble glucan in dental caries. The molecular weight was estimated to be 152.000. The optimum pH and temperature was 6.5 and $35^{\circ}C$. respectively. The enzyme was stable in alkaline pH. The Michaelis constants of the enzyme for releasing of fructose were 48 mM. Hydrolysis rate of insolut~le glucan by tlextranase was higher in S. mutuns NCTC 10449 than that of strains isolated from saliva.

• Microbiology and Biotechnology Letters
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• v.20 no.1
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• pp.34-39
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• 1992

For the screening of new-functional and specific exopolysaccharide, a bacterium strain was isolated from soil through the two steps of screening. The isolated bacterium was identified as Bacillus polymyxa KS-1 according to the criteria of morphological, physiological, and chemical taxonomic analyses. The exopolysaccharide was composed of glucose:galactose: mannose and galactosamine in an approximate molar ratio of 1.00:0.36:1.02:1.10. The produced exopolysaccharide by Bacillus polymyxa KS-1 was found to be revealed new acidic polysaccharide which did not contain pentose, ketose, starch, and uronic acid.

• The Research Journal of the Costume Culture
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• v.18 no.3
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• pp.588-598
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• 2010

Purposes of this research were to examine characteristics of contractors and the relationship between contractors and wholesalers of apparel products. Influence of such variables on wholesalers' satisfaction was also assessed. Location, reputation and service were factors of contractor characteristics. Dependence, cooperation and power were three factors that were considered for contractor-wholesaler relationship variable. Survey data from 109 wholesalers located in Dongdaemun Fashion Town in Korea was used for analysis. Empirical results confirmed the importance of service factor of contractor characteristics. Service and location of the contractor had positive influence on cooperation. Location had negative influence on power. Reputation had positive direct influence on wholesaler satisfaction. Cooperation between contractors and wholesalers had significant influence on wholesaler satisfaction.

• Proceedings of the Korea Concrete Institute Conference
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• 2009.05a
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• pp.339-340
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• 2009

this study, after examining quality of mortar for different manufacturing stages of recycled sand produced by wet gravity separation system, it was found that the quality of recycled sand was improved greatly after going through the wet gravity separation system.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 1994.07a
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• pp.13-14
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• 1994

제주지역에서 생산되는 대표적인 품종인 궁천조생과 흥진조생에 대한 품질에 관여하는 물리.화학적인 특성을 분석하였고 과일의 크기, 색깔, 생산지역에 따른 감귤의 맛등에 대한 관능검사를 실시하였다. 수확적기에 수확한 조생온주으 가용성고형물은 10.7, 11.0 였으며 산함량은 1.04%, 비타민 C함량은 41.19-44.94mg/100g였다. 당은 절반이 자당하이였고, 포도당과 과당이 각각 1/4씩 함유하고 있었다. 과일 크기에 따라 과중, 껍질두께, 가용성고용물, pH, 산함량, 산비는 각각 직선적인 상관관계를 가지고 있었으며, 경도와 과육율과의 유의성은 없었다. 관능검사 결과 중간정도의 크기의 감귤을 선호하며. 껍질의 색깔은 진한 홍등색일수록 선호하고 있음을 알 수 있었다. 또한 같은 품종이라고 할지라도 재배적지에서 생산된 감귤일수록 선호도가 높아 직선적인 유의성을 보였으며, 과즙의 경우 당산비가 높을수록 성호하여 고품질 감귤생산을 위한 노력이 필요한 것으로 여겨졌다.로 여겨졌다.

• Proceedings of the KIPE Conference
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• 2008.06a
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• pp.100-102
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• 2008

앞으로의 에너지 고유가 시대를 대비하여 수소의 생산에 대한 연구를 활발히 진행되어지고 있다. 여러 가지 수소를 생산하는 방법 중 본 연구에 사용되는 방법은 개질반응법으로써 스팀(수증기)개질 방식을 사용하는 1KW용 개질시스템에 관한 연구이다. 본 장치는 천연가스를 이용하여 1m3/hr을 생산하는 수증기 개질시스템이다. 크게 세가지로 나누어 지는데, 첫째 시스템 제어부, 둘째 가스 및 물 공급부, 셋째 개질기이다. 본 연구에서는 이 개질 시스템의 운전 특성과 모니터링에 대해서 알아보고자 한다.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.23 no.54
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• pp.45-53
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• 2000

본 연구에서는 공구이송장치(Tool Transporter)의 이용횟수를 최소화하는 공구교환문제(Tool Switching Problem)를 다룬다. 한 기계에서 생산해야 하는 제품들이 요구하는 공구의 총수가 기계에 장착할 수 있는 공구의 수보다 많은 경우 각 제품을 생산할 때마다 공구이송장치를 이용한 공구교환이 필요하다. 공구저장소(Tool Crib)와 기계간에 한 번에 이송할 수 있는 공구의 수가 제한되어 있기 때문에 공구이송장치의 이용률에 따라서 기계의 생산성이 크게 달라진다. 그러나 기존의 연구에서는 공구이송장치의 능력을 배제하고 공구교환횟수만을 고려하고 있다. 따라서 본 연구에서는 공구이송장치의 이용횟수의 최소화를 목적함수로 하는 공구교환문제를 수리적으로 모형화하고 이 문제에 대해 기존의 최적 공구교환방법을 적용하여 얻어지는 해는 최적해를 보장할 수 없다는 점과 공구가 필요한 시점보다 먼저 장착하는 경우(Early Insertion)를 고려할 때 기존 방법에 의한 해보다 좋은 해를 얻을 수 있다는 특성을 간단한 예를 통하여 보여준다. 또한, 이러한 특성을 고려한 새로운 공구교환방법을 제시하고 다양한 유형의 예제를 통하여 기존의 방법과 비교한다.