• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.76-76
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• 2018

화학적 살충제는 빠르고 정확하게 해충을 방제하는 효과를 나타내지만, 인간과 다른 유기체에 대한 살충제 잔류의 위험성, 화학적 살충에 대한 저항성을 가진 해충의 출현, 생태계의 교란 등 다양한 부작용이 있다. 이 때문에 생물적 방제를 통한 해충 방제는 효과적인 수단이 될 수 있다. 특히 채소 작물 등에 주로 발생하는 응애는 생산량 저하 및 품질 저하 등과 같은 다양한 문제를 일으킬 수 있다. 특히 진딧물이나 점박이응애는 화학적 살충제에 대해 높은 저항성이 발달되어 문제가 되고 있다. 본 연구에서는 아이비(Hedera helix), 란타나(Lantana camara), 예루살렘체리(Solanum pseudocapsicum)를 통한 천연 식물 추출물의 응애에 대한 생물적 방제 효과를 확인하였다. 각 추출물은 0.5%, 0.05% 주정 및 메탄올로 추출하였으며, 응애 방제 효과(%)는 아이비>란타나>예루살렘체리로 나타났다. 각 추출물은 GC/MS 분석을 통해 생리활성 후보 물질을 확인하였다. 이를 통해 농업 이익 증대, 생산량 유지와 더불어 환경 및 인간과 다른 유기체에 피해를 주지 않으며 효과적으로 응애를 방제 할 수 있는 천연 생물적 방제제로의 가치를 확인하였다.

• Korean Journal of Environmental Biology
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• v.27 no.3
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• pp.261-265
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• 2009

Litter production, nutrient concentration of each component of litterfall and amount of nutrients input to forest floor via litterfall were investigated for three years from May 2005 through April 2008 in Pinus densiflora forest at Mt. Worak National Park. Amount of litterfall in 2005, 2006, 2007 were 3.070, 3.066, 3.099 ton $ha^{-1}yr^{-1}$, respectively. Average amount of litterfall for three years was $3.078{\pm}0.018\;ton\;ha^{-1}yr^{-1}$. Average percentage of leaf litter, branch and bark, reproductive organ and the miscellaneous for three years were 61.9, 10.4, 5.2, 22.5%, respectively. Average amount of N, P, K, Ca and Mg returned to forest floor via litterfall for three years in this P. densiflora forest were 18.014, 0.878, 4.240, 7.349 and 2.172 kg $ha^{-1}yr^{-1}$, respectively.

• KSBB Journal
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• v.19 no.3
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• pp.192-199
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• 2004

In this work the production of glutathione (GSH) by yeast Saccharomyces cerevisiae and the monitoring of the process were studied. In shaking culture the production of GSH was high at initial pH value of 4 and at temperature of 30$^{\circ}C$. But when L-cysteine was added to the culture medium at the beginning of the cultivation, the productivity of GSH was low. In case 0,5% (v/v) of L-cysteine, glycine and glutamic acid were introduced to the culture medium in the exponential cell growth phase, high concentration of GSH (about 90 mg/L) was produced in the bioreactor. A fed-batch operation with stepwise glucose feeding strategy allowed to produce 102 mg/L of GSH. The cultivation processes were on-line monitored by a 2-dimensional fluorescence sensor. A few off-line data such as cell growth, cystein concentration, phosphate concentration and GSH productivity could be well correlated to the fluorescence intensity of some combinations of excitation and emission wavelengths.

• Korean Journal of Environmental Biology
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• v.20 no.4
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• pp.378-385
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• 2002

Growth of Pacific oystey, Crassostrea gigas, in Kamak Bay, Korea was modeled using Von Bertalanffy growth function, seasonal Von Bertalanffy growth function and generalized growth equation of Schnute and Richards' growth model, based on shell length and wet weight frequency data of 9208 oysters. Carrying capacity in the oyster culture ground was also estimated using Schaefer's and Fox's surplus production model. The present results suggest that the generalized growth equation of Schnute and Richards' model is fitter to describe the length growth pattern of C. gigas than Von Bertalanffy growth functions. This results also suggest that the current number of culture facility per unit area in 2000 is similar to the number of facility that produces the maximum production of oyster per unit area.

• Journal of the Korean Wood Science and Technology
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• v.30 no.1
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• pp.72-78
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• 2002

Cultivation of Pholiota adiposa on sawdust media and its mixed substrates with various agricultural by-products was attempted. The composition of each substrates were compared from the viewpoint of cultivation possibilities with the production of Pholiota adiposa. Populus deltoides showed the best production of the mushroom with 795 g, followed by Robinia pseudo-acacia and Quercus acutissima. The mixed sawdust media with 70% of Robinia pseudo-acacia and 30% of Populus deltoides showed the best production of the mushroom with 265 g followed by the mixture of Robinia pseudo-acacia and Populus deltoides with 50% each with 228 g, while the addition of Quercus acutissima showed the negative effect for the production of Pholiota adiposa. Thus, Robinia pseudo-acacia was considered to be an effective amendment for the production of the mushroom. In addition, various kinds of agricultural by-products showed additive effect for the production of the mushroom, too. Therefore, we could concluded that the sawdust of Robinia pseudo-acacia and agricultural by-products can be used to cultivate Pholiota adiposa.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.289-292
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• 2003

Effects of immobilization on the production of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) by Nicotiana tabacum cells were investigated using alginate and polyurethane foam as immobilization matrices. Encapsulation of the cells in alginate decreased protein production by 50% compared with that of suspension culture. Maximum hGM-CSF concentration was obtained by the cells immobilizaed in polyurethane foam. High hGM-CSF production could be possible when polyurethane foam was used because of high specific production and easy immobilization for cell recycling process with high cell density.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.380-383
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• 2000

The effect of on the cell growth, the elaboration of pullulan, the morphology and were the effect of on the molecular weight of pullulan were investigated. A. pullulans showed maximum pullulan production when initial pH 6.5 was 11.98 g/l in shake-flask culture. In batch culture, the maximum pullulan production of 15.16 g/l was obtained at an aeration rate of 0.5 vvm. The mixture of yeast-like form and mycelial form of cells was found at the constant pH 4.5, at which condition, the elaboration of pullulan was high, about 13.31 g/l. However, pullulan with its higher molecular weight (>1,000,000) was produced at the constant pH 6.5.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.321-324
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• 2002

In situ recovery of recombinant human granulocyte-macrophage colony stimulating factor (hGM-CSF) was performed in transgenic Nicotiana tabacum cell suspension cultures. Aqueous two-phase systems (ATPS) were used to utilize its biocompatibility. Transgenic plant cells could be grown up to 15.7 g/L in normal medium and 18.6 g/L in ATPS composed of 6% (w/w) polyethylene glycol (PEG) 20,000 and 10% (w/w) dextran 2,000,000. It was proved that the A TPS was not harmful to cell growth. In addition, It is expected to recover hGM-CSF simultaneously with cell growth. Using this method, maximum hGM-CSF concentration at 1.64 ng/mL was obtained on day 3.

• KSBB Journal
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• v.5 no.2
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• pp.101-106
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• 1990

The aspects of pullulan production by Aureobasidium pullulans were investigated under various initial pH, carbon source and nitrogen source conditions. The resulting pullulan fermentation broths were analyzed by using GC, LC and GPC techniques. The maximum pullulan production was obtained in the culture medium containing 5% sucrose at pH 6, 28$^{\circ}C$ after 7 days of cultivation. Under the pH 3, pullulan was almost not produced although the total cell mass of A. pullulans was increased, and the case on using (NH4)SO4 as a nitrogen source, which usually cause the fermentation medium under pH 3, also gave the similar phenomena. Sucrose was believed to converted to trisaccharide and glucose extracellulary and polymerization of glucose was proceeded intracellulary.

• KSBB Journal
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• v.6 no.4
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• pp.369-377
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• 1991

The effects of nitrogen sources(sodium glutamate and peptone) on the production of xanthan gum were investigated. The fermentation using sodium glutamate as a nitrogen source is longer than that of peptone. In the initial nitrogen concentration of 0.4-1.0g/L, Bs was about 2.0 and ${\beta}$s was 1.2. The optimal yields were obtained when the carbon source/nitrogen concentration was 10-16. The fermentation time and product yields in the fermentation medium of mixed nitrogen source [sodium glutamate-N(0.75g/L)+peptone-N(0.25g/L)] were similar to those of peptone.