Browse > Article

Production of Glutathione by yeast and Process Monitoring  

김춘광 (전남대학교 공과대학 물질ㆍ생물화학공학과, 생물공정기술연구실)
이종일 (전남대학교 공과대학 응용화학공학부, 생물공정기술연구실)
Publication Information
KSBB Journal / v.19, no.3, 2004 , pp. 192-199 More about this Journal
Abstract
In this work the production of glutathione (GSH) by yeast Saccharomyces cerevisiae and the monitoring of the process were studied. In shaking culture the production of GSH was high at initial pH value of 4 and at temperature of 30$^{\circ}C$. But when L-cysteine was added to the culture medium at the beginning of the cultivation, the productivity of GSH was low. In case 0,5% (v/v) of L-cysteine, glycine and glutamic acid were introduced to the culture medium in the exponential cell growth phase, high concentration of GSH (about 90 mg/L) was produced in the bioreactor. A fed-batch operation with stepwise glucose feeding strategy allowed to produce 102 mg/L of GSH. The cultivation processes were on-line monitored by a 2-dimensional fluorescence sensor. A few off-line data such as cell growth, cystein concentration, phosphate concentration and GSH productivity could be well correlated to the fluorescence intensity of some combinations of excitation and emission wavelengths.
Keywords
L-Cysteine; glutathione; monitoring; Saccharomyces cerevisiae; 2-dimensional fluorescence sensor;
Citations & Related Records
연도 인용수 순위
  • Reference
1 /
[ Colowick, S. P.;N. O. Kaplan ] / Methods in Enzymology
2 Optimal Production of Glutathione by Controlling the Specific Growth Rate of Yeast in Fed-Batch Culture /
[ Shimizu, H.;K. Araki;S. Shioya;K. I. Suga ] / Biotechnol. Bioengin.   DOI
3 Glutathione /
[ Meister, A. ] / Annual Rev. Biochem.
4 Analytical methods to investigate glutathoine and related compounds in biological and pathological processes /
[ Emanuela, C.;P. Mauro ] / J. chromatography
5 Effect of amino acids on glutathione production by Saccharomyces cerevisiae /
[ Alfafara, C. G.;K. Miura;H. Shimizu;S. Shioya;K. I. Suga ] / Appl. Microbiol. Biotechnol.
6 Studies on the production of Glutathione by Microorganism /
[ Cho, W. D.;H. I. Kim;J. C. Song;H. C. Yang ] / Kor. J. Appl. Microbiol. Biotechnol.
7 Cysteine addition strategy for maximum glutathione production in fed-batch culture of Saccharomyces cerevisiae /
[ Alfafara. C. G.;K. Miura;H. Shimizu;S. Shioya;K. I. Suga ] / Appl. Microbiol. Biotechnol.
8 Isolation, Identification and Culture Condition of Microorganism Producing Glutathione /
[ Shin, W. C.;D. S. Kim;J. H. Yu; J. H. Yu ] / Kor. J. Appl. Microbiol. Biotechnol.
9 Effect of additives and fed-batch culture strategies on the production of glutathione by recombinant Escherichia coli /
[ Li, Y.;J. Chen; Y. Y. Mao;S. Y. Lun;Y. M. Koo ] / Process Biochem.   DOI   ScienceOn
10 Medium optimization for glutathione production by Saccharomyces cerevisiae /
[ Liu, C. H.;C. F. Hwang; C. C. Liao ] / Process Biochem.   DOI   ScienceOn
11 Two-dimensional fluorescence spectroscopy : A new tool for on-line bioprocess monitoring /
[ Marose, S.;C. Lindemann;T. Scheper ] / Biotechnol. Prog.   DOI   ScienceOn
12 Monitoring of biological processes by 2-dimensional fluorescence sensor /
[ Rhee, J. I.;S. Y. Chung;K. H. Seo ] / Kor. J. Biotechnol. Bioeng.
13 On-line bioprocess monitoring with a multi-wavelength fluorescence sensor using multivariate calibration /
[ Skibsted, E.;C. Lindemann;C. Roca;L. Olsson ] / J. Biotechnol.   DOI   ScienceOn
14 Cysteine addition strategy for maximum glutathione production in fed-batch culture of Saccharomyces cerevisiae /
[ Alfafara, C. G.;K. Miura;H. Shimizu;S. Shioya;K. I. Suga ] / Appl. Microbiol. Biotechnol.
15 A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding /
[ Bradford, M. M. ] / Anal. Biochem.   DOI   ScienceOn
16 Flow-injection determination of copper (Ⅱ) based on its catalysis on the redox reaction of cysteine with iron(Ⅲ) in the presence of 1,10-phenanthroline /
[ Teshima, N.;H. Katsumata;M. Kurihara;T. Sakai;T. Kawashima ] / Talanta   DOI   ScienceOn