• 한국전기화학회:학술대회논문집
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• 2005.07a
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• pp.359-364
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• 2005

• Food Science and Preservation
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• v.24 no.7
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• pp.885-890
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• 2017

For the construction of the microbial monitoring method, anti-Salmonella polyclonal antibodies (pAbs) were produced from a rabbit and purified by saturated ammonium sulfate precipitation and protein A affinity column. The reactivity of anti-Salmonella pAbs was compared to that of commercial ones by using an indirect ELISA. The specificity of anti-Salmonella pAbs was investigated using 20 Salmonella serotypes and 20 non-Salmonella strains. A capturing ability of anti-Salmonella pAbs was investigated by exposing antibody-immobilized gold biosensor to different concentration of Salmonella mixture. Anti-Salmonella pAbs were successfully produced and purified with an antibody concentration of 2.0 mg/mL The reactivity of purified anti-Salmonella pAbs was greater than that of commercial one at all tested concentrations. All Salmonella serotypes, except S. Diarizonae, showed excellent binding efficiency with purified anti-Salmonella pAbs. Moreover, the purified anti-Salmonella pAbs showed excellent specificity against all non-Salmonella strains. The anti-Salmonella pAbs immobilized on the gold biosensor demonstrated the successful capturing capability against Salmonella with a dose-response manner. Therefore, the anti-Salmonella pAbs exhibited sufficient reactivity, specificity, as well as capturing capability against Salmonella to be considered as a bio-recognition element.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.05a
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• pp.381-382
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• 2001

다슬기 (Semisulcospira libertina libertina)는 식용으로 기호도가 높고 유용 수산자원으로서 개발 가치가 높으나 성장이나 자원량에 관한 기초 생물학적인 조사는 충분히 이루어지지 않고 있다. 최근에는 중금속, 농약 등에 오염될 가능성이 있어 자연산 다슬기의 식용 가능성 여부가 불투명한 상태에 이르고 있으며, 자연자원도 급격히 줄어 들고 있는 실정이다. 따라서 다슬기의 종묘생산 및 양식기술 개발에 대한 관심이 고조되고 있으며, 이 종에 대한 번식생물학적인 연구 (Chang et al., 2000)가 진행되고 있으나, 다슬기 종묘의 성장 상황에 대한 기준 자료는 찾아보기 힘들다. (중략)

• KSBB Journal
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• v.20 no.6
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• pp.393-400
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• 2005

Hydrogen($H_2$) as a clean, and renewable energy carrier will be served an important role in the future energy economy. Several biological $H_2$ production processes are known and currently under development, ranging from direct bio-photolysis of water by green algae, indirect bio-photolysis by cyanobacteria including the separated two stage photolysis using the combination of green algae and photosynthetic microorganisms or green algae alone, dark anaerobic fermentation by fermentative bacteria, photo-fermentation by purple bacteria, and water gas shift reaction by photosynthetic or fermentative bacteria. In this paper, biological $H_2$ production processes, that are being explored in fundamental and applied research, are reviewed.

• Korean Chemical Engineering Research
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• v.45 no.6
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• pp.529-535
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• 2007

Recently, an attempt to analyze and modify metabolic networks of living organisms in global level emerged with the benefit of development of high-throughput techniques, and it is generally called systems biology. Various systems biology studies have been carried out for the development of enhanced metabolite production systems. By modification of metabolic characteristics of microorganisms, metabolite productivities and yields obtained with metabolically engineered bacteria increased significantly compare with that obtained with wild type bacteria.

• Microbiology and Biotechnology Letters
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• v.29 no.3
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• pp.142-148
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• 2001

An indigenous antagonistic bacterium Bacillus sp. 7079 was isolated from a local soil sampled at Kyongju area in Korea . The strain has strong antagonistic ability which was originated from multifunctional mechanisms of chitinase and antibiotic and is a powerful antagonistic biocontrol agent against red-pepper rotting fungus Phytophthora capsici and Wilt fungus Fusarium oxysporum. The chitinase might degrade the cell wasll for Fusarium species. The selected Bacilus sp. 7079 was identified as a Bacillus amyloliquefaciens 7079. The maximal production of the chitinase from B, amyloliquefaciens 7079 were obtained in chitin-yeast extract medium containing 0.7%, $K_2$$HPO_4$, $0.2KH_2$$PO_4$, 0.1% ($NH_4$)$_2$$SO_4$, 0.05% sodium cirate, 0.01% $MgSO_4$$7H_2$O, 0.1% yeast extract and 0.1% colloidal chitin after cultivation of 3 days at pH 7.0 and $30^{\circ}C$. The best carbon and nitrogen sources for the production of the chitinase from B amyloliquefaciens 7079 were determined to be 0.1% colloi- dal chitin and 0.15% proteose peptone NO 3 respectively, The antagonistic activity of B amyloliquefaciens 7079 was confirmed using P. capsici by in vivo pot test with red-pepper plant.

• Environmental and Resource Economics Review
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• v.13 no.4
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• pp.717-734
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• 2004

This paper examined the statistical goodness-of-fit tests for biological growth model in bioeconomic analysis. Some authors estimated usually growth function for fish in the world. However, few studies have estimated growth equations for the bivalve species. Thus, this paper studied the common functional forms of fitting growth equations for cham scallops considering environmental factors and production styles. The following functional forms are considered: linear, log-reciprocal, double log, polynomial and linear with interactions. Results of fitting these various functional forms with real data are compared and evaluated using standard statistical goodness-of-fit tests. Results also indicate that log-reciprocal function is statistically the best fit to the real data. Therefore, the log-reciprocal function is decided the best function describing cham scallop biological growth and hence might be useful for economic evaluation(i.e., optimal harvesting time).

• Journal of Korean Society of Environmental Engineers
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• v.36 no.4
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• pp.265-270
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• 2014

Protease producing microorganisms were isolated from many kinds of food waste and fermented foods, which contains high amount and variable kinds of degraded substances. Several microorganisms were identified by 16S rRNA full sequencing analysis methods. The activity of protease was analyzed and identified in variable conditions for the application. For industrial use for biowaste treatment some proteases were isolated, identified and selected from microbial cells. And the tests were carried for the further use. The protein degrading activity at low temperature is useful for the treatment of organic waste, which contains much proteins. By the protein degradation process the organic waste can be utilized in variable fields, for example from feedstuff supplement to fertilizer for agriculture. Bacterial cells with protease activity at low temperature were isolated and identified. The optimal conditions for microbial cultivation and protease production were studied.

• Korean Journal of Medicinal Crop Science
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• v.11 no.5
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• pp.358-363
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• 2003

ScopoIamine and hyoscyamine which belong to tropane alkaloids are the pharmaceutically valuable anticholinergic drugs. In order to increase the productivities, the effects of elicitation were investigated during hairy root cultures of Scopolia. parviflora. Biotic elicitors originated from 3 fungi and 1 yeast were prepared as homogenate and supernatant and added to 3-week-old cultures. Both of homogenate and supernatant of Candida albicans elicitors increased the scopolamine production. The production of hyoscyamine was enhanced by homogenate of Fusarium solani and supernatant of C. albicans. Most of the other fungal elicitors were also improved on the tropane alkaloid production compared to non-treatment. Among the elicitors tested, C. albicans was proved the optimal biotic elicitor on tropane alkaloids production. These results will be served mass production of tropane alkaloids by large-scale production.

• Korean Journal of Agricultural and Forest Meteorology
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• v.1 no.2
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• pp.151-159
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• 1999

지구규모의 대기-지표-생물권(Atmo-Geo-Bio-Sphere)간의 끊임없는 상호작용(Interaction)의 피드백(Feedback)의 결과로 야기되는 기상변화는 아직도 이해를 위한 우리의 노력이 더욱 요구되고 있는 자연현상이다. 지구상의 모든 생물은 궁극적으로는 환경변화에 순응하여 생태계의 안정을 유지하는 방향으로 진화할 것이므로 생물자원의 관리분야라 할 수 있는 농업생산도 결국 기상자원의 변화에 효율적으로 적응하기 위한 인간활동인 것이다. 최근 빈발하고 있는 세계적인 이상기상의 출현은 일시적인 현상이 아니라 과도한 인간활동에서 야기된 대기질의 악화에 기인하는 비가역적이고 비선형적인 현상이라는데 불확실성과 심각성이 매우 크다. 지구상의 모든 농업생태계는 기상의 영향을 크게 받을 뿐만 아니라 반대로 기상에 커다란 영향을 미치는 중요한 생물권의 일부로 인식되고 있다. 농업활동의 결과는 항상 크던 작던 간에 대기조성에 영향을 미치게 되고 이는 다시 상호작용과 피드백에 의해 기상과 기후를 변화시키는 동인이 될 수 있다. 지구환경변화와 농업과의 관계는 지금까지는 주로 "기후변화가 농업생산에 미치는 영향"에 대한 접근방법을 택하여 왔다. 여기에서는 이와는 달리 "농업활동이 기후변화와 변동에 미칠 수 있는 가능성과 잠재력"에 대한 접근방법을 이용하여 농업과 기상과의 상호작용과 피드백효과를 새로운 시각에서 조견하고자 하였다. 상호작용 중 먼저 기후학적 변화원인으로 관심대상이 되고 있는 농업생태계의 온실기체 방출과 고정의 측면에서 이와 연관성이 높은 농업활동별로 온실가스의 방출/고정에 미치는 영향을 소개하고, 다음으로 생물지리화학적 및 생물지리물리학적 해석 방법을 검토 소개하였다. 한편 농업활동에 따른 지표면 특성의 변화가 국지기상 변화에 미치는 영향에 대한 검토를 에너지수지와 수분수지 관점에서 소개하였으며, 끝으로 농업활동의 근거지인 농촌지역의 인문사회학적 변화와 농업활동간의 관계, 그리고 이들이 기후변화/변동에 미칠 수 있는 잠재적 영향력을 살펴보았다.수 있는 잠재적 영향력을 살펴보았다.