• Korean Journal Plant Pathology
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• v.14 no.2
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• pp.145-149
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• 1998

Four nonpathogenic isolates of Fusarium oxysporum isolated from spinach showed suppressive effect on the occurrence of the Fusarium wilt of spinach caused by F. oxysporum f. sp. sprinaciae, among which NF01 controlled the disease most effectively. And NF01 was not pathogenic to tomato, cucumber, radish and spinach. This isolate was further tested for the biological control of the disease. The isolate was not inhibitory to the growth of the pathogen on potato sucrose agar medium, however the Fusarium wilt disease occurred less by drenching spore suspension of the nonpathogenic isolate. The control effect of the isolate was higher at lower inoculum level of the pathogen than at the higher inoculum level, and in the pretreatments than the simultaneous treatment of the isolate with the pathogen inoculation. The nit mutants of the isolate were easily formed on chlorate containing media, and was reisolated selectively as nit mutant from infected soil and plants. The reisolation rate of the isolate as opposed to pathogen was high at preinoculated soil and plants relative to the simultaneous inoculation of the isolate with the pathogen.

• 한국문화재보존과학회:학술대회논문집
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• 2005.11a
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• pp.252-254
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• 2005

박물관 수장고와 전시실 내의 유기물 문화재는 실내공기에 부유하고 있는 먼지와 곰팡이로 인해 생물적 피해를 받는다. 미생물로 인한 피해를 줄이기 위하여 박물관에서는 환경 조절법을 시행하거나 수장고와 전시실 내에 보존약제를 놓거나 또는 훈증 처리를 통한 방균 방제처리를 시행하고 있다. 미생물의 발생은 문화재 표면의 착색, 재질 분해, 변질 등을 야기한다. 본 연구는 위에 제시된 방법 외에 공기청정기를 이용하여 실내 부유 미생물 수를 줄여 유기물 문화재의 피해를 줄일 수 있는지를 알아보았다. 국립문화재연구소 내 생물과학실의 실험실 한 곳을 임의로 지정하여 공기청정기를 설치하기 전 곰팡이의 수를 측정하고, 공기청정기를 설치한 후의 곰팡이 변화를 4일 간격으로 측정하였다. 또한, 공기 중의 ???r성분 분석을 위하여 가스크로마토그래피(Gas Chromatography)를 이용하였다. 측정결과 곰팡이의 수는 대체적으로 감소하였으며, 채취한 곰팡이를 배양하여 동정을 실시하였다. 곰팡이 동정은 현미경을 통한 형태학적 분류와 ITS(Internal Transcribed Sequence)를 이용한 rDNA(Ribosomal DNA) PCR(Polymerase Chain Reaction)분석 두 가지 방법을 이용하여 동정하였다.

• The Korean Journal of Mycology
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• v.40 no.2
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• pp.118-123
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• 2012

In order to develop environment friendly fungicide for the control of citrus green mold (Penicillium digitatum) using endophytic bacteria, the 21 bacterial isolates were isolated from citrus leaves in seven different orchards in Jeju Province. Among the 21 bacterial isolates, 5 bacterial isolates presented antifungal activity against green mold pathogen P. digitatum. The CB3 isolate, which showed the most strong antagonistic effect, was selected through opposite culture against the pathogen. The rod-shaped, gram-positive bacterium CB3 was identified as Bacillus velezensis based on morphological, physiological characteristics, 16S rDNA, and gyr A gene sequence analysis. The isolate CB3 showed strong antifungal activity against two citrus postharvest pathogen P. digitatum. Citrus fruits were treated by wound inoculation with P. digitatum pathogen, and the control efficacy of CB3 culture broth was 66.7% ($1{\times}10^8$ cfu/ml). In conclusion, The stability of CB3 and its strong antifungal activity also lead us to believe that it has potential for application as an environment friendly biological control agent.

• The Korean Journal of Mycology
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• v.43 no.4
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• pp.253-259
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• 2015

Anthracnose caused by Collectotrichum acutatum is the most devastating disease of pepper plants in Korea. In this study, we evaluated the effect of selected antagonistic bacteria on control of anthracnose and plant growth promotion of pepper plants under field conditions. Four different bacterial isolates used in the current study were isolated from the pepper rhizosphere (GJ01, GJ11) and tidal flat (LB01, LB14) in previous studies. Four bacterial isolates, together with a control strain (EXTN-1), showed antifungal activity against C. acutatum in a dual culture assay. To test for plant growth promotion effect, seedling vigor index and growth parameters of pepper were measured under field condition. As a result, all four bacterial isolates were effective for improving plant growth promotion. The strain GJ01 was the most effective in improving the seedling vigor on pepper, but the strain GJ11 in increasing the pepper fruit yield. The incidence of anthracnose was inhibited in the range of 63.2~72.5% by treatment of four bacterial isolates. The current study indicated that the four bacterial isolates could be used as potential biological control agents of anthracnose disease of pepper.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.2
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• pp.105-111
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• 2013

The experiments were carried out to investigate the biocontrol potential of Lysobacter capsici YS1215 on root-knot nematode and to characterize its lytic enzyme activities. L. capsici YS1215 showed chitinase and gelatinase activities on the medium containing 0.5% chitin or 0.5% gelatin as substrates. Cell growth of L. capsici YS1215 was highest at 6 days, and the highest activities of chitinase (4.0 unit $ml^{-1}$) and gelatinase (7.43 unit $ml^{-1}$) were observed on 3 and 5 days after incubation, respectively. To investigate the effect of L. capsici YS1215 on tomato growth and nematode infection, the plants in pot trial were treated with bacterial culture (BC), half of bacterial culture (HBC), only bacterial medium (BM), tap water (TW) and commercial nematicide (CN). HBC treatd plants showed the higher shoot fresh weight and dry weight on $5^{th}$week after incubation while BM, HBC and BC had consistently higher values than TW at $9^{th}$ week. HBC appeared to be the highest shoot fresh length at $9^{th}$ week. Both CN and BC showed lower number of egg mass, root gall, and population of juveniles in soil compared to BC, HBC, BM and TW. These results suggest that L. capsici YS1215 with its strong ability of lytic enzyme production can be one of the most significant candidates for biocontrol agents against root-knot nematodes.

• The Korean Journal of Pesticide Science
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• v.12 no.1
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• pp.97-101
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• 2008

For the isolation of Actinomycetes showing nematocidal activity against Pine wood nematode, Bursaphelenchus xylophilus, about 2000 culture broth of Actinomycetes were tested and their activity were compared with that of Streptomyces avermitilis resulting a selected strain AW050027. The cultural, morphological and physiological analysis was performed for the identification of a selected strain. Phylogenetic analyses based on 165 rDNA gene sequences showed that the selected strain AW050027 belonged to the genus Micromonospora and M. corioriae $NAR01^T$ was the closest neighbors, sharing 98.9% 165 rDNA gene sequence similarity.

• Korean journal of applied entomology
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• v.39 no.4
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• pp.251-258
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• 2000

These studies were conducted to investigate the prey consumption and suppression of cotton aphid and green peach aphid by Chrysopa pallens Ramber as a predator. The 3$^{rd}$ instar of C. pallets fed on 29.8, 77.9, 133.6, and 155.7 individuals of apterous Aphis goussypii Glover a day at 17,22, 27, and 32$^{\circ}C$, respectively. A preovipositing female fed on 73.1 individuals, ovipositing female on 86.6 individuals, and adult male on 69.7 individuals of apterous Myzus persicae (Sulzer) a day at the 27$^{\circ}C$. The functional response curve of the larvae and adults of C. pallens to the densities of A. gossypii indicated Helling’s Type II: the consumption of prey by the C. pallens increased with the prey density but the consumption rate decreased. The attack rate of 3rd instar of C. pallens was the highest among the 2nd instar, 3$^{rd}$ instar, adult male and adult female, and handling time was the shortest. The critical ratio of the predator vs. the prey to effectively suppress the population of A. gossypii by releasing C. pallets eggs was 1 : 4 on red pepper and egg plant, and 1 : 3 on cucumber. Release of second larval stave of C. pallens at the ratio of 1 : 30 of the predator vs. the prey controlled satisfactorily A. gossypii on red pepper, and 1 : 20 on cucumber and tomato. The three-times introduction of the eggs of C. pallens was as effective as four applications of insecticides from mid-June to late September.r.

• Research in Plant Disease
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• v.19 no.1
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• pp.21-24
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• 2013

White rot caused by Sclerotium cepivorum was reported to be severe soil-born disease on garlic. Disease progress of white rot of garlic (Allium sativum L.) was investigated during the growing season of 2009 to 2011 at Taean and Seosan areas. The white rot disease on bulb began to occur from late April and peaked in late May. The antifungal bacteria, Burkholderia pyrrocinia CAB08106-4 was tested in field bioassay for suppression of white rot disease. As a result of the nucleotide sequence of the gene 16S rRNA, CAB008106-4 strain used in this study has been identified as B. pyrrocinia. B. pyrrocinia CAB080106-4 isolate suppressed the white rot with 69.6% control efficacy in field test. These results suggested that B. pyrrocinia CAB08106-4 isolate could be an effective biological control agent against white rot of garlic.

• Research in Plant Disease
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• v.19 no.1
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• pp.12-20
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• 2013

To isolate antagonistic bacteria against sclerotinia rot of lettuce, caused by Sclerotinia sclerotiorum, soil samples were collected from the diseased greenhouse field in Namyangju city, Gyeong-gi province from 2007 to 2008. A total of 196 bacterial isolates were isolated using serial dilution method. In dual culture assay in vitro, 26 isolates showed more than 80% of inhibition rates of mycelial growth of S. sclerotiorum. Based on 16S rDNA sequence analysis, the 26 isolates were identified as Bacillus megaterium, B. cereus, B. subtilis, Arthrobacter nicotianae, A. ramosus, Pseudomonas filiscindens, Stenotrophomonas maltophilia, Brevibacterium frigoritolerans and Sphingobacterium faecium. The 26 isolates inhibited the mycelial growth of S. sclerotiorum up to 80% and the sclerotial germination 0-100%. In the greenhouse pot test of ten isolates conducted in summer, 2 isolates B. megaterium (DK6) and B. cereus (C210) showed control efficacy on sclerotia viability of S. sclerotiorum, 20% and 35%, respectively. In the greenhouse pot test in winter, the disease incidence of the control group was 80%, whereas those of 9 isolates among 26 were approximately 20%. From the result, the 9 isolates are expected as potentially antagonistic bacteria for biological control of sclerotinia rot of lettuce caused by S. sclerotiorum.

• Research in Plant Disease
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• v.28 no.1
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• pp.1-18
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• 2022

Volatiles exist ubiquitously in nature. Volatile compounds produced by plants and microorganisms confer inter-kingdom and intra-kingdom communications. Autoinducer signaling molecules from contact-based chemical communication, such as bacterial quorum sensing, are relayed through short distances. By contrast, biogenic volatiles derived from plant-microbe interactions generate long-distance (>20 cm) alarm signals for sensing harmful microorganisms. In this review, we discuss prior work on volatile compound-mediated diagnosis of plant diseases, and the use of volatile packaging and dispensing approaches for the biological control of fungi, bacteria, and viruses. In this regard, recent developments on technologies to analyze and detect microbial volatile compounds are introduced. Furthermore, we survey the chemical encapsulation, slow-release, and bio-nano techniques for volatile formulation and delivery that are expected to overcome limitations in the application of biogenic volatiles to modern agriculture. Collectively, technological advances in volatile compound detection, packaging, and delivery provide great potential for the implementation of ecologically-sound plant disease management strategies. We hope that this review will help farmers and young scientists understand the nature of microbial volatile compounds, and shift paradigms on disease diagnosis and management to aromatic (volatile-based) agriculture.