• Proceedings of the Korean Society of Crop Science Conference
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• 2020.11a
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• pp.148-148
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• 2020

• Korean Journal of Microbiology
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• v.55 no.2
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• pp.160-163
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• 2019

Senegalimassilia sp. KGMB 04484 was isolated from fecal samples obtained from a healthy Korean. The whole-genome sequence of Senegalimassilia sp. KGMB 04484 was analyzed using the PacBio Sequel platform. The genome comprises a 2,748,041 bp chromosome with a G+C content of 61.18%, 2,300 total genes, 2,139 protein-coding gene, 21 rRNA genes, and 51 tRNA genes. Also, we found that strain KGMB 04484 had some genes for hydrolysis enzyme, fatty acid biosynthesis and metabolism in its genome based on the result of genome analysis. Those genes of KGMB 04484 may be related to regulation of human health and digest.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.114-114
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• 2022

Excessive endogenous endotoxin, especially lipopolysaccharide (LPS) reflux from gastrointestinal (GI) tract to the liver tissue is one of the most serious reasons of severe and acute liver injury which is mainly mediated by Kupffer cell activations. However, there is no clear molecular clues to explain the exact pathophysiological mechanism and effective drugs available till nowadays. We aimed to comprehend the pathophysiological features of LPS-induced liver injury and evaluate the efficacies of potential therapeutic drug, Ga-mi-Yuk-Mi-Jihwang-Tang (GYM), which is composed of herbal plants. GYM remarkably caused to normalize hepatic inflammation and oxidations against LPS-induced liver injury by evidence of serum liver enzymes, histopathological analysis, both hepatic protein and gene expression levels of pro-inflammatory cytokines, nitric oxide levels, and hepatic tissue levels of reactive oxygen species (ROS) levels, malondialdehyde (MDA), and 4-hydroxyneoneal, respectively. To assess molecular events in the hepatic tissue, we further found hepatic Sirtuin6 (Sirt6) levels were considerably depleted by LPS injection with aberrant alterations of Nrf2/HO-1 signaling pathways, whereas administration with GYM notably exerted to normalize these abnormalities. Our results exhibited that GYM would be one of target drug to diminish hepatic inflammation as well as oxidative stress by regulation of hepatic Sirt6 levels.

• Korean Journal of Plant Resources
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• v.16 no.3
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• pp.169-180
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• 2003

Biodiversity is defined as totality of genetic, species, and ecosystem variability. It provides natural sources of crop improvement, traditional medicine and biotechnology. In 1993, the Convention on Biological Diversity(CBD) became a legally binding framework for conserving and utilizing global biological diversity. It recognizes national sovereign rights over all genetic resources, such as the need to compensate developing countries for the resources they have provided to the industrialized world. The CBD grants access to those resources in exchange for compensation as well as technology transfer, so that the access to genetic resources would be made under prior informed consent(PIC) and mutually agreed terms(MAT). On the other hand, the developed countries argued that unfettered exchange of genetic resources was essential for scientific research and development, and that technology using genetic resources should be protected. There are many countries today, developing legal frameworks concerned with access to their local genetic resources and benefit sharing. In this study, we analyzed the international trends for conservation of biodiversity and sustainable use of genetic resources, and suggested how to cope actively with the situation.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.17-17
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• 2020

나고야의정서 발효에 따라 이에 대한 대응이 전세계적인 이슈가 되면서 각 국가들은 자국내 유용자원식물의 주권확립을 위한 움직임을 보이고 있으며 이들의 기원종 확립과 진위 감별을 위한 연구가 활발히 진행되고 있다. 이에 본 연구에서는 전라도 지역 내 생약 자원식물의 분포현황 및 실체를 파악하고자 하였다. 조사시기는 2018년 3월부터 2020년 5월까지 계절별로 해안가, 산지, 농경지 등 다양한 서식환경에 대하여 조사를 실시하였다. 전라도 내에서 확인되 생약자원식물은 106과 243속 405종 31변종 4품종 총 440분류군이 분포하는 것으로 조사되었으며, 한반도 관속식물 4,499분류군(Korea National Arboretum, 2018; Http://www.nature.go.kr/kpni/)의 9.8%에 해당된다. 본 조사지역 내에서 확인된 생약자원 식물 중, 특산식물로는 매미꽃, 할미밀망, 노랑붓꽃 등 11분류군이 확인되었으며, 희귀식물로는 CR등급의 노랑붓꽃을 포함하여, VU등급의 약난초, 흑삼릉 등 25분류군이 확인되었다. 특히 본 조사를 통해 수집된 생약자원식물의 표본, 화상자료, 종자, 생약부위 등은 추후 우리나라의 생약자원에 대한 관리DB를 구축하는데 기초자료로서 유용하게 활용될 것이다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.125-125
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• 2022

식물 내에는 배당체 형태의 생리활성 물질들이 다량 존재하고 있으며, 비배당체로 전환하였을 경우 생리활성이나 투과성이 증대된다는 연구들이 보고되어 있다. 따라서 본 연구에서는 고활성 β-glucosidase 활성을 갖는 균주를 선별함으로써 식물 내의 비배당체 활성 성분을 증대시키는 데 활용하고자 한다. 균주는 전남지역 제조된 막걸리부터 분리하였다. 분리된 균주는 성장 속도 등 산업적으로 활용될 수 있는 가치를 평가하여 11종 22점의 균주를 분리하고, 16S rRNA분석을 통해 균주를 동정하였다. 동정된 균주를 이용하여 β-glucosidase의 활성을 평가하여 고활성 균주를 1종을 선별하였다. 선별된 균주는 다른 균주에 비하여 상대적 활성이 57% ~ 99%정도 활성이 높았으며, 공시 균주와 비교하였을 때도 상대 활성이 높은 았다. 선별된 균주의 특성을 규명하기 위하여 glycerol, L-arbinose를 비롯한 49가지의 탄수화물의 대사 활성을 확인해본 결과 25가지의 탄수화물 대사 활성을 가지고 있는 것을 알수 있었다. 또한 Lipase, β-glucosidase 19가지의 다양한 효소활성을 확인해본 결과 8종의 효소활성이 있음을 확인할 수 있었다.

• Korean Journal of Microbiology
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• v.54 no.4
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• pp.456-459
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• 2018

The genus of Olsenella has been isolated from vertebrate animal mouth, rumen, and feces. Olsenella sp. KGMB 04489 was isolated from fecal samples obtained from a healthy Korean. The whole-genome sequence of Olsenella sp. KGMB 04489 was analyzed using the PacBio Sequel platform. The genome comprises a 2,108,034 bp chromosome with a G + C content of 65.50%, 1,838 total genes, 13 rRNA genes, and 52 tRNA genes. Also, we found that strain KGMB 04489 had some genes for hydrolysis enzymes, and antibiotic biosynthesis and resistance in its genome based on the result of genome analysis.

• Bulletin of Korea Environmental Preservation Association
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• s.390
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• pp.4-5
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• 2010

• Journal of Bio-Environment Control
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• v.32 no.1
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• pp.81-83
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• 2023

• Journal of Marine Bioscience and Biotechnology
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• v.15 no.1
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• pp.1-11
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• 2023

In the present study, the antioxidant and anti-inflammatory activities of ethanolic extracts from Lathyrus japonicus at concentrations of 50, 100, and 200 ㎍/mL were investigated in LPS-stimulated, RAW264.7 cells. Antioxidant properties were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays and ferric reducing antioxidant power assay. In addition, the production of reactive oxygen species (ROS) was measured using the 2',7'-dichlorofluorescein diacetate (DCFH-DA) probe by flow cytometry. To examine the anti-inflammatory activity of the extracts of L. japonicus, their effects on the levels of nitric oxide (NO); production of cytokines such as interleukin (IL)-1β, IL-10, and tumor necrosis factor-α (TNF-α); and the activities of enzymes such as inducible NOS (iNOS) and cyclooxygenase-2 (COX-2) were assessed. The IC₅₀ values of the DPPH and ABTS radical scavenging assays were 476.09 ± 1.50 and 34.91 ± 0.37 ㎍/mL, respectively. In addition, L. japonicus extracts not only inhibited ROS production, but also the production of NO, IL-1β, and IL-10, and the activity of iNOS in a dose-dependent manner. In summary, the ethanolic extracts of L. japonicus could be used as a functional food additive and an anti-inflammatory agent owing to their antioxidant and anti-inflammatory activities