• Journal of Adhesion and Interface
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• v.20 no.2
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• pp.71-75
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• 2019

Tannic acid is one of the most commonly found polyphenols in the vegetable field. Initially, research on tannins concentrated on physiological functions such as antioxidants. Recently, however, tannic acid has attracted much interest as a molecular glue as it has been found to interact virtually all bio-macromolecules such as proteins and DNA. The various properties of tannic acid are expected to control the wettability of the surface, contribute to energy storage and generation, and show potential as a medical agent. Here, tannic acid will be discussed about the interaction of with bio-macromolecules as a molecular glue, surface modification, and utilization of itself as biomaterials.

• Korean Journal of Agricultural Science
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• v.34 no.2
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• pp.99-109
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• 2007

Cellulomanas sp. CS 1-1 was studied for its morphological, physiological and biochemical characteristics, together with DNA homology and fatty acid pattern to elucidate its taxonomical position in the species level. Colony morphology of CS1-1 exhibited circular form, opaque, convex, entire edge and pale yellow. Cells were of rod with the size of $0.3{\sim}0.5{\times}0.8{\sim}1.2{\mu}m$, while coryneforms were formed at the early stage of culture. D-ribose, raffinose, rhamnose, acetate, propionate, L-lactate, D-gluconate, aspartate and proline were not utilized as a sole source of carbon, whereas saccharose, arabinose, and amlyose were utilized. Biochemical characteristics of CS1-1 were Gram positive, catalase positive, oxidase negative, nonmotile, facultative anaerobic, mesophilic and G+C content of 74.7 mol %. The major fatty acid and menaquinone were 12-methyltetradecanoic acid(anteiso-$C_{15:1}$) and MK-$9(H_4)$, respectively. These results were correspondent with the characteristics reported for member of the genus Cellulomonas. The strain CS 1-1 exhibited a high level of DNA homology as 70% with C. uda ATCC491, compared to those of 54~59% with C. fimi ATCC 15724, 46~48% with C. biazotea, C. gelida and C. bibula. Finally, strain CS1-1 could be classified as a novel species belongs to C. uda.

• Proceedings of the KOSOMBE Conference
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• v.1997 no.05
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• pp.203-206
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• 1997

본 연구는 G7 의료공학기술개발사업의 1차년도 연구결과이다. 본 연구의 최종목표는 청각계통중 내이의 손상에 의한 감각성 난청환자에게 소리를 인식할 수 있게 하는 한국형 인공와우의 개발, 전 음성 난청환자의 음성인식 효율을 높일 수 있는 한국형 인공이소골의 개발 그리고 인공와우 시술환자에게 적합한 재활용 프로그램을 개발하는데 있다. 이를 위하여 지난 1차년도에는 코클리어의 전기생리학적 현상을 규명하고, 이식전극시스템과 그 주변장치를 설계, 평가하였다. 그리고 한국인의 성인사체의 16귀를 측정하여 한국인 체형에 적합한 인공이소골의 설계기준을 확립하였다. 또한, 인공와우 시술 후 청력회복에 필요한 한국형 재활프로그램의 개발을 위하여 인공와우의 coding strategy에 따른 언어분별력 조사, 유소아의 어음특성분석등을 행하였다. 이 연구결과를 토대로 차기년도에는 상용화에 근접한 프로토타잎의 제품개발과 임상실험이 진행되리라 생각된다.

• Phonetics and Speech Sciences
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• v.2 no.2
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• pp.37-42
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• 2010

The purpose of this study is to first discriminate and assess those infants who appear healthy in appearance but who could face possible risk factors in the future and, secondly, to identify those infants who may have difficulties in their developmental stages. The subjects of this study consisted of 35 full-term infants (39-40 weeks) and 33 pre-term infants (34-35 weeks). The infants' voices were recorded for three minutes, for which EDIROL by Roland and a stand-type microphone made by SONY were used. This was done to discern the value of the Breath unit (B-unit) and the fundamental frequencies ($F_0$). It was found that there were significant differences in terms of F0 since the pre-term infants had higher F0 than the full-term infants, showing a result of 436.4 Hz for the full-term infants and 460 Hz for the pre-term infants (p<.05) There was an average rate of 4.01 for the full-term infants and 4.02 (SD=1.69) for the pre-term infants in shimmer. For NHR, it was observed .44 for the full-term infants and .50 for the pre-term infants, thus revealing no significant differences in these observations. This study shows that the crying of newborn babies is related to their physical conditions and it is a sensatory response to these conditions. Furthermore, this study could be helpful for the early detection and measurement of newborn babies who look clinically healthy but could be at risk through acoustic and physiological analyses.

• Journal of Game and Entertainment
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• v.2 no.2
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• pp.61-69
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• 2006

Along with advance in visual culture, the importance of motion graphic has been increasingly emphasized day by day, which can maximize information delivery using image illustration and typography, graphic factors of images. In addition, we can easily see increasing cases where what a designer intends is visualized using creative typography in diverse mass media such as TV commercials, movies or web. Thanks to the effects of this trend, various ways of manufacturing works have been proposed in the field of motion typography by applying diverse factors including verbal ones, time, form, motion, colors, and sound for the purpose of expressing formless semantic notions through visual form of typography. However, physiological features such as sex, age, health status, pathological conditions, and body size can have a bigger effect on the process of real communication. Therefore, if property of quasi-verbal sound can be reflected appropriately in motion typography where communication is expressed only by visual form, it can enable people to understand what a designer intends faster and more exactly.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.1
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• pp.46-53
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• 2004

In this study, we compared the levels of methylotrophic bacterial community diversity in the leaf, stem, grain, root and rhizosphere soil sainples of four rice cultivars collected from three regions of Korea. Thirty five pigmented and five non-pigmented isolates showing characteristic growth on methanul were obtained. When phylotypes were defined by performing numerical analysis of 42 characteristics, four distinct clusters were formed. While two clusters, I and IV diverged on the basis of nitrate and nitrite reduction, other two clusters, comprising only pink pigmented colonies, diverged on the basis of cellulase activity. Out of the two reference strains used in the analysis, Methyhbacterium extorquens AM1 diverged from all the clusters and M. fujisawaense KACC 10744 grouped under cluster III. All the isolates were positive for urease, oxidase, catalase and pectinase activity and negative for indole production, MR and VP test, $H_2S$ production, starch, and casein hydrolysis. No clusters were found to possess thermotolerant isolates, as no growth of the isolates was observed at $45^{\circ}C$. Two strains in cluster I were found to possess gelatin hydrolysis and methane utilizing properties respectively. Most of the isolates in all the four clusters utilized monosaccliarides, disaccharide and polyols as carbon source. Six isolates showed considerable nitrogenase activity ranging from 86.2 to $809.9nmol\;C_2H_4\;h^{-1}\;mg^{-1}$ protein.

• Korean Journal of Microbiology
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• v.48 no.4
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• pp.270-274
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• 2012

Three actinobacterial strains exhibiting broad spectrum antibiotic activities were isolated from soil, and characterized. Through the comparative analysis of 16S rRNA genes, the three isolates could be assigned to the genus Streptomyces, as S. tanashiensis, S. nashivillensis, and S. rubiginosohelvolus were found to be the mostly related species, but the strains formed independent phylogenetic lineage. Each strain exhibited different antimicrobial profile against Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus, Gram-negative bacteria Salmonella typhi, Enterobacter cloacae, Serratia marcescens, and Pseudomonas aeruginosa, and also fungi Candida tropicalis and Candida krusei. In addition to the antimicrobial profile, the strains also differed in API ZYM test results, which implies that the three strains might produce difference antimicrobial substances.

• Korean Journal of Microbiology
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• v.32 no.4
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• pp.252-257
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• 1994

A new hydrogen sulfide-oxidation bacterium, Thiobacillus sp. was isolated from waste coal mine water around Hawsun in Chunnam province. The isolate was motile gram-negative rod shape, formed spore and grew up to be aerobically facultative chemolithotroph by using energy released from the oxidation of reduced inorganic sulfur compounds. It could assimilate various kinds of organic compounds and grew well upon thiosulfate-supplemented basal medium. To the lelvel of 32 mM in thiosulfate concentration, thiosulfate in itself was utilized as energy source for growth. However, from those of the higher concentration than 32 mM, thiosulfate functioned specifically as the substrate inhibitor rather than as the energy source. It was found that the optimum thiosulfate concentration for growth was 32 mM. The G+C content of the DNA was 65.0 mol%. The isolate had 16 : 1 + 17$_{cyc}$, 16 : 0 as their major non-hydroxylated cellular fatty acids, 3-OH 12 : 0 as a hydroxylated fatty acid and also contained unidentified $C_{18}$ branched fatty acid. The ubiquinone system in the respiratory chain was Q-9. Based on the physiological and biochemical characteristics, the isolate was assigned to a novel species of the genus Thiobacillus sp. iw.

• Journal of Life Science
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• v.19 no.8
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• pp.1177-1182
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• 2009

In order to establish in vitro infection model for research of plant pathogen based on tissue softening disease in napa cabbage, eighty independent bacterial strains were isolated from the softened napa cabbage tissues. Eight bacterial isolates were primarily screened with the generation of reproducible tissue softening disease to fresh napa cabbages within 24${\sim}$48 hours after inoculation. Through various microbiological biochemical and morphological examinations, three Gram (-) isolates which harbor independent biological properties were finally chosen, and named as RBI, RB2 and RB6. Collective results obtained from API 20E test and analyses of VITEK 2 COMPACT and nucleotide sequences of 165 rRNA of each isolate proposed that isolates RBI and RB2 are close to the Erwinia carotovora subsp. odorifera, and RB6 is close to the Erwinia carotovora subsp. carotovora. These isolates grew optimally at $30^{\circ}C$ with neutral pH culture condition. The isolates caused softening tissue disease with dose-dependent manner regardless of pre-surface damages of napa cabbage. Minimum dose to cause soft rot disease for RBI, RB2 or RB6 were $8.0{\times}10^8$ CFU/mt $10^9$ CFU/ml or $4.7{\times}10^6$ CFU/ml respectively. These isolates caused tissue softening disease to eggplant, paprika and napa cabbage out of 14 different tested vegetables, indicating that these isolates damages specific plant tissues. The bacterial isolates obtained in this research and in vitro plant infection model will be adapted in the understanding of the mechanism of pathogenesis by plant pathogen.

• Journal of Life Science
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• v.18 no.4
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• pp.482-487
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• 2008

In this study, we screened and identified the bacterial strain showing high alkaline pretense inhibitor activity from marine environment. Nine bacterial strains with alkaline pretense inhibitor activity were isolated from marine sediments. Among them, strain C12 had the highest alkaline pretense inhibitor activity and was selected for further taxonomical study. On the basis of morphological and physiological characteristics, strain C12 was identified as the genus Streptomyces. A phylogenetic analysis of the 165 rDNA showed that the isolated strain was actually a member of the genus Streptomyces, because the determined sequence exhibited a higher homology with Streptomyces thermocarboxydus. Morphological characteristics showed cylindrical spore chain and smooth spore surface by scanning electron microscope. Strain C12 was grown on all media except for ISP 9 agar. This strain could be grown in the medium containing up to 9% NaCl.