Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Isolation and Characterization of Streptomyces spp. from Soil Showing Broad Spectrum Antibiotic Activity

광범위한 항균활성을 보이는 토양 유래 Streptomyces 속 방선균의 분리 및 특성 연구

  • Park, Sewook (Department of Microbiology and Molecular Biology, Chungnam National University) ;
  • Bae, Taeok (School of Medicine, Indiana University) ;
  • Kim, Seung Bum (Department of Microbiology and Molecular Biology, Chungnam National University)
  • Received : 2012.11.16
  • Accepted : 2012.12.12
  • Published : 2012.12.31
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Abstract

Three actinobacterial strains exhibiting broad spectrum antibiotic activities were isolated from soil, and characterized. Through the comparative analysis of 16S rRNA genes, the three isolates could be assigned to the genus Streptomyces, as S. tanashiensis, S. nashivillensis, and S. rubiginosohelvolus were found to be the mostly related species, but the strains formed independent phylogenetic lineage. Each strain exhibited different antimicrobial profile against Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus, Gram-negative bacteria Salmonella typhi, Enterobacter cloacae, Serratia marcescens, and Pseudomonas aeruginosa, and also fungi Candida tropicalis and Candida krusei. In addition to the antimicrobial profile, the strains also differed in API ZYM test results, which implies that the three strains might produce difference antimicrobial substances.

토양시료로부터 광범위한 항생작용을 보이는 방선균 3개 균주를 분리하여 그 특성을 조사하였다. 분리주의 16S rRNA 유전자 염기서열 비교 분석을 통해 3개 분리주는 모두 Streptomyces 속에 속하고, S. tanashiensis, S. nashivillensis 및 S. rubiginosohelvolus와 근연 관계에 있는 것으로 나타났으나 독립적인 계통을 형성하여 신종으로서의 가능성을 보여주었다. 항균활성 검정 결과 세 균주는 Bacillus subtilis, Staphylococcus aureus 등의 그람 양성세균, Salmonella typhi, Enterobacter cloacae, Serratia marcescens, Pseudomonas aeruginosa 등의 그람 음성 세균, 그리고 Candida tropicalis 및 Candida krusei 등의 진균류에 대해 각각 서로 다른 길항작용을 보였다. 또한 세 균주 간에는 생리학적 활성에도 차이가 나타나 각 균주가 서로 다른 항생물질을 분비할 가능성이 있음을 보여주었다.

Keywords

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