• Title/Summary/Keyword: 색소 생성

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Algal Pigments and their Degradation Products in Suspended and Sinking Particulate Material in the Gulf of Mexico (멕시코 만의 부유 및 침강고형물에 존재하는 조류색소 및 그 분해산물)

  • Noh, Il
    • Journal of Environmental Science International
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    • v.5 no.2
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    • pp.153-170
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    • 1996
  • Photosynthetic pigments and their degradation products in suspended and sinking particles collected from the Gulf of Mexico waters 1987~88, were measured using High Performance liquid Chromatography (HPIC). The short term variations in flux rates of chlorophylls and carotenoids as well as-their degradation products were compared at the mesoscale cyclonic and anticyclonic circulation features (cold joie rink and warm core ring). Chlorophyll a was the predominant porphyrin of suspended particulate matter at both CCR -and WCR. Among carotenoid pigments, 19'-hexanoyloxyfucoxanthin, which is a biomarker of prymnesiophytes, was dominant pigment at both rinds. Phaeophorbide a, which is produced through the Brazing processes of grazers, was the predominant degraded pigment in sinking particles ai the study aiea. Total pigment flux in CCR was an order of magnitude higher than that in WCR. Less than l% of the standing stock of the pigments measured sank out of the upper 200m of the WCR on any given day. Thus, suspended particulate matter in Gulf of Medico was not recycled rapidly.

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Pigment-forming bacteria in the presence of L-typrosine and their possible role in the browning of fermented soybean products (대두발효식품의 갈변과 관련된 티로신산화 세균에 관한 연구)

  • Park, Seung-Kyu;Kyung, Kyu-Hang
    • Korean Journal of Food Science and Technology
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    • v.18 no.5
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    • pp.376-381
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    • 1986
  • A hypothesis that Korean home-made fermented soybean products are brown-pigmented in large part by contaminated bacteria is proposed. Twenty six strains of bacteria forming brown pigments in the presence of L-tyrosine were isolated from home-made soybean paste. They were characterized and all were identified as strains of Bacillus subtilis. The isolates produced dark brown to brownish black pigmentation on yeast extract-peptone-glucose agar (YPGA) supplemented with 0.1% L-tyrosine in 72 hours but not on YPGA. They also caused different depress of lighter pigmentation on potato dextrose agar and nutrient agar. When an arbitrarily chosen pigmenting isolate was cultivated in a liquid medium supplemented with L-tyrosine, it began to produce pigments only after cell growth stopped. The tyrosinase enzyme was extracted and the enzyme activity was measured by using L-tyrosine and 3-hydroxytyrosine (L-dopa) as substrates. The crude enzyme preparation porduced pigments at rates of $2.1\;{\times}\;10^{-3}\;and\;5.0\;{\times}\;10^{-3}$ optical density units/min measured at 490㎚ for tyrosine and dopa, respectively. Possible content of L-tyrosine in a soybean paste formula was calculated.

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Studies on the fluorescent Pseudomoηas isolated from the wheat rhizosphere (소맥근권(小麥根圈)에서 분리(分離)한 형광성(螢光性) Pseudomonas spp. 에 관(關)한 연구(硏究))

  • Lee, Myung-Chol;Kim, Yong-Woong;Kim, Kwang-Sik
    • Korean Journal of Soil Science and Fertilizer
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    • v.23 no.2
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    • pp.152-159
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    • 1990
  • Four active strains, producing siderophore and antagonizing to soil plant disease fungi, were isolated and identified from the wheat rhizosphere to reduce the injurious effects of continuous cropping and study on the biological control. The obtained results were summerized as follow. 1. Four strains of fluorescent Pseudomonas were isolated from the wheat rhizosphere and identified as Pseudomonas fluorescens Biotype A(Ps-1,2,5) and Biotype B(Ps-3) That strains inhibitied growth of R. solani, F.oxysperum and F. solani in vitro 2. Optical density of pigment was maximum at 410nm. 3. Siderophore production by identified strains was decreased with addition of $Fe^{+3}$, although not decreased with addition of $Fe^{+2}$ 4. Pigment of Ps-1, 2 and 3 strain inhibitied growth of R. slani, F. oxysperum and F. solani but pigment of Ps-5 strain did not inhibitie growth of R. solani 5. Effect of inoculation was in order of Ps-2, 1, 5 and 3 strain through the dark culture method, and effect of Ps-1 and Ps-2 was greater than that of treatment of captan 50ppm.

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Enhancement of Skin Color by Postharvest UV Irradiation in 'Fuyu' Persimmon Fruits (수확 후 자외선 조사에 의한 '부유' 단감의 과피 착색 증진)

  • Choi, Seong-Jin
    • Horticultural Science & Technology
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    • v.29 no.5
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    • pp.441-446
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    • 2011
  • The effects of UV irradiation, as a hormetic stimulus, on the postharvest persimmon fruits (Diospyros kaki cv. Fuyu) were investigated in regards to the change of carotenoid contents and flesh softening, when the UV irradiation was combined with or without the pretreatment of 1-methylcyclopropene (1-MCP) as an ethylene action inhibitor. The major carotenoid pigments in persimmon fruits were ${\beta}$-carotene, lycopene and ${\beta}$-cryptoxanthin. Of them, the lycopene was a pigment, which increased markedly after harvest. UV irradiation increased the contents of ${\beta}$-carotene and lycopene, enhancing the skin color to scarlet. The treatment accelerated however also the softening of fruit flesh. But the softening of UV irradiated fruits could be delayed significantly by pretreatment with 1-MCP without reducing the advantageous effect of UV irradiation on the carotenoid increase.

Growth Characteristics and Changes of Pigment Content of Purple Sweet Potato during Growth (자색고구마의 생육특성 및 색소함량의 변화)

  • Kim, Seon-Jae;Rhim, Jong-Whan;Lee, Lan-Sook;Lee, Joon-Seol;Jeong, Byeong-Choon
    • Korean Journal of Food Science and Technology
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    • v.28 no.6
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    • pp.1180-1183
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    • 1996
  • Growth characteristics and changes of pigment content of purple sweet potato (PSP) during growth were investigated. Vine length of PSP was increased to the maximum length of 130 cm after 100 days of growth. Numbers of branches and nodes were increased to the maximum of 21and 550, respectively, after 120 days of growth. Vine weight was also increased to the maximum of 4,384 kg/10a after 120 days of growth, while the weight of marketable root was increased continuously to the end of the growth period of 150 days to reach the production of 1,875 kg/10a. Uniquely considerable amount of anthocyanin pigment has been developed in both skin and flesh of young roots harvested after 40 days of growth. The pigment content was increased slowly until 140 days of growth then decreased. This fact indicates that the best time for harvesting of PSP is 140 days after planting.

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Studies on Albinic Flat-Fish Paralichthys olivaceus I. Effects of Enzyme Activities and Substrates on Melanin Formation (넙치의 백화현상 규명에 관한 연구 I. 멜라닌 색소 생성에 미치는 효소와 기질의 영향)

  • Choi Yeung Joan;Kang Seok-Joong;Cho Chang-Hwan;Myoung Jung-Goo;Kim Jong-Hyun
    • Journal of Aquaculture
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    • v.3 no.2
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    • pp.155-165
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    • 1990
  • The albinic phenomenon of flat-fish (Paralichthys olivaceus) was investigated by measuring protein content, tyrosinase activity, amino acid composition, and contents of vitamin A and C. These materials in the flat-fish feed-stuff were also tested. The amount of skin protein was higher than that of muscle in normal flat-fish. Catechol and L-dopa oxidase activity did not differ between normal and albinic flat-fish. The free amino acid of skin in normal flat-fish was 7.5 times that in albinic one. Sulfur-containing amino acid in normal flat-fish was also 6.3 times that in albinic ones. Vitamin A was not detected in both of flat-fish. The content of vitamin C in normal flat-fish was 7.8 times that in albinic one. The contents of protein, sulfur-containing amino acid and vitamin C in micro-encapsulated feed (one commercial feed in Japan) were the highest among the feed-stuff used in this experiment. The melanin formation of flat-fish skin was affected by substrates such as aromatic amino acid and cofactor such as sulfur amino acid.

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Dyeing effects of natural pigment from marine bacterium, Microbulbifer sp. PPB12 (해양세균 Microbulbifer sp. PPB12 생성 천연색소의 섬유 염색 효과)

  • Lee, Ga-Eun;Park, Jin-Sook
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.3
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    • pp.527-533
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    • 2017
  • As the clothing industry has advanced, dyeing technologies using various dyes have been developed. In recent years, interest in natural pigments has been increasing because of the negative impact of synthetic pigment on human health; therefore, development and application of microbial pigments is demanded. In this study, the dyeing effects on multifiber fabrics and biological activity were assessed using violet natural pigment from the marine bacterium, Microbulbifer sp. PPB12. The violet pigment produced by cultivation of Microbulbifer sp. PPB12 using Marine broth 2216 for 3 days was extracted using ethanol. Once dissolved in 20% ethanol, the violet pigment could be used to dye bleached cotton, diacetate, and especially polyamide. The optimal temperature, time, pH, and bath ratio under the dyeing conditions were $80^{\circ}C-90^{\circ}C$, more than 1 hour, pH 4-6, and 1:25, respectively. The mordant treatment was more suitable for color expression when $Na_2SO_4$ was used after 10 minutes of dyeing, but no significant difference was observed from untreated samples. The violet pigment also showed antibacterial activity against B. subtilis. The results of the present study indicate that the marine bacterial pigment could be an alternative for textile dyeing as a natural dye with antibacterial activity.

Changes in chemical properties, antioxidant activities, and cytotoxicity of turmeric pigments by thermal process (가열처리에 의한 심황색소의 화학적 특성, 산화방지 활성 및 세포독성 변화)

  • Song, Eiseul;Kang, Smee;Hong, Jungil
    • Korean Journal of Food Science and Technology
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    • v.50 no.1
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    • pp.21-27
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    • 2018
  • Turmeric oleoresin, extracted from the rhizome of Curcuma longa L., is a widely-used natural food colorant. Curcuminoids, the major pigments in turmeric, which include curcumin, demethoxycurcumin (DMC), and bisdemethoxycurcumin (BMC), possess various physiological activities. In the present study, changes in the chemical properties, antioxidant activities, and cytotoxicity of turmeric pigments upon heating were investigated. Color intensity of turmeric was significantly reduced after heating. Residual levels of curcumin, DMC, and BMC after 15 min of heating at $95^{\circ}C$ were 11.9, 37.4, and 77.3% respectively. Scavenging activities of turmeric against 2,2'-azobis-3-ethyl-benz-thiazoline-6-sulfonic acid (ABTS), 2,2-azobis (2-amidinopropane) hydrochloride (AAPH) peroxyl radicals, and nitrite were significantly enhanced after heating, while 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging activity remained unaffected. Generation of $H_2O_2$ from turmeric was increased via thermal decomposition. Cytotoxicity of turmeric pigments against colon cancer and normal intestinal cells was reduced significantly after heating. The results indicate that thermal processing affects chemical properties and bioactivities of turmeric pigments. These effects should be considered during the processing of foods containing turmeric pigments.

곰팡이 RF101이 생성하는 적색 색소의 안정성 및 추출물의 생리활성 검정

  • Yu, So-Hyeon;Yang, Deok-Jo
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.329-332
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    • 2001
  • The stability of red pigment isolated from RF101 was determined over a period of storage for the pH, light, temperature and various metal ions. The absorption maximum of red pigment was 510nm. Red pigment was stable from pH 5 to pH 9 and in dark conditions. It was also stable against temperature below $25^{\circ}C$ and in $K^+$, $Ca^{2+}$, $Mg^{2+}$, Antibacterial activity of RF101 showed growth-inhibitory activity against plant pathogens Agrobacterium spp.

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Characterization of Pigment-Producing Kocuria sp. K70 and the Optimal Conditions for Pigment Production and Physical Stability (색소생성 균주 Kocuria sp. K70의 특징과 색소생성 최적 조건 및 물리적 안정성)

  • Kim, Young-Sook;Park, Jin-Sook
    • KSBB Journal
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    • v.25 no.6
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    • pp.513-519
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    • 2010
  • Marine bacterium producing pigment was isolated from the solar saltern of Mijo-myeon, Namhae, Korea. Based on phenotypic characteristics and 16S rRNA sequence analysis, the strain was identified as Kocuria sp., which produced a yellow pigment. The pigment showed UV absorption maximum at 469nm. The bacterial strain grew well on Marine broth 2216 culture medium. Productivity of the pigment reached the maximum value after 44 hours at $30^{\circ}C$, 2% NaCl and pH 6.0. The pigment was produced best when supplied by 1% lactose as a carbon source and 1% beef extract as a nitrogen source. The result of the color stability study showed that pigment extracted from the strain by ethanol was stable at $-20-25^{\circ}C$ and also showed higher stability over 70% for 14 days in light conditions at $25^{\circ}C$. The pigment extract was also stable for all metal ions tested, except for $FeCl_2$.